Novel detection method of urine bacteria

A technology of bacteria detection and urine, applied in the field of medical detection, can solve the problems of low sensitivity, drug-resistant bacteria, precise guidance of clinical treatment, etc., and achieve the effect of simple operation, shortened time, high operability and popularization

Pending Publication Date: 2019-12-31
NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
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Problems solved by technology

On the one hand, the low sensitivity of standard urine culture brings unnecessary burden to patients; on the other hand, because most of the urine culture results of patients are negative, it cannot provide accurate guidance for clinical treatment, forcing doctors to use antibiotics empirically, which may cause Emergence of resistant bacteria

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  • Novel detection method of urine bacteria
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Embodiment 1

[0044] Embodiment 1 Novel urine culture technique of the present invention

[0045] 1) Urine collection: Disinfect the perineum with 0.5% povidone iodine solution and other disinfectants, insert the urinary catheter into the bladder through the urethra, first discard about 15ml of the front urine, and then keep the middle urine in 50ml sterile , clean, leak-free, particle-free EP tube;

[0046] 2) Preservation and transportation: Urine samples should be analyzed within 2 hours after collection;

[0047] 3) Preliminary subculture: Inoculate and subculture in a biological safety cabinet to strictly avoid specimen contamination. The method is as follows:

[0048] (1) Take 100ul urine samples and spread them on blood agar plates, chocolate plates, and Columbia CNA blood agar plates, and then place the culture plates in 5% CO at 35°C 2 Cultivate in a gas environment for 48 hours;

[0049] (2) Take 100ul of urine specimen and smear it on a blood agar plate, and then place the cul...

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Abstract

The invention discloses a novel detection method of urine bacteria. The bacteria in urine are identified by increasing the amount of culture specimens, changing different growth media and gas conditions, and prolonging the culture time of bacteria. The method comprises the following steps: collection of urine; preservation and transportation; preliminary subculture; sub-subculture; and identification of bacteria. According to the present invention, the kind of bacteria in urine can be determined at the species level, and the further biochemical characteristics and metabolic characteristics ofthe cultured bacteria are analyzed and studied; and missing bacteria by standard culture can be identified by the method, anaerobic bacteria, aerobic bacteria and microaerophilic bacteria that survivein urine can be cultured and identified, and the method of the invention can more fully reflect the situation of all bacteria in urine compared with the standard culture. The time required by the method of the present invention is significantly shorter than the time of 16S rDNA sequencing, and the operation is simpler; the requirements for laboratory and experimental equipment are relatively low,which can be realized by most hospitals and laboratories; and the method has high operability and popularization.

Description

technical field [0001] The invention belongs to the technical field of medical detection and relates to a novel method for detecting bacteria in urine. Background technique [0002] The human body is not a single individual. There are a large number of microorganisms with complex structures, including bacteria, archaea, protists, fungi, and viruses. They colonize the gastrointestinal tract, oral cavity, skin, urogenital tract, and respiratory tract. The cumulative dry weight of human microorganisms is only about 1% to 2% of the total body weight, but it has a huge total amount of genes, and the number of encoded genes can reach 150 times the number of human genes, which is equivalent to the "second genome" of the human body. Microorganisms are an important part of the microenvironment of the human body, and play a key role in the maintenance of the body's health and the occurrence of diseases. Behind the occurrence of human diseases, there may be factors of microecological i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04
CPCC12Q1/04
Inventor 吴芃曾嘉荣赵洁陈春晓李坤温粤辉
Owner NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
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