60 results about "Collard Green" patented technology

The present invention relates to a diet composition comprising brown rice, job's tear, red beans, buckwheat, Italian millet, Indian millet, white beans, black beans, black rice, barley, brown rice sprouts, kale, matured pumpkin (Curcurbia moschata), carrot, Angelica, cabbage, wild Lanceolate root, Lotus root, radish, green radish, mugwort, pine needles, laver, sea mustard, sea tagle, Shiitake mushroom, Reishi mushroom, soy peptide, isolated soy protein, L-carnitine, Hibiscus extract, green tea extract and solomon's seal extract. The composition is useful in reducing body weight and body fat; controlling body shape; and lowering serum cholesterol and neutral fat.

The invention particularly relates to a method for preparing collard berry yoghurt and a product thereof, which solves the problems of single nutrition and poor mouth feel of the prior yoghurt and lutein functional product. In the method, collard, pineapple and raw milk are taken as main raw materials and the method comprises the following steps: preparing a fermenting agent, collard powder and pineapple berry, then mixing the collard powder, the pineapple berry and the raw milk, fermenting and curing after cold storage so as to obtain the product, wherein the collard powder is added in the preparation process of the fermenting agent. The invention has the advantages that the nutrition and the functions of collard, pineapple particles and yoghurt are effectively compounded, the colour of the collard berry yoghurt is light green and natural, the collard berry yoghurt has a favorable tissue state, has compact and smooth quality, moderate viscosity, sweet and sour taste and aromatic flavour and is soft and smooth, and the product is a purely natural product, is rich in the effective components of lutein, Vc, lactic acid and the like and does not contain any preservative.

The invention discloses a culture method for a brassica oleracea L. var. acephala microspore regeneration plant. The method comprises the following steps: taking inflorescences of brassica oleracea L. var. acephala and rape, directly taking or taking after induction alabastrum of the inflorescences, adding the alabastrum into NLN-13 induced medium after disinfection to form fluid suspension, carrying out filtering, and centrifuging the filtrate to obtain a precipitate; adding NLN-13 induced medium and active carbon mixed liquor in order so as to obtain a microspore fluid suspension; carrying out a heat shock treatment, followed by culturing so as to obtain embryoids in cotyledon stage; inoculating the embryoids to embryoid differential medium until the embryoids are differentiated and regenerates into buds; cutting the regenerated buds to a rooting medium for rooting culture, and hardening and transplanting seedlings to obtain regenerated plants; taking young leaves of the regenerated plants for the detection of ploidy of corresponding regenerated plants and determining regenerated seedlings of rape and brassica oleracea L. var. acephala in the regenerated plants. According to the method provided in the invention, rape which is easy to generate embryos and brassica oleracea L. var. acephala which is difficult to generate embryos are mixedly cultured; the material which is easy to generate embryos is used to spur the material which is difficult to generate embryos; therefore the ratio of embryos of brassica oleracea L. var. acephala is improved.

Specific plant extracts are applied topically to upregulate genes which code for proteins to prevent the build-up of and mitigate the activity of various harmful materials within the skin. These upregulated proteins include glutathione transferases, peroxiredoxins, oxidoreductases, glutaredoxins and glutathione syntheses. These proteins produce additional glutathione, glyoxalase I, maintain cellular redox homeostasis, reduce hydrogen peroxide and alkylhydroperoxides, and conjugate glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Keratinocytes have been induced to significantly upregulate glutathione and glyoxalase I protein expression. Additionally, keratinocytes pre-treated with diallyl trisulfide have increased viability when exposed to ozone, UV radiation or cigarette smoke extract. The active material can be extracted from plants such as garlic, onions, shallots, leeks, chives, scallions, brussel sprouts, broccoli, cabbage, cauliflower, bok choy, kale, mustard, turnip or radish, may include any compound of the allyl sulfide family, and may be of synthetic origin.

The invention provides two SSR labels that located on both sides of the brassica oleracea red leaf gene Re and are closely linked to the gene, i.e. C9Z90 and C9Z94. The genetic distances between the two labels and the Re gene are 0.3cM and 2.0cM respectively. According to the nucleotide sequences of the two labels, two pairs of specific amplification primers are designed respectively for molecule assisted selection of brassica oleracea white leaf gene. In the assistant selection process, the two labels can be used at the same time, and have the characteristics of good repeatability, high reliability, low detection cost, and time saving and labor saving.

