42 results about "Perillaldehyde" patented technology

The invention relates to the technical field of gel materials, and discloses whitening gel based on biological polysaccharide and a preparation method thereof, the whitening gel comprises the following raw materials: hyaluronic acid, deionized water and thunberg fritillary bulb polysaccharide microcapsules; wherein the bulbus fritillariae thunbergii polysaccharide microcapsule is prepared by taking konjac glucomannan, chitosan and perillaldehyde modified hyaluronic acid as a shell layer and embedding bulbus fritillariae thunbergii polysaccharide and glabridin as a core material; the perillaldehyde modified hyaluronic acid is prepared by reacting alkenyl hyaluronic acid with perillaldehyde under the action of an initiator; the alkenyl hyaluronic acid is prepared by reacting hyaluronic acid with glycidyl methacrylate under the action of a catalyst. The prepared whitening gel has excellent whitening, moisturizing, anti-oxidation and anti-aging effects, effective components are not prone to loss, and the whitening gel can be applied to the skin surface for a long time, plays a whitening role and delays the aging process of the skin.

This invention provides a genetically engineered bacterial strain, its preparation method, and its application in the synthesis of perillaldehyde. The genetically engineered bacterial strain includes the CcLPPS gene derived from *Cistus creticus*; the strain is selected from *Yarrowia lipolytica*. This invention constructs a stable, highly efficient de novo perillaldehyde synthesis strain. The entire fermentation process requires no addition of any organic reagents or intermediate substances, significantly reducing production costs and simplifying subsequent separation and purification steps. The engineered *Yarrowia lipolytica* strain provided by this invention exhibits high production intensity and a simple fermentation process, showing promising application prospects and laying the foundation for the microbial fermentation production of perillaldehyde.

The invention belongs to the technical field of biosynthesis, and particularly relates to a strain for producing sclareol as well as a construction method and application of the strain. In order to solve the problem of microbial synthesis of sclareol, phosphoketolase in an NOG pathway is mutated and optimized, phosphoketolase NaXpk from nanoarchaea archaeon is subjected to site-directed mutagenesis, a sclareol synthesis pathway is constructed in an escherichia coli host, the influence of NaXpk before and after mutation on the yield of sclareol is compared, and the yield of sclareol is improved. The S472F mutant and the S472Y mutant are determined, the S472F mutant and the S472Y mutant are applied to a sclareol production path, the sclareol yield of the finally obtained production strains can reach 623.3 mg / L and 655.2 mg / L, and the effect is remarkable.

The invention belongs to the technical field of medicine, and discloses a sea cucumber and ganoderma lucidum immune steady-state compound and a composite enzymatic hydrolysis targeted liposome co-delivery preparation process thereof.The sea cucumber and ganoderma lucidum immune steady-state compound is composed of sea cucumber, ganoderma lucidum, American ginseng, dried oyster meat, fructus lycii and perilla leaves; the ganoderma triterpenes release free polysaccharides and are combined with a liposome intestinal targeted delivery system, so that the absorption rate of the polysaccharides is greatly improved to 18-22% from 5% in the traditional process, the ganoderma triterpenes are emulsified and entrapped through a liposome double-layer membrane, and meanwhile, the permeation promotion effect of perillaldehyde in the perilla leaf volatile oil is supplemented, so that the transmembrane transport efficiency is remarkably enhanced, the absorption rate is improved to 12-15% from 2%, and the bioavailability is improved. The zinc element decomposes oyster zinc protein into free zinc ions through a composite enzymolysis technology, and the free zinc ions and vitamin C form a stable chelate, so that the antagonism of intestinal tracts is reduced, the zinc absorption rate is increased from 20% to 45-50%, and a solid foundation is laid for fully playing a compound immune regulation effect.

