84 results about "Colony counting" patented technology

The invention discloses a non-destructive inspection method of total amount of meat bacteria, comprising the following steps: S1, obtaining an original reflection spectrum image Rs of a sample to be detected and storing the image; S2, detecting the TVC of the sample as standard reference data with a standard plate colony counting method according to National Standard; S3, obtaining a relative reflection spectrum image R of the sample according to the FORMULA that R=(Rs-Rd) / (Rr-Rd), wherein Rd is a black image when an image obtaining system works with dark current, and Rr is a reflection spectrum image of a standard reference whiteboard; S4, correcting the image with an SNV method; S5, choosing a second-order differential spectrum for the image R after being corrected, and selecting the best wavelength representing the TVC of the sample to be detected with a step-by-step regression method; S6, extracting the data and TVC detection result of the second-order differential spectrum relative to the best wavelength, and dividing the data and TVC detection result into two sets respectively as correction set data and validation set data; S7, establishing an LS-SVM data model, and detecting the value of the TVC of the sample to be detected. The method is human-oriented and advanced with quick and pollution-free detection process so that our country is in line with developed countries in the field of food quality and safety.

The invention discloses a detection method of staphylococcus aureus in milk through a double signal amplification effect and belongs to the technical field of biosensing. According to the technology,a gold-platinum nano composite material and DNA mimic enzyme are used for synthesizing nano probes which have the good double catalytic effect on H2O2, a modified electrode made of a graphene / gold nanoparticle nano composite material is used so that the electron transfer capacity of the modified electrode can be improved, and accordingly quick high-sensitivity detection for staphylococcus aureus is achieved. Under the optimized experimental conditions, an electrochemical immunosensor has a wide linear detection range for staphylococcus aureus. In addition, the sensor is successfully applied toquantitative detection of staphylococcus aureus in pure milk and yogurt samples. Compared with traditional methods such as a slab colony counting method, the detection method is convenient and fast,easy to operate and high in sensitivity, and quick high-sensitivity detection for staphylococcus aureus is achieved.

Provided is an intelligent bacterium colony counting method. An image collection module, an edge filtering module, a binary processing module, an image segmentation module, a characteristic matching module, a result display module and a parameter interaction setting module are included; the image collection module reads in an image of bacterium colonies to be counted; the edge filtering module determines a distribution area of the counting bacterium colonies, and sets points beyond the area as a background; the binary processing module converts an image in the counting area into a binary image; the image segmentation module divides the image in the counting area into independent image blocks, and each block includes only one bacterium colony; the characteristic matching module implements characteristic matching according to a bacterium colony image selected by a user and each image block obtained by segmentation, and an image which succeeds in matching is a bacterium colony image; the result display module displays a matching result, and adjusts the matching result according to selection of the user; and the parameter interaction setting module interacts with the user, reminds the user of input, and receives the result input by the user.

The invention discloses quantitative determination and drug allergy determination kits for helicobacter pylori viable bacteria and a determination method. The method adopts viable helicobacter pylori as a sample to be detected; the property that the viable helicobacter pylori can generate urease is used for rapidly and accurately reading the quantity of the helicobacter pylori by a helicobacter pylori colony counting standard method (CFU / ml, namely the bacterium individual quantity in each ml of bacterium liquid and a colony quantity standard curve); the detection result is accurate and sensitive and has high reliability; the counting difficulty of existing helicobacter pylori clinic microbiological identification, caused by difficult culture and complicated operation, is solved; the drug allergy determination kit and a preparation method, which are expanded by the invention, can be used for carrying out various drug allergy tests at the same time; clinicians can rapidly and conveniently screen suitable anti-helicobacter pylori medicines so that the time and the labor are saved and the cost is saved, so as to provide a beneficial technical solution for clinical scientific treatment.

An imaging system and method for microbial growth detection, counting or identification. One colony may be contrasted in an image that is not optimal for another type of colony. The system and method provides contrast from all available material through space (spatial differences), time (differences appearing over time for a given capture condition) and color space transformation using image input information over time to assess whether microbial growth has occurred for a given sample.

The invention discloses a solid culture medium for quick detection of mycoplasma, and a preparation method thereof. By using the solid culture medium disclosed by the invention, the existence of ureaplasma urealyticum and mycoplasma hominis can be accurately detected, and the content of mycoplasmas can be quantitatively concluded by colony counting, thus greatly improving the accuracy and reliability. Simultaneously, by using the culture medium disclosed by the invention for detecting mycoplasmas, the result can be read after only 18 hours, thereby realizing quick detection of mycoplasmas, and finally confirming diagnosis of urogenital tract infection caused by ureaplasma urealyticum and mycoplasma hominis.