The invention relates to a processing method for an ornamental kale dry product, comprising the following steps of: picking raw materials, cleaning, sterilizing, slicing, drying in a baking oven, separating, carrying out sampling observation, freezing, packaging and warehousing. The method provided by the invention is used to greatly improve the defect that fresh kale borecole is not easy to store for a long time and effectively adjust the case that the kale borecole can not be eaten due to reasons such as season and producing area. By the adoption of the processing method, the ornamental kale dry product is convenient to eat, can recover if immersed into clear water when eaten, and can be cooked according to a routine method.

The present invention discloses a purple yam and white fungus nourishing and life tonifying noodle and a preparation method thereof. The noodle is composed of the following raw materials: flour, purple yams, white fungus, wheat germ flour, brassica juncea var. multisecta, Brazil nuts, ginkgo nuts, kale, konjac flour, longans, chaenomeles speciosa, begonia fimbristipula, melissa officinalis powder, fresh rhizoma phragmitis, tendrilleaf solomonseal rhizome, sophora japonica buds and coffonrose hibiscus leaves. Compared with the existing noodles, the purple yam and white fungus nourishing and life tonifying noodle has a unique production process and a scientific material choice, and specially adds a variety of natural and health-beneficial edible ingredients and traditional Chinese herbs. The finished products are rich in protein, vitamins and minerals, and can enhance human immune function and maintain human health when people enjoy the taste of delicious food.

The invention discloses a method for culturing cabbage type rape with long ornamental period by collard. The method comprises steps of hybridizing a regular oilseed rape type with various collards; performing double cultivation on a culture base containing 20 mg/L colchicine at a F0 seed obtained through distant hybridization; after seedling, rooting and transplanting to a big farmland; if the hybridized F1 contains target characteristics, continuing the backcross transformation with cabbage type rape; after the backcross descendant reaches the characteritics of the cabbage type rape, quickly and stably performing microspore culture when the fertility is normal; hybridizing various leaf colour materials with successful transformation with cabbage type rape, selecting a new type of cabbage type rape with different leaf and flower colors; matching and planting new types of various cabbage type rape with different leaf and flower colors to meet people's demand of enjoying leaves in winter and flowers in spring. Through distant hybridization, related gene of collard leaf color is transferred to the cabbage type rape; meanwhile, through the combination of leaf color and flower color, the ornamental period of the ornamental oilseed rape is prolonged.

The invention discloses three gene segments (sequences are respectively shown in the 1st-936th nucleotide in SEQ ID No.1, SEQ ID No.2 and SEQ ID No.3) of collard DWARF, SP and GA20ox, and an RNAi (ribonucleic acid interference) carrier of three genes of collard DWARF, SP and GA20ox built on the basis of the segments. The RNAi carrier is transformed into collard through agrobacterium; and expression of the three genes of the collard DWARF, SP and GA20ox in a transgenic positive plant are obviously reduced in comparison with that of a non-transgenic plant, and the plant type is miniaturization phenotype. Thus, new miniature collard product is obtained by total silence of the three genes of the DWARF, SP and GA20ox. The RNAi carrier has good market prospect and economical value, and meanwhile, consultation and reference are provided for miniaturization breeding of other plants.

The invention belongs to the technical field of agricultural planting and particularly relates to a high-yield and high-quality cultivating method for brassica oleracea var.acephala in a plant factorycondition. The high-yield and high-quality cultivating method for the brassica oleracea var.acephala in the plant factory condition comprises the steps of (1) sowing: saturating a sponge block with clear water once, sowing seeds of the brassica oleracea var.acephala into the sponge block, irrigating the sponge block with the clear water, carrying out shading, and putting the sponge block on a seedling frame of a seedling area for seed sprouting; (2) seedlings separation: removing a shade after the seeds sprout, transferring the seedlings after sprouting together with the sponge block into a target pinch plate in the plant factory condition and then putting the pinch plate into a nutrient solution seedbed to ensure that all roots are immersed into a nutrient solution; (3) permanent planting: when each plant has 4-5 leaves after seedlings separation, transferring and permanently planting all plants in the pinch plate in first seedling separation into the other pinch plate and putting the other pinch plate into the nutrient solution seedbed; and (4) harvesting: when each plant expands 10-12 outer leaves in 20-25 days after permanent planting, picking tender leaves for eating.

