Interpretation method based on pathogen metagene next-generation sequencing

Abstract

The invention discloses an interpretation method based on pathogen metagene next-generation sequencing. A large number of detected sequences accord with at least one of the following sequences: coverage: the seed level being greater than 10 times of that of any other seed; the abundance being greater than 60%; sequence number: under the condition that the value of the total sequence number is normal, SMRN or SDMRN being greater than 10,000. A medium number of detected sequences accord with at least one of the following sequences: coverage: the species level being 4-10 times of that of any other species; abundance: 40%-60%; sequence number: under the condition that the value of the total sequence number is normal, the SMRN or SDMRN: 5,000-10,000. A small number or an extremely small numberof detected sequences accord with at least one of the following sequences: colonization and pathopoiesis cannot be distinguished; the bacteria are not common background bacteria of the specimens or donot accord with general characteristics of the background bacteria. The conclusion is more objective, the interpretation can be conducted at any time according to the standard, the method has the clinical practicability and objectivity, and the reported conclusion can be directly used for clinical practice.

Description

technical field [0001] The invention belongs to the technical field of gene sequencing, and in particular relates to an interpretation method based on next-generation sequencing of pathogenic macrogenes. Background technique [0002] In the past two years, the new pathogen macrogene next-generation sequencing detection method at home and abroad, the quality of the basic experimental process is still controllable, but there is no unified standard for the interpretation of the final bioinformatics data, and the technical staff of the detection company cannot combine the clinical front-line patient data. The subjective factors of data interpretation are relatively large, and there is no uniform standard in the industry, which makes the report accuracy of this technology low and the clinical practicability is poor, which seriously affects the improvement and promotion of this technology. Contents of the invention [0003] In view of this, the purpose of the present invention i...

AI Technical Summary

Problems solved by technology

[0002] In the past two years, the new pathogen macrogene next-generation sequencing detection method at home and abroad, the quality of the basic experimental process is still controllable, but there is no unified standard for the interpretation of the final bioinformatics data, and the technical staff of the detection company cannot combine the clinical front-line patient data. The subjective factors of data interpretation are relatively large, and there is no uniform standard in the industry, which makes the report accuracy of this technology low and the clinical practicability is poor, which seriously affects the improvement and promotion of this technology