The invention discloses a sclareol synthase mutant, a recombinant genetic engineering strain and application of the sclareol synthase mutant, and belongs to the technical field of biology. The sclareol synthase is modified through semi-rational design, the obtained sclareol synthase mutant has good catalytic activity, and the yield of sclareol can be remarkably increased; furthermore, the method is simple. The method comprises the following steps: integrating an HMG1 gene for coding HMG-CoA reductase, an IDI1 gene for coding isopentenyl pyrophosphate isomerase, an SsGGPPS gene for coding geranyl-geranyl pyrophosphate synthase, an SsLPPS gene for coding lybaidan diglycol diphosphate synthase, and an SsGGPPS gene for coding lybaidan diglycol diphosphate synthase; a recombinant genetic engineering strain obtained by the nucleic acid molecule for coding the sclareol synthase mutant can produce sclareol at high yield by taking glucose as a substrate, and has a remarkable industrial application value.

The present invention discloses a method for producing perillaldehyde and / or perillyl lactone, comprising: contacting a first component with a second component to produce perillaldehyde and / or perillyl lactone; wherein the first component is selected from components containing lysine-2-diol, and the second component is selected from enzymes or microorganisms or their cell lysates or enzyme extracts capable of converting lysine-2-diol into perillaldehyde and / or perillyl lactone.

The invention relates to the technical field of traditional Chinese medicine detection, in particular to a method for determining the content of various components in blumea balsamifera oil. According to the method, a gas chromatography-mass spectrometry (GC-MS) technology is adopted, and chromatographic conditions are as follows: a chromatographic column is an SH-Stabilwax (30m * 0.32 mm, and the film thickness is 0.25 [mu] m) capillary column; the temperature of a sample inlet is 230 DEG C; the split ratio is 10: 1; the sample size is 1 microliter; the mass spectrum conditions are as follows: the ion source temperature is 200 DEG C; the interface temperature is 230 DEG C; the ionization mode is electron impact ion (EI); the detection voltage is 0.2 KV; the solvent is delayed for 2 minutes; the mass number scanning range is 30 to 400 amu; and SIM mode. According to the method disclosed by the invention, the contents of alpha-pinene, camphene, beta-pinene, limonene, p-cymene, camphor, alpha-coumarin, linalool, bornyl acetate, beta-caryophyllene, myrtenal, alpha-caryophyllene, L-borneol, perillaldehyde, caryophyllene oxide, guaiacol, beta-eudesmol and xanthoxylin in the blumea balsamifera oil can be simultaneously determined; the method provided by the invention has good precision, repeatability and stability, and the quality consistency of the blumea balsamifera oil can be quickly, accurately and effectively evaluated through detection of multiple components.

The invention discloses perillaldehyde liposome hydrogel as well as a preparation method and application thereof, and belongs to the technical field of medical biological materials. The preparation method comprises the following steps: firstly, preparing perillaldehyde liposome by taking lecithin, cholesterol and perillaldehyde as raw materials, and then assembling the perillaldehyde liposome in hydrogel to obtain the perillaldehyde liposome hydrogel. The prepared hydrogel has good stability and biocompatibility and can be applied to preparation of wound dressings, and perillaldehyde liposome hydrogel in the dressings can significantly accelerate wound healing by slowly releasing perillaldehyde, inhibiting inflammation and promoting collagen deposition.

The application discloses an antifungal effect of an antifungal emulsion and application of the antifungal emulsion in prevention and treatment of chestnut corruption, and belongs to the technical field of food preservation. The application is used for preventing and treating corruption caused by fungal infection during the collection and storage period of chestnuts. The antifungal emulsion comprises an antifungal component, an emulsifier and distilled water. The antifungal component comprises at least one of plant essential oil and active components of plant essential oil. The plant essential oil is at least one of thyme essential oil, clove essential oil and perilla essential oil. The active components of plant essential oil are at least one of thymol, eugenol and perillaldehyde. The application has good prevention and treatment effect on fungal infection of chestnuts during the collection and storage period, and has the advantages of being green, safe and efficient.

