84 results about "Colony counting" patented technology

The invention discloses a solid fermentation production method of bacillus coagulans. The method comprises the steps of: using bacillus coagulans as fermentation strain; culturing the bacillus coagulans in an optimized solid culture medium at 37-40 DEG C; performing large-scale fermentation production by three-stage amplification; performing colony counting on the bacillus coagulans culture, wherein the concentration of viable bacteria is as high as 9.10*10<9>CFU/g fresh yeast, and the concentration of spore is as high as 2.45*10<9>CFU/g fresh yeast. The method disclosed by the invention has the beneficial effects that the production technique is simple and stable; the viable bacteria has high density; the fermentation products can be directly used without separation and purification, and are easy to produce and popularize; meanwhile, bacillus coagulans is added into feed as a feed additive and can be planted in animal caecum, colon and rectum so as to produce a large quantity of anti-microbial coagulin, lactic acid, amino acid and vitamins and various digestive enzymes by fermentation, so that the quality of the feed is improved, digestion and adsorption of the feed are promoted, and the feed conversion ratio is lowered.

The invention discloses a non-destructive inspection method of total amount of meat bacteria, comprising the following steps: S1, obtaining an original reflection spectrum image Rs of a sample to be detected and storing the image; S2, detecting the TVC of the sample as standard reference data with a standard plate colony counting method according to National Standard; S3, obtaining a relative reflection spectrum image R of the sample according to the FORMULA that R=(Rs-Rd)/(Rr-Rd), wherein Rd is a black image when an image obtaining system works with dark current, and Rr is a reflection spectrum image of a standard reference whiteboard; S4, correcting the image with an SNV method; S5, choosing a second-order differential spectrum for the image R after being corrected, and selecting the best wavelength representing the TVC of the sample to be detected with a step-by-step regression method; S6, extracting the data and TVC detection result of the second-order differential spectrum relative to the best wavelength, and dividing the data and TVC detection result into two sets respectively as correction set data and validation set data; S7, establishing an LS-SVM data model, and detecting the value of the TVC of the sample to be detected. The method is human-oriented and advanced with quick and pollution-free detection process so that our country is in line with developed countries in the field of food quality and safety.

The invention discloses a detection method of staphylococcus aureus in milk through a double signal amplification effect and belongs to the technical field of biosensing. According to the technology,a gold-platinum nano composite material and DNA mimic enzyme are used for synthesizing nano probes which have the good double catalytic effect on H2O2, a modified electrode made of a graphene/gold nanoparticle nano composite material is used so that the electron transfer capacity of the modified electrode can be improved, and accordingly quick high-sensitivity detection for staphylococcus aureus is achieved. Under the optimized experimental conditions, an electrochemical immunosensor has a wide linear detection range for staphylococcus aureus. In addition, the sensor is successfully applied toquantitative detection of staphylococcus aureus in pure milk and yogurt samples. Compared with traditional methods such as a slab colony counting method, the detection method is convenient and fast,easy to operate and high in sensitivity, and quick high-sensitivity detection for staphylococcus aureus is achieved.

Provided is an intelligent bacterium colony counting method. An image collection module, an edge filtering module, a binary processing module, an image segmentation module, a characteristic matching module, a result display module and a parameter interaction setting module are included; the image collection module reads in an image of bacterium colonies to be counted; the edge filtering module determines a distribution area of the counting bacterium colonies, and sets points beyond the area as a background; the binary processing module converts an image in the counting area into a binary image; the image segmentation module divides the image in the counting area into independent image blocks, and each block includes only one bacterium colony; the characteristic matching module implements characteristic matching according to a bacterium colony image selected by a user and each image block obtained by segmentation, and an image which succeeds in matching is a bacterium colony image; the result display module displays a matching result, and adjusts the matching result according to selection of the user; and the parameter interaction setting module interacts with the user, reminds the user of input, and receives the result input by the user.

