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Bactrocera is a large genus of tephritid fruit flies, with close to 500 species currently described and accepted.
Application of CTL-S1 gene and miR-let-7 in regulating the reproductive development of Bactrocera cucurbitae
ActiveCN121496008Blower hatch rateOther foreign material introduction processesFermentationAnimal scienceGene targeting
The application discloses CTL-S1 A gene and an application of miR-let-7 in regulation of reproductive development of drosophila bacterea CTL-S1 The gene is specifically highly expressed in ovaries of drosophila bacterea, and the expression of the gene is inhibited by RNA interference CTL-S1 The expression of the gene can reduce the hatching rate of offspring. Through analysis of an RNA immunoprecipitation double luciferase reporter system, it is found that miR-let-7 and CTL-S1 there is a target relationship. Overexpression of miR-let-7 can cause CTL-S1 the expression amount of the gene to be significantly reduced, and a phenotype similar to the expression of the gene being inhibited by RNA interference CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S is generated, and the hatching rate of offspring is reduced. The application provides a potential gene target for developing a genetic control strategy for destroying the reproduction of drosophila bacterea, provides a new idea for subsequent biological control of insects by using the sterile technique, and has a wide application prospect.
Owner:FUJIAN AGRI & FORESTRY UNIV