Serum-free medium used for culture of elderly people cartilage cells

Abstract

The invention discloses a serum-free medium used for culture of elderly people cartilage cells, the-free medium comprises a basal medium and an additive comprising the components of the following concentration: 233muM-287 muM of vitamin C, 3.7mu M-8.9 muM of linoleic acid, 9muM-17 muM of cholesterol, 6nM-15 nM of dexamethasone, 43 muM-58mu M of acetyl cysteine, 18 mug / mL-32 mu g / mL of transferrin, 22nM-52 nM of sodium selenite, 13 muM-24 muM of sodium pantothenate, 28 muM-43 muM of biotin, 7 mug / mL-18 mu g / mL of insulin, 2 ng / mL-9 ng / mL of epidermal growth factor, 2 ng / mL-9 ng / mL of fiber growth factor, 2 ng / mL-9 ng / mL of platelet derived growth factor, and 0.5%-2.5% of human serum albumin. The cell culture time is short, and the cell biological performance is good.

Description

technical field [0001] The present invention relates to a serum-free medium for culturing human articular chondrocytes in vitro, in particular to a serum-free medium for cultivating chondrocytes of aged people. Background technique [0002] Articular cartilage damage, whether caused by trauma or pathology, cannot be repaired spontaneously, and usually leads to degenerative changes of cartilage, which in turn leads to osteoarthritis. For the elderly, the only way to effectively relieve pain and restore knee function is artificial knee replacement. Although this method can relieve pain symptoms to a certain extent, it is similar to "invasive" surgical methods and the limited lifespan of artificial joints. , will make the patient's joint function not be maintained for a long time. [0003] At present, the application of autologous chondrocyte transplantation (ACI) technology can treat such traumatic (or osteochondritis dissecans) knee articular cartilage defects. In recent yea...

AI Technical Summary

Problems solved by technology

In fact, the cartilage tissue taken from the non-weight-bearing area of ​​elderly patients, the chondrocytes isolated and expanded in vitro, have far less proliferation and differentiation ability than young people. At present, there are a large number of elderly patients who need treatment for articular cartilage, but now Some feasible cell therapy methods cannot effectively meet this demand

[0004] In addition, the traditional in vitro culture conditions of chondrocytes - using autologous serum or bovine serum (for research) as the medium supplement component, because the cartilage tissue in the human body survives without blood supply and only relies on cell autocrine or Paracrine factors to maintain metabolic homeostasis, which often elicit nonphysiological responses in cultured cells

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