28 results about "Perillaldehyde" patented technology

The present invention discloses a method for producing perillaldehyde and / or perillyl lactone, comprising: contacting a first component with a second component to produce perillaldehyde and / or perillyl lactone; wherein the first component is selected from components containing lysine-2-diol, and the second component is selected from enzymes or microorganisms or their cell lysates or enzyme extracts capable of converting lysine-2-diol into perillaldehyde and / or perillyl lactone.

The application discloses an antifungal effect of an antifungal emulsion and application of the antifungal emulsion in prevention and treatment of chestnut corruption, and belongs to the technical field of food preservation. The application is used for preventing and treating corruption caused by fungal infection during the collection and storage period of chestnuts. The antifungal emulsion comprises an antifungal component, an emulsifier and distilled water. The antifungal component comprises at least one of plant essential oil and active components of plant essential oil. The plant essential oil is at least one of thyme essential oil, clove essential oil and perilla essential oil. The active components of plant essential oil are at least one of thymol, eugenol and perillaldehyde. The application has good prevention and treatment effect on fungal infection of chestnuts during the collection and storage period, and has the advantages of being green, safe and efficient.

The invention discloses edible fresh-keeping gel beads for a vegetable protein beverage and a preparation method of the edible fresh-keeping gel beads, and belongs to the technical field of functional food and intelligent packaging. According to the edible fresh-keeping gel beads prepared by the preparation method disclosed by the invention, Schiff base formed by perillaldehyde-yeast protein is used as a liquid core, and Ca < 2 + > is used for pre-crosslinking; the wall material is a sodium alginate-rice protein composite network and can intelligently respond to imine bond protonation caused by pH drop in the beverage storage process, so that controllable release of perillaldehyde is realized, protein aggregation and microbial growth are inhibited, and a clean label and original sense are kept. According to the invention, triple advantages of edible, intelligent response and low cost are taken into consideration, and a plug-and-play fresh-keeping solution is provided for the plant-based protein beverage.

This invention belongs to the field of biosynthesis technology, specifically relating to a strain for producing perillaldehyde, its construction method, and its application. Addressing the microbial synthesis problem of perillaldehyde, this invention mutates and optimizes the phosphotransketase in the NOG pathway, using a strain derived from nanoarchaemonas (…). Nanoarchaeota archaeon Site-directed mutagenesis was performed on the phosphoketolase NaXpk, and a perillaldehyde synthesis pathway was constructed in E. coli. The effects of NaXpk mutation on perillaldehyde yield were compared to identify the S472F and S472Y mutants. These mutants were then applied to the perillaldehyde production pathway, and the resulting production strains achieved perillaldehyde yields of 623.3 mg / L and 655.2 mg / L, respectively, demonstrating significant effectiveness.

The application discloses a kind of medicine food homologous sputum-removing and spleen-strengthening compositions and preparation method and application thereof, the composition includes: naringin 120-200 parts, hesperidin 80-100 parts, tuckahoe polysaccharide 150-250 parts, coix seed polysaccharide 80-120 parts, gingerol 10-20 parts, perillaldehyde 5-18 parts, glycyrrhizic acid 10-25 parts and glycyrrhizin flavone 8-24 parts by weight parts.The composition of the application can be prepared into medicine food homologous product, and it is not against disease, but against symptom, and has significant, stable and safe improvement effect on phlegm, throat discomfort, abdominal distension, thick and greasy tongue fur and other phlegm-related symptoms, without obvious toxic and side effects, and meets daily consumption requirements.

This invention relates to the field of agricultural product storage, and more particularly to a fumigation method for preventing soft rot in agricultural products. The method involves fumigation using a cellulose-based material as a carrier, perillaldehyde and / or salicylaldehyde as a base, and the addition of isobutyric acid and / or 2-methylbutyric acid. This invention effectively inhibits and kills *Rhizopus spp.*, the pathogen causing soft rot in agricultural products, maintaining the physiological quality and nutritional value of agricultural products during storage, and significantly extending the storage period. This invention is highly effective, simple to operate, environmentally friendly and safe, and easily scaled up and industrialized.

The invention belongs to the technical field of insect pest prevention and control, and particularly relates to application of a plant volatile matter to prevention and control of flower thrips, and the plant volatile matter comprises perillaldehyde and / or beta-pinene. Perillaldehyde and / or beta-pinene are / is separated from an aromatic plant perilla frutescens, has a remarkable repelling effect on the flower thrips, has a function of inducing natural enemies of the perilla frutescens, can effectively prevent and control the flower thrips, provides a scientific basis for a green prevention and control strategy of the flower thrips, development of plant-derived repellents and application of an ecological regulation and control technology, and has a wide application prospect. And the method has a relatively high application value.

