18 results about "Bactrocera" patented technology

The invention relates to bacillus thuringiensis and application of the bacillus thuringiensis in luring adult fruit flies, the classification name of the bacillus thuringiensis is bacillus thuringiensis XLB2910, the bacillus thuringiensis is preserved in the China Center for Type Culture Collection on May 19, 2025, and the strain preservation number is CCTCC NO: M 20251097. The strain disclosed by the invention is subjected to shake culture and fermentation in a GYS culture medium, and the supernatant of the fermentation liquor shows efficient attraction activity on female and male adults of bactrocera cucurbitae and bactrocera minax, so that the strain has relatively high practical application value and can be used for preventing and treating bactrocera minax.

The invention discloses a sex attractant for bactrocera dorsalis and application of the sex attractant. The invention provides a sex attractant for bactrocera dorsalis. The sex attractant for bactrocera dorsalis comprises ethyl laurate, ethyl myristate, cis-9-hexadecenoic acid ethyl ester and ethyl palmitate. The sex attractant provided by the invention can effectively reduce the population density of male adult bactrocera dorsalis in the field, reduce successful mating of female adult bactrocera dorsalis, and reduce the reproduction rate of bactrocera dorsalis. The sex attractant developed by the invention is a novel bactrocera dorsalis attractant, can enrich compound resources for biological prevention and control of bactrocera pests and promote the development of a pest comprehensive prevention and control strategy towards the directions of high efficiency, economy, greenness and the like, and has very high application value.

The invention provides a composition for luring bactrocera cucurbitae and application thereof, and relates to the technical field of green prevention and control of agricultural pests. The composition comprises the following active ingredients in percentage by weight: 10% of muscovone, 0.1% of heptanal and 0.1% of hexene. The main components of the composition all come from natural plant volatile matters, and the composition is easy to degrade in the natural environment, has no residual risk and is friendly to the ecological environment. Meanwhile, the composition has high safety to bees, pollination insects and other non-target organisms, and ecological side effects possibly brought by traditional chemical attractants are effectively avoided. According to the implementation of the method, an efficient and specific tool is provided for field monitoring of bactrocera cucurbitae populations, the occurrence dynamic of the bactrocera cucurbitae can be accurately mastered, the early warning capability is improved, a new technical path and an implementation basis are provided for promoting green comprehensive treatment of the bactrocera cucurbitae, and the method has good popularization and application prospects.

The invention relates to the technical field of comprehensive treatment of agricultural pests, and particularly provides a bactrocera cucurbitae double-trapping type attractant based on optimal matching of host and intestinal bacteria volatile matters and application of the bactrocera cucurbitae double-trapping type attractant. The attractant comprises an active component and a synergistic component, the active component is derived from balsam pear and towel gourd volatile matters and bactrocera cucurbitae intestinal bacterial metabolites, and comprises 2-methyl ethyl valerate, propyl hexanoate, ethyl hexadienoate, heptanal, benzyl alcohol, 3-methyl acetic acid-1-butanol, indole and 1-undecanol; and the synergistic component is myzobenzoic ketone. Active components are quickly released in the early stage of application, so that the attraction efficiency is remarkably improved; the synergistic component prolongs the trapping lasting period through the slow release effect, and the long-term trapping effect is ensured. All the components of the attractant provided by the invention are biological sources or environment-friendly chemical substances, the attractant meets the requirement of green prevention and control, can efficiently attract male and female adults at the same time, makes up the limitation that a traditional sex attractant only induces male adults or a food attractant only induces female adults, and is beneficial to more comprehensive control of population quantity.

