10 results about "Serum free medium" patented technology

This invention proposes a method for preparing dental pulp stem cell exosomes and their applications, belonging to the field of exosome technology. Dental pulp stem cells from exfoliated deciduous teeth are seeded into serum-free α-MEM medium containing microparticles, insulin-like growth factor, transforming growth factor-β1, penicillin, and streptomycin for culture. Exosomes are isolated, dispersed in PBS solution, and incubated with microparticles, propylene glycol, and rhodioloside to obtain highly active dental pulp stem cell exosomes. The surface of these exosomes is then modified with shikonin and interferon to obtain dental pulp stem cell exosomes. The dental pulp stem cell exosomes prepared by this invention exhibit good affinity for titanium implant-mediated osteointegration repair materials, demonstrating good anti-inflammatory, immunomodulatory, osteogenic, and damage-reducing effects. Furthermore, the preparation method is simple, yields high output, and operates under mild conditions, showing broad application prospects.

The method of hair transplant using dermal papilla and dermal sheath obtained from hair follicle as disclosed comprises the steps of: providing initial keratinocytes to be used for creating condition medium; creating and collecting condition medium from said keratinocytes; collecting hair follicle cells containing dermal papilla and dermal sheath from patients; culturing said cells as obtained from patients using condition medium collected from the prior step; injecting cultured cells in the predetermined amount and the predetermined ratio of dermal papilla cells and dermal sheath cells in the predetermined amount area characterized in that the step of injecting and collecting condition medium from said keratinocytes comprises the step of applying a solution obtained from the mixture of defined keratinocyte-serum free medium (DK-SFM) and Mesenchymal stem cell culture medium (MSC) to said initial keratinocytes, removing defined keratinocyte-serum free medium (DK-SFM), and extracting a condition medium from the said mixture and wherein the step of culturing hair follicle cells as obtained from patients comprises the step of applying condition medium extracted and MSC in the ratio of 1:1.

The application belongs to the technical field of biological medicine, and particularly relates to umbilical cord mesenchymal stem cells and a preparation method and application thereof. The preparation method takes umbilical cord Wharton's jelly as raw material, and obtains high-purity umbilical cord mesenchymal stem cells through cleaning treatment, tissue block preparation, serum-free culture and liquid change subculture. The culture conditions and operation parameters are strictly controlled in the preparation process, the MSC serum-free culture medium and trypsin substitutes are used, so that the cell activity and purity can be guaranteed. The umbilical cord mesenchymal stem cells have no obvious toxicity and tumorigenic risk under a safe dose, and have no damage to organs in long-term use. The umbilical cord mesenchymal stem cells can significantly improve the motor dysfunction after cerebral ischemia, reduce the volume of cerebral ischemic injury, protect the integrity of the blood-brain barrier, reduce the neuroinflammatory response, and play a multi-dimensional role in brain tissue protection and repair, thereby providing a new effective means for the treatment of ischemic stroke, and having important clinical transformation value and application prospect.

Provided are a serum-free medium for co-incubation of tumor organoids and immune cells, a tumor organoid immune microenvironment system and a construction method therefor, and a method for evaluating the efficacy of drugs based on tumor organoid immune co-culturing. The used medium for co-incubation of tumor organoids and immune cells can well maintain the growth of tumor organoids and immune cells, and can provide nutritional support for these two different types of cells. In addition, the established method for evaluating the efficacy of drugs in a tumor organoid immune co-culture system can be used for evaluating the efficacy and toxic side effects of an immune drug against a tumor, helping to select potentially effective drugs and optimize a treatment scheme. By means of the organoid immune co-culture system, the response of different patients to drugs can be better understood, providing a basis for personalized medicine and precision treatment.

The invention relates to neurobiology, molecular virology, biomedical engineering and the like, and discloses a domesticated low-serum Vero cell line and a method for preparing an HSV virus vector through suspension culture cell amplification. Aiming at the bottleneck problem that a process for amplifying HSV by adherent culture of Vero cells is difficult to realize large-scale and engineering amplified production, the invention researches and develops a method for preparing HSV based on suspension culture of cell amplification. Vero cells introduced from ATCC are domesticated, and the serum content of a culture medium is gradually reduced, so that the Vero cells are suitable for suspension culture in a microcarrier or sheet carrier reactor by using a low-serum or serum-free culture medium. The method comprises the following steps: using Tamp of Dibide Bioengineering (Shanghai) Co., Ltd; and carrying out suspension culture of the Vero cells and infection amplification of the HSV virus vector by using a J Cloud Ready TM bioreactor. High-activity suspension culture and HSV amplification of Vero cells are realized by optimizing production conditions, cell density, biochemical detection and the like, and a stable and efficient method is provided for preparing a high-titer and high-purity HSV virus vector in combination with purification process upgrading.

