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Lactobacillus plantarum for relieving ulcerative colitis and application thereof

A technology for ulcerative colitis and Lactobacillus plantarum, applied in the direction of Lactobacillus, application, bacteria used in food preparation, etc., to achieve the effects of increasing abundance, good tolerance, and increasing the content of short-chain fatty acids

Inactive Publication Date: 2021-07-23
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As the most common probiotics, a large number of studies have shown that some special Lactobacillus strains have good anti-inflammatory and intestinal flora effects, but this effect has obvious individual differences among Lactobacillus, and no better effect has been found so far. Good strain, can effectively anti-inflammation and repair the intestinal barrier

Method used

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  • Lactobacillus plantarum for relieving ulcerative colitis and application thereof
  • Lactobacillus plantarum for relieving ulcerative colitis and application thereof
  • Lactobacillus plantarum for relieving ulcerative colitis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1: Tolerance of Lactobacillus plantarum CCFM1117 to simulated gastrointestinal fluid

[0056] Inoculate the cryopreserved Lactobacillus plantarum CCFM1117 in mMRS medium (MRS medium + 0.05% cysteine ​​hydrochloride), culture anaerobically at 37°C for 48 hours, and subculture 2 to 3 times in mMRS medium Finally, take 1 mL of the culture solution of Lactobacillus plantarum CCFM1117, mix it with 9.0 mL of pH 2.5 artificial simulated gastric juice (containing 1% pepsin, mMRS medium at pH = 2.5), and culture it anaerobically at 37°C. Samples were taken at 0.5h, 1h, 2h, and 3h, and the mMRS agar medium was used to pour culture for plate colony counting, and the number of viable bacteria was determined and the survival rate was calculated (results are shown in Table 1).

[0057] Take 1 mL of the culture solution of Lactobacillus plantarum CCFM1117 and add it to 9 mL of artificial simulated intestinal fluid (mMRS medium containing 0.3% bovine bile salt, 1% trypsin, pH=8...

Embodiment 2

[0064] Example 2: Lactobacillus plantarum CCFM1117 has no toxic side effects on C57BL / 6J mice

[0065] The Lactobacillus plantarum CCFM1117 bacterium was resuspended in 3% (w / v) sucrose solution to make a concentration of 5.0×10 9 CFU / mL bacterial suspension. Get 6 healthy male C57BL / 6J mice with a body weight of about 14-16g. After acclimating to the environment for a week, give the bacterial suspension of this concentration once a day (each 0.2mL) orally, observe for a week, and record the death and body weight.

[0066] The results of these tests are listed in Table 3. These results indicated that feeding concentrations of 5.0×10 9 CFU / mL of Lactobacillus plantarum CCFM1117 had no significant impact on mice, no significant change in body weight, and no death. The appearance of the mice had no obvious pathological symptoms.

[0067] Table 3 Changes in body weight and death of mice

[0068]

[0069]

[0070] Note: -: no death of mice

Embodiment 3

[0071] Example 3: Application of Lactobacillus plantarum CCFM1117 in alleviating disease symptoms in UC mice

[0072] Take 30 healthy male C57BL / 6J mice weighing 14-16g, adapt to the environment for 1 week, 6 mice in each group, and randomly divide them into 5 groups: blank group, model group, drug group, Lactobacillus plantarum CCFM1117 intervention group ( CCFM1117), Lactobacillus plantarum QS6-12 control group (QS6-12). The dose of intragastric bacterial suspension was 5.0×10 9 CFU / mL, resuspended in 3% (w / v) sucrose solution. In the fifth week, dextran sodium sulfate (dextran sulphate sodium, DSS) with a final concentration of 2.5 g / 100 mL was added to drinking water for 7 days to induce colitis in mice.

[0073] The grouping and treatment methods of the experimental animals are shown in Table 4. Each time the mice were gavaged with the corresponding sucrose solution 0.2mL:

[0074] Table 4 Animal experiment design

[0075]

[0076] In the fifth week, during the mod...

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Abstract

The invention discloses a lactobacillus plantarum for relieving ulcerative colitis and application thereof, and belongs to the technical field of functional microorganisms. The lactobacillus plantarum CCFM1117 can tolerate the gastrointestinal environment of a human body, reduce weight loss in the ulcerative colitis disease period, improve fecal characters and hemafecia conditions, relieve length shortening of the colon, improve colonic mucosal injury, reduce the content of proinflammatory factors TNF-alpha, IL-1beta, IL-6 and IFN-gamma in the colon, up-regulate the gene transcription level of colon tight junction related proteins Claudin-3, ZO-1, ZO-2 and Occludin, increase the content of short-chain fatty acid, increase the abundance of short-chain fatty acid producing bacteria Coprococcus and butyric acid producing bacteria Faecalibacterium, and improve the diversity of intestinal flora.

Description

technical field [0001] The invention relates to a strain of Lactobacillus plantarum for alleviating ulcerative colitis and its application, in particular to the application of Lactobacillus plantarum in the preparation of functional bacterial agents, food and / or medicine, and belongs to the technical field of functional microorganisms. Background technique [0002] Ulcerative colitis (UC) is a kind of inflammatory bowel disease (Inflammatory bowel disease, IBD), the main clinical manifestations are chronic or subacute diarrhea, mucus pus and blood in the stool and abdominal pain. UC originated in western developed countries, and its incidence and prevalence have been on the rise worldwide in recent years, and it has become a global disease. UC is a continuous inflammation of the colonic mucosa and submucosa, and the disease usually starts from the rectum and spreads to the whole colon. UC has a long course of disease and is prone to recurrence, which not only affects the qu...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L33/135A23L29/00A23L11/50A23C9/123A23C13/16A23C19/00A61K35/747A61P1/00A61P1/04C12R1/25
CPCA23L33/135A23L29/065A23C9/1234A23C13/16A23C19/00A61K35/747A61P1/00A61P1/04A23V2002/00A23V2400/169A23V2200/3204
Inventor 赵建新王刚郑雨星王琳琳翟齐啸陆文伟崔树茂杨波毛丙永唐鑫张灏陈卫
Owner JIANGNAN UNIV
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