RNAi (Ribonucleic acid interference) carrier of three genes of collard DWARF, SP and GA20ox, and building method and application thereof

A kale and carrier technology, applied in the field of genetic engineering, can solve time-consuming and labor-intensive problems

Inactive Publication Date: 2013-04-03
CHONGQING UNIV
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Problems solved by technology

[0004] Although traditional breeding has made great contributions in the process of...

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  • RNAi (Ribonucleic acid interference) carrier of three genes of collard DWARF, SP and GA20ox, and building method and application thereof
  • RNAi (Ribonucleic acid interference) carrier of three genes of collard DWARF, SP and GA20ox, and building method and application thereof
  • RNAi (Ribonucleic acid interference) carrier of three genes of collard DWARF, SP and GA20ox, and building method and application thereof

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Embodiment Construction

[0051] Preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The experimental method that does not indicate specific conditions in the preferred embodiment is usually according to conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, etc., Science Press, 2002) conditions, or as recommended by the manufacturer.

[0052] The kale variety used in the preferred embodiment is Jinglianhong No. 1. The pMD18-T vector is the product of TaKaRa Company, the pGEM-T Easy vector is the product of Promega Company, pHANNIBAL vector, pBIN19 vector, Escherichia coli DH5α, Escherichia coli "helper" bacteria containing the swimming plasmid Prk2013, Agrobacterium tumefaciens LBA4404 maintained by the laboratory. RNA Extraction Kit, RNA PCR Kit (AMV) Ver.3.0 Kit, DNA Extraction Kit Universal Genomic DNA Extraction Kit Ver. 3.0, Lig...

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Abstract

The invention discloses three gene segments (sequences are respectively shown in the 1st-936th nucleotide in SEQ ID No.1, SEQ ID No.2 and SEQ ID No.3) of collard DWARF, SP and GA20ox, and an RNAi (ribonucleic acid interference) carrier of three genes of collard DWARF, SP and GA20ox built on the basis of the segments. The RNAi carrier is transformed into collard through agrobacterium; and expression of the three genes of the collard DWARF, SP and GA20ox in a transgenic positive plant are obviously reduced in comparison with that of a non-transgenic plant, and the plant type is miniaturization phenotype. Thus, new miniature collard product is obtained by total silence of the three genes of the DWARF, SP and GA20ox. The RNAi carrier has good market prospect and economical value, and meanwhile, consultation and reference are provided for miniaturization breeding of other plants.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and relates to a plant gene fragment, an RNAi carrier, a construction method of the RNAi carrier, and the application of the RNAi carrier in plant breeding. Background technique [0002] Kale is a biennial herbaceous plant. It is a horticultural variety of edible cabbage (cabbage, cabbage). Its leaf colors are rich and varied, and the leaf shapes are also different. ", is a good product for potted foliage. [0003] Plant architecture is an important agronomic trait of plants. Miniaturization, as an important aspect of the plant type of ornamental plants, has shown a good market prospect. For example, a special variety of modern roses—miniature roses are loved by people because of their short and compact plant type, small and cute leaves and flowers, small space, and greater choice of placement. One of the most popular potted flowers in the flower market. Therefore, cultivating kal...

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Application Information

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IPC IPC(8): C12N15/53C12N15/54C12N15/84C12N15/66C12N1/21A01H5/00
Inventor 胡宗利解巧利陈国平涂昀刘琴
Owner CHONGQING UNIV
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