20 results about "Opportunistic pathogen" patented technology

This invention discloses a probiotic liquid core ball cultured under polysaccharide confinement, its preparation method, and its application in improving children's intestinal flora. Belonging to the field of probiotic preparation technology, it employs an extrusion method combined with calcium alginate cross-linking technology, using xanthan gum and other thickening polysaccharides to optimize the types and concentrations of polysaccharides to construct the confinement space. The effects of different oxygen conditions on the encapsulation of different proportions of *Lactobacillus rhamnosus* LRa05 and *Bifidobacterium animalis* BLa80 were investigated to obtain the optimal probiotic liquid core ball cultured under polysaccharide confinement. This process is simple, uses safe raw materials, and after confinement culture, the viable bacteria count is ≥10⁹ CFU / g. It can directionally increase the abundance of beneficial bacteria in children's intestines, inhibit opportunistic pathogens, enhance flora diversity and stability, and is suitable for children's dietary scenarios, providing a safe and efficient intestinal flora regulation solution.

The application provides a device for synergistically controlling disinfection by-products and conditional pathogenic bacteria of surface water by a low-temperature-resistant bank filtration-nanofiltration combined process and a working method thereof, and belongs to the technical field of water treatment and efficient water resource utilization. An aeration disc is arranged at the inner bottom of a raw water tank in the device, a bank filtration simulation column is filled with multiple layers of filter materials, the outlet end of the raw water tank is communicated with the water inlet of the bank filtration simulation column through a peristaltic pump, the water inlet of the bank filtration simulation column is located above the bank filtration simulation column, the water outlet at the lowermost part of the bank filtration simulation column is communicated with the water inlet of a bank filtration water collecting tank, the water outlet of the bank filtration water collecting tank is communicated with the water inlet of a nanofiltration membrane assembly, the clean water outlet of the nanofiltration membrane assembly is communicated with a nanofiltration water collecting tank, and the concentrated water outlet of the nanofiltration membrane assembly is communicated with a nanofiltration concentrated water tank. The device can solve the problem of unstable water quality of bank filtration caused by environmental factors, and can also alleviate the membrane pollution problem of nanofiltration technology through bank filtration pretreatment.

This invention relates to the fields of microbial genetic engineering and brewing technology, and provides a PDC1 gene mutant strain resistant to 2-phenylethanol and its application in the brewing of wheat-aroma baijiu. The mutant strain is *Saccharomyces cerevisiae* PDC1_473 (HM 03), with the preservation number CCTCC M20252996. The PDC1 gene of this strain has a base mutation from T to C at position 473. When this mutant strain is applied to the solid-state fermentation of wheat-aroma baijiu, it becomes the absolutely dominant fungal community, significantly altering the microbial community structure of the fermentation system, inhibiting the growth of various conditionally pathogenic and spoilage bacteria, strengthening the fermentation environment centered on *Lactobacillus*, and significantly enhancing the rose, citrus, grassy, ​​fatty, and caramel aromas of the baijiu. It imparts a pleasant, rich ester aroma and complex aroma to the baijiu, increasing its fullness, and has broad application potential in the brewing of wheat-aroma baijiu.

This invention provides a kit and detection method for detecting the Siglec fusion mutation gene and the GBS pathogenic gene. It is used for rapid combined detection of the Siglec5 / 14 fusion mutation gene, a preterm birth susceptibility gene, and the GBS pathogenic gene. It can effectively assess the risk of gynecological reproductive tract infections, preterm birth in pregnant women, and infection in newborns. It solves the problem that existing kits only detect the conditionally pathogenic bacteria and have poor specificity for disease association. The detection using this kit is faster and more sensitive. The detection process is closed-loop, avoiding the additional operation of electrophoresis differentiation. It is low-cost and easy to promote.

The invention relates to the technical field of microorganisms and biological medicines, in particular to triple probiotics as well as a preparation method and application thereof, and the triple probiotics comprise at least one probiotic of bacillus, lactobacillus acidophilus and bifidobacterium longum. The triple probiotics provided by the invention form an'oxygen consumption-enzyme production-acid production 'synergistic chain, and through synergistic degradation of uric acid, inhibition of excessive growth of conditioned pathogens and enhancement of intestinal barrier functions, the blood uric acid level of patients with asymptomatic hyperuricemia is reduced, the alpha-diversity of intestinal tracts is improved, the abundance of butyric acid producing bacteria is improved, and the content of the butyric acid producing bacteria is improved. The ratio of escherichia coli to shigella is reduced; sIBO is effectively relieved or reversed, and digestive tract symptoms such as abdominal distension and exhaust are reduced. In addition, the triple probiotics avoid liver and kidney toxicity caused by chemical drugs, and are suitable for long-term administration.