[Problem] To provide a method for producing a culture supernatant liquid, the method making it possible to produce a large amount of a culture supernatant liquid containing an active ingredient at a high concentration. [Solution] The method for producing a culture supernatant liquid comprises: (a) a step for supplying a culture solution containing a carrier, mesenchymal stem cells, and serum (hereinafter referred to as "FBS-DMEM") to a culture tank; (b) a step for adhering the mesenchymal stem cells to the carrier; (c) a step for culturing the mesenchymal stem cells using the FBS-DMEM; (d) a step for removing the FBS-DMEM while leaving the carrier-adhered mesenchymal stem cells in a filter; (e) a step for washing the culture tank, the carrier-adhered mesenchymal stem cells, and the filter; (f) a step for supplying a serum-free culture solution for a supernatant liquid (hereinafter, referred to as "CM-DMEM") to the culture tank; (g) a step for culturing the mesenchymal stem cells using the CM-DMEM; and (h) a step for recovering the CM-DMEM while leaving the carrier-adhered mesenchymal stem cells in the filter.

The invention relates to the technical field of biological medicine, in particular to an autophagosome for promoting hair growth and an extraction method and application thereof. Comprising the following steps: inoculating human umbilical vein endothelial cells or human placenta mesenchymal cells into a DMEM (Dulbecco Modified Eagle Medium) complete medium, and culturing until the cell fusion degree reaches 80-90%; replacing the DMEM complete culture medium with a serum-free DMEM culture medium, collecting supernate after culture is completed, and centrifuging the supernate to obtain a crude extract containing the autophagosome; and resuspending the crude extract by using PBS, adding an LC3 antibody, and finely extracting by using a paramagnetic particle method to obtain the autophagosome. The method has the advantages of simple operation process, no need of other inducers in the process of inducing the formation of the autophagosome, no too high requirements on laboratories, and high yield of the autophagosome, and provides an efficient, convenient, economical and practical treatment means for alopecia patients.

The invention belongs to the technical field of poultry vaccines, and particularly relates to a chicken new tributary method adeno-quintuplet vaccine and a preparation method thereof.The chicken new tributary method adeno-quintuplet vaccine comprises chicken Newcastle disease venom, infectious bronchitis venom, avian influenza venom, infectious bursal disease virus liquid and fowl adenovirus venom, and the preparation method of the Newcastle disease venom comprises the steps that firstly, frozen MDCK suspension cells are provided; the frozen MDCK suspension cells are recovered at the temperature of 40-45 DEG C; 2, transferring the resuscitated MDCK suspension cells to a serum-free culture medium; according to the invention, the inactivated vaccine is prepared from the Newcastle disease venom, the infectious bronchitis venom, the avian influenza venom, the infectious bursal disease virus liquid and the fowl adenovirus venom by adopting a superstrong concentration process and a high-quality adjuvant, so that after animals are immunized, antibodies are generated quickly, the titer is high, the protection period is long, and the occurrence and spreading of epidemic diseases are prevented; and people can conveniently put multi-combined vaccines to cope with continuously changing epidemic disease prevention and control situations.

The invention discloses a serum-free culture medium and application thereof in culture of large yellow croaker muscle stem cells. The serum-free culture medium comprises a basic culture medium and an exogenous additive component, wherein the exogenous additive components comprise HEPES, sodium chloride, hydroxypropyl-beta-cyclodextrin, sodium metavanadate, vitamin C magnesium phosphate, omega-3 polyunsaturated fatty acid, pyridoxine hydrochloride, nicotinamide, cholesterol, calcium chloride, potassium chloride, a soybean pancreatin inhibitor, taurine, trehalose, ethanolamine, a telomerase activator and recombinant human serum albumin. The animal-derived protein-free serum-free culture medium disclosed by the invention does not contain animal-derived components such as serum, does not need to add components such as growth factors or hormones, not only has definite chemical components, high safety, low cost and high cell proliferation speed, but also can effectively improve the myogenic differentiation potential of muscle stem cells and effectively activate telomerase activity to solve the problem of cell aging; and a feasible product and a feasible method are provided for culture of fish cells and large-scale production of cell culture meat.

This invention discloses a culture medium and method suitable for large-scale full-suspension culture of Vero cells. The culture medium is a serum-free medium specifically designed and developed based on the growth and metabolic characteristics of Vero suspension cells and their different proliferation abilities when cultured in a reactor versus a shaker, combined with the differences in culture scale. It includes the following components: amino acids, proteins, vitamins, inorganic salts, serum replacement factors, and auxiliary components. Furthermore, this invention determines the control parameters of the full-suspension culture reactor based on cell growth characteristics, significantly increasing the VERO cell culture density. The cell doubling rate reaches 10-fold after 48 hours of culture, the cell production cycle is halved, and production efficiency is improved. The operation is simple, reducing the contamination rate in large-scale culture and solving the problem of long perfusion culture cycles. This is of great significance for the large-scale production of viral vaccines using VERO cells as a substrate.