The invention discloses a method for detecting external influences of tobacco chewing gum on oral microorganisms of human. The method comprises the following steps: (1) the tobacco chewing gum to be detected is frozen in liquid nitrogen; (2) the tobacco chewing gum is pulverized by a pulverizer; (3) pulverized tobacco chewing gum is frozen again; (4) the pulverized tobacco chewing gum is immersed in artificial saliva; (5) centrifugation is carried out for an extract; (6) an osmotic pressure tester is used for determination; (7) the content of nicotine in the extract is determined; (8) in vitro culture is carried out for oral microorganisms of human; (9) the extract of the tobacco chewing gum is added into cultivated oral microorganisms of human; (10) growth condition is checked, and colony counting is carried out.

The invention relates to a total bacterial count test piece and a manufacturing method thereof. The total bacterial count test piece comprises a carrier structure and further includes two layers of gaskets, an upper layer base material and a lower layer base material. The carrier structure is formed by combining a nonwoven and a filter film. The total bacterial count test piece can contain 500 [mu]l of liquid. The total bacterial count test piece manufactured through the manufacturing method can count red bacterial colonies after a liquid sample is inoculated and cultivation is conducted under the condition of 37 DEG C for 48 h. Through the contrast experiment result of a test piece method and a national standard method, the effects can be known that the experimental bacteria detection conformity of the test piece can reach 105%, and the practical sample total bacterial count detection conformity of the test piece can reach 89% or above. Bacterial colony counting is conducted through the test piece, operation is simple and convenient, bacterial colony forms are good, and results are accurate.

The embodiment of the invention provides a bacterial colony counting method and device and a computer storage medium. The bacterial colony counting method comprises the following steps: performing noise reduction processing and edge feature extraction processing on an acquired bacterial colony image to obtain a bacterial colony binary image containing bacterial colony edge information and bacterial colony feature information in the bacterial colony image; carrying out distance transformation processing on the bacterial colony binarization image to convert the bacterial colony binarization image into a bacterial colony gray level image, wherein the gray level value of the bacterial colony edge indicated by bacterial colony edge information in the bacterial colony gray level image is smaller than the gray level value of the interior of the bacterial colony indicated by bacterial colony feature information; according to the gray value in the bacterial colony gray image, carrying out bacterial colony segmentation on the bacterial colony gray image by using a watershed algorithm, and obtaining segmented bacterial colony information; carrying out connected domain analysis on the bacterial colony binarization image according to the segmented bacterial colony information; and determining a bacterial colony numerical value in the bacterial colony image according to a connected domain analysis result. The method is more accurate in counting.

A device and a process to count a number of colonies present in a set of samples. The colony counting device has a storage device with plural storage locations. A handling system is operative to convey samples to analyze from storage locations to an analysis area and from the analysis area to storage locations. An imaging device is operative to acquire a plurality of images of a sample to analyze, the plurality of images including all or a part of the analysis area. A processing unit is operative to implement, for each sample to analyze, a detection step to detect the presence of colonies by analyzing an image of the plurality of images of the sample and a determination step to determine the number of colonies present in the sample by counting the colonies whose presence have been detected during the detection step.

The invention provides a method for measuring the total number of fermented milk living bacteria. The method comprises the steps of adding 2g / L of ethylene diamine tetraacetic acid (EDTA) solution with the pre-dilution volume fraction of 2-6% into a fermented milk sample; diluting the fermented milk sample, and adjusting the pH value to be 6.8-8.8; and carrying out colony counting, and calculating the total number of the fermented milk living bacteria. The method enables fermented milk diluent to become clear within a short time and bacterial cells to be evenly dispersed, and the step of oscillating the sample diluent for about 20 minutes is omitted in a measuring process; the accuracy of a measurement result of the method is higher than that of the measurement result of GB4789.35-2010.

The invention discloses a detection device and method for colony growth states based on the multi-mode imaging technology. The device includes a white light source, a coherent laser source and a fluorescent excitation light source, which are used as three kinds of light sources to irradiate the colony samples above the slope for sample image acquisition; a color camera, a high-speed camera and a cooling camera, which are used as three kinds of cameras to collect the images of colony samples irradiated by three kinds of light sources above the sample respectively; an imaging system, including imaging lens, a spectroscope, a filter and a relay eyepiece, which is set between the colony samples and the cameras to realize the corresponding imaging between the colony samples irradiated by threekinds of light sources and the three kinds of cameras, and the imaging parameters are identical; and a computer, through which the imaging signals are received to complete image fusion processing. Multimodal optical imaging and information fusion method are used to eliminate the background effect of culture medium, improving the accuracy of colony counting, and realizing all-round detection and discrimination of colony growth status.

