53results about How to "Improve antagonistic ability" patented technology

The invention provides a sugarcane sustained-release granular medicinal fertilizer comprising the components of, by mass: 0.01-3% of a pesticide, 20-50% of fertilizer effective components, 0.01-2% of trace element fertilizer effective components, 0.001-0.1% of beneficial element fertilizer effective components, 0.1-5% of a fertilizer sustained-release agent, and balance of a filling agent. The fertilizer effective components comprise N, P2O5, and K2O. The trace element fertilizer effective component comprises boron, zinc, manganese, molybdenum, copper and iron. The beneficial element fertilizer effective components comprise silicon and selenium. The sugarcane special-purposed sustained-release granular medicinal fertilizer provided by the invention can provide nutrients for sugarcane, and can control pests effectively. With the medicinal fertilizer, application effects of pesticide and fertilizer are improved, labor intensity is saved, and plantation cost is reduced.

The invention discloses a compound microbial fertilizer for antagonism of soil-borne fungal diseases, and a preparation method and an application thereof; the fertilizer takes humic acid as a compound carrier, includes bacillus amyloliquefaciens HFJ-7 with the bacteria content of 1.8*10<8>-2.1*10<8> CFU/g, and also includes bacillus megatherium B3. The bacillus amyloliquefaciens HFJ-7 in the compound microbial fertilizer has the preservation number of CGMCC No.10011. The bacillus amyloliquefaciens HFJ-7 strain can be antagonistic to rhizoctonia solani, fusarium oxysporum, root rot pathogen, cucumber fusarium wilt pathogen, phytophthora capsici leon pathogen and other soil-borne disease pathogenic fungi. After the bacillus amyloliquefaciens HFJ-7 is compounded with the bacillus megatherium B3, the resistance to rhizoctonia solani and pepper root rot pathogen can be significantly improved, a disease state index of pepper seedling root rot disease can be significantly reduced, and the occurrence and spreading of pepper root rot can be effectively prevented and controlled.

The invention relates to a Trichoderma strain for antagonizing soil-borne disease, belonging to the field of biological control. The Trichoderma strain is Trichoderma asperellum ZJSX5003 CGMCC No.6480. The Trichoderma strain provided by the invention has the characteristics of high growth speed and high spore production amount; the in-vitro standoff pathogen inhibition rates are respectively up to 79.79%, 73.68% and 72.25%, the pathogen spore survival rates are respectively 0.00%, the in-vivo preventive effects for cucumber fusarium wilt and maize stem rot are respectively 85.08% and 66.67%, the enzyme activity of chitinase is 4.0783U, the enzyme activity of beta-1,3-glucanase is 0.8050U, the enzyme activity of extracellular proteinase is 4.8503U, and the content of silicone, terpene and carboxylic acid having antagonistic actions in antibiotic secondary metabolites is 44.44%. The Trichoderma strain provided by the invention has the advantages of high antagonism and high specificity. The biological pesticide produced from the strain can effectively control the soil-borne disease, thereby ensuring the stable increase of crop and vegetable yields.

The invention discloses lactobacillus rhamnosus LRa05, and the lactobacillus rhamnosus is collected in China General Microbiological Culture Collection Center, and the collection number is CGMCC 1.12734; the antagonistic ability of the lactobacillus rhamnosus has a good antagonistic effect on shigella, and the antagonistic ability of the lactobacillus rhamnosus is superior to that of common lactobacillus and commercial strain lactobacillus rhamnosus LGG; moreover, the lactobacillus rhamnosus has the characteristics of good acid resistance and bile tolerance; Animal test and human body test results show that a health-care food prepared from the lactobacillus rhamnosus LRa05 can effectively relieve symptoms including abdominal pain, diarrhea, fever and the like caused by the shigella; and besides, the lactobacillus rhamnosus belongs to probiotics, has been already incorporated into safe strains which can be applied to a food list directory, is excellent in safety, and can replace traditional antibiotics for use.

