99 results about "Cartilage cells" patented technology

The invention relates to a medical simulation experiment method and device for acting apoptosis vesicles on primary cartilage cells based on cell line culture, and the method mainly comprises the following steps: S1, co-culture system construction, S2, multi-dimensional observation and analysis, and S3, operation risk assessment. In the step S1, primary cartilage cells, primary immune cells and cell line culture apoptosis vesicles are co-cultured; s2, carrying out multi-dimensional observation and analysis on the influence of apoptosis vesicles on cells; and S3, mainly constructing a multi-dimensional risk scoring system, and carrying out risk grade division by applying a biological network model. The invention aims at simulating and evaluating the influence of cell line culture apoptosis vesicles on primary cartilage cells in vitro and the interaction between the cell line culture apoptosis vesicles and primary immune cells, so that an experimental basis is provided for subsequent clinical application.

The invention belongs to the field of nano-enzyme preparation and biological application, and particularly relates to a preparation method and application of M2 type macrophage membrane coated FeMn diatomic nano-enzyme. The preparation method comprises the following steps: by taking nitrogen-doped carbon (BANT) as a carrier, loading Fe and Mn diatoms, synthesizing a novel nano material FeMnDA / BCNT diatomic nano-enzyme, extracting a macrophage membrane from natural macrophages, and coating the FeMnDA / BCNT nano material with the macrophage membrane to finally form M2 type macrophage membrane coated nano-particles, namely [MM] FeMnDA / BCNT. The [MM] FeMnDA / BCNT nano-enzyme prepared by the invention has good biological safety and simulated SOD and CAT enzyme activity, and the expression of inflammatory factors is reduced by removing excessive ROS (reactive oxygen species) in the cartilage cells induced by H2O2, so that the damage of oxidative stress to the cartilage cells is inhibited. The [MM] FeMnDA / BCNT nano-enzyme is not used for treating OA yet, so that a scientific basis is provided for further application and expansion of the diatomic nano-enzyme, and an effective strategy and a new thought are provided for treating osteoarthritis and chronic diseases related to oxidative stress in the future.

The invention provides an organ chip unit for simulating the pathogenesis of rheumatoid arthritis, and belongs to the technical field of cell culture. The chip comprises a top-layer chip, a bottom-layer chip and a middle microporous membrane, the top-layer chip and the bottom-layer chip are separated by the middle microporous membrane, and the middle microporous membrane is used for enabling cells in a cell culture space of the top-layer chip and cells in a cell culture space of the bottom-layer chip not to be in direct contact, but interaction of signal molecules and substance exchange can be carried out; synovial cells and cartilage cells are injected into the bottom-layer chip or the top-layer chip to be cultured. According to the invention, multi-cell three-dimensional co-culture can be realized. Through the additional stress stimulation co-culture part, the influence of pