39 results about "Increased Bone Density" patented technology

The invention relates to an application of plant lactobacillus Hi188 in promotion of bone health. The invention provides the application of the plant lactobacillus Hi188 or the afterbiogen thereof or the composition thereof in increasing the bone mineral density, increasing the bone mineral density, promoting the skeletal development, increasing the bone mineral content and / or promoting the tibia growth. Or preparing food for increasing bone mineral density, increasing bone mineral density, promoting skeletal development, increasing bone mineral content and / or promoting tibia growth; wherein the composition comprises: (A) Phytobacterium plantarum Hi188 or a postbiotic thereof, and (B) Bifidobacterium lactis Ca360 or a postbiotic thereof.

The application provides a preparation method of non-denatured type II collagen for increasing bone density and improving bone elasticity, and belongs to the technical field of biotechnology. The application adopts EDTA-citric acid buffer containing beta-mercaptoethanol to decalcify cartilage, adopts supercritical CO2 extraction technology to defat, adopts alkaline protease and collagenase II to perform matrix directional enzymolysis, and performs gradient digestion; then three-phase centrifugal separation is adopted to separate non-denatured type II collagen, and biomimetic H column chromatography purification is adopted, so that non-denatured type II collagen with high extraction rate, high purity and high structural integrity can be obtained.

This disclosure relates methods and compositions for preventing bone loss and increasing bone density and strength.

This invention belongs to the field of biotechnology, specifically relating to an osteogenic amino acid fragment Z01 and its screening and applications. This invention utilizes a unique cell screening platform specifically for Osx: the CL-Osx-EGFP cell line. This platform fuses Osx and EGFP, using EGFP as a tracking marker to directly reflect changes in Osx expression and screen for compounds that can activate Osx. Z01 was first discovered to effectively activate Osx, and its amino acid sequence is shown in SEQ ID NO.1. Pharmacokinetic and toxicological analyses were performed on Z01, and its osteogenic activity was verified in an ovariectomized rat osteoporosis model. Ultimately, it was demonstrated that Z01 possesses osteogenic activity, can improve bone microstructure, increase bone density, and has a wide range of applications.

The application discloses a composition for improving bone density and application thereof, and belongs to the technical field of food. The composition is prepared by using 1,3-dioleic acid-2-palmitic acid triglyceride, osteonectin and oligosaccharide in combination, and through the interaction between the components, the absorption and utilization of each other are promoted, the growth and development of the bone of an infant in the growth period are improved, the bone mineral content and bone density are improved, the bone health is promoted, and the composition is safe and has no side effect. Through optimization of the types of the oligosaccharide, it is found that the combination of oligogalactose and 2'-fucosyllactose can better improve the growth and development of the bone of the infant in the growth period.

This invention relates to a nutritional composition for promoting bone development and its applications. The invention provides applications of the nutritional composition or preparations comprising said nutritional composition, including: (1) preparing food products; (2) preparing health products for promoting bone development, increasing height, and / or improving bone density; (3) preparing drugs or drug compositions for promoting bone development, increasing height, increasing bone density, treating or preventing osteoporosis, and / or treating or preventing skeletal dysplasia; and (4) promoting bone development, increasing height, and / or improving bone density for non-therapeutic purposes; wherein said nutritional composition comprises medium- and long-chain triglycerides (MLCT) and 1,3-diunsaturated fatty acid-2-palmitoyltriglycerides (UPU).

The invention provides a micro-fat calcium supplement carrier, which is prepared from the following raw materials in parts by weight: 30 to 35 parts of standard index calcium, 1 to 5 parts of chondroitin, 1 to 5 parts of glucosamine, 8 to 12 parts of pure camel milk, 0.7 part of vitamin D, 2 to 70.3 parts of vitamin K, 1 to 3 parts of phospholipid complex and 8 to 10 parts of excipient. According to the invention, standard calcium, chondroitin, glucosamine, pure camel milk, vitamin D, vitamin K2-7, a phospholipid complex and an excipient are organically mixed by adopting a liposome carrier technology, so that the digestion and absorption of calcium in a body and the distribution of calcium in a specific region are effectively improved, and further the effects of rapid bone growth, increase of bone length (promotion of height increase) and increase of bone density are achieved; the health-care food has the health-care functions of reducing the fracture probability, preventing tooth decay, reducing the death rate of cardiovascular diseases and the like, so that the calcium absorption rate is remarkably improved and reaches up to 95%.

