Preparation method of liver organoid
An organoid and liver technology, applied in biochemical equipment and methods, bone/connective tissue cells, artificial cell constructs, etc., and can solve problems such as micro-efficiency
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Embodiment 1
[0048] Method for reprogramming umbilical cord mesenchymal stem cells into liver cells
[0049] Umbilical cord is derived from human beings, but it is usually treated as medical waste. Its sources are extensive and do not involve ethical issues. It is a superior tissue source for obtaining a large number of mesenchymal stem cells in clinical and research. Umbilical cord mesenchymal stem cells (UC-MSCs) have strong proliferative activity and have been used in the treatment of various diseases.
[0050] In this application, UC-MSCs were cultured to 6 points under serum-free conditions, that is, UC-MSCs were cultured in vitro until entering the logarithmic growth phase, such as figure 1 As shown, when UC-MSCs were cultured in vitro to the third passage, their morphology was uniform and they grew in the shape of a long spindle-shaped womb. Subsequent identification of cell surface markers and differentiation ability of UC-MSCs confirmed that the extracted UC-MSCs were in line with...
Embodiment 2
[0062] Liver organoid and preparation method thereof
[0063] Organoids are three-dimensional cell cultures that contain some of the key properties of their representative organs. Organoids are also an in vitro culture system that includes a self-renewing stem cell population that can differentiate into multiple organ-specific cell types, has a similar spatial organization to the corresponding organ and can reproduce some of the functions of the corresponding organ, thereby providing a Highly physiologically relevant system. Organoid cultures have been used in a variety of tissues, including gut, liver, pancreas, kidney, prostate, lung, optic cup, and brain. Organoids are also a tool with applications in developmental biology, disease pathology, cell biology, mechanisms of regeneration, precision medicine, and drug toxicity and efficacy testing, among others. For these applications and others, organoid culture provides a highly informative complement to existing two-dimensio...
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