The invention discloses a spray formula for the muscle injury and a manufacturing process of the spray formula, relates to the field of medical and health services, and particularly relates to a medicinal spray formula for treating the symptoms of muscle fatigue, bruise and strain and the manufacturing process of the spray formula. The medicinal spray formula for treating the muscle injury comprises the following raw medicinal materials of safflower carthamus, a cyperus rotundus powder, radix cyathulae, ligusticum wallichii, anise, radix angelicae, myrrh, moutan bark, borneol, radix clematidis, divaricate saposhnikovia root, and mint oil; the manufacturing process comprises the following steps: 1, drying in the sum; 2, stirring and screening the materials (not including mint oil) by a 30-mesh sieve; 3, soaking and fermenting; 4, filtering a dreg by using a filter screen, taking a medicinal liquid of the filtered medicines; 5, mixing the mint oil into the medicinal liquid, stirring, sealing, and standing for 1 hour; and 6, bottling. The spray formula is an external-use spray prepared from the pure traditional Chinese medicines, reaches the focus of infection through the outer skin,and achieves a better treatment effect; the pain is alleviated after the spray formula is used for 5-10 minutes, and the patient can normally move and work after one day.