The invention relates to the technical field of collagen peptide extraction, and particularly discloses a preparation method of fresh bovine bone collagen peptide for increasing bone mineral density, which comprises the following steps: step S1, degreasing treatment; step S2, crude extraction; step S3, performing enzymolysis; step S4, performing supercritical extraction; according to the technical scheme, the extraction promoting liquid is added in the crude extraction process, meanwhile, ultrasonic-assisted synchronous microwave radiation is matched, calcium lactate, lecithin, chlorogenic acid and compound enzyme are added in the enzymolysis process, and the purity and yield of the fresh bovine bone collagen peptide are improved through the synergistic effect of all the procedures.

The application provides a compound preparation for improving osteoporosis and osteoarthritis, a preparation method and application thereof, and belongs to the technical field of biological medicines. The compound preparation contains the following raw materials: glutathione, curcumin, calcium carbonate, vitamin D3, hydrolyzed collagen, and glucosamine sulfate; wherein the glutathione exists in the form of a cyclodextrin inclusion compound. The preparation method comprises the following steps: mixing the calcium carbonate, the hydrolyzed collagen, the glucosamine sulfate and excipients, then adding an equal amount of a premix of the glutathione cyclodextrin inclusion compound, the curcumin and the vitamin D3 in a stepwise manner, and performing dry granulation and tabletting to obtain the compound preparation. The compound preparation can simultaneously increase bone density, protect articular cartilage, reduce the levels of oxidative stress and inflammation, and can be used for preparing medicines or health foods for preventing or adjuvant treating osteoporosis and osteoarthritis. The application has a scientific formula, a stable process and a good application prospect.

The application provides a composition for promoting bone health and bone formation, and application and products thereof, and belongs to the field of food. The composition of the application is composed of 2'-fucosyllactose, osteopontin and a calcium source, wherein the osteopontin is 1-5 parts, the 2'-fucosyllactose is 1-50 parts and the calcium source is 0.05-50 parts by weight. The composition of the application shows a good synergistic effect in promoting calcium absorption, and can be used for increasing bone density, preventing and treating osteoporosis and the like.

The invention discloses a device for treating osteoporosis by compounding electromagnetic field and ultrasonic wave with Disumab, which comprises a base plate, a support bed mattress plate is fixedly mounted on the upper surface of the base plate, a limiting groove is formed in the base plate, and a transmission assembly is movably arranged in the limiting groove. A medicine conveying module, an electromagnetic field generating module and an ultrasonic wave generating module are fixedly arranged on the surface of the transmission assembly, the medicine conveying module, the electromagnetic field generating module and the ultrasonic wave generating module are electrically connected with an external control module, and a display interaction module is arranged in the external control module; the electromagnetic field, ultrasonic waves and medicine assistance are combined, the synergistic effect of multiple treatment means is achieved, the treatment effect on osteoporosis is improved, and compared with a single treatment mode, the bone mineral density can be more rapidly and effectively increased, and the treatment period can be shortened.

The invention provides a bone collagen peptide for increasing bone mineral density and a preparation method thereof, the method comprises the following steps: S1, dispersing bone gelatin in water to obtain a bone gelatin solution; s2, adding a 0.1-1 mol / L acidic pH regulator into the bone gelatin solution at a speed of 1-10 mL / min until the turbidity of the system is increased by 10-20% compared with the turbidity of the initial bone gelatin solution, then stopping adding, standing for 10-30 min, and filtering to obtain a bone gelatin supernatant; s3, adding endoprotease into the bone gelatin supernate to obtain enzymatic hydrolysate; and S4, performing post-treatment and drying on the enzymatic hydrolysate to obtain the bone collagen peptide. According to the method, the mass ratio of the low-molecular-weight polypeptide in the obtained bone collagen peptide can be increased, and the composition of the obtained bone collagen peptide is more suitable for bone metabolism to increase the bone density.

The invention relates to a composition with a function of improving bone mineral density, application of the composition and a preparation method of related health-care products or foods, and the composition comprises the following components in parts by weight: 0.5-4.5 parts of D-glucosamine hydrochloride, 0.5-3 parts of calcium carbonate, 0.1-1.5 parts of sodium chondroitin sulfate, 0.5-2.5 parts of American ginseng extract, 0.2-2 parts of salvia miltiorrhiza extract, 0.15-1.5 parts of radix paeoniae alba extract and 0.1-1 part of turmeric extract. The composition is prepared into a medicine, a health care product or a food to increase the bone mineral density. According to the composition, green, natural and high-quality raw materials and auxiliary materials are selected, the composition is prepared from the perspective of traditional Chinese medicine, modern nutriology and modern pharmacology, calcium supplement, articular cartilage protection and inflammation relieving are integrated, the formula plays a comprehensive role in increasing the bone mineral density in different links, and the effect of increasing the bone mineral density under the effect of three pipes is achieved. The composition can be prepared into medicines, health-care products or foods, the preparation process is simple, and the prepared product is green, safe and remarkable in curative effect and has a good market prospect.