The present invention provides a detection method for the Psychrophile content of milk and dairy products. According to the method, the culture conditions after inoculation are as follows: first incubating at 16-18 DEG C for 18-20 hours, and then incubating at 6-7 DEG C for 4 days. The detection method of the invention costs little time in detection; and the bacterial colonies finally formed is relatively large so errors are not easy to generate during counting. The detection results of the detection method of the invention have a good correlation with the detection results of a 6.5 DEG C colony counting method.

The invention discloses a multifunctional and efficient bacterial detector for a public place. The multifunctional and efficient bacterial detector comprises a box, wherein a liquid culture medium storage device is arranged on one side of the upper side of the interior of the box, a flushing device is arranged on the other side of the upper side of the interior of the box, a bacterial culture detection device is arranged on the lower side of the interior of the box, by use of an adjustable culture dish in the box, the culture dish can culture bacteria and be flushed, so that the culture dish can be reused, external air enters the box to make contact with the culture medium to culture bacteria, the number of bacteria can be detected by a bacterial colony counter, and the content of the bacteria in the air can be detected regularly. The multifunctional and efficient bacterial detector has the beneficial effects of simple structure and high practicability.

The invention discloses applications of a lectin-modified anchovy antibacterial peptide liposome in inhibition of Listeria monocytogenes and a biofilm thereof, and belongs to the technical field of food microorganism control. According to the present invention, the lectin-modified anchovy antibacterial peptide liposome is prepared according to the specific affinity between the lectin and the saccharide unit in the capsule extracellular polysaccharide matrix, the Listeria monocytogenes biofilm model is established, and the inhibition effect of the lectin-modified anchovy antibacterial peptide liposome on the Listeria monocytogenes and the biofilm thereof is evaluated through the sterilization curve, the colony counting and the crystal violet staining, wherein the results show that the lectin-modified anchovy antibacterial peptide liposome provides the sterilization effect on the Listeria monocytogenes, can interfere the formation of the biofilm, and can eliminate the formed biofilm, and the effect is concentration dependent, such that the lectin-modified anchovy antibacterial peptide liposome can be used for inhibiting or clearing the Listeria monocytogenes and the biofilm thereof in the food processing and storage environments.

The invention relates to a microbial sensor based on turbidity detection and a turbidity detection method. The microbial sensor based on the turbidity detection comprises a light source, a first light guiding device, a second light guiding device, a detector and a container used for placing an object to be detected, wherein exciting light emitted by the light source passes through the first light guiding device, then passes through the object to be detected, is received by the second light guiding device and finally is transmitted to enter the detector. The microbial sensor provided by the invention has the beneficial effects that compared with 90-degree scattered light detection in the traditional turbidity detection, two side-by-side light guiding devices are adopted for detecting scattered light, and the characteristics of concentrated light intensity, high receiving sensitivity and strong anti-jamming capability are realized; compared with the traditional colony counting method, luminous power values received by the light guiding devices are of comprehensive response on all the microorganisms in light spot areas of the exciting light, so that the advantage of rapid detection is realized; and compared with other rapid detection methods, a colony is directly detected, thereby being more intuitive and reliable.

The invention relates to the technical field of biological detection, in particular to a culture medium for detecting microorganisms in human blood and a preparing method for the culture medium. The culture medium for detecting microorganisms in human blood comprises a basic culture medium and additives; the additives comprise the following substances of, by mass, 5-15 parts of peptone, 2.5-7.5 parts of sodium chloride, 1-3 parts of glucose, 0.5-4 parts of lactose, 1.5-3.5 parts of phosphorus source, 0.01-0.08 part of bile salt pig, 0.5-6 parts of mannitol, 1-3 parts of sodium pyruvate, 1-6 parts of amino acid, 1-8 parts of beta-nuclear glycosidase substrate, 1-3 parts of mango extract, 0.02-0.58 part of metal salt, 0.01-0.05 part of surfactant, 7.2-7.5 of pH value, and 1000 parts of distilled water. According to the culture medium for detecting microorganisms in human blood, the true content of different microorganisms in blood can be directly inferred by colony counting and using solid culture medium, whether the blood is in a carrier state or has reached the pathogenic concentration can be determined from the quantitative point of view, so that veracity of detecting results is further improved.

The invention discloses a microbial plate culture method. The microbial plate culture method comprises the steps of plate culture medium preparation, active sludge mixed liquor preparation, culture medium coating, bacterial colony culture and bacterial colony separation. The microbial plate culture method is easy to operate, has no requirement on colonial morphology of bacteria, and is convenient for observing colony characteristics and colony counting; by adopting the method, the requirement on the plate culture medium is lowered, and production and scientific research work are ensured.