The invention discloses Lap3a, Lap3b and Lap3c genes of bactrocera cucurbitae and an application of the Lap3a, Lap3b and Lap3c genes. The space-time and tissue expression profiles of the Lap3a, Lap3b and Lap3c genes of the bactrocera cucurbitae show that the three genes are specifically and highly expressed in testis of male bactrocera cucurbitae, especially in transformation regions in testis tissues of adult bactrocera cucurbitae. The Lap3a, Lap3b and Lap3c genes play important functions in the process of participating in maintaining the male reproduction of the bactrocera cucurbitae, when the Lap3a, Lap3b or Lap3c genes of male bactrocera cucurbitae are inhibited, the number of male sperms of the bactrocera cucurbitae is reduced, the reproductive power of the male bactrocera cucurbitae is reduced, the treated male bactrocera cucurbitae is mated with normal female bactrocera cucurbitae, and the egg laying amount and egg hatching rate of the female bactrocera cucurbitae are reduced. The three genes are expected to become SIT potential targets to be applied to pest control, and a new thought is provided for green control of bactrocera cucurbitae.

The invention discloses an application of a CTL-S1 gene and miR-let-7 in regulation and control of reproductive development of bactrocera cucurbitae. The CTL-S1 gene has high specificity expression in bactrocera cucurbitae ovary, and the hatching rate of offspring can be reduced by inhibiting the expression of the CTL-S1 gene through RNA interference. Through the analysis of a dual luciferase report system of RNA co-immunoprecipitation, the result shows that the miR-let-7 and the CTL-S1 have a targeting relationship. Overexpression of miR-let-7 can lead to significant reduction of the expression quantity of the CTL-S1 gene, and a phenotype similar to RNA interference inhibition of the expression of the CTL-S1 gene appears, so that the hatching rate of offspring is reduced. The invention provides a potential gene target for developing a genetic control strategy for destroying the reproduction of bactrocera cucurbitae, provides a new thought for the subsequent biological control of insects by adopting a sterile technology, and has a wide application prospect.

The application discloses CTL-S1 A gene and an application of miR-let-7 in regulation of reproductive development of drosophila bacterea CTL-S1 The gene is specifically highly expressed in ovaries of drosophila bacterea, and the expression of the gene is inhibited by RNA interference CTL-S1 The expression of the gene can reduce the hatching rate of offspring. Through analysis of an RNA immunoprecipitation double luciferase reporter system, it is found that miR-let-7 and CTL-S1 there is a target relationship. Overexpression of miR-let-7 can cause CTL-S1 the expression amount of the gene to be significantly reduced, and a phenotype similar to the expression of the gene being inhibited by RNA interference CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S is generated, and the hatching rate of offspring is reduced. The application provides a potential gene target for developing a genetic control strategy for destroying the reproduction of drosophila bacterea, provides a new idea for subsequent biological control of insects by using the sterile technique, and has a wide application prospect.

The utility model relates to the technical field of trapping and killing of bactrocera dorsalis, in particular to a bactrocera dorsalis trap. Comprising a base table, the base table is of a circular plate structure, an annular base is fixedly arranged at the top end of the base table, an inner annular groove and an outer annular groove are formed in the upper portion of the annular base, the annular base is fixedly connected with an inner-layer separation net and an outer-layer shell through the inner annular groove and the outer annular groove respectively, and a supporting base is fixedly arranged on one side of the annular base; two springs are fixedly connected to one side of the supporting seat, a movable plate is fixedly connected to one side of each spring and is of a transverse T-shaped platy structure, the tail end of each movable plate is fixedly connected with one side of the outer-layer shell in an inserted mode, and a cylindrical insect trapping bottle is arranged on the center side of the top end of the annular seat. The utility model aims to solve the technical problems that in the prior art, pesticides are directly sprayed on fruits or fruit trees to prevent and control citrus fruit flies, but pesticide residues are easily generated on the fruits due to multi-frequency spraying, and the ecological environment is threatened.