This application provides a collagen peptide for increasing bone density and a method for preparing the same. The method includes the following steps: S1: dispersing bone gelatin in water to obtain a bone gelatin solution; S2: adding 0.1-1 mol / L acidic pH adjuster to the bone gelatin solution at a rate of 1-10 mL / min until the turbidity of the system increases by 10%-20% compared to the initial bone gelatin solution, then stopping the addition, allowing it to stand for 10-30 min, and filtering to obtain a bone gelatin supernatant; S3: adding an endopeptide to the bone gelatin supernatant to obtain an enzymatic hydrolysate; S4: post-processing and drying the enzymatic hydrolysate to obtain collagen peptides. This method can increase the mass ratio of low molecular weight peptides in the obtained collagen peptides, resulting in a composition of collagen peptides more suitable for bone metabolism and utilization to increase bone density.

This invention discloses the application of PT-129 in the preparation of drugs for treating rheumatoid arthritis (RA), belonging to the field of RA treatment. This invention is the first to demonstrate that the compound PT-129 can target and intervene in the synovial microenvironment, effectively inhibiting the abnormal activation behaviors of RA fibroblast-like synovial cells (FLS) such as proliferation, migration, and invasion, and downregulating the expression of N-cadherin and MMP3 proteins in synovial tissue. This invention provides a combined drug formulation containing PT-129 and RGFP966. This composition exhibits a significant synergistic effect in vivo, effectively relieving joint swelling, increasing bone density and trabecular bone thickness, reducing trabecular bone separation, thereby inhibiting cartilage degradation and bone destruction. This invention provides a novel targeted drug and combination therapy with definite efficacy for the clinical intervention of RA, possessing extremely high clinical application development value.

This invention discloses a Hu sheep active peptide complex and its application in bone health care. Using healthy Hu sheep bones as raw material, collagen is extracted after defatting and decalcification. This collagen is then enzymatically hydrolyzed with trypsin and alkaline protease, followed by ultrafiltration, desalting, and freeze-drying to obtain Hu sheep bone collagen peptides. These collagen peptides are mixed with pharmaceutical excipients, calcium source compounds, chondroprotective agents, and fat-soluble vitamins. An ethanol-water binder solution containing palmitoyl aspartate diethyl ester and hesperidin-3-O-glucoside is added, and the mixture is granulated, dried, sized, and pressed to obtain the active peptide complex. This complex, through the synergistic effect of its components, can significantly promote osteoblast proliferation and inhibit apoptosis, effectively increasing bone density in osteoporosis model animals. It also exhibits excellent biocompatibility and no cytotoxicity, solving the problems of limited function, insufficient preparation efficiency, and instability of existing single active peptides. This makes it suitable for the development and application of bone health-related nutritional supplements.

The invention discloses a hydrolyzed egg yolk peptide capable of improving bone growth and bone mineral density and preparation and application of the hydrolyzed egg yolk peptide, the hydrolyzed egg yolk peptide is tetrapeptide SFPA, the amino acid sequence is Ser-Phe-Pro-Ala, and the hydrolyzed egg yolk peptide can be prepared through enzymolysis of degreased egg yolk powder. The tetrapeptide SFPA is a food-borne bioactive peptide, a zebrafish model verifies that the tetrapeptide SFPA has remarkable effects of promoting longitudinal growth of bones and increasing bone mineral density, the body length of zebrafish with normal growth and development is increased by 3.4% after intervention of the hydrolyzed egg yolk peptide, and the body length of zebrafish with slow growth is increased by 3.4% after intervention of the hydrolyzed egg yolk peptide. After intervention of the hydrolyzed egg yolk peptide, the body length is increased by 3.9%, and the bone mineral density is increased by 19.6%. The method can be used for preparing products beneficial to improvement of bone mineral density and / or improvement of bone growth.

