2 results about "Autologous serum" patented technology

The application relates to a serum-loaded ionic catgut and a preparation method thereof, and belongs to the technical field of traditional Chinese medicine external treatment. The preparation method of the serum-loaded ionic catgut comprises the following steps: performing ionic surface treatment on medical absorbable catgut to obtain ionic catgut, and immersing the ionic catgut in autologous serum of a patient to obtain the serum-loaded ionic catgut after soaking. The serum-loaded ionic catgut takes the autologous serum of the patient as a core treatment material, has excellent biocompatibility and no rejection risk, and has completely individualized active ingredients, so that the serum-loaded ionic catgut can adjust to the pathological state of the patient itself, breaks through the limitation of traditional buried thread "one drug thread is universal", and realizes truly individualized precision treatment.

The application discloses a TIL cell high-efficiency amplification method and application, and belongs to the technical field of cell biology. In the initial culture stage, a specific concentration of IL-2, IL-15 and anti-CD3 monoclonal antibody combination is adopted, and autologous serum is added, so that the TIL cells can be effectively activated and initial proliferation of the TIL cells is promoted. In the large-scale amplification stage, IL-7, IL-21 and mesenchymal stem cell conditioned medium are introduced; the mesenchymal stem cell conditioned medium contains various nutritional components and growth factors, and the mesenchymal stem cell conditioned medium and the cytokines synergistically act, so that the amplification efficiency of the TIL cells is significantly improved; compared with a traditional method, the amplification multiple can be increased by 3-5 times, and the culture cycle is shortened by 5-7 days. Through optimization of a cell separation step, improvement of a culture system and regulation of a culture condition, the amplification efficiency, activity and killing capacity of the TIL cells are significantly improved, and the culture cycle is shortened.