This invention belongs to the field of biosynthesis, specifically relating to a variant of lysine pyrophosphate diol synthase and the constructed engineered yeast strain for perillaldehyde synthesis. To address the generally low yield of perillaldehyde biosynthesis in existing technologies, this invention mutates and optimizes the lysine pyrophosphate diol synthase to obtain an SsLPPs mutant containing the G379M mutation, which is then used for perillaldehyde synthesis. Simultaneously, to address the genetic instability issues associated with free plasmid expression in some Saccharomyces cerevisiae strains, this invention constructs a perillaldehyde synthesis pathway within the yeast genome. Furthermore, by enhancing the acetyl-CoA synthesis pathway, the MVA pathway, and the perillaldehyde synthesis pathway, and by knocking out some transcriptional repressors, the substrate strain synthesizing perillaldehyde is modified, resulting in a significantly increased yield compared to existing technologies.

The invention belongs to the field of biosynthesis, and in particular relates to an improved variant of libdanenediol pyrophosphate synthase and engineered yeast for producing sclareol. In order to solve the problem that in the prior art, the sclareol biosynthesis yield is generally low, LsLPPs mutant containing N674L mutation is obtained by performing mutation and optimization on LsLPPs pyrophosphate synthase, and the mutant is used for sclareol synthesis. Meanwhile, in order to solve the problem of genetic instability caused by free plasmid expression of part of saccharomyces cerevisiae strains, a sclareol synthesis pathway is constructed in a yeast genome, and meanwhile, an acetyl coenzyme A synthesis pathway, an MVA pathway and a sclareol synthesis pathway are enhanced; and the chassis bacteria for synthesizing sclareol are modified in a manner of knocking out part of transcription inhibition factors, so that the yield is greatly improved compared with the prior art.

The invention belongs to the field of food additives, and particularly discloses a blended houttuynia cordata flavor essence and a preparation method thereof.The blended houttuynia cordata flavor essence comprises, by mass, 3%-6% of 2-decenal, 2%-5% of 2-nonenal, 2%-6% of 2, 4-dihydroxy-2-methyl-1, 3-pentanedione, 1%-3% of 2, 4-dihydroxy-2, 4-pentanedione, 1%-3% of The formula comprises the following components in percentage by weight: 1%-3% of 1, 4-decadienal, 3%-5% of 2-decanone, 1%-3% of lauric aldehyde, 1%-2% of myrcene, 4%-7% of beta-caryophyllene, 0.5%-1.5% of cis-3-hexene-1-ol, 2%-3% of 2-hexenal, 2%-5% of linalool, 0.5%-1.5% of 1-octylene-3-ol, 0.5%-1.5% of perillaldehyde, 2%-3% of 2-amyl furan, 1%-3% of eucalyptus oil, 1%-2% of eugenol and the balance of water. The composition is prepared from 0.5%-1.5% of methyl dihydrojasmonate, 3%-5% of cis-6-nonene-1-ol, 1%-3% of 2-acetylpyridine, 1%-3% of cis-6-nonene-1-ol, 1%-3% of 2-acetylpyridine, 1%-3% of cis-6-nonene-1-ol, 1%-3% of vetiver oil and the balance of caprylic capric triglyceride. The blended houttuynia cordata flavor essence is prepared from the monomer spice for food, and has the characteristics of obvious characteristic fragrance, strong natural feeling, long fragrance retention time and high temperature resistance.

The application discloses a method for recovering perillyl alcohol crude product from perillyl alcohol mother liquor, comprising the following steps: (1) perillyl alcohol in the perillyl alcohol mother liquor is deoximated under the catalysis of acid to generate perillyl aldehyde crude product; (2) perillyl aldehyde is subjected to distillation and impurity removal to obtain perillyl aldehyde with high content; (3) the purified perillyl aldehyde is added into a hydroxylamine solution to perform oximation reaction, and perillyl alcohol crude product can be obtained after the reaction. The method can remove impurities through the distillation method after the perillyl alcohol mother liquor is converted into perillyl aldehyde crude product, and then the oximation is performed again to obtain perillyl alcohol crude product. About 50% of perillyl alcohol in the perillyl alcohol mother liquor can be recovered, and the total yield of perillyl alcohol industrial production is increased.