An imaging system and method for microbial growth detection, counting or identification. One colony may be contrasted in an image that is not optimal for another type of colony. The system and method provides contrast from all available material through space (spatial differences), time (differences appearing over time for a given capture condition) and color space transformation using image input information over time to assess whether microbial growth has occurred for a given sample.

The invention discloses a method for detecting external influences of tobacco chewing gum on oral microorganisms of human. The method comprises the following steps: (1) the tobacco chewing gum to be detected is frozen in liquid nitrogen; (2) the tobacco chewing gum is pulverized by a pulverizer; (3) pulverized tobacco chewing gum is frozen again; (4) the pulverized tobacco chewing gum is immersed in artificial saliva; (5) centrifugation is carried out for an extract; (6) an osmotic pressure tester is used for determination; (7) the content of nicotine in the extract is determined; (8) in vitro culture is carried out for oral microorganisms of human; (9) the extract of the tobacco chewing gum is added into cultivated oral microorganisms of human; (10) growth condition is checked, and colony counting is carried out.

The invention relates to a total bacterial count test piece and a manufacturing method thereof. The total bacterial count test piece comprises a carrier structure and further includes two layers of gaskets, an upper layer base material and a lower layer base material. The carrier structure is formed by combining a nonwoven and a filter film. The total bacterial count test piece can contain 500 [mu]l of liquid. The total bacterial count test piece manufactured through the manufacturing method can count red bacterial colonies after a liquid sample is inoculated and cultivation is conducted under the condition of 37 DEG C for 48 h. Through the contrast experiment result of a test piece method and a national standard method, the effects can be known that the experimental bacteria detection conformity of the test piece can reach 105%, and the practical sample total bacterial count detection conformity of the test piece can reach 89% or above. Bacterial colony counting is conducted through the test piece, operation is simple and convenient, bacterial colony forms are good, and results are accurate.

The embodiment of the invention provides a bacterial colony counting method and device and a computer storage medium. The bacterial colony counting method comprises the following steps: performing noise reduction processing and edge feature extraction processing on an acquired bacterial colony image to obtain a bacterial colony binary image containing bacterial colony edge information and bacterial colony feature information in the bacterial colony image; carrying out distance transformation processing on the bacterial colony binarization image to convert the bacterial colony binarization image into a bacterial colony gray level image, wherein the gray level value of the bacterial colony edge indicated by bacterial colony edge information in the bacterial colony gray level image is smaller than the gray level value of the interior of the bacterial colony indicated by bacterial colony feature information; according to the gray value in the bacterial colony gray image, carrying out bacterial colony segmentation on the bacterial colony gray image by using a watershed algorithm, and obtaining segmented bacterial colony information; carrying out connected domain analysis on the bacterial colony binarization image according to the segmented bacterial colony information; and determining a bacterial colony numerical value in the bacterial colony image according to a connected domain analysis result. The method is more accurate in counting.

A device and a process to count a number of colonies present in a set of samples. The colony counting device has a storage device with plural storage locations. A handling system is operative to convey samples to analyze from storage locations to an analysis area and from the analysis area to storage locations. An imaging device is operative to acquire a plurality of images of a sample to analyze, the plurality of images including all or a part of the analysis area. A processing unit is operative to implement, for each sample to analyze, a detection step to detect the presence of colonies by analyzing an image of the plurality of images of the sample and a determination step to determine the number of colonies present in the sample by counting the colonies whose presence have been detected during the detection step.

The invention provides a method for measuring the total number of fermented milk living bacteria. The method comprises the steps of adding 2g/L of ethylene diamine tetraacetic acid (EDTA) solution with the pre-dilution volume fraction of 2-6% into a fermented milk sample; diluting the fermented milk sample, and adjusting the pH value to be 6.8-8.8; and carrying out colony counting, and calculating the total number of the fermented milk living bacteria. The method enables fermented milk diluent to become clear within a short time and bacterial cells to be evenly dispersed, and the step of oscillating the sample diluent for about 20 minutes is omitted in a measuring process; the accuracy of a measurement result of the method is higher than that of the measurement result of GB4789.35-2010.