The invention belongs to the technical field of genetic breeding of plants, and specifically relates to a cultivation method of a novel germplasm of colorized brassica oleracea L.var.capitata. The cultivation method is characterized in that parents of the cultivation method comprise brassica oleracea L.var.capitata and ornamental kale; the male parent is the brassica oleracea L.var.capitata, and the female parent is the ornamental kale; or, the female parent is the brassica oleracea L.var.capitata, and the male parent is the ornamental kale; the ornamental kale is b.oleracea L.var.acephala DC.; and more preferably, the genotype of the male parent is GGSSHH, and the genotype of the female parent is RRCChh; or, the genotype of the male parent is RRSShh, and the genotype of the female parentis GGSSHH. According to the invention, when the breeding germplasm is selected, the b.oleracea L.var.acephala DC. with strong ornamental property and cold-resistant property is selected, and the brassica oleracea L.var.capitata with sweet and soft mouth feel, and good balling property and ball shape and suitable for uncooked eating is selected as a parent material, so that the cultivated colorizedbrassica oleracea L.var.capitata has the characteristics that the color variety is rich; the color is bright; the ornamental property is strong; the cold-resistant property is strong; the eating quality is good; the mouth feel is sweet and crisp; the colorized brassica oleracea L.var.capitata is suitable for uncooked eating; and the balling property is relatively good.

The invention discloses a method for quickly culturing homozygous transgenic ornamental cabbages with insect resistance and herbicide resistance. The method for culturing the transgenic cabbages comprises the following steps of: immersing the cotyledons of the cabbages by using recombinant agrobacterium tumefaciens containing a target gene and then co-culturing to obtain the transgenic cabbages. In the invention, the inductivity of the adventitious buds of explants after conversion treatment reaches 48 percent in the selection of a culture medium, the number of regeneration buds on each explant is averagely 1 to 2, and the conversion frequency of the regeneration buds reaches 35 percent by the validation of molecular identification. The result of the manual feeding of the larvae of diamond back moths proves that the obtained transgenic regeneration plant has insect resistance, and the larva lethality of a best resistant material can reach 97 percent. The process from the conversion of the explants to the acquisition of the homozygous transgenic resistant material only needs a transient time of three years.

The invention discloses a method for breeding a male sterile line and a maintainer line of kale. The method mainly comprises the following steps: selecting a kale variety; determining a breeding goal after the first generation of selfing; directionally selecting a single plant and performing the second generation of selfing; directionally selecting a single plant from selfing progenies and continuing to self; directionally selecting the single plant according to the breeding goal; meanwhile, performing interspecific hybridization with a cytoplasmic male sterile line as a female parent and the selected single plant as a male parent to obtain hybrid F1, so that the CMS (cytoplasmic male sterility) gene of the sterile line is transferred to the kale; purifying by continuing to self the selfed progenies; meanwhile, screening the sterile plant, which is closest to the male parent breeding goal, from cytoplasmic male sterile hybrid plant population to serve as the female parent, screening the single plant, which is closest to the breeding goal, from the selfed progenies as the male parent, and continuing to perform hybridization in pairs; and purifying the selfed progenies and transferring the male sterile line of each generation in pairs at the same time, thus obtaining the maintainer line and the male sterile line after selfing for a plurality of generations. By the method, labor and cost can be reduced remarkably, and breeding time can be shortened.

The invention discloses a breeding method of supersulcus-leaf Brassica oleracea var. acephala f. tricolor Hort., which comprises the following steps: carrying out inbred purification on collard species 'white peacock' to obtain white partite-leaf collard inbred line Y316, and hybridizing by using Y316 as a male parent and cabbage cytoplast male sterile material C-12 as a female parent; backcrossing with the original male parent by selecting an individual plant, which has the closest characters with the male parent, from filial generation as the female parent; repeating the second step, and continuously backcrossing to obtain white partite-leaf collard male sterile line CMS316; and carrying out inbred purification on the collard species 'red pigeon' to obtain red roundleaf collard inbred line Y438, and hybridizing by using Y438 as the male parent and the white partite-leaf collard male sterile line CMS316 as the female parent to obtain the supersulcus-leaf Brassica oleracea var. acephala f. tricolor Hort. hybrid. The new hybrid species obtained by the method disclosed by the invention has the advantages of high ornamental value, long ornamental period, cold resistance and disease resistance.

The invention relates to a preserved fruit food, in particular to a collard date fruit flake, which solves the comprehensive utilization problem of the collard and date. The collard date fruit flake is made from 4-6 parts of red date, 1-3 parts of the collard, agar which accounts for 1.2-1.5% of the total weight of the red date and the collard, beta-cyclodextrine which accounts for 0.15-0.20% of the total weight of the red date and the collard, and L-ascorbic acid which accounts for 0.035-0.045% of the total weight of the red date and the collard. The collard date fruit flake has complete appearance, good elasticity and toughness, smooth surface and homogeneous structure; the collard date fruit flake is chocolate brown in terms of color and has glossiness; the collard date fruit flake has moderate sweetness, smoothness and exquisite taste with regard to mouth feeling and flavor; and the collard date fruit flake has red date scent without odor.