The invention discloses a next-generation sequencing kit for detecting respiratory tract pathogenic bacteria based on multiple primer amplification, and belongs to the technical field of microbiological detection. The nucleotide sequence of the primer group is as shown in SEQ ID NO.1-2670. The primer group is used for detecting classification of various pathogens, drug-resistant gene loci and host infection reaction up-regulation genes by adopting a targeted high-throughput sequencing (tNGS) technology. Compared with metagenome high-throughput sequencing (mNGS), the method has the advantages that the detection sensitivity is greatly improved, DNA and RNA processes can be considered at the same time, the detection time is greatly shortened, and the method has the characteristics of accuracy, practicability, uniqueness, leading edge, time saving, money saving and the like; especially, detection on host infection reaction up-regulation genes can be used for distinguishing pathogenic bacteria or conditioned pathogenic bacteria in samples, distinguishing whether acute infection causes belong to infectivity or non-infectivity, is unique in similar metagene products, and is compared with a metagene mNGS method for clinical specimen comparison verification. And the kit is accurately applied to etiological diagnosis of respiratory system infection.

The application discloses a fermentation feed feeding method for promoting growth of Ictalurus punctatus and improving intestinal flora. When cultivated, the Ictalurus punctatus is fed with puffed compound feed and fermentation feed; every other week, 20% of the puffed compound feed is replaced by the fermentation feed, and the mixed feed is fed for one week, and the feed is fed twice a day in the morning and in the evening. When cultivated by the method, the weight of the Ictalurus punctatus in the experimental group with the added fermentation feed is higher than that in the control group, and the weight of the Ictalurus punctatus in the interval feeding group is the highest and is significantly higher than that in the control group. Because the fermentation feed is continuously added, the diversity and richness of the intestinal flora of the Ictalurus punctatus decrease, especially the abundance of the actinobacteria and cyanobacteria, and the relative abundance of the opportunistic pathogen proteobacteria is increased, thereby affecting the growth of the Ictalurus punctatus.

The invention relates to an opportunistic pathogenic bacterium infection diagnosis biomarker group and application thereof, a diagnosis product and a diagnosis method, and relates to the fields of opportunistic pathogenic bacterium infection diagnosis technology and precision medical treatment. The biomarker group comprises an mRNA (messenger ribonucleic acid) of an ANKRD52 protein, an mRNA of a ZC3HAV1L protein, an mRNA of an SERPINE3 protein, an mRNA of a CDPF1 protein, an mRNA of a ZNF720 protein, an mRNA of a TAGLN3 protein and an mRNA of an LRRC15 protein. The diagnostic product comprises a reagent for detecting the mRNA level of each marker in the biomarker group. The diagnosis method comprises the following steps: acquiring the RPM value of microorganisms in a sample to be detected and the mRNA expression level (TPM value) of the seven proteins as input characteristics, and judging the diagnosis result of the sample to be detected according to the output result of the opportunistic pathogenic bacterium infection random forest algorithm diagnosis model.

The present invention relates to a bacterial preparation for the treatment of hatchery environment, which contains as an active ingredient a mixture of bacteria Chryseobacterium indologenes and Sphingobacterium multivorum. The invention further relates to the use of said preparation for the preventive improvement of the physiological condition of poultry, in particular for the prevention of infections of poultry by opportunistic pathogens selected from the group comprising E. coli, Clostridium sensu stricto. Enterococcus, Klebsiella, Enterobacter and Pseudomonas.