The invention relates to the technical field of biology, in particular to collagen peptide for promoting osteoblast proliferation and differentiation and increasing bone density and a preparation method and application thereof. The method comprises the following steps: (A) soaking cartilage which is roughly divided into blocks by using an alkaline solution, and then cleaning the soaked cartilage; (B) removing grease in cartilage; (C) cleaning the cartilage from which the grease is removed by using an acid solution; (D) carrying out high-pressure heat treatment on the pickled cartilage, wherein microwave heating is adopted in the heat treatment; (E) step-by-step enzymolysis: firstly carrying out enzymolysis on the cartilage by using protease, and then continuously carrying out enzymolysis on the cartilage by using chondroitin sulfate ABC enzyme; and (F) purifying the enzyme-deactivated enzymolysis feed liquid by an alcohol precipitation method. The product obtained by the method has good acid resistance and is rich in small molecule chondroitin sulfate.

Disclosed are methods and compositions for restoring Cyr61 in bone tissue. In certain aspects, the compositions comprise a recombinant Cyr61 carried in carbon nanotubes. The compositions may be used for various methods including restoring Cyr61 expression in bone, increasing bone density, or treating a bone disease in a patient.

The application discloses a hydrolyzed egg yolk peptide for improving bone growth and bone density as well as preparation and application thereof. The hydrolyzed egg yolk peptide is a tetrapeptide SFPA with an amino acid sequence of Ser-Phe-Pro-Ala. The hydrolyzed egg yolk peptide can be prepared by enzymatic hydrolysis of defatted egg yolk powder. The tetrapeptide SFPA is a food-derived bioactive peptide. It is verified by a zebrafish model that the tetrapeptide SFPA has a significant effect of promoting longitudinal bone growth and increasing bone density. For a normally growing and developing zebrafish, the body length of the zebrafish is increased by 3.4% after intervention of the hydrolyzed egg yolk peptide. For a slow-growing zebrafish, the body length of the zebrafish is increased by 3.9% and the bone density is increased by 19.6% after intervention of the hydrolyzed egg yolk peptide. The hydrolyzed egg yolk peptide can be used for preparing a product for improving bone density and / or for improving bone growth.

This invention provides a probiotic, a probiotic composition, and their applications, all possessing the functions of calcium ion enrichment and vitamin K synthesis. This invention belongs to the field of microbial technology. The probiotic provided by this invention is *Lactobacillus bulgaricus* subsp. Lactobacillus delbrueckii subsp. bulgaricus NWP-101 or Pyrococcus lactis ( Pediococcus acidilactici NWP-299 has the advantages of efficient calcium enrichment, high vitamin K production and excellent probiotic properties. It can be used to prepare probiotic preparations, functional foods, health foods or dietary supplements to prevent or improve osteoporosis and increase bone density.

The application belongs to the technical field of biotechnology and tissue engineering, and particularly relates to application of an osteoporosis-reversing period deer blood polypeptide in preparation of an osteoporosis treatment preparation. The application first proposes and isolates and prepares an active substance of precisely applied deer blood, i.e., an osteoporosis-reversing period deer blood polypeptide RDBP, and provides a preparation method of the RDBP. The deer blood polypeptide obtained through the preparation method has a significant anti-osteoporosis prevention and treatment effect, and the effect is significantly stronger than that of deer blood polypeptides in other periods. The deer blood polypeptide has the characteristics of being natural, safe and free of side effects. The osteoporosis-reversing period deer blood polypeptide has important application value in the fields of preparation of medicines for preventing / treating osteoporosis or health care products and medical products for increasing bone density.

This invention relates to the field of small molecule peptide extraction, specifically a method for preparing and applying yak bone marrow protein peptides that increase bone density. It solves the problems of decreased protein activity during cryopreservation, incomplete enzymatic hydrolysis after polyphenol compounds bind to proteins, and low activity of small molecule peptides. The method includes using a special embedding material to embed polyphenol compounds, forming a protein protectant; ultrasonically dispersing the mixture of yak bone marrow and the protein protectant; freeze-drying and placing the mixture in an extraction solution; adding petroleum ether to the lower layer of the extraction solution for extraction, removing oil and the special embedding material in one step; then adding saturated ammonium sulfate; filtering and dissolving in water to obtain a high-molecular-weight protein active solution; ultrafiltration and enzymatic hydrolysis of the high-molecular-weight protein active solution to obtain a small molecule peptide solution; and further post-processing to obtain highly active protein peptides. This invention achieves convenient transportation of proteins after low-temperature refrigeration while maintaining higher activity of the enzymatically hydrolyzed small molecule peptides.