The invention discloses edible fresh-keeping gel beads for a vegetable protein beverage and a preparation method of the edible fresh-keeping gel beads, and belongs to the technical field of functional food and intelligent packaging. According to the edible fresh-keeping gel beads prepared by the preparation method disclosed by the invention, Schiff base formed by perillaldehyde-yeast protein is used as a liquid core, and Ca < 2 + > is used for pre-crosslinking; the wall material is a sodium alginate-rice protein composite network and can intelligently respond to imine bond protonation caused by pH drop in the beverage storage process, so that controllable release of perillaldehyde is realized, protein aggregation and microbial growth are inhibited, and a clean label and original sense are kept. According to the invention, triple advantages of edible, intelligent response and low cost are taken into consideration, and a plug-and-play fresh-keeping solution is provided for the plant-based protein beverage.

The invention provides a recombinant lipolytic yeast strain with high yield of sclareol as well as a preparation method and application of the recombinant lipolytic yeast strain. The recombinant yarrowia lipolytica strain can produce sclareol with high yield and efficiency, and effectively solves the problem of low yield of sclareol prepared by fermentation of existing recombinant yarrowia lipolytica. Meanwhile, when the recombinant lipolytic yeast strain is used for producing sclareol, the stability among batches is relatively good, and the requirements of large-scale industrial production on product stability can be met. Therefore, the recombinant lipolytic yeast strain disclosed by the invention has relatively high production benefits in the production of sclareol, so that the recombinant lipolytic yeast strain has a relatively good industrial application prospect.

This invention belongs to the field of biosynthesis technology, specifically relating to a strain for producing perillaldehyde, its construction method, and its application. Addressing the microbial synthesis problem of perillaldehyde, this invention mutates and optimizes the phosphotransketase in the NOG pathway, using a strain derived from nanoarchaemonas (…). Nanoarchaeota archaeon Site-directed mutagenesis was performed on the phosphoketolase NaXpk, and a perillaldehyde synthesis pathway was constructed in E. coli. The effects of NaXpk mutation on perillaldehyde yield were compared to identify the S472F and S472Y mutants. These mutants were then applied to the perillaldehyde production pathway, and the resulting production strains achieved perillaldehyde yields of 623.3 mg / L and 655.2 mg / L, respectively, demonstrating significant effectiveness.

The application discloses a kind of medicine food homologous sputum-removing and spleen-strengthening compositions and preparation method and application thereof, the composition includes: naringin 120-200 parts, hesperidin 80-100 parts, tuckahoe polysaccharide 150-250 parts, coix seed polysaccharide 80-120 parts, gingerol 10-20 parts, perillaldehyde 5-18 parts, glycyrrhizic acid 10-25 parts and glycyrrhizin flavone 8-24 parts by weight parts.The composition of the application can be prepared into medicine food homologous product, and it is not against disease, but against symptom, and has significant, stable and safe improvement effect on phlegm, throat discomfort, abdominal distension, thick and greasy tongue fur and other phlegm-related symptoms, without obvious toxic and side effects, and meets daily consumption requirements.

This invention relates to the field of agricultural product storage, and more particularly to a fumigation method for preventing soft rot in agricultural products. The method involves fumigation using a cellulose-based material as a carrier, perillaldehyde and / or salicylaldehyde as a base, and the addition of isobutyric acid and / or 2-methylbutyric acid. This invention effectively inhibits and kills *Rhizopus spp.*, the pathogen causing soft rot in agricultural products, maintaining the physiological quality and nutritional value of agricultural products during storage, and significantly extending the storage period. This invention is highly effective, simple to operate, environmentally friendly and safe, and easily scaled up and industrialized.

The invention belongs to the technical field of insect pest prevention and control, and particularly relates to application of a plant volatile matter to prevention and control of flower thrips, and the plant volatile matter comprises perillaldehyde and / or beta-pinene. Perillaldehyde and / or beta-pinene are / is separated from an aromatic plant perilla frutescens, has a remarkable repelling effect on the flower thrips, has a function of inducing natural enemies of the perilla frutescens, can effectively prevent and control the flower thrips, provides a scientific basis for a green prevention and control strategy of the flower thrips, development of plant-derived repellents and application of an ecological regulation and control technology, and has a wide application prospect. And the method has a relatively high application value.