The invention discloses a bacterial colony height scanning and imaging device which comprises a supporting frame, a data collector, a bacterial colony bearing mechanism, an auxiliary light source assembly and a controller, the data collector is installed in the supporting frame, and the data collector comprises a camera and a miniature laser distance measuring sensor. According to the invention, bacterial colony two-dimensional image acquisition and bacterial colony three-dimensional image acquisition containing height information can be realized at the same time, bacterial colony image information with richer content is provided for bacterial colony counting and identification, and the accuracy of automatic bacterial colony counting equipment is improved. The invention also discloses a bacterial colony automatic counting device and method capable of distinguishing atypical bacterial colonies, and the method comprises the steps: firstly, carrying out the preliminary counting of bacterial colonies based on a bacterial colony two-dimensional image in combination with a plurality of image processing technologies; checking the preliminary colony counting result based on the colony three-dimensional image; and high accuracy of a bacterial colony counting result is ensured from multiple angles. The artificial intelligence classification technology is further applied to the bacterial colony counting process, and atypical bacterial colony distinguishing is achieved.

The invention relates to the technical field of colony counting, in particular to a bacteria counting image acquisition device which comprises a projection component and a closed component which are connected in sequence; the closed component comprises a first shell and a first inner cylinder, and the first shell can rotate relative to the first inner cylinder; the first shell is provided with a first opening part; the first inner cylinder is provided with a second opening part; when the first inner cylinder rotates to a preset position, the first opening part coincides with the second openingpart and is used for containing a bacteria box into the first inner cylinder; and when the first inner cylinder deviates from the preset position, a closed space is formed in the first inner cylinder. According to the bacteria counting image acquisition device provided by the invention, the bacteria box is contained in the closed space, so that light loss is avoided, the illumination intensity isensured, an image formed by irradiating light to bacterial colonies can be clearly displayed, the loss of the bacterial colonies in the formed image is avoided, and the accuracy of a result of counting the bacterial colonies in the later period is ensured.

The invention relates to a microbial colony counting instrument which comprises a sample layer, an amplification layer and a shooting layer which are sequentially arranged from bottom to top, and further comprises a first light shield; the amplification layer comprises a mounting plate, and a plurality of microscope assemblies are arranged on the mounting plate; the shooting layer comprises a shooting camera, a shooting turntable and a rotating assembly; the sample layer comprises a plurality of sample index plates, and a plurality of sample grooves are annularly distributed in the sample index plates; and a second light shield is arranged in the first light shield, and the second light shield is longitudinally and slidably connected to the inner top of the first light shield. By means of the method, batch automatic detection can be conducted after one-time feeding, external light interference is reduced to the minimum through the double light shields, and the peripheral light shields are used for shielding light and serve as supporting brackets of the overall structure; and meanwhile, only one shooting camera is needed, the overall structure is reasonable and compact, the detection efficiency is high, the automation degree is high, and the cost is low.

The invention discloses a high-flux multi-species bacterial colony counting method which comprises the following steps: in-situ timing detection: turning on a background light source, aligning a plurality of cameras with a culture dish to be observed, performing in-situ timing detection on the plurality of culture dishes to be observed at the same time, and acquiring and storing real-time images of all the culture dishes; bacterial colony identification and counting: loading real-time images of all culture dishes, and automatically identifying bacterial colonies and counting through image preprocessing. The method is used for solving the problem of low accuracy of high-flux detection and improving the flux, counting accuracy and efficiency of simultaneous detection of the culture dish.

The invention relates to a device (30) for counting colonies present in a sample, having a position provided for receiving as a holding sample carrier and a solid medium, comprising: a first detector and a second detector formed by a single detector (35), a processing unit (36) is configured to determine that the bright field arrangement is dominant, the bright diffusion surface (14) replaces the dark diffusion surface (24), a light-emitting diode (13) is extinguished to the dark field, the light-emitting diode (13) is extinguished to the bright field. In the dark field configuration, the dark diffusion surface (24) replaces the bright diffusion surface (14), extinguishing the light-emitting diode (13) in a bright field, and lighting the light-emitting diode (13) in a dark field.

The invention provides a bacterial colony counting method based on machine vision in the field of bacterial colony counting, which comprises the following steps: step S10, acquiring an original photoof a culture dish, and preprocessing the original photo to generate an optimized photo; S20, traversing and searching a first outline of a culture dish in the optimized photo, setting an area outsidethe first outline to be black, and generating a photograph of the culture dish; S30, traversing and searching a second outline of each bacterial colony in the photograph; and step S40, counting the second contours. The bacterial colony counting device has the advantage that the bacterial colony counting efficiency and precision are greatly improved.