The invention relates to the technical field of prevention and control of bactrocera cucurbitae, in particular to high-temperature-resistant beauveria bassiana WZS-5 and application thereof to prevention and control of bactrocera cucurbitae, and the preservation number of the high-temperature-resistant beauveria bassiana WZS-5 is GDMCCNO: 66358. The optimal growth temperature of the strain is 28-30 DEG C, and the strain still keeps certain growth and reproduction capability at the high temperature of 35 DEG C. Biological assay results show that under the conditions that the temperature is 30 DEG C and the relative humidity is 70%, the prevention and treatment effect of the strain on beauveria bassiana is remarkably better than that of common beauveria bassiana, and the accumulative death rate of bactrocera cucurbitae adults under the concentration of 1 * 10 < 9 > spores / mL reaches 89%. Results show that the strain has excellent high-temperature adaptability and strong pathogenic activity, can efficiently control bactrocera cucurbitae populations in tropical and subtropical high-temperature environments, and provides new microbial resources and technical approaches for green prevention and control of fruit and vegetable pests.

The present application belongs to the technical field of pesticides, and particularly relates to a kind of insecticidal composition for preventing and treating Dacus oleae. An insecticidal composition for preventing and treating Dacus oleae, wherein the insecticidal composition is prepared by compounding doramectin with spirodurable or flubrocythrinate; the mass ratio of doramectin to spirodurable is 1-15:30-1; the mass ratio of doramectin to flubrocythrinate is 1-20:5-1. When doramectin is compounded with spirodurable or flubrocythrinate, the compound combination shows a joint synergistic effect on Dacus oleae within the mass ratio range of 1-15:30-1 and 1-20:5-1, which indicates that the insecticidal composition of the present application can improve the prevention and treatment effect on Dacus oleae.

The utility model provides a kind of convenient egg taking device of real fly, comprising: attractant containing cup and oviposition needle insertion cup, attractant containing cup and oviposition needle insertion cup are connected by thread, the oviposition needle insertion cup is installed in the upper of attractant containing cup, the oviposition needle insertion cup surface is evenly provided with needle hole with 0.1-1mm diameter. The device is simple in structure, reasonable in design, easy to assemble and use, can quickly and conveniently collect the egg particles of Bactrocera dorsalis and Bactrocera cucurbitae, and the size of oviposition hole limits the oviposition of fruit fly, avoids the phenomenon that different kinds of fly larvae will appear when collecting larvae in rotten melon and rotten fruit in field, and the application scenario is wide.

The invention discloses a fruit fly attractant and a preparation method thereof, and the preparation method comprises the following steps: step S1, purchasing fruit raw materials from a fruit market and an orchard, washing and peeling the fruit raw materials, and then grinding the fruit raw materials into slurry by a pulping machine; s2, adding the obtained slurry into a fermentation barrel, and adding sporospora globosa into the fermentation barrel for sealed fermentation; s3, after fermentation is finished, adding an auxiliary material additive into the fermentation barrel, and uniformly stirring and mixing to obtain a mixture; s4, detecting the pH value, the sugar content and the volatile organic compounds of the obtained mixture, and continuing fermentation if the detection indexes are unqualified; and if the detection indexes are qualified, obtaining the product, namely the fruit fly attractant, and packaging the product by adopting a full-automatic packaging machine. Compared with a traditional pesticide, the fruit fly attractant does not have the problems of pesticide residues or damage to land and environment and the like, and has the advantages of being environmentally friendly, free of pollution and the like.

The present application relates to the technical field of molecular biology, in particular to a Bactrocera cucurbitae Hsc70_1 and Hsc70_2 gene and application thereof.The technical problem to be solved by the present application is to provide a new option for green and sustainable control of pest sterilization technology.The technical scheme of the present application is a Bactrocera cucurbitae Hsc70_1 gene and / or Hsc70_2 gene, the nucleotide sequence of the Hsc70_1 gene is shown as SEQ ID No.1, and the nucleotide sequence of the Hsc70_2 gene is shown as SEQ ID No.2.By reducing the expression of the two genes, the fertility of Bactrocera cucurbitae male insects can be reduced, and potential targets are provided for green and sustainable control of pest sterilization technology.