The invention discloses a detection device and method for colony growth states based on the multi-mode imaging technology. The device includes a white light source, a coherent laser source and a fluorescent excitation light source, which are used as three kinds of light sources to irradiate the colony samples above the slope for sample image acquisition; a color camera, a high-speed camera and a cooling camera, which are used as three kinds of cameras to collect the images of colony samples irradiated by three kinds of light sources above the sample respectively; an imaging system, including imaging lens, a spectroscope, a filter and a relay eyepiece, which is set between the colony samples and the cameras to realize the corresponding imaging between the colony samples irradiated by threekinds of light sources and the three kinds of cameras, and the imaging parameters are identical; and a computer, through which the imaging signals are received to complete image fusion processing. Multimodal optical imaging and information fusion method are used to eliminate the background effect of culture medium, improving the accuracy of colony counting, and realizing all-round detection and discrimination of colony growth status.

The invention discloses a multifunctional and efficient bacterial detector for a public place. The multifunctional and efficient bacterial detector comprises a box, wherein a liquid culture medium storage device is arranged on one side of the upper side of the interior of the box, a flushing device is arranged on the other side of the upper side of the interior of the box, a bacterial culture detection device is arranged on the lower side of the interior of the box, by use of an adjustable culture dish in the box, the culture dish can culture bacteria and be flushed, so that the culture dish can be reused, external air enters the box to make contact with the culture medium to culture bacteria, the number of bacteria can be detected by a bacterial colony counter, and the content of the bacteria in the air can be detected regularly. The multifunctional and efficient bacterial detector has the beneficial effects of simple structure and high practicability.

The invention discloses applications of a lectin-modified anchovy antibacterial peptide liposome in inhibition of Listeria monocytogenes and a biofilm thereof, and belongs to the technical field of food microorganism control. According to the present invention, the lectin-modified anchovy antibacterial peptide liposome is prepared according to the specific affinity between the lectin and the saccharide unit in the capsule extracellular polysaccharide matrix, the Listeria monocytogenes biofilm model is established, and the inhibition effect of the lectin-modified anchovy antibacterial peptide liposome on the Listeria monocytogenes and the biofilm thereof is evaluated through the sterilization curve, the colony counting and the crystal violet staining, wherein the results show that the lectin-modified anchovy antibacterial peptide liposome provides the sterilization effect on the Listeria monocytogenes, can interfere the formation of the biofilm, and can eliminate the formed biofilm, and the effect is concentration dependent, such that the lectin-modified anchovy antibacterial peptide liposome can be used for inhibiting or clearing the Listeria monocytogenes and the biofilm thereof in the food processing and storage environments.

The invention relates to a microbial colony counting instrument which comprises a sample layer, an amplification layer and a shooting layer which are sequentially arranged from bottom to top, and further comprises a first light shield; the amplification layer comprises a mounting plate, and a plurality of microscope assemblies are arranged on the mounting plate; the shooting layer comprises a shooting camera, a shooting turntable and a rotating assembly; the sample layer comprises a plurality of sample index plates, and a plurality of sample grooves are annularly distributed in the sample index plates; and a second light shield is arranged in the first light shield, and the second light shield is longitudinally and slidably connected to the inner top of the first light shield. By means of the method, batch automatic detection can be conducted after one-time feeding, external light interference is reduced to the minimum through the double light shields, and the peripheral light shields are used for shielding light and serve as supporting brackets of the overall structure; and meanwhile, only one shooting camera is needed, the overall structure is reasonable and compact, the detection efficiency is high, the automation degree is high, and the cost is low.

