8 results about "Autologous serum" patented technology

The invention discloses a gamma delta T cell efficient amplification method and application, and belongs to the technical field of cell biology. The amplification method comprises the following steps: separating PBMC (peripheral blood mononuclear cells) by adopting a density gradient centrifugation method, carrying out initial culture through a polylysine coated container, carrying out three-stage dynamic stimulation, combining with an X-VIVO 15 culture medium of 5-8% autoserum, and finally carrying out anti-gamma delta TCR magnetic bead separation to obtain high-purity cells. Through collaborative optimization of stepped factor combination, staged container coating and a low-serum system, the amplification multiple of the gamma delta T cells reaches 150-180 times, the purity is larger than or equal to 90% after purification, the cytotoxicity and the survival ability are remarkably improved, the gamma delta T cells can be efficiently used for immunotherapy of tumors and infectious diseases, and stable technical support is provided for clinical transformation of the gamma delta T cells.

The invention discloses a ferroptosis inhibition research method based on a hypertonic dry eye cell model, and relates to the technical field of xerophthalmia. Comprising the following steps: S1, establishing a hypertonic dry eye cell model, and selecting human corneal epithelial cells to perform hypertonic treatment in a hypertonic culture medium; s2, detecting a ferroptosis marker; performing GPX4 protein expression detection; s2.1, detecting the lipid peroxidation level; s2.2, detecting the iron ion level of the sample; s2.3, observing the mitochondrial morphology of the sample; s3, carrying out an autoserum intervention experiment, and treating the culture media of different groups by using autoserum; s4, data statistics and analysis; by detecting expression of proteins such as a ferroptosis marker GPX4 and the like, it is determined that autoserum can inhibit ferroptosis and improve corneal epithelial cell survival by enhancing GPX4 expression and reducing lipid peroxidation and iron ion level, a scientific basis is provided for further optimization and accurate application, a treatment strategy for xerophthalmia is improved, and the application has a wide application prospect. And a new direction is opened up for mechanism research and personalized treatment of the xerophthalmia in the future.

An autoserum eye drop administration device comprises a bottle body, a bottom cover and a bottle mouth, an arc-shaped tunnel is arranged in the bottle body, the upper end of the arc-shaped tunnel extends to the bottle mouth, the lower end of the arc-shaped tunnel extends to the bottom cover, a liquid medicine capsule is contained in the arc-shaped tunnel, a separation opening is formed in the side wall of the arc-shaped tunnel, one end of an elastic button is exposed out of the bottle body, and the other end of the elastic button extends to the separation opening. Extruding the liquid medicine capsule; the bottle neck is in threaded connection with the bottle body, a fine needle is arranged in the bottle neck, a fine needle reserved opening is formed in the upper portion of the arc-shaped tunnel, and the bottle neck is rotated to enable the fine needle to enter the arc-shaped tunnel from the fine needle reserved opening and pierce the liquid medicine capsule. By means of the structure, the autoserum eye drop dosing device can be matched with a liquid medicine capsule to conduct autoserum eye drop dosing, pollution is not prone to occurring, the dosing frequency can be increased, and waste is not prone to being caused.

The application relates to a serum-loaded ionic catgut and a preparation method thereof, and belongs to the technical field of traditional Chinese medicine external treatment. The preparation method of the serum-loaded ionic catgut comprises the following steps: performing ionic surface treatment on medical absorbable catgut to obtain ionic catgut, and immersing the ionic catgut in autologous serum of a patient to obtain the serum-loaded ionic catgut after soaking. The serum-loaded ionic catgut takes the autologous serum of the patient as a core treatment material, has excellent biocompatibility and no rejection risk, and has completely individualized active ingredients, so that the serum-loaded ionic catgut can adjust to the pathological state of the patient itself, breaks through the limitation of traditional buried thread "one drug thread is universal", and realizes truly individualized precision treatment.

The invention provides an endometrial stem cell preparation as well as a preparation method and application thereof, and belongs to the technical field of biological preparations. According to the preparation method of the endometrial stem cell preparation, the safety risk is controlled through an autoserum source, the cell function is optimized in combination with a low-oxygen environment, and a high-stability and low-immune-risk standardized solution is provided for clinical transformation of the endometrial stem cell preparation. Three batches of culture data show that the cell viability is gt; the mesenchymal stem cell culture medium is stable in form and meets the mesenchymal stem cell verification standard. The flow detection verification shows that the positive rate of CD73 / CD90 / CD105 is greater than or equal to 95%, the negative markers such as CD34 / CD45 and the like are less than or equal to 2%, and the high purity is proved; the cell cycle shows that the G0 / G1 period accounts for 74-80%, and the resting state and the genome stability are maintained. After the preparation provided by the invention is stored for a short time or frozen and unfrozen for a long time, the cell morphology and proliferation capacity are not obviously attenuated.

The application discloses a TIL cell high-efficiency amplification method and application, and belongs to the technical field of cell biology. In the initial culture stage, a specific concentration of IL-2, IL-15 and anti-CD3 monoclonal antibody combination is adopted, and autologous serum is added, so that the TIL cells can be effectively activated and initial proliferation of the TIL cells is promoted. In the large-scale amplification stage, IL-7, IL-21 and mesenchymal stem cell conditioned medium are introduced; the mesenchymal stem cell conditioned medium contains various nutritional components and growth factors, and the mesenchymal stem cell conditioned medium and the cytokines synergistically act, so that the amplification efficiency of the TIL cells is significantly improved; compared with a traditional method, the amplification multiple can be increased by 3-5 times, and the culture cycle is shortened by 5-7 days. Through optimization of a cell separation step, improvement of a culture system and regulation of a culture condition, the amplification efficiency, activity and killing capacity of the TIL cells are significantly improved, and the culture cycle is shortened.

The invention discloses a TIL cell efficient amplification method and application, and belongs to the technical field of cell biology. In the initial culture stage, a combination of IL-2, IL-15 and anti-CD3 monoclonal antibodies with specific concentrations is adopted, and autoserum is added, so that TIL cells can be effectively activated, and initial proliferation of the TIL cells is promoted. In the large-scale amplification stage, IL-7, IL-21 and a mesenchymal stem cell conditioned culture medium are introduced, the mesenchymal stem cell conditioned culture medium contains various nutritional ingredients and growth factors and has a synergistic effect with cell factors, the amplification efficiency of the TIL cells is remarkably improved, and compared with a traditional method, the amplification multiple can be improved by 3-5 times, and the culture period is shortened by 5-7 days. By optimizing the cell separation steps, improving the culture system and regulating and controlling the culture conditions, the amplification efficiency, activity and killing ability of the TIL cells are remarkably improved, and the culture period is shortened.

To provide a method for producing a mature adipocyte-containing composition which has high revascularization ability and low possibility of inducing inflammation.SOLUTION: A method for producing mature adipocyte-containing composition includes: a cutting and filtering step of cutting and filtering an adipose tissue to which enzyme treatment is not performed to obtain micro-fat; a micro-fat culturing step of culturing micro-fat by using a culture medium after the cutting and filtering step to obtain a mature adipocyte; and a mature adipocyte-containing composition acquisition step of obtaining a mature adipocyte-containing composition containing the mature adipocyte and a conditioned medium obtained by subjecting micro-fat to the micro-fat culturing step, where the culture medium contains self serum or contains FBS, hydrocortisone and FGF-2.SELECTED DRAWING: Figure 1