The invention discloses an application of a bacterial strain in production of an external product for improving the micro-ecology of the vagina of a human body, and the bacterial strain is Lactobacillus crispatus SPR003 and has 16S rDNA (ribosomal deoxyribonucleic acid) as shown in SEQ ID NO.1. The invention further discloses a preparation method of the bacterial strain for improving the micro-ecology of the vagina of the human body. The preservation number is GDMCC NO: 66664; the strain produces H2O2 and only produces D-lactic acid, and no drug-resistant gene is found; the external product is suitable for improving changes, caused by vaginal pathogenic bacteria, of micro-ecology of the vagina of the human body. Compared with other lactobacillus crispatus of the same type, the strain has the advantages that the H2O2 production capacity is high, the H2O2 production speed is high, the total amount of H2O2 produced within 12 hours is large, and the concentration of hydrogen peroxide can reach about 220 mu M / L after the strain is cultured for 12 hours in the conventional inoculation amount of 1%. H2O2 is a strong oxidant, and can directly kill or inhibit a plurality of pathogenic bacteria and conditioned pathogenic bacteria such as Gardner bacteria, campylobacter actinomycetes, prevotella and other BV-related anaerobic bacteria.

Use of an N-acetylneuraminic acid substance in the preparation of a food product for promoting the regulation of gut microbiota of a mother and offspring thereof via ingestion by the mother. The N-acetylneuraminic acid substance is an N-acetylneuraminic acid anhydrate, an N-acetylneuraminic acid hydrate or an N-acetylneuraminic acid salt. The mother is in a gestational period and / or lactation period, and the offspring is in a fetal stage, infant stage and / or toddler stage. The mother starting to ingest the N-acetylneuraminic acid substance in the gestational period can promote the increase of beneficial bacteria in the gut in the gestational and lactation periods, and can also increase the content of probiotics in the offspring gut, inhibit the proliferation of opportunistic pathogens in the offspring gut and reduce the relative abundance thereof, thereby further contributing to maintaining the health of the nervous system by means of the action of the gut-brain axis.

The invention relates to an application of bifidobacterium animalis subsp. Lactis BLa80 in preparation of a preparation for relieving obesity. The bifidobacterium animalis subsp. Lactis BLa80 is a strain of the bifidobacterium animalis subsp. Lactis BLa80 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.22547, and the strain of the bifidobacterium animalis subsp. Lactis BLa80 is a strain of the bifidobacterium animalis subsp. Lactis BLa80 with the preservation number of CGMCC No.22547. The brand new application of the BLa80 strain of the animal bifidobacterium subsp. Lactis is developed, that is, the BLa80 strain is used for improving obesity, specifically, the BLa80 strain can effectively control the weight gain of obese mice and reduce the obesity index; fat accumulation, liver injury and in-vivo inflammation caused by obesity are improved; the intestinal flora structure of an obese organism can be improved, the abundance of beneficial flora in the intestinal tract is increased, and the abundance of conditioned pathogenic bacteria is reduced.

Described herein are synthetic glycopolypeptides that contain sugars identified as active in mucin. These compounds reduce the virulence of high-priority opportunistic pathogens, including, but not limited to, Salmonella enterica subsp. enterica serovar Typhimurium, Candida albicans, and Gardnerella vaginalis, without reducing the viability of beneficial Lactobacillus crispatus. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.

The invention discloses a traditional Chinese medicine composition for treating rheumatoid arthritis, and relates to the technical field of traditional Chinese medicines, and the traditional Chinese medicine composition is prepared from the following raw material medicines in parts by weight: 5-15 parts of coptis chinensis, 3-10 parts of rheum officinale, 10-20 parts of radix paeoniae alba, 10-25 parts of caulis sinomenii, 5-15 parts of dark plum, 5-10 parts of liquorice, 15-30 parts of rhizoma drynariae and 10-20 parts of herba epimedii. An intestinal tract immunity source regulating function is constructed in a traditional Chinese medicine compound, berberine in coptis chinensis and anthraquinone components in rheum officinale serve as potential prebiotics, proliferation of conditioned pathogenic bacteria such as gram-negative bacteria is selectively inhibited in intestinal tracts, colonization of beneficial bacteria such as lactobacillus and bifidobacterium is promoted, and the intestinal tract immunity regulating function is achieved. The process directly increases the yield of short-chain fatty acids in the intestinal tract, and metabolites such as butyrate and the like can enter the circulatory system through the intestinal wall, so that differentiation and functions of regulatory T cells in the body are promoted, excessive activation of helper Th17 cells is inhibited, and intervention is performed from the source of immunity.

