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48 results about "Species level" patented technology

Species are as specific as you can get. It is the lowest and most strict level of classification of living things. The main criterion for an organism to be placed in a particular species is the ability to breed with other organisms of that same species. The species of an organism determines the second part of its two-part name.

Method of detecting microorganisms

The present invention relates generally to a method for detecting, enumerating and/or identifying microorganisms in a sample. More particularly, the present invention provides a method for determining total microbial content in a sample by detecting the presence of nucleotide sequences associated with all or part of 16S rDNA or its corresponding 16S rRNA or its homologue, functional equivalent or derivative. The nucleotide sequences of the present invention may be used as an indicator of any microorganism and, hence, represents a universal target sequence which is indicative of total microbial content in a sample. The universal target sequence may also be varied to render same genus or species specific or the universal target used to trap microbial DNA or RNA which may be subsequently analyzed by sequence analysis or genetic probe technology. The universal target sequence is useful inter alia to design as universal primers and probes to amplify any microbial-derived genomic sequence, as a means to detect and enumerate total microorganisms and to identify microorganisms in a sample at the genus or species level. Such uses enable improved methods of enviroprotection, bioremediation, medical diagnosis and industrial microbiology. The present invention further relates to the universal target sequence in isolated form and/or primers or probes capable of hybridizing to same and kits for the detection of total microbial content in a sample.
Owner:THE UNIV OF SYDNEY +1

Method for designing species specific primer for detecting species with known genome information in microbial community and method for measuring bacterium content

The invention relates to a method for designing a species specific primer for detecting the species with known genome information in a microbial community and a method for measuring the bacterium content. The designing method is based on a species specific primer of some species with known genome information in a microbial community. The method comprises the following steps: cloning and sequencing a nearly full-length ribosomal gene library; carrying out biological information analysis (BLAST, etc.) to determine the species in a bacterial community and relative abundance of the species; inquiring the species with known genome sequences; based on a PRIMER-BLAST tool, designing a species specific primer, and detecting the specificity of the primer. The bacterium content is measured through a QPCR method: the provided species specific primer is used to quantitatively measure the content of corresponding species in a microbial community. The provided method for measuring the content of known microbes in a microbial community can trace and inspect the number change of some important microbial species in an important biological process (liquor fermentation, for example) in the species level. The provided method can measure the copy number of non-ribosomal gene sequence of species specificity in some microbial genome and cannot obtain the specific number of cells of species in a microbial community.
Owner:HARBIN INST OF TECH AT WEIHAI

High through-put detection of pathogenic yeasts in the genus trichosporon

The emergence of opportunistic and antifungal resistant strains has given rise to an urgent need for a rapid and accurate method for the detection of fungal pathogens. In this application, we demonstrate the detection of medically important fungal pathogens at the species level. The present method, which is based on a nucleotide hybridization assay, consists of a combination of different sets of fluorescent beads covalently bound to species specific capture probes. Upon hybridization, the beads bearing the target amplicons are classified by their spectral addresses with a 635 nm laser. Quantitation of the hybridized biotinylated amplicon is based on the fluorescent detection with a 532 nm laser. Using this technology we designed and tested various multiplex formats, the performance of forty eight species specific and group specific capture probes designed from sequence analysis in the D1/D2 region of ribosomal DNA, internal transcribed spacer regions (ITS), and intergenic spacer region (IGS). Species-specific biotinylated amplicons (>600 bp) were generated with three sets of primers to yield fragments from the three regions. The developed assay was specific and relatively fast, as it discriminated species differing by one nucleotide and required less than 50 min following amplification to process a 96 well plate with the capability to detect up to 100 species per well. The sensitivity of the assay allowed the detection as low as 102 genome molecules in PCR reactions and 107 to 108 molecules of biotinylated amplification product. This technology provided a rapid means of detection of Trichosporon species and had the flexibility to identify species in a multiplex format by combining different sets of beads. The assay can be expanded to include all known pathogenic fungal species.
Owner:MIAMI UNIVERISTY OF

Method for evaluating dominance of species and application thereof in monitoring of endangered species and diagnosis and risk prediction of human flora-related diseases

The invention relates to the dominance evaluation of ecological communities at the community level and species level, and mainly invents a concept and a method capable of comprehensively evaluating the dominance at the community level and species level. The concept and the method are used to monitor endangered species in macro-ecological communities and to diagnose and predict the risk of human flora-related diseases in microbial communities. The inventor defines a dominance index at the community level, a species dominance distance at the species level and a species dominance index. The threeindexes are defined by a unified mathematical concept and method, and can be used to evaluate the dominance of various biological/ecological communities (including macro-ecological communities (suchas tropical rainforests) and human microbial communities) in various time and/or space scales. In the specification, the microbial flora (community) in the female reproductive tract is taken as an example, and the dominance index is used to study the dominance change of the community in the time scale and the dominance of each species in the community and identify the difference in dominance of the same species in different communities (in the space scale).
Owner:KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI

Fluorescent PCR (polymerase chain reaction) quick detection primer and kit for Xanthomonas campestris pv. campestris

The invention relates to a real-time fluorescent PCR (polymerase chain reaction) quick detection primer and kit for Xanthomonas campestris pv. campestris. The primer comprises a forward primer of which the sequence is SEQ ID NO:1 and a reverse primer of which the sequence is SEQ ID NO:2. In the invention, a specific conserved sequence is obtained through molecular biological analysis according to a pthBXam and Tn3 gene sequence of Xanthomonas campestris pv. manihotis, and a specific amplification primer thereof is designed. The conserved gene sequence is shared by different Xanthomonas campestris pv. manihotis strains to ensure the reliability in detection of Xanthomonas campestris pv. manihotis from different sources on a species level. Besides, the primer provided by the invention adopts fluorescence labeling; and compared with the common PCR technology, observation does not need to be performed through a gel electrophoresis method, thus realizing the integrated sealed detection in the detection process.
Owner:郑媛