This invention belongs to the field of biosynthesis, specifically relating to a variant of lysine pyrophosphate diol synthase and the constructed engineered yeast strain for perillaldehyde synthesis. To address the generally low yield of perillaldehyde biosynthesis in existing technologies, this invention mutates and optimizes the lysine pyrophosphate diol synthase to obtain an SsLPPs mutant containing the G379M mutation, which is then used for perillaldehyde synthesis. Simultaneously, to address the genetic instability issues associated with free plasmid expression in some Saccharomyces cerevisiae strains, this invention constructs a perillaldehyde synthesis pathway within the yeast genome. Furthermore, by enhancing the acetyl-CoA synthesis pathway, the MVA pathway, and the perillaldehyde synthesis pathway, and by knocking out some transcriptional repressors, the substrate strain synthesizing perillaldehyde is modified, resulting in a significantly increased yield compared to existing technologies.

The application provides a use of perillaldehyde in preparation of a medicine for treating postpartum lack of lactation, and animal experiments show that perillaldehyde can effectively increase the lactation of postpartum lack of lactation female mice, significantly increase the litter weight growth rate of postpartum lack of lactation female mice, and improve the pathological state of mammary gland tissue of postpartum lack of lactation female mice. In addition, perillaldehyde has good safety and almost no side effects on the body. Therefore, the perillaldehyde described in the application can be used as an active ingredient and applied to preparation of a medicine for treating postpartum lack of lactation, thereby developing a new use of perillaldehyde and providing a new selection for preparation of a medicine for promoting lactation secretion and treating postpartum lack of lactation.

The invention belongs to the field of biosynthesis, and in particular relates to an improved variant of libdanenediol pyrophosphate synthase and engineered yeast for producing sclareol. In order to solve the problem that in the prior art, the sclareol biosynthesis yield is generally low, LsLPPs mutant containing N674L mutation is obtained by performing mutation and optimization on LsLPPs pyrophosphate synthase, and the mutant is used for sclareol synthesis. Meanwhile, in order to solve the problem of genetic instability caused by free plasmid expression of part of saccharomyces cerevisiae strains, a sclareol synthesis pathway is constructed in a yeast genome, and meanwhile, an acetyl coenzyme A synthesis pathway, an MVA pathway and a sclareol synthesis pathway are enhanced; and the chassis bacteria for synthesizing sclareol are modified in a manner of knocking out part of transcription inhibition factors, so that the yield is greatly improved compared with the prior art.

The invention discloses a perillaldehyde formylhydrazine compound and a preparation method and application thereof.The preparation method comprises the steps that perillaldehyde is subjected to an oxidation reaction to synthesize perillaldehyde, the perillaldehyde reacts with phenylhydrazine hydrochloride with different substituent groups to synthesize the perillaldehyde formylhydrazine compound, the compound has a general formula I, and R is H, 4-F, 3-F, 2-F, 4-Cl, 3-Cl, 2-Cl, 4-Br, 3-Br, 2-Br, 4-I, 4-CH3, 4-OCH3 and 2-Cl-4-F; the compound has a good prevention effect on pythium aphanidermatum and botrytis cinerea under an in-vitro condition, and can be used for preventing and treating fungal diseases of agricultural or forestry plants. The preparation method of the compound is simple and convenient, the yield is high, and the product property is stable.

The invention belongs to the field of food additives, and particularly discloses a blended houttuynia cordata flavor essence and a preparation method thereof.The blended houttuynia cordata flavor essence comprises, by mass, 3%-6% of 2-decenal, 2%-5% of 2-nonenal, 2%-6% of 2, 4-dihydroxy-2-methyl-1, 3-pentanedione, 1%-3% of 2, 4-dihydroxy-2, 4-pentanedione, 1%-3% of The formula comprises the following components in percentage by weight: 1%-3% of 1, 4-decadienal, 3%-5% of 2-decanone, 1%-3% of lauric aldehyde, 1%-2% of myrcene, 4%-7% of beta-caryophyllene, 0.5%-1.5% of cis-3-hexene-1-ol, 2%-3% of 2-hexenal, 2%-5% of linalool, 0.5%-1.5% of 1-octylene-3-ol, 0.5%-1.5% of perillaldehyde, 2%-3% of 2-amyl furan, 1%-3% of eucalyptus oil, 1%-2% of eugenol and the balance of water. The composition is prepared from 0.5%-1.5% of methyl dihydrojasmonate, 3%-5% of cis-6-nonene-1-ol, 1%-3% of 2-acetylpyridine, 1%-3% of cis-6-nonene-1-ol, 1%-3% of 2-acetylpyridine, 1%-3% of cis-6-nonene-1-ol, 1%-3% of vetiver oil and the balance of caprylic capric triglyceride. The blended houttuynia cordata flavor essence is prepared from the monomer spice for food, and has the characteristics of obvious characteristic fragrance, strong natural feeling, long fragrance retention time and high temperature resistance.