The invention relates to a compound probiotic formula for vegetarian birds, which consists of the following components in parts by weight: 300 parts of clostridium butyricum; 95 parts by weight of bacillus subtilis; 100 parts by weight of bacillus licheniformis; 25 parts by weight of amylase; 50 parts by weight of papain and 50 parts by weight of phytase; 25 parts by weight of xylanase; 250 parts by weight of beta glucanase; 100 parts by weight of mannase; 70 parts by weight of cellulase; and 70 parts by weight of glucose. The feed has the advantages that the compound probiotics in the formula are supplemented to young birds, so that gastrointestinal motility can be promoted, and weaning is assisted; birds are often treated by broad-spectrum antibiotics after bacterial infection, original intestinal flora is destroyed to cause secondary infection, and after the antibiotics are used, the probiotics in the formula are taken, so that the probiotics can be colonized in intestinal tracts, antibacterial active substances are generated, new intestinal flora is established, and conditioned pathogenic bacteria are prevented from excessively growing.

A nutritional composition comprising digestible carbohydrates, protein and lipid, wherein the lipid is in the form of lipid globules which are at least partly coated on the surface with phospholipids, for improving the gut microbiota and reducing opportunistic pathogens in the gut of human infants.

This invention proposes a microscopic imaging wind tunnel for environmental precipitation and its application in health risk assessment. The wind tunnel simulates precipitation through a precipitation generation module connected to the air. Through meteorological data acquisition, regional gridding, and output of simulated precipitation generation data, the air quality response to precipitation is observed. Following the steps of precipitation simulation, mixing and diffusion, and microscopic observation, the three-dimensional migration behavior of bioaerosols is observed to conduct health risk assessment, solving the challenges of in-situ observation and tracing microscopic imaging of the enrichment of diffused aerosols in ambient air and the apoptosis and proliferation of live cells. The wind tunnel application described in this invention for health risk assessment is simple, easy to implement, and widely applicable. It facilitates the dynamic monitoring of biological pollutants moving with the atmosphere under conditions such as rainfall, snowfall, hail, and typhoons. It is suitable for providing scientific data on the spread of conditionally pathogenic bacteria in residential areas, landfills, and other areas, and has strong relevance and practicality.

The invention discloses application of inorganic nitrate gel in prevention and treatment of microgravity gastrointestinal injury, and relates to the field of medicine and pharmaceutics. The nitrate gel comprises 2-7wt% of nitrate microcapsules which are entrapped and dispersed in a hydroxypropyl methyl cellulose gel matrix in the form of hydrolyzed whey protein, pectin and gelatin composite microcapsules, so that the abundance of microgravity-related beneficial bacteria Dysosmobacter and Adelleria can be remarkably improved, the microgravity-related microgravity-related beneficial bacteria can be remarkably improved, the microgravity-related microgravity-related microgravity-related microgravity-related microgravity-related microgravity-related microgravity-related microgravity-related microgravity-related microgravity-related microgravity-related microgravity-related microgravity-related microgravity-related meanwhile, the growth of conditioned pathogens Escherichia and enterococcus in a microgravity environment can be remarkably inhibited, necrosis or apoptosis of gastrointestinal mucosa cells caused by microgravity can be reduced, the protection effect on gastrointestinal tracts in the microgravity environment is achieved, and then microgravity gastrointestinal injury is prevented or treated. Long-term toxicity tests prove that the traditional Chinese medicine composition has no adverse effects on important organs such as heart, liver, kidney and the like.

A nutritional composition comprising a digestible carbohydrate, a protein and a lipid wherein the lipid is in the form of lipid globules which are at least partially coated on the surface with phospholipids for use in improving the gut microbiota and reducing opportunistic pathogens in the gut of a human infant.

This invention discloses a dietary fiber from kiwifruit peel residue with different levels of polyphenol release, its preparation method, and its applications. Using kiwifruit peel residue as raw material, the starch and protein are removed by enzymatic hydrolysis to obtain dietary fiber. Then, alkaline hydrolysis and combined alkaline hydrolysis with ultrasonic treatment are used to prepare kiwifruit peel residue dietary fiber samples with different levels of polyphenol release. The release amount of polyphenols, changes in antioxidant activity, intestinal flora regulation, and short-chain fatty acid production capacity of each sample were evaluated. The A8MUS sample significantly increased the relative abundance of beneficial bacteria such as Bacteroides, decreased the relative abundance of conditionally pathogenic bacteria such as Escherichia coli and Providencia, and promoted the production of short-chain fatty acids such as acetic acid and isobutyric acid. This invention realizes the high-value utilization of kiwifruit peel residue by-products, and the obtained dietary fiber can be used as a functional food ingredient or an intestinal microecological regulator.