The application discloses a quercetin-eucalyptus oil composition for clearing and protecting lungs, and relates to the technical field of functional food. The composition comprises an active functional component and a three-phase layered targeting delivery system. The active functional component is composed of quercetin 5-20 parts, eucalyptus oil 1-8 parts, bromelain 2-10 parts, dihydromyricetin 10-30 parts, perillaldehyde 1-6 parts, icariin 3-15 parts and ultralow-molecular-weight sodium hyaluronate 1-5 parts in terms of weight fraction. The three-phase layered targeting delivery system comprises three independent embedding units, namely enteric-soluble protective microspheres for wrapping bromelain, nano-mixed micelles for wrapping quercetin, dihydromyricetin and icariin, and slow-release microcapsules for wrapping eucalyptus oil and perillaldehyde. The application solves the problems of component compatibility conflict and component inactivation in traditional compositions by scientifically matching seven active functional components and combining the three-phase layered targeting delivery system, improves the utilization rate of components, can be produced on a large scale, and improves the storage stability of the composition.

This invention provides a recombinant lipolytic yeast strain with high perillaldehyde production, its preparation method, and its applications. This recombinant lipolytic yeast strain can produce perillaldehyde with high yield and efficiency, effectively solving the problem of low yield in existing recombinant Yersinia lipolytic yeast fermentation for perillaldehyde production. Furthermore, the batch-to-batch stability of perillaldehyde production using this recombinant lipolytic yeast strain is good, meeting the requirements for product stability in large-scale industrial production. Therefore, the recombinant lipolytic yeast strain of this application has high production efficiency in perillaldehyde production, thus showing good prospects for industrial application.

This invention provides an engineered microorganism and a method for synthesizing perillaldehyde. The engineered microorganism comprises a gene for perillaldehyde synthase and a gene for Bayer-Villiger monooxygenase. The perillaldehyde synthase comprises: (a1) a protein having any sequence in SEQ ID NOs: 1-25, or; (a2) a protein having more than 70% homology with the amino acid sequence defined in (a1) and possessing perillaldehyde synthase activity. The Bayer-Villiger monooxygenase comprises: (b1) a protein having any sequence in SEQ ID NOs: 26-50, or; (b2) a protein having more than 70% homology with the amino acid sequence defined in (b1) and possessing Bayer-Villiger monooxygenase activity. This invention solves the problem of inefficient biosynthesis of perillaldehyde in existing technologies and is applicable to the field of synthetic biology.

The invention relates to the technical field of microorganisms, in particular to a method for improving quality and insect resistance of purple perilla through inoculation of arbuscular mycorrhizal fungi, which is characterized in that the arbuscular mycorrhizal fungi are rhizosporium endophyllum, and insects are beet armyworms. The arbuscular mycorrhizal fungi are inoculated, so that the growth of the aboveground part and the underground part of the purple perilla is remarkably promoted, and the biomass of the purple perilla is improved; accumulation of linalool, perillyl alcohol, perillaldehyde, elemene, ocimene and limonene in the perilla leaves can be promoted, and the quality of the perilla is improved; the content of defense secondary metabolites such as flavone and tannin of the perilla leaf and the activity of defense enzymes such as polyphenol oxidase and phenylalanine ammonialyase can be improved, and the resistance of the perilla leaf to beet armyworms is improved. After the arbuscular mycorrhizal fungi inoculated perilla leaves are eaten, the growth and development of the beet armyworms are inhibited, and the beet armyworms show that the weight and the body length are reduced, and the developmental duration is prolonged.

This invention relates to the field of tobacco technology, specifically to a flavored tobacco extract, its preparation method, and its application. The flavored tobacco extract provided by this invention comprises: ceftazidimetrienol, megastigmatrienone, nicotine, β-ionone, 4-hydroxy-β-dihydrodamascone, isovaleric acid, 2-methylbutyric acid, and perillaldehyde; the mass ratio of ceftazidimetrienol, megastigmatrienone, nicotine, β-ionone, 4-hydroxy-β-dihydrodamascone, isovaleric acid, 2-methylbutyric acid, and perillaldehyde in the flavored tobacco extract is (1.5-3.3):(0.1-0.25):(3.5-15):(0.0015-0.01):(0.01-0.1):(0.05-0.5):(0.005-0.02):(0.05-0.4). The aromatic tobacco extract provided by this invention has high safety, while the extract has a distinct aroma, good overall harmony, and a good overall sensory experience.