The present invention discloses a formulation comprising phagostimulants or kairomones, stabilizer, emulsifying, thickening and temperature stabilizing agents to control Tephritid fruit fly (Bactrocera cucurbitae) by attracting both male and female of all stages thereby said formulation finds a prominent place in integrated pest management, control and eradication programs of tephritid fruit flies. The process of preparation of the formulation is also disclosed in the present invention.

The invention relates to the field of pesticide preparations, and discloses a bactrocera cucurbitae and South Asia fruit fly repellent and application thereof.The repellent is a preparation with phenylacetaldehyde as an effective component, a phenylacetaldehyde solvent in the repellent is ultrapure water or dimethyl sulfoxide, and the content of phenylacetaldehyde in the repellent is 0.005-0.2% by weight. The phenylacetaldehyde repellent can significantly reduce the oviposition behaviors of the two fruit flies on host plants, so that fruits are effectively protected from being invaded by pests; in addition, the repellent disclosed by the invention can also play a role in preventing and controlling various fruit fly pests at the same time, and has important significance on comprehensive management of pests of various crops. Therefore, when the phenylacetaldehyde is used for repelling pests, the use amount of chemical pesticides can be reduced, the pollution to the ecological environment is reduced, the requirements of modern green agriculture and sustainable development are met, and the phenylacetaldehyde has a good application value in pest repelling, preventing and controlling of bactrocera cucurbitae or South Asia fruit flies.

The invention relates to the technical field of green prevention and control of agricultural pests, in particular to a female bactrocera cucurbitae attractant based on volatile matters of bitter gourd fruits and leaves and application of the female bactrocera cucurbitae attractant. The active components of the female bactrocera cucurbitae attractant come from volatile matters of fruits and leaves of bitter gourds, and comprise 4-terpilenol, verbenol, alpha-terpilenol, capraldehyde, beta-caryophyllene and perillaldehyde. The main components of the female bactrocera cucurbitae attractant are all derived from natural plant volatile matters, the attractant is environment-friendly, a foundation is laid for field monitoring of bactrocera cucurbitae, and a new thought is provided for green prevention and control of bactrocera cucurbitae.

The application discloses a kind of bactrocera cucurbitae larvae feeding preference and host selection determination device and use method, it is related to insect feeding preference and host selection determination technical field, including box and box cover, box cover is set in the upper end of box, the bottom of box is set with the drop port in the center, the lower end of drop port is set with threaded tube, threaded tube is connected with drop pipe, fixed device is set on box, fixed device includes the fixed nail being set above box and the fixed magnet being set below box, the upper end of fixed magnet is set with positioning card, the center of positioning card is set with positioning hole, positioning hole is connected with threaded tube, the lower end of positioning card is set with card fixed plate, the center of card fixed plate is set with fixed hole, fixed hole is connected with threaded tube, card fixed plate is set in the upper end of fixed magnet.The application uses the above-mentioned structure of a kind of bactrocera cucurbitae larvae feeding preference and host selection determination device and use method, with the advantages of simple structure, easy to operate.

The invention relates to the technical field of agricultural disaster monitoring, in particular to an early recognition method for bactrocera hippophae pests based on unmanned aerial vehicle multispectral imaging. The method comprises the following steps: carrying out flight data acquisition in a sea-buckthorn key phenological period through an unmanned aerial vehicle multispectral imaging system; carrying out preprocessing and radiation correction on the image data; extracting a hippophae rhamnoides canopy region and calculating spectral characteristics of the An improved deep convolutional neural network model is constructed and trained, the model integrates a multi-scale feature fusion structure and a channel attention mechanism, and a spatial pattern and internal association of spectral features can be deeply analyzed; and finally, the model is utilized to realize early recognition and positioning of the bactrocera hippophae pests. According to the invention, early weak spectral response changes caused by insect pests can be effectively captured, and the automation degree, timeliness and accuracy of identification operation are improved.