Serum-free medium used for culture of elderly people cartilage cells

A serum-free medium, chondrocyte technology, applied in the direction of bone/connective tissue cells, animal cells, vertebrate cells, etc., can solve the non-physiological response, proliferation and differentiation ability of cultured cells far less than young people, cell therapy methods It can not effectively meet the problems such as maintaining joint function and facilitating large-scale promotion and application.

Inactive Publication Date: 2014-10-29
HANGZHOU LONGHILL BIO MEDICATION TECH
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  • Summary
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Problems solved by technology

In fact, the cartilage tissue taken from the non-weight-bearing area of ​​elderly patients, the chondrocytes isolated and expanded in vitro, have far less proliferation and differentiation ability than young people. At present, there are a large number of elderly patients who need treatment for articular cartilage, but now Some feasible cell therapy methods cannot effectively meet this demand
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Method used

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  • Serum-free medium used for culture of elderly people cartilage cells
  • Serum-free medium used for culture of elderly people cartilage cells
  • Serum-free medium used for culture of elderly people cartilage cells

Examples

Experimental program
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Embodiment 1

[0065] Embodiment 1. A kind of serum-free medium for cultivating chondrocytes of aged people comprises basal medium and additive, and basal medium is DMEM / F12 (1:1), and the composition of this additive includes in final concentration:

[0066] 250 μM Vitamin C,

[0067] 4.5 μM linoleic acid,

[0068] 13 µM cholesterol,

[0069] 10nM dexamethasone,

[0070] 50 μM acetylcysteine,

[0071] 25 μg / mL transferrin,

[0072] 30nM sodium selenite,

[0073] 17 μM sodium pantothenate,

[0074] 33 μM biotin,

[0075] 10 μg / mL of insulin,

[0076] 5ng / mL of epidermal growth factor.

[0077] 5ng / mL of fibroblast growth factor,

[0078] 5 ng / mL platelet-derived growth factor,

[0079] 1% human serum albumin.

Embodiment 2

[0080] Embodiment 2. A kind of serum-free medium for cultivating chondrocytes of aged people comprises basal medium and additive, and basal medium is DMEM / F12 (1:1), and the composition of this additive includes in final concentration:

[0081] 240 μM Vitamin C,

[0082] 4 μM linoleic acid,

[0083] 15 µM cholesterol,

[0084] 10nM dexamethasone,

[0085] 45 μM acetylcysteine,

[0086] 20 μg / mL transferrin,

[0087] 25nM sodium selenite,

[0088] 18 μM sodium pantothenate,

[0089] 35 μM biotin,

[0090] 10 μg / mL of insulin,

[0091] 5ng / mL of epidermal growth factor,

[0092] 5ng / mL fibroblast growth factor,

[0093] 5ng / mL platelet-derived growth factor,

[0094] 1% human serum albumin.

[0095] In the above examples, DMEM / F12 (1:1) is a culture medium produced by GIBCO Company, product number: 11330057.

[0096] Among the additive ingredients mentioned above,

[0097] 233μM ~287μM vitamin C is used as a biologically active substance to maintain cell growth, reg...

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Abstract

The invention discloses a serum-free medium used for culture of elderly people cartilage cells, the-free medium comprises a basal medium and an additive comprising the components of the following concentration: 233muM-287 muM of vitamin C, 3.7mu M-8.9 muM of linoleic acid, 9muM-17 muM of cholesterol, 6nM-15 nM of dexamethasone, 43 muM-58mu M of acetyl cysteine, 18 mug / mL-32 mu g / mL of transferrin, 22nM-52 nM of sodium selenite, 13 muM-24 muM of sodium pantothenate, 28 muM-43 muM of biotin, 7 mug / mL-18 mu g / mL of insulin, 2 ng / mL-9 ng / mL of epidermal growth factor, 2 ng / mL-9 ng / mL of fiber growth factor, 2 ng / mL-9 ng / mL of platelet derived growth factor, and 0.5%-2.5% of human serum albumin. The cell culture time is short, and the cell biological performance is good.

Description

technical field [0001] The present invention relates to a serum-free medium for culturing human articular chondrocytes in vitro, in particular to a serum-free medium for cultivating chondrocytes of aged people. Background technique [0002] Articular cartilage damage, whether caused by trauma or pathology, cannot be repaired spontaneously, and usually leads to degenerative changes of cartilage, which in turn leads to osteoarthritis. For the elderly, the only way to effectively relieve pain and restore knee function is artificial knee replacement. Although this method can relieve pain symptoms to a certain extent, it is similar to "invasive" surgical methods and the limited lifespan of artificial joints. , will make the patient's joint function not be maintained for a long time. [0003] At present, the application of autologous chondrocyte transplantation (ACI) technology can treat such traumatic (or osteochondritis dissecans) knee articular cartilage defects. In recent yea...

Claims

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Application Information

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IPC IPC(8): C12N5/077
Inventor 孙冬梅杨丹何宏森柴勋
Owner HANGZHOU LONGHILL BIO MEDICATION TECH
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