The present invention discloses a quercetin-eucalyptus-oil composition for clearing and protecting the lungs, relating to the technical field of functional foods. The composition comprises active functional components and a three-phase layered targeted-delivery system. The active functional components comprise, in parts by weight, 5-20 parts quercetin, 1-8 parts eucalyptus oil, 2-10 parts bromelain, 10-30 parts dihydromyricetin, 1-6 parts perillaldehyde, 3-15 parts icariin, and 1-5 parts ultra-low-molecular-weight sodium hyaluronate. The three-phase layered targeted-delivery system comprises three independent encapsulation units, namely enteric-protective microspheres encapsulating bromelain, nano mixed micelles encapsulating quercetin, dihydromyricetin, and icariin, and sustained-release microcapsules encapsulating eucalyptus oil and perillaldehyde. By scientifically combining the seven active functional components with the three-phase layered targeted-delivery system and by improving the preparation process, the present invention solves the problems of component incompatibility and easy inactivation in conventional compositions, improves ingredient utilization, enables large-scale production, and enhances the storage stability of the composition.

The invention discloses a perillaldehyde derivative as well as a preparation method and application thereof. The structural formula of the perillaldehyde derivative is shown in the specification. The compound disclosed by the invention is simple in structure and few in synthesis steps; the compound has an obvious inhibition effect on physalospora piricola, valsa mali, phytophthora capsici, rhizoctonia solani and gaeumannomyces graminis, and can be used as a bactericide for preventing and treating plant diseases.

This invention belongs to the field of biosynthesis, specifically relating to a variant of lysine pyrophosphate diol ester synthase and engineered bacteria for producing perillaldehyde. To address the generally low yield of perillaldehyde biosynthesis in existing technologies, this invention mutates and optimizes lysine pyrophosphate diol ester synthase, obtaining an SsLPPs mutant containing the D374L mutation, and uses this mutant for perillaldehyde synthesis. Simultaneously, to address the genetic instability issues associated with free plasmid expression in some Saccharomyces cerevisiae strains, this invention constructs a perillaldehyde synthesis pathway in the yeast genome. Furthermore, it modifies the substrate bacteria synthesizing perillaldehyde by enhancing the acetyl-CoA synthesis pathway, the MVA pathway, and the perillaldehyde synthesis pathway, and by knocking out some transcriptional repressors, resulting in a significant increase in yield compared to existing technologies.

The present application belongs to the field of bioengineering, and relates to an engineering strain, in particular to a pantoea that degrades vetiverol and application. The preservation number of the strain is CGMCC NO.30671. The pantoea that degrades vetiverol in the present application can convert vetiverol, an intermediate product in ambergris, into a friendly ambergris substance, and the degradation rate of vetiverol is above 65.99% within 72 h. The pantoea is used for fermentation of tobacco leaves, which is helpful to improve the quality of tobacco leaves and improve the sensory quality of cigarettes.

This invention discloses a perillaldehyde synthase mutant, a recombinant genetically engineered strain, and their applications, belonging to the field of biotechnology. This invention modifies perillaldehyde synthase through semi-rational design, resulting in a perillaldehyde synthase mutant with good catalytic activity, significantly increasing perillaldehyde yield; furthermore, by integrating the encoding of HMG-CoA reductase... HMG1 Gene, encoding isopentenyl pyrophosphate isomerase IDI1 Gene encoding geraniol geraniol pyrophosphate synthase SsGGPPS Gene, encoding lysine pyrophosphate diol ester synthase SsLPPS The recombinant genetically engineered strain obtained from the gene and nucleic acid molecule encoding the perillol synthase mutant can produce high yields of perillol using glucose as a substrate, and has significant industrial application value.

The invention relates to the technical field of green prevention and control of agricultural pests, in particular to a female bactrocera cucurbitae attractant based on volatile matters of bitter gourd fruits and leaves and application of the female bactrocera cucurbitae attractant. The active components of the female bactrocera cucurbitae attractant come from volatile matters of fruits and leaves of bitter gourds, and comprise 4-terpilenol, verbenol, alpha-terpilenol, capraldehyde, beta-caryophyllene and perillaldehyde. The main components of the female bactrocera cucurbitae attractant are all derived from natural plant volatile matters, the attractant is environment-friendly, a foundation is laid for field monitoring of bactrocera cucurbitae, and a new thought is provided for green prevention and control of bactrocera cucurbitae.