The application discloses a method for recovering perillyl alcohol crude product from perillyl alcohol mother liquor, comprising the following steps: (1) perillyl alcohol in the perillyl alcohol mother liquor is deoximated under the catalysis of acid to generate perillyl aldehyde crude product; (2) perillyl aldehyde is subjected to distillation and impurity removal to obtain perillyl aldehyde with high content; (3) the purified perillyl aldehyde is added into a hydroxylamine solution to perform oximation reaction, and perillyl alcohol crude product can be obtained after the reaction. The method can remove impurities through the distillation method after the perillyl alcohol mother liquor is converted into perillyl aldehyde crude product, and then the oximation is performed again to obtain perillyl alcohol crude product. About 50% of perillyl alcohol in the perillyl alcohol mother liquor can be recovered, and the total yield of perillyl alcohol industrial production is increased.

The invention relates to the technical field of biological medicine, in particular to combined application of perillaldehyde and givelastat in preparation of a medicine for treating gastric cancer. The invention provides combined application of perillaldehyde and givelastat in preparation of a medicine for treating gastric cancer. Experiments prove that the perillaldehyde and the givelastat are combined to remarkably inhibit growth of gastric cancer cells and promote apoptosis, so that the perillaldehyde and the givelastat have a wide prospect in development of gastric cancer treatment medicines.

The application discloses a quercetin-eucalyptus oil composition for clearing and protecting lungs, and relates to the technical field of functional food. The composition comprises an active functional component and a three-phase layered targeting delivery system. The active functional component is composed of quercetin 5-20 parts, eucalyptus oil 1-8 parts, bromelain 2-10 parts, dihydromyricetin 10-30 parts, perillaldehyde 1-6 parts, icariin 3-15 parts and ultralow-molecular-weight sodium hyaluronate 1-5 parts in terms of weight fraction. The three-phase layered targeting delivery system comprises three independent embedding units, namely enteric-soluble protective microspheres for wrapping bromelain, nano-mixed micelles for wrapping quercetin, dihydromyricetin and icariin, and slow-release microcapsules for wrapping eucalyptus oil and perillaldehyde. The application solves the problems of component compatibility conflict and component inactivation in traditional compositions by scientifically matching seven active functional components and combining the three-phase layered targeting delivery system, improves the utilization rate of components, can be produced on a large scale, and improves the storage stability of the composition.

The invention discloses a preparation method and application of perillaldehyde, and belongs to the technical field of organic compound synthesis, limonene is used as a substrate, potassium hydrogen persulfate composite salt is used as an oxidizing agent, limonene 1, 2-epoxide is prepared through an oxidation reaction in the presence of a surfactant, then isomerization reaction is performed to prepare iso-carveol, and the iso-carveol is obtained. Isomerization reaction is carried out on the iso-carveol again to prepare perillyl alcohol, and oxidation reaction is carried out again to prepare perillaldehyde. According to the invention, through the cascade amplification effect, the yield can be increased stage by stage in four-stage reaction, finally, the yield is obviously increased, the yield of single-step reaction can be increased by 50%, and the production cost is reduced. The method is suitable for preparing perillaldehyde and analogues thereof, and is used for preparing food additives such as perillaldehyde perfume, drug intermediates or preservatives.