Gene chip for performing specific detection on bacillus subtilis

InactiveCN106191078ARealize classification and identificationReliable resultsMicrobiological testing/measurementFermentationFertilizerSpecies level
The invention discloses a gene chip for performing specific detection on bacillus subtilis, and belongs to soil microbe gene detection technology. According to a reported bacillus subtilis 16SDNA sequence and a reported rpoB gene sequence, a bacillus subtilis rpoB-specific gene fragment and a 16S-specific gene fragment are selected as detected target sequences, and corresponding multiple PCR amplification primer pairs, probes and detection chips are designed. Through the gene detection chip, the bacillus subtilis can be qualitatively and quantitatively detected within a short time, and classification and identification of the bacillus subtilis in the species level are achieved. The gene chip provides a powerful tool for colonization and propagation of the bacillus subtilis in fertilizer or soil and the like.
Owner:SHANXI UNIV

Novel detection method of urine bacteria

The invention discloses a novel detection method of urine bacteria. The bacteria in urine are identified by increasing the amount of culture specimens, changing different growth media and gas conditions, and prolonging the culture time of bacteria. The method comprises the following steps: collection of urine; preservation and transportation; preliminary subculture; sub-subculture; and identification of bacteria. According to the present invention, the kind of bacteria in urine can be determined at the species level, and the further biochemical characteristics and metabolic characteristics ofthe cultured bacteria are analyzed and studied; and missing bacteria by standard culture can be identified by the method, anaerobic bacteria, aerobic bacteria and microaerophilic bacteria that survivein urine can be cultured and identified, and the method of the invention can more fully reflect the situation of all bacteria in urine compared with the standard culture. The time required by the method of the present invention is significantly shorter than the time of 16S rDNA sequencing, and the operation is simpler; the requirements for laboratory and experimental equipment are relatively low,which can be realized by most hospitals and laboratories; and the method has high operability and popularization.
Owner:NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV

Application of madder polysaccharide in preparation of health-protection food with effects of preventing and treating neurodegenerative diseases

The invention discloses application of a madder polysaccharide in the preparation of a health-protection food with effects of preventing and treating neurodegenerative diseases. The madder polysaccharide can be a commercially available product or a product prepared by an applicant and is preferably a madder acidic polysaccharide. The invention also discloses a preparation method of the madder acidic polysaccharide. The madder acidic polysaccharide prepared by using the method disclosed by the invention has the characteristics of high polysaccharides content, low impurity content and high uniformity. Furthermore, the preparation method is simple and high in efficiency and is suitable for industrial production. Experiments on an in-vitro aggregation model, mammalian cells and a caenorhabditis elegans disease model show that the madder polysaccharide can effectively suppress the abnormal aggregation of polyglutamine and beta-amyloid proteins and the neurotoxicity of the polyglutamine and the beta-amyloid proteins and also obviously reduce the reactive oxide species level of the disease model, so that the madder polysaccharide has a good preventing and treating effect on the neurodegenerative diseases.
Owner:INFINITUS (CHINA) CO LTD

Interpretation method based on pathogen metagene next-generation sequencing

The invention discloses an interpretation method based on pathogen metagene next-generation sequencing. A large number of detected sequences accord with at least one of the following sequences: coverage: the seed level being greater than 10 times of that of any other seed; the abundance being greater than 60%; sequence number: under the condition that the value of the total sequence number is normal, SMRN or SDMRN being greater than 10,000. A medium number of detected sequences accord with at least one of the following sequences: coverage: the species level being 4-10 times of that of any other species; abundance: 40%-60%; sequence number: under the condition that the value of the total sequence number is normal, the SMRN or SDMRN: 5,000-10,000. A small number or an extremely small numberof detected sequences accord with at least one of the following sequences: colonization and pathopoiesis cannot be distinguished; the bacteria are not common background bacteria of the specimens or donot accord with general characteristics of the background bacteria. The conclusion is more objective, the interpretation can be conducted at any time according to the standard, the method has the clinical practicability and objectivity, and the reported conclusion can be directly used for clinical practice.
Owner:ZHONGSHAN HOSPITAL FUDAN UNIV

A kind of m65 resistance welding sleeve and its manufacturing method

The invention discloses an M65-grade electric resistance welding casing pipe and a manufacturing method thereof. The M65-grade electric resistance welding casing pipe comprises, by weight percentage, 0.14-0.18% of C, 0.15-0.30% of Si, 1.10-1.40% of Mn, less than or equal to 0.020% of P, less than or equal to 0.008% of S, 0.06-0.12% of Ti, 0.02-0.05% of Als, less than or equal to 0.008% of N, less than or equal to 0.40% of carbon equivalents and the balance Fe and unavoidable elements. A continuous casting sheet billet is heated to 1150-1200 DEG C through a heating furnace and subjected to hot continuous rolling. The finishing rolling temperature is 780-840 DEG C. After being rolled, a steel belt is cooled at the speed of 8-12 DEG C / s. The steel belt is coiled at the temperature of 560-620 DEG C. The steel belt is subjected to high frequency / medium frequency electric resistance welding and made into a steel pipe through an ERW unit. The whole pipe is subjected to the normalizing heat treatment process, wherein the whole pipe is heated to 880-910 DEG C, the temperature is preserved for 30-50 minutes, and air cooling is conducted. The steel belt is good in weldability and impact toughness; normalizing treatment is adopted, so that banded structures and residual stress are eliminated; the heat treatment process is simplified; and performance consistency of a pipe body and weld joints is guaranteed.
Owner:ANGANG STEEL CO LTD
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