This invention discloses a humidity- and pH-responsive aerogel preservation pad, its preparation method, and its application. The preparation method of the aerogel preservation pad includes the following steps: mixing bacterial cellulose and cellulose nanofibers, and then adding a hydrothermal polymer of perillaldehyde-carboxymethyl chitosan to obtain a mixed solution; performing a freeze-freezing treatment according to freezing parameters to form an ice template and then performing an immersion treatment; and finally drying the ice template according to atmospheric pressure drying parameters to obtain the aerogel preservation pad. The aerogel preservation pad prepared by this method releases perillaldehyde as the ambient humidity increases and the pH value decreases, thus being applied to the preservation of fresh fruits and vegetables, extending the shelf life of fruits and vegetables, and solving the problems of poor preservation effect and difficult degradation of preservation pads in existing room temperature preservation technologies.

This invention relates to a dual-membrane separation process for extracting the oil phase from perillaldehyde fermentation broth. In the microbial synthesis of perillaldehyde fermentation broth, dodecane (dodecane:water = 1:9) is typically used for in-situ extraction to reduce the inhibitory effect of the product perillaldehyde on microorganisms. However, because the fermentation broth contains various amphoteric substances such as proteins and polysaccharides, forming an oil-in-water emulsion, the in-situ extraction effect is severely affected, cell activity decreases, and microbial fermentation is hindered. Therefore, timely oil-water separation of the emulsion is necessary. However, traditional demulsification techniques are time-consuming, costly, and cannot be well coupled with the extraction process. This invention, based on ceramic membrane oil-water separation, proposes the use of a coupled hydrophilic-oleophobic membrane and a hydrophobic-oleophobic membrane, assembled into a dual-membrane separator. This allows for the simultaneous permeation and separation of the oil and aqueous phases from the extracted emulsion, achieving continuous extraction and demulsification of the perillaldehyde fermentation broth, ensuring the continuous and efficient progress of biological fermentation.

The invention discloses a TPS / LPPS mutant for improving the yield of sclareol as well as an engineering bacterium and application of the TPS / LPPS mutant, and belongs to the technical field of synthetic biology and metabolic engineering. The invention provides a sclareol synthase (TPS) mutant and a danadiol diphosphate synthase (LPPS) mutant. The sclareol synthase (TPS) mutant and the danadiol diphosphate synthase (LPPS) mutant are obtained by carrying out random mutation on codon optimized TPS and LPPS genes through an error-prone PCR (Polymerase Chain Reaction) technology. The TPS mutant comprises one or more amino acid mutations in the 95th site, the 184th site, the 216th site, the 219th site, the 225th site, the 380th site, the 436th site and the 470th site; the LPPS mutant comprises one or more amino acid mutations in the 128th site, the 398th site, the 488th site and the 609th site. The invention also provides a recombinant engineering bacterium containing the mutant, preferably yarrowia lipolytica. The embodiment shows that the yield of sclareol is increased by 14-40% compared with that of a control group through the mutant obtained through screening. Technical support is provided for industrial production of sclareol, and the sclareol has a good application prospect.

The present invention discloses a quercetin-eucalyptus-oil composition for clearing and protecting the lungs, relating to the technical field of functional foods. The composition comprises active functional components and a three-phase layered targeted-delivery system. The active functional components comprise, in parts by weight, 5-20 parts quercetin, 1-8 parts eucalyptus oil, 2-10 parts bromelain, 10-30 parts dihydromyricetin, 1-6 parts perillaldehyde, 3-15 parts icariin, and 1-5 parts ultra-low-molecular-weight sodium hyaluronate. The three-phase layered targeted-delivery system comprises three independent encapsulation units, namely enteric-protective microspheres encapsulating bromelain, nano mixed micelles encapsulating quercetin, dihydromyricetin, and icariin, and sustained-release microcapsules encapsulating eucalyptus oil and perillaldehyde. By scientifically combining the seven active functional components with the three-phase layered targeted-delivery system and by improving the preparation process, the present invention solves the problems of component incompatibility and easy inactivation in conventional compositions, improves ingredient utilization, enables large-scale production, and enhances the storage stability of the composition.

The present invention discloses a method for producing perillaldehyde and / or perillyl lactone, comprising: contacting a first component with a second component to produce perillaldehyde and / or perillyl lactone; wherein the first component is selected from components containing lysine-2-diol, and the second component is selected from enzymes or microorganisms or their cell lysates or enzyme extracts capable of converting lysine-2-diol into perillaldehyde and / or perillyl lactone.