The invention provides a bacterial colony counting method based on machine vision in the field of bacterial colony counting, which comprises the following steps: step S10, acquiring an original photoof a culture dish, and preprocessing the original photo to generate an optimized photo; S20, traversing and searching a first outline of a culture dish in the optimized photo, setting an area outsidethe first outline to be black, and generating a photograph of the culture dish; S30, traversing and searching a second outline of each bacterial colony in the photograph; and step S40, counting the second contours. The bacterial colony counting device has the advantage that the bacterial colony counting efficiency and precision are greatly improved.

The present invention relates to a method for counting lactic acid bacteria, bacillus and yeast in a fermented feed. The method is as below: diluted fermented feed samples are separately counted in anMRS solid medium containing natamycin, a YPD medium containing streptomycin and penicillin, and an LB medium, so that the number of lactic acid bacteria, bacillus and yeast in the fermented feed canbe more accurately counted, and the counting accuracy of single microorganisms in the fermented feed is improved; and the method is easy to operate.

The invention relates to an intelligent colony counting incubator and a counting method. The intelligent colony counting incubator comprises a computer assembly, an imaging assembly, a mechanical arm,a culture utensil station, a heating pipe, a recovery assembly, a horizontal bottom plate, a box inlet assembly and a door opening and closing assembly, wherein the computer assembly is in sliding connection with a stand column; the imaging assembly is fixedly connected to the bottom plate in the incubator; the mechanical arm is fixedly connected with the horizontal bottom plate; the culture utensil station is semi-annular, and is fixedly connected with the horizontal bottom plate; the recovery assembly consists of a box outlet part and a recovery part, wherein the box outlet part is fixedlyconnected to the horizontal bottom plate; the recovery part is fixedly connected to the outer side of the box body; the box inlet assembly is fixedly connected with the horizontal bottom plate; and the door opening and closing assembly is fixedly connected to the front inner wall surface of the box body. The intelligent colony counting incubator disclosed by the invention is simple in mechanical structure and reasonable in layout, automation of colony culture, colony imaging counting and colony recovery can be realized, labor is saved, efficiency is improved, cost is reduced, and the intelligent colony counting incubator has important significance in development of colony culture.

The invention provides an induction method of a VBNC state of lactobacillus casei Zhang and a detection method of cell membrane fatty acid in the VBNC state, and belongs to the technical field of microbial culture. The induction method comprises the following steps: inoculating the lactobacillus casei Zhang into a liquid MRS culture medium for activation, and then inoculating the lactobacillus casei Zhang into an MRS induction solution for induction in the VBNC state at 3.5-4.5 DEG C until the number of living cells counted by a plate colony counting method is 0 and the number of living cellscounted by a fluorescence microscope method is greater than 0 so as to obtain the VBNC state of the lactobacillus casei Zhang. By the method, cells in the VBNC state are successfully obtained, and thetypes of the VBNC state induced induction solution of the lactobacillus casei Zhang are enriched. The invention also provides a difference analysis result for extracting the components and contents of the membrane fatty acid in the normal state and the VBNC state of the lactobacillus casei Zhang.

The invention discloses a Bacillus spp culture medium with coconut water as a main raw material. The culture medium comprises: (1) a growth stimulation culture medium (/L); (2) a liquid amplification culture medium (/L); and (3) a colony counting culture medium (/L). According to the invention, economical, cheap and widely available coconut water is used as the main raw material for the Bacillus spp culture medium, and a scientific and reasonable application method of the Bacillus spp culture medium is constructed at the same time; with the application method, the culture medium can meet requirements of scale-up culture of Bacillus spp; a leftover of coconut processing in Hainan province, i.e., coconut water, is made full use of, the Bacillus spp culture medium with coconut water as the main raw material is created for the first time, a good cheap culture medium formula is provided for development of scale-up culture of Bacillus spp in the future, and the Bacillus spp culture medium has a good application prospect; an effective approach is provided for high-valued utilization of coconut water, an added value of coconut processing can be obviously increased, and pollution of the industry of coconut processing to the environment can be reduced at the same time.