The present invention relates to a method for counting lactic acid bacteria, bacillus and yeast in a fermented feed. The method is as below: diluted fermented feed samples are separately counted in anMRS solid medium containing natamycin, a YPD medium containing streptomycin and penicillin, and an LB medium, so that the number of lactic acid bacteria, bacillus and yeast in the fermented feed canbe more accurately counted, and the counting accuracy of single microorganisms in the fermented feed is improved; and the method is easy to operate.

The invention discloses a detection method of staphylococcus aureus in milk through a double signal amplification effect and belongs to the technical field of biosensing. According to the technology,a gold-platinum nano composite material and DNA mimic enzyme are used for synthesizing nano probes which have the good double catalytic effect on H2O2, a modified electrode made of a graphene / gold nanoparticle nano composite material is used so that the electron transfer capacity of the modified electrode can be improved, and accordingly quick high-sensitivity detection for staphylococcus aureus is achieved. Under the optimized experimental conditions, an electrochemical immunosensor has a wide linear detection range for staphylococcus aureus. In addition, the sensor is successfully applied toquantitative detection of staphylococcus aureus in pure milk and yogurt samples. Compared with traditional methods such as a slab colony counting method, the detection method is convenient and fast,easy to operate and high in sensitivity, and quick high-sensitivity detection for staphylococcus aureus is achieved.

The invention relates to an intelligent colony counting incubator and a counting method. The intelligent colony counting incubator comprises a computer assembly, an imaging assembly, a mechanical arm,a culture utensil station, a heating pipe, a recovery assembly, a horizontal bottom plate, a box inlet assembly and a door opening and closing assembly, wherein the computer assembly is in sliding connection with a stand column; the imaging assembly is fixedly connected to the bottom plate in the incubator; the mechanical arm is fixedly connected with the horizontal bottom plate; the culture utensil station is semi-annular, and is fixedly connected with the horizontal bottom plate; the recovery assembly consists of a box outlet part and a recovery part, wherein the box outlet part is fixedlyconnected to the horizontal bottom plate; the recovery part is fixedly connected to the outer side of the box body; the box inlet assembly is fixedly connected with the horizontal bottom plate; and the door opening and closing assembly is fixedly connected to the front inner wall surface of the box body. The intelligent colony counting incubator disclosed by the invention is simple in mechanical structure and reasonable in layout, automation of colony culture, colony imaging counting and colony recovery can be realized, labor is saved, efficiency is improved, cost is reduced, and the intelligent colony counting incubator has important significance in development of colony culture.

The invention provides an induction method of a VBNC state of lactobacillus casei Zhang and a detection method of cell membrane fatty acid in the VBNC state, and belongs to the technical field of microbial culture. The induction method comprises the following steps: inoculating the lactobacillus casei Zhang into a liquid MRS culture medium for activation, and then inoculating the lactobacillus casei Zhang into an MRS induction solution for induction in the VBNC state at 3.5-4.5 DEG C until the number of living cells counted by a plate colony counting method is 0 and the number of living cellscounted by a fluorescence microscope method is greater than 0 so as to obtain the VBNC state of the lactobacillus casei Zhang. By the method, cells in the VBNC state are successfully obtained, and thetypes of the VBNC state induced induction solution of the lactobacillus casei Zhang are enriched. The invention also provides a difference analysis result for extracting the components and contents of the membrane fatty acid in the normal state and the VBNC state of the lactobacillus casei Zhang.

The invention discloses a Bacillus spp culture medium with coconut water as a main raw material. The culture medium comprises: (1) a growth stimulation culture medium ( / L); (2) a liquid amplification culture medium ( / L); and (3) a colony counting culture medium ( / L). According to the invention, economical, cheap and widely available coconut water is used as the main raw material for the Bacillus spp culture medium, and a scientific and reasonable application method of the Bacillus spp culture medium is constructed at the same time; with the application method, the culture medium can meet requirements of scale-up culture of Bacillus spp; a leftover of coconut processing in Hainan province, i.e., coconut water, is made full use of, the Bacillus spp culture medium with coconut water as the main raw material is created for the first time, a good cheap culture medium formula is provided for development of scale-up culture of Bacillus spp in the future, and the Bacillus spp culture medium has a good application prospect; an effective approach is provided for high-valued utilization of coconut water, an added value of coconut processing can be obviously increased, and pollution of the industry of coconut processing to the environment can be reduced at the same time.