This application relates to the field of biotechnology, and particularly to collagen peptides that promote osteoblast proliferation and differentiation and increase bone density, as well as their preparation methods and applications. The method includes (A) soaking coarsely shaped cartilage in an alkaline solution, followed by washing the soaked cartilage; (B) removing grease from the cartilage; (C) washing the grease-removed cartilage with an acid solution; (D) subjecting the acid-washed cartilage to high-pressure heat treatment using microwave heating; (E) stepwise enzymatic hydrolysis, including first hydrolyzing the cartilage with a protease, followed by further hydrolysis with chondroitin sulfate ABC enzyme; and (F) purifying the enzyme-inactivated hydrolysate by alcohol precipitation. The product obtained by this method exhibits good acid resistance and is rich in small molecule chondroitin sulfate.

The embodiment of the invention discloses a peptide calcium chelate as well as a preparation method and application thereof. The preparation method of the peptide calcium chelate comprises the following steps: mixing a calcium solution with a wolfberry glycopeptide liquid, adjusting the pH value of the system, carrying out a chelation reaction, cooling with ice water, adding ethanol, centrifuging, and freeze-drying the obtained precipitate to obtain the peptide calcium chelate. The peptide calcium chelate provided by the invention has remarkable calcium supplement advantage and biological activity, calcium extracted from bovine bones and wolfberry peptides are chelated to form a stable compound, the stability of calcium is remarkably improved, the peptide calcium chelate is more convenient to carry and take, and the solubility and absorptivity of calcium ions are increased. Meanwhile, limitation of traditional calcium ion absorption can be avoided through an intestinal peptide transfer channel (such as PepT1), and interference of antagonists in diet is reduced. Scanning electron microscope results prove that the fructus lycii peptide calcium is successfully prepared, and animal experiments show that the fructus lycii peptide calcium can effectively improve the serum calcium level, improve the bone microstructure, increase the bone mineral density and the number of bone trabecula and regulate bone metabolism indexes, and shows a calcium supplement effect and a bone protection effect superior to those of a traditional calcium agent (such as calcium gluconate); the calcium supplement is a novel calcium supplement with high bioavailability and a bone health promoting function.

The application provides application of uterine mesenchymal stem cell exosomes in preparation of drugs for preventing and treating osteoporosis, and belongs to the technical field of biological medicine. The uterine mesenchymal stem cell-derived exosomes provided by the application have extremely low immunogenicity and good biological safety, avoid the immune rejection and adverse reactions possibly caused by traditional drugs or cell therapy, can be effectively targeted to the skeletal system, especially the diseased bone marrow microenvironment, and realize precise drug delivery; the exosomes not only can effectively increase bone density and improve bone microstructure, but also can improve the biomechanical strength of the skeleton, significantly reduce the risk of fracture; in addition, the preparation process is mature and stable, and as a natural cell-free therapeutic product, the exosomes avoid the ethical and safety concerns related to live cell transplantation, and provide a new strategy with great clinical application potential for the prevention and treatment of osteoporosis.

The application belongs to the technical field of health food, and provides a composition for improving osteoarthritis and increasing bone density and application thereof.The composition comprises, in terms of weight fraction, 1-600 parts of eggshell membrane, 1-80 parts of non-denatured type II collagen, 1-900 parts of mangosteen fruit powder and 1-1000 parts of sophora fruit powder.The composition of the application has reasonable compatibility, and each component has clear efficacy, and has remarkable effect in improving bone and joint damage, such as improving osteoarthritis and increasing bone density.

The present invention relates to a novel lappaconitine derivative and a pharmaceutically acceptable salt thereof, a preparation method therefor, and a medical use thereof using osteogenesis-promoting activity. The lappaconitine derivative induces the differentiation of stem cells into preosteoblasts, and increases bone density when administered to an osteoporosis animal model, and induces osteogenesis, and thus can be effectively used for preventing, alleviating, or treating bone-related diseases such as osteoporosis.

The invention provides corn germ bone collagen powder and application thereof in improving osteoarthritis and bone mineral density, and the corn germ bone collagen powder comprises the following components in parts by mass: collagen peptide, corn germ concentrated powder, calcium lactate, eucommia ulmoides leaf extract, coix seed extract, licorice extract, sodium hyaluronate, N-acetylglucosamine and turmeric. The corn germ concentrated powder is added, so that the effects of collagen peptide on promoting hard bone skeletal development, protecting cartilage injury and increasing bone mineral density are greatly improved, particularly, the cartilage injury is protected, and human body effectiveness evaluation tests show that the addition of the corn germ concentrated powder can reduce the pain of arthritis patients, so that the economic benefit of the arthritis patients is improved. And the joint body function of the subject is improved. The technical problem that existing ossein does not give full play to the efficacy of osteoarthritis improvement is solved, and osteoarthritis and bone mineral density are improved at the same time.