The invention discloses a special microorganism compound fertilizer for the reproduction and growth period of rice, and relates to a fertilizer which is made from the following materials: urea, ammonium sulfate, ammonium dihydrogen phosphate, potassium sulfate, magnesium sulfate, calcium nitrate, zinc chloride, copper chloride, manganese sulfate, ferric sulfate, ferrous sulfate, sodium molybdate, boric acid, decomposed farmyard manure, animal viscera leftovers, fermented rapeseed meal, corn straws, filler and a microbial agent. The special microorganism compound fertilizer can effectively improve the ratio of content of each element in the soil, can well fit with the nutrition demand law of the reproduction and growth period of the rice, and can ensure the growth quality of the rice in the reproduction and growth period, the material utilization rate of the fertilizer is enhanced, the rice grain quality is improved, and the special microorganism compound fertilizer has very good promotion use value.

The invention discloses a special biocontrol compound microbial fertilizer for grapes. An organic raw material is amino acid hydrolysis residue, and a bacterial agent is a mixed bacterial agent of bacillus subtilis and bacillus amyloliquefaciens; the strain in the bacterial agent is subjected to factory fermentation; the initial pH value of the culture medium is 6.5-7.5, and the fermentation temperature is 30-35 DEG C; and a nitrogen source comprises 140.0-106.0g/L corn flour, 0.6-1.3g/L glucoamylase, 1.2-1.8g/L alpha-amylase, 4.0-6.5g/L KH2PO4 and 3.0-6.0g/L CO(NH2)2. The invention also discloses a production method of the compound microbial fertilizer. In the special compound microbial fertilizer for grapes, disclosed by the invention, the microorganisms and the fertilizer enter the soil at the same time and are colonized at the root of a grape plant, and the growth of pathogenic bacteria of coniothyrium diplodiella is inhibited so as to reduce the morbidity of coniothyrium diplodiella.

The invention discloses medium-trace element fertilizer suitable for planting paddy rice in soil polluted by cadmium, which relates to fertilizer. The medium-trace element fertilizer is prepared from the following substances: calcium chloride, magnesium chloride, zinc sulfate, copper chloride, iron sulfate, ferrous sulfate, manganese chloride, borax, additives and filler. Not only can the medium-trace element fertilizer provide a great amount of medium trace elements to the paddy rice and improve the growth quality of the paddy rice, but also can play a role in well inhibiting the heavy metal cadmium; and when the medium-trace element fertilizer is applied to the paddy rice planted in the soil with severe pollution of heavy metal cadmium, the cadmium content in paddy rice grains can be reduced by a half or more, the application utilization effect is good, and the popularization value is high.

The invention discloses an antagonistic bacterium cooperating with AMF for disease resisting and growth promoting and application thereof. The antagonistic bacterium is bacillus amyloliquefaciens with an accession number of CGMCC No. 6491. According to the invention, bacterial strains with strong bacteriostatic activity are obtained by carrying out screening of the antagonistic bacterium on soil samples collected from a farmland cultivated for a plurality of years in a suburban area of Fukang, Xinjiang; then further screening and domestication are carried out, and the taxonomic status of obtained stains are preliminarily determined through determination of morphological characteristics and the 16 S rRNA sequence of the strains and phylogenetic analysis of the strains; moreover, in-depth research is carried out on characteristics like a bacteriostasis rate and stability of the fermentation broth of the bacterium. The antagonistic bacterium offers a new option for selection of antagonistic bacteria capable of controlling soil-borne diseases of vegetable; antagonistic action of the antagonistic bacterium exerts a prominent technical effect on treating tomato wilt, fusarium wilt of cucumber, phytophthora capsici leon and pepper anthracnose; and the antagonistic bacterium has wide application values.

The invention discloses a bacillus velezensis strain and application thereof. The invention specifically discloses a bacillus velezensis LPL-410.7 CGMCC (China General Microbiological Culture Collection Center) No.24303 strain and an application of the bacillus velezensis LPL-410.7 strain in prevention and treatment of plant diseases caused by phytopathogens and/or promotion of plant growth of the bacillus velezensis LPL-410.7 strain and the application of the bacillus velezensis LPL-410.7 strain. The bacillus velezensis LPL-410.7 disclosed by the invention has good organic phosphorus dissolving capacity and certain potassium dissolving capacity, and has relatively wide acid-base and salt tolerance; the strain can inhibit the growth of various pathogenic bacteria, generate hydrolase (protease and cellulase) related to pathogenic bacteria inhibition, generate siderophores and plant hormones (heteroauxin, gibberellin and cytokinin) for promoting plant growth, and is a biocontrol bacterium with excellent comprehensive performance. The method has good development and application prospects in the fields of agricultural disease control, soil improvement, microbial fertilizers and the like.

