The invention relates to the technical field of detection kits, and particularly discloses an ELISA kit for quantitatively detecting tight junction related protein Occludin. The kit comprises an elisaplate coated with an Occludin coated antibody, HRP labeled goat anti-rabbit IgG, a rabbit anti-human Occludin polyclonal antibody, a washing solution, a sample diluent, a color developing agent, a stop solution, a quality control material and a standard substance, the kit is based on the Occludin coated antibody and the goat anti-rabbit IgG, and an ELISA method for Occludin protein detection is established by adopting a double antibody sandwiching method. The kit overcomes the defects in the prior art, can be used for effectively detecting the level of Occludin protein in a serum sample, is simple, convenient and rapid to operate, has good detection accuracy and precision, high specificity, good sensitivity and good stability, and can be used for rapidly and timely helping to diagnose theillness state and monitor prognosis.