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Application of perillaldehyde in preparation of medicine for preventing and treating vaginitis

A technology of perillaldehyde and vaginitis, applied in the new application field of perillaldehyde

Inactive Publication Date: 2017-10-10
XUZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there are no reports on the prevention and treatment of Candida albicans vaginitis by perillaldehyde at home and abroad

Method used

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  • Application of perillaldehyde in preparation of medicine for preventing and treating vaginitis
  • Application of perillaldehyde in preparation of medicine for preventing and treating vaginitis
  • Application of perillaldehyde in preparation of medicine for preventing and treating vaginitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: Perillaldehyde is to the mensuration of Candida albicans minimum inhibitory concentration (MIC)

[0022] The minimum inhibitory concentration (MIC) of perillaldehyde against clinically isolated Candida albicans was determined according to the M27-A3 microbroth dilution method formulated by the National Committee for Clinical Laboratory Standards (NCCLS).

[0023] The test medium is RPMI1640.

[0024] The pathogenic fungus tested was Candida albicans (n=6).

[0025] Using the double dilution method, put 100 μL RPMI1640 medium into each well of the 96-well plate, then add 100 μL perillaldehyde for doubling dilution, and obtain the concentration range of perillaldehyde from the 1st to the 10th well of the 96-well cell culture plate From 0.00313 to 3.2 μL / mL. Re-inoculate 100 μL 1×10 3 cfu / mL bacterial suspension, so that the final volume of each well is 200 μL, add only 200 μL of RPM11640 medium to the 11th well as the medium control well, add 200 μL of Ca...

Embodiment 2

[0026] Example 2: The preventive effect of perillaldehyde on Candida albicans vaginitis

[0027] Perillaldehyde has a preventive effect on Candida albicans vaginitis caused by Candida albicans.

[0028] 1. Experimental materials

[0029] The test medium is RPMI1640 medium.

[0030] The pathogenic fungus tested was Candida albicans.

[0031] The test animals were female BALB / c (22±2g) mice.

[0032] 2. Experimental Preparation

[0033] Preparation of Candida albicans bacterial suspension: Candida albicans was inoculated in Sandcastle Broth (SDB), cultured at 37°C for 12 hours, centrifuged at 3200g for 5 minutes, collected the bacteria, diluted with RPMI1640 medium, passed through hemocytometer The counting plate adjusted the concentration of the bacterial suspension to 6×10 6 cfu / mL.

[0034] Preparation of perillaldehyde: Use 0.01% sterile Tween-20 containing 1% carboxymethylcellulose sodium (CMC-Na) to prepare different concentrations of perillaldehyde (0.4 μL / mL, 0.8 μ...

Embodiment 3

[0051] Embodiment 3: the therapeutic effect of perillaldehyde on Candida albicans vaginitis

[0052] Perillaldehyde has a therapeutic effect on Candida albicans vaginitis caused by Candida albicans.

[0053] 1. Experimental materials

[0054] The test medium is RPMI1640 medium.

[0055] The pathogenic fungus tested was Candida albicans.

[0056] The test animals were female BALB / c (22±2g) mice.

[0057] 2. Experimental Preparation

[0058] Preparation of Candida albicans bacterial suspension: Candida albicans was inoculated in Sandcastle Broth (SDB), cultured at 37°C for 12 hours, centrifuged at 3200g for 5 minutes, collected the bacteria, diluted with RPMI1640 medium, passed through hemocytometer The counting plate adjusted the concentration of the bacterial suspension to 6×10 6 cfu / mL.

[0059] Preparation of perillaldehyde: Use 0.01% sterile Tween-20 containing 1% carboxymethylcellulose sodium (CMC-Na) to prepare different concentrations of perillaldehyde (0.4 μL / mL, ...

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Abstract

The invention discloses an application of perillaldehyde in preparation of a medicine for preventing and treating vaginitis, and belongs to the technical field of medicines. The invention further discloses a medicine for preventing and treating candida albicans vaginitis, which is a preparation prepared by using perillaldehyde as an active ingredient with pharmaceutically acceptable auxiliaries. The perillaldehyde has a relatively strong effect for inhibiting candida albicans. An in-vivo experiment is carried out by adopting a mouse immunosuppression model to evaluate the effect of perillaldehyde for preventing and treating candida albicans vaginitis; and fungus inspection and tissue histopathologic examination prove that perillaldehyde has relatively strong effects for preventing and treating candida albicans vaginitis, and can be used for preparing the medicine for preventing and treating candida albicans vaginitis.

Description

technical field [0001] The invention relates to a new application of perillaldehyde, which belongs to the technical field of medicine. Background technique [0002] Candida albicans is an important opportunistic pathogen that mainly exists in the oral cavity, upper respiratory tract, intestinal tract, and vagina of the human body. It can not only cause skin or mucous membrane lesions, but also cause systemic infections in the human body. Among them, Candida albicans vaginitis caused by Candida albicans is a relatively common gynecological disease that occurs among women in today's society. The typical symptoms of this disease are phlegm itching and increased vaginal discharge, usually accompanied by vaginal Symptoms such as pain, vulvar burning, dyspareunia, and painful urination. This disease can be transmitted by sexual intercourse. About 70% of women of childbearing age have suffered from at least one or more cases of Candida albicans vaginitis, and there will be some Pa...

Claims

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Application Information

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IPC IPC(8): A61K31/11A61P15/02
CPCA61K31/11
Inventor 田慧瞿素李瑾婷汪丽琴季惠席刘荣田俊
Owner XUZHOU NORMAL UNIVERSITY
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