The invention relates to a bacilli, its compound preparation and the preparing method for the compound preparation. Its name is Bacillus subtilis LY-35, which is preserved in the general microbe center of Micro Germ conservation Management committee of China, and its conservation number is:CGMCC N0.1222. The preparation is a mixture of lambda phage of culture of bacilli and staphylococcus, streptococcus, colibacillus, bacillus proteus, pseudomonad, salmonella, pasteurella and klebsiella, can cure and prevent all kinds of illness caused by conditional pathogen,is a micro biological preparation which can be used by being mixed with antibiotics or replace antibiotics without any remainder and pollution, with good curative effect and safety.

The invention belongs to the technical field of environmental protection, and particularly discloses a method for treating soil cadmium-arsenic combined pollution by using a multifunctional manganese-based material. The method specifically comprises the steps: uniformly mixing the multifunctional manganese-based material with cultivated land soil; a preparation method of the multifunctional manganese-based material is prepared by the following steps: uniformly mixing a potassium permanganate aqueous solution with zeolite, and stirring to obtain a turbid liquid; heating the turbid liquid for reaction, drying to obtain a manganese-based material after the reaction is finished, and compounding the manganese-based material with limestone to obtain the multifunctional manganese-based material.According to the invention, permanganate is loaded on a zeolite mineral material, so that the oxidation and release rate of permanganate is reduced, and the long-term effect of permanganate in soil arsenic oxide and cadmium and arsenic immobilization is improved; Mn<2+> ions can also improve the antagonism of crops such as rice and the like on cadmium, and remarkably reduce the absorption of cropssuch as rice and the like on cadmium and the toxic action of cadmium on rice.

The invention discloses a microbial organic fertilizer, which is prepared from the following steps: inoculating Bacillus atrophaeus QHZ-3 having a preservation number of CGMCC No.13639 in a LB culture liquid, and carrying out liquid fermentation to obtain a QHZ-3 bacterial liquid having the effective viable count of more than or equal to 1*10<9> CFU/mL; preparing an amino acid organic fertilizer, wherein an animal source amino acid stock solution is added to an organic fertilizer according to a ratio of 15 ml/100 g, and fermenting is performed for 5 d to achieve the pH value of 6.5-6.8; and inoculating the QHZ-3 bacterial liquid in the amino acid organic fertilizer according to a ratio of 5 ml/100 g, and carrying out secondary solid fermentation, such that the effective viable count of the QHZ-3 is more than or equal to 0.2*10<9> cfu/g, and the water content is evaporated to less than 30%. According to the present invention, the microbial organic fertilizer has antagonistic effect on Rhizoctonia solani, and can effectively promote the growth of potato plants and increase the tuber yield.

The invention discloses a coniothyrium-minitans siderophore transporter (CmSit1) gene as well as a preparation method and application thereof. A coniothyrium-minitans insertion mutant library containing a plurality of T-DNA (transferred deoxyribonucleic acid) markers is obtained by adopting a mature agrobacterium-mediated genetic transformation method of the coniothyrium minitans. Transformants and sclerotinia sclerotiorum Ep-1PNA367 are cultured at the same time in the PDA (potato dextrose agar) culture medium, and as the comparison of a strain ZS-1, a strain ZS-1N1812 with enhanced anti-fungal capacity is selected. The further study shows that the expression of the CmSit1 gene can be improved significantly when T-DNA in the strain ZS-1N1812 is inserted into a promoter area of the CmSit1 gene, and the capacity of resisting the sclerotinia sclerotiorum of a plant can be improved significantly if the CmSit1 gene is transferred into the nicotiana benthamiana. The CmSit1gene can be used for improving the capacity of resisting pathogenic fungi of various biocontrol bacteria and can be also used for the transgenic breeding for disease resistance of crops.

The invention discloses a method for repairing cultivation soil by using a semi-matrix covering, and belongs to the field of agricultural planting. The method comprises the following steps that S1, surface soil of a cultivation tank is pretreated, wherein the soil 20-30 cm away from the ground is turned to obtain the pre-treated surface soil; S2, deep treatment of the surface soil is conducted, wherein a disinfectant is added to the filtered surface soil; S3, the cultivation tank is repaired and maintained, and the deep-treated surface soil is placed into the cultivation tank; S4, the semi-matrix covering is added, wherein the semi-matrix covering covers the surface of the cultivation tank; S5, data counting is performed, and repaired nutrient soil is obtained when the parameters of a germination test plant meet the normal plant standards. Through disinfectant application (a chemical modifier method), soil deep ploughing (a soil replacement method) and semi-matrix covering (a bioremediation method), multi-layer soil repairing is conducted, cultivation semi-soil semi-matrix repairing is conducted, the occurrence of disease and pest damage is reduced, the dead rate of plants is reduced and accordingly the annual yield is improved.

The invention belongs to the field of microbial biological control, and discloses streptomyces lilacinus Sl-3, the preservation number of which is CCTCC NO: M 2020423. The invention also discloses application of the streptomyces lilacinus Sl-3. The streptomyces lilacinus Sl-3 is used for preventing and treating plant diseases, and especially used for biological prevention and treatment of Xanthomonas campestris pv.

The invention belongs to the technical field of agricultural microorganisms and biology, and relates to a trichoderma viride engineering bacterium capable of synthesizing melatonine as well as a construction method and application of the trichoderma viride engineering bacterium. In order to solve the problem that synthesis of the melatonin is not found in a trichoderma strain at present, the invention constructs a trichoderma viride engineering bacterium with melatonin synthesis ability, the engineering bacterium heterologously expresses an aralkylamine-N-acetyltransferase (AANAT) coding gene hAANAT and an acetyl serotonin-O-methyltransferase (ASMT) coding gene hASMT which are derived from a human genome, and the starting strain is trichoderma viride Tv-1511. The genetically engineering bacterium constructed by the invention has the capability of synthesizing the melatonin, and the melatonin yield can be detected to reach 21.26 mg/L in fermentation liquor of the genetically engineering bacterium. Besides, the genetically engineering bacterium has better growth and sporulation characteristics, stronger adversity stress tolerance, higher pathogen antagonism and more effective plant growth promoting capability. The engineering bacterium can promote the application of the Trichodermaviride to agricultural and industrial production.

The invention relates to the field of biological prevention and treatment, in particular to an application of bacillus altitudinis Ba1449 and a fungicide in prevention and treatment of plant fungal diseases. The invention provides the application of the bacillus altitudinis Ba1449 in prevention and treatment of the plant fungal diseases, and the preservation number of the bacillus altitudinis Ba1449 is CGMCC No.22899. According to the application of the bacillus altitudinis Ba1449 and the fungicide in prevention and treatment of the plant fungal diseases, the bacillus altitudinis Ba1449 has high antagonistic ability on alternaria alternata brown spot bacteria and anthracnose bacteria at the same time, has no pollution to the environment, and has an important biocontrol application prospect.

The invention discloses a traditional Chinese medicine composition for treating acute and chronic viral hepatitis and a preparation method for the traditional Chinese medicine composition. The traditional Chinese medicine composition is prepared from 100-150g of Schisandra chinensis, 100-150g of Oldenlandia diffusa and 8-20g of Cordyceps sinensis. The traditional Chinese medicine composition has the effects of clearing heat and removing toxicity, supporting the healthy energy and simultaneously treating principal and subordinate symptoms, and is used for treating all kinds of acute and chronic viral hepatitis.

This invention describes a bacillus subtilis and the process for preparing its compound pharmaceutics. The bacillus subtilis is conserved at China General Microbiological Culture Collection Center of China Committee of Culture Collection for Microorganisms, the name is: Bacillus subtilis LY-35 and the conservation number is: CGMCC No. 1222. The pharmaceutics is a compound of the bacteriophages of such pathogens as bacillus subtilis, Staphylococcus, Streptocoaus, colibacillus, proteus, pseudomonas, salmonella, shigella, pasteurella and klebsiella. The pharmaceutics can prevent and treat the diseases caused by pathogens, and can be a non-polluted, high therapeutic effectiveness and safe substitute for antibiotics.