The invention discloses a spleen-invigorating and qi-replenishing licorice-flavor mutton shashlik. The spleen-invigorating and qi-replenishing licorice-flavor mutton shashlik is characterized by being prepared from the following raw materials in parts by weight: 500-550 parts of mutton, 2-3 parts of anise, 3-4 parts of pepper, 5-6 parts of Chinese Angelica powder, 6-7 parts of collard, 7-8 parts of Scirpus tabernaemontani, 8-9 parts of powdered milk and 3-4 parts of oleum morrhuae. The added licorice has the efficacies on invigorating spleen and replenishing qi and clearing away heat and toxic materials; Lysimachia christinae Hance is also added and has the efficacies on reducing fever, causing diuresis and relieving rheumatic pains; and auxiliary materials with specific taste such as collard, Scirpus tabernaemontani and Plumeria rubra are added, so that the mutton shashlik has good mouthfeel and is suitable for people of all ages.

The invention discloses a method for preventing and controlling color pepper thrips in a sunlight greenhouse. Color peppers are cultivated in the sunlight greenhouse according to large and small rows,the large row spacing is 80cm, and the small row spacing is 70cm; Brassica oleracea var. acephala f. tricolor is transplanted 10-12 days before field planting is performed on the color peppers, a rowof Brassica oleracea var. acephala f. tricolor is planted in the middle of small rows of field planting rows, and the plant spacing is 30cm; plug seedling raising is performed on the Brassica oleracea var. acephala f. tricolor by adopting a substrate containing carbonized rice husks, carbonized rice husks are applied additionally into field planting holes, a dinotefuran liquid pesticide is adopted to dip hole trays and root irrigation is performed; 1-2 days before field planting is performed on the color peppers and 35 days after field planting is performed, a mixed aqueous solution of a repelling plant extract and chitin is sprayed to leaf surfaces. The method provided by the invention utilizes the trapping plant and the directional concealment application technology to kill the thrips before the thrips spread to the color peppers, at the same time, the use amount of chemical pesticides can be reduced, the non-contact chemical prevention and control of mainly-planted crops can be realized, and food safety and ecological safety can be guaranteed.

The invention discloses a method for preventing and controlling tomato spotted wilt viruses. The method comprises the steps: cultivating tomatoes in a sunlight greenhouse according to large and smallrows, wherein a large row spacing is 80 cm, and a small row spacing is 60 cm; transplanting collards within 10-12 days before the tomatoes are planted, planting a row of collards between small rows ofa planting row, wherein the row spacing is 25 cm; carrying out plug seedling on the collards by using a substrate containing carbonized rice hulls, additionally applying the carbonized rice hulls into planting pits, and dipping plug trays and irrigating roots by using a dinetofuran liquid; and before transplanting the collards, sealing a ventilation opening of the sunlight greenhouse by using a 40-mesh insecticidal protection net. By using the method, the incidence rate of the tomato spotted wilt viruses can be effectively reduced on the premises that the dosage of a chemical pesticide is greatly reduced and the non-contact chemical prevention and control of main cultivated crops are realized, and generally, the incidence rate of the spotted wilt viruses of susceptible tomato varieties can be controlled within 5%.

The invention discloses a high-yield cultivation method of kale. The method comprises the following steps: sowing: selecting a flat site as a seedbed in the late summer and early autumn, uniformly laying a layer of substrate on the seedbed, watering, carrying out hole tray sowing and seedling raising, and transplanting and planting in the field: one month after sowing, when kale seedlings grow four leaves and a core, planting the kale seedlings in the field, applying decomposed human and livestock manure, and carrying out routine field management; cutting off tops: six months after transplanting and field planting, when the kale starts bolting, cutting off bolting stems and top leaves, maintaining the height of the bottom of kale at 18-22cm; spraying mist to the plants after cutting off tops, spraying mist to the plants once after aligning and cutting off tops. As for the high-yield cultivation method of kale, the bolting stems and top leaves are removed when the kale starts bolting, 300-fold diluted Bacillus thuringiensis solution is sprayed directly onto the plants and foliar fertilizer is topdressed. Thus, a lot of primary branches grow in the leaf axils of the kale, the harvesting period is effectively prolonged and can be extended to early May, and the yield is increased.