This invention provides a recombinant lipolytic yeast strain with high perillaldehyde production, its preparation method, and its applications. This recombinant lipolytic yeast strain can produce perillaldehyde with high yield and efficiency, effectively solving the problem of low yield in existing recombinant Yersinia lipolytic yeast fermentation for perillaldehyde production. Furthermore, the batch-to-batch stability of perillaldehyde production using this recombinant lipolytic yeast strain is good, meeting the requirements for product stability in large-scale industrial production. Therefore, the recombinant lipolytic yeast strain of this application has high production efficiency in perillaldehyde production, thus showing good prospects for industrial application.

The invention discloses a green compound bactericide for preventing and treating apple moldy core and a preparation method thereof, and relates to the technical field of apple disease prevention and control, and the preparation method specifically comprises the following steps: S1, preparing epsilon-polylysine microspheres; s2, preparing a perillaldehyde nano-liposome; s3, preparing a rhodovulum sulfidophilum fermentation solution; s4, compounding a finished product; and S5, enzyme sensitive coating. According to the compound bactericide disclosed by the invention, 1 + 1gt can be realized through a multi-mechanism synergistic effect (targeted delivery, direct bacteriostasis, induced resistance and ecological occupation); and 2, the composition has the characteristics of green, safety, high efficiency and stability.

This invention provides an engineered microorganism and a method for synthesizing perillaldehyde. The engineered microorganism comprises a gene for perillaldehyde synthase and a gene for Bayer-Villiger monooxygenase. The perillaldehyde synthase comprises: (a1) a protein having any sequence in SEQ ID NOs: 1-25, or; (a2) a protein having more than 70% homology with the amino acid sequence defined in (a1) and possessing perillaldehyde synthase activity. The Bayer-Villiger monooxygenase comprises: (b1) a protein having any sequence in SEQ ID NOs: 26-50, or; (b2) a protein having more than 70% homology with the amino acid sequence defined in (b1) and possessing Bayer-Villiger monooxygenase activity. This invention solves the problem of inefficient biosynthesis of perillaldehyde in existing technologies and is applicable to the field of synthetic biology.

The invention relates to the technical field of microorganisms, in particular to a method for improving quality and insect resistance of purple perilla through inoculation of arbuscular mycorrhizal fungi, which is characterized in that the arbuscular mycorrhizal fungi are rhizosporium endophyllum, and insects are beet armyworms. The arbuscular mycorrhizal fungi are inoculated, so that the growth of the aboveground part and the underground part of the purple perilla is remarkably promoted, and the biomass of the purple perilla is improved; accumulation of linalool, perillyl alcohol, perillaldehyde, elemene, ocimene and limonene in the perilla leaves can be promoted, and the quality of the perilla is improved; the content of defense secondary metabolites such as flavone and tannin of the perilla leaf and the activity of defense enzymes such as polyphenol oxidase and phenylalanine ammonialyase can be improved, and the resistance of the perilla leaf to beet armyworms is improved. After the arbuscular mycorrhizal fungi inoculated perilla leaves are eaten, the growth and development of the beet armyworms are inhibited, and the beet armyworms show that the weight and the body length are reduced, and the developmental duration is prolonged.

This invention relates to the field of tobacco technology, specifically to a flavored tobacco extract, its preparation method, and its application. The flavored tobacco extract provided by this invention comprises: ceftazidimetrienol, megastigmatrienone, nicotine, β-ionone, 4-hydroxy-β-dihydrodamascone, isovaleric acid, 2-methylbutyric acid, and perillaldehyde; the mass ratio of ceftazidimetrienol, megastigmatrienone, nicotine, β-ionone, 4-hydroxy-β-dihydrodamascone, isovaleric acid, 2-methylbutyric acid, and perillaldehyde in the flavored tobacco extract is (1.5-3.3):(0.1-0.25):(3.5-15):(0.0015-0.01):(0.01-0.1):(0.05-0.5):(0.005-0.02):(0.05-0.4). The aromatic tobacco extract provided by this invention has high safety, while the extract has a distinct aroma, good overall harmony, and a good overall sensory experience.