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35 results about "Physical trauma" patented technology

Sex reversal siRNA of Chinese mitten crab and application of siRNA

The invention relates to a sex development regulation and control technology for the male Chinese mitten crab, in particular to a sex reversal siRNA of the Chinese mitten crab and application of the siRNA. A nucleotide sequence of a positive-sense strand of the sex reversal siRNA of the male Chinese mitten crab is shown in SEQ ID NO.1, and a nucleotide sequence of an antisense strand is shown in SEQ ID NO.2. according to the sex reversal siRNA, an IAG gene segment of the Chinese mitten crab is screened from an androgenic gland transcriptome of the Chinese mitten crab, the total-length cDNA sequence of the segment is cloned, according to an IAG gene sequence of the Chinese mitten crab, the siRNA is designed, synthesized and guided into a body of the Chinese mitten crab in the sex differentiation period, expression of an IAG gene in an androgenic gland cell is disturbed, and sex reversal of the male crab is achieved. Compared with androgenic gland removal through surgery, a micro-injection method is adopted and has the advantages that the trauma to the body of the Chinese mitten crab is small, and the situation is avoided that due to wound infection after surgery, the Chinese mittencrab dies; the injection dose is extremely low, the immunological stress reaction of a tested animal is not easily triggered, and accordingly the success rate of sex reversal is increased.
Owner:WEIFANG UNIV OF SCI & TECH

Paste for treating physical trauma

The invention discloses a paste for treating physical trauma in the field of Chinese medicaments, which is prepared by using 5 portions of borneol, 150 portions of cochinchina momordica seed, 10 portions of calomel, 500 portions of valelinum Liquidum, 70 portions of fraxinus chinensis, 5 portions of minium and 200 portions of honey as raw materials. The paste has effects of clearing away heat and toxic material, sterilizing and stanching bleeding, relieving swelling and pains, drawing out the pus and promoting tissue regeneration and accelerating wound healing, wherein the effect of relieving pains is particularly outstanding. The paste is also characterized by forming a protective film on the surface of a body to isolate bacteria and viruses and to prevent the bacteria and the viruses from entering the body from wounds so as to prevent and control infection effectively to cure the physical trauma quickly. The paste also has the characteristics of novel and unique formula, reasonable drug mixing, fewer and readily-available components, exact and long-lasting drug effect, short treating period, quick response, remarkable treatment effect, radical prevention of relapse after treatment, no toxic and side effect, and suitability for clinic promotion. The paste has total effective rate of 100 percent and a cure rate of 100 percent for treating the physical trauma, physical traumatic infections, high fever and edema, local tissue necrosis and complications and is the most ideal drug for treating the physical trauma currently.
Owner:葛锐民

A kind of Lycium barbarum aseptic seedling and method for obtaining callus thereof

InactiveCN103609451BShort cycleOvercoming seasonal selectivity in callusHorticulture methodsPlant tissue cultureEvery Two WeeksPolyethylene glycol
The invention discloses a medlar sterile seedling and a method for obtaining the induced healing of the medlar sterile seedling, belonging to the technical field of plant tissue culture. The method comprises the following steps of drying fruits; disinfecting seeds: carrying out the seed disinfection by using sterile distilled water which contains 70 percent by weight of ethanol and 0.1 percent by weight of TritonX-100 (polyethylene glycol octylphenol ether); germinating seeds: firstly arranging the seeds into a refrigerator at 4 DEG C, standing for 2 days, and then removing the seeds to a culture room at the temperature of 25+ / -2 DEG C with the daily photoperiod of 14 hours to culture for 20+ / -2 days; healing subculture: arranging the seed on a subculture medium, culturing under at 25+ / -2 DEG C in the culture room with the daily photoperiod of 16 hours, and replacing the culture medium every two weeks. According to the method disclosed by the invention, both the medlar sterile seedling and the medlar induced callus are obtained, the step of carrying out physical trauma on the medlar callus in the inducing process of the medlar callus is simplified and the period of obtaining the medlar callus is shortened.
Owner:SHANDONG CROP GERMPLASM CENT
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