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Application of stilbene compounds in inhibiting pseudomonas aeruginosa quorum sensing system

A technology of Pseudomonas aeruginosa and quorum sensing system, applied in the field of medicine, can solve the problem that the QSI activity of Pseudomonas aeruginosa is not reported and the like

Inactive Publication Date: 2016-11-16
NANJING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on stilbene compounds focuses on their antibacterial, antioxidative and anticancer biological activities, but it has not been reported whether they have QSI activity against Pseudomonas aeruginosa

Method used

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  • Application of stilbene compounds in inhibiting pseudomonas aeruginosa quorum sensing system
  • Application of stilbene compounds in inhibiting pseudomonas aeruginosa quorum sensing system
  • Application of stilbene compounds in inhibiting pseudomonas aeruginosa quorum sensing system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Experiment of three stilbene compounds inhibiting pyocyanin production of Pseudomonas aeruginosa PAO1.

[0017] Prepare NB medium for sterilization and prepare for use; pick a single colony of Pseudomonas aeruginosa PAO1 that has been streaked and cultivated into NB medium, and cultivate overnight at 37°C; take 30 μL of overnight culture and dilute 1:1000 to contain 30 mL In the Erlenmeyer flask of NB medium, different concentrations of stilbene compounds resveratrol, piceatanol and oxidized resveratrol were added at the same time, so that the final concentrations were 100, 200, 300 and 400 μM respectively, and equal volumes of DMSO was used as the control group, mixed evenly and placed in a shaker, cultured at 150rpm, 37°C for 16-18h, each group was repeated three times; take 5mL of the culture solution obtained above into a 10mL centrifuge tube, and centrifuge at 8000×g for 5min; the supernatant Transfer the solution to a new 10mL centrifuge tube, add 3mL chloroform, ...

Embodiment 2

[0020] Determination of growth curves of three stilbene compounds.

[0021] Take 30 μL of the overnight cultured bacterial solution and dilute it 1:1000 into a conical flask containing 30 mL of NB medium. The final concentration was 400 μM, and an equal volume of DMSO was used as a control group, cultured at 150 rpm and 37°C; at the beginning of the initial culture, 200 μL of each culture solution was placed in a 96-well plate, and its absorbance at 620 nm was measured with a microplate reader; From the beginning of the culture, every 2 hours, the bacterial solution was taken to measure its absorbance at 620nm until 24 hours; the obtained data were analyzed statistically.

[0022] The reduction of pyocyanin production may be caused by the inhibition of the quorum sensing system of Pseudomonas aeruginosa by the compound, or by the inhibition of the growth of Pseudomonas aeruginosa by the compound. Therefore, to rule out the effect of growth inhibition on quorum sensing inhibit...

Embodiment 3

[0024] Experiment of three stilbene compounds inhibiting the swarming of Pseudomonas aeruginosa PAO1.

[0025] Prepare swarming medium (NB medium 8g, glucose 5g, agar 5g, distilled water 1000mL, adjust pH to about 7.2), after sterilization, add different concentrations of stilbene compounds resveratrol and alba Alcohol and oxidized resveratrol, mixed evenly and poured into a sterilized plate, in which the stilbene compounds resveratrol, picetanol and oxidized resveratrol were 100, 200, 300 and 400 μM, and at the same time An equal volume of DMSO was used as a solvent control. After the plate solidified, inoculate 2 μL of the overnight bacterial solution of PAO1 to the center of the agar plate; incubate upside down at 37°C for 16 hours and observe the results.

[0026] The swarming of Pseudomonas aeruginosa is regulated by its quorum sensing system. Therefore, the determination of the effects of the three compounds on the swarm movement of Pseudomonas aeruginosa can be used a...

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Abstract

The invention discloses an application of three stilbene compounds, namely resveratrol, piceatannol and oxyresveratrol, in inhibiting a pseudomonas aeruginosa quorum sensing system. Pseudomonas aeruginosa is one of the most common opportunistic pathogens which can cause serious acquired infection, and the quorum sensing system of the pseudomonas aeruginosa can regulate and control the generation of various virulence factors. In a sub-lethal concentration, the three stilbene compounds, namely the resveratrol, the piceatannol and the oxyresveratrol, can significantly inhibit the swarming of the pseudomonas aeruginosa and the generation of the virulence factor, namely pyocyanine, under the precondition of avoiding influence on the growth of the bacterium (the pseudomonas aeruginosa). The three stilbene compounds, namely the resveratrol, the piceatannol and the oxyresveratrol, can be prepared into pseudomonas aeruginosa quorum sensing inhibitors so as to effectively inhibit quorum sensing and prevent bacterium infection; and the inhibitors have an important practical value in clinical bacteriostatic application.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to the application of stilbene compounds in inhibiting Pseudomonas aeruginosa quorum sensing system, in particular to the application of three stilbene compounds, resveratrol, piceatanol and oxidized resveratrol, in inhibiting Pseudomonas aeruginosa Application of the quorum-sensing system of monocystic bacteria. Background technique [0002] The widespread use of antibiotics has resulted in more and more drug-resistant pathogenic bacteria. Therefore, it is urgent to develop new antibiotics and establish new treatment models. Recent studies have found that the pathogenicity of many pathogenic bacteria is regulated by a density-dependent quorum sensing system (Quorum Sensing, QS). The quorum sensing system regulates the formation of pathogenic bacteria biofilm by mediating the expression of pathogenic genes. , to regulate its pathogenicity. Efficient quorum sensing inhibitors (QSIs)...

Claims

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Application Information

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IPC IPC(8): A61K31/05A61P31/04
CPCA61K31/05
Inventor 贾爱群陈彤彤周金伟盛吉洋
Owner NANJING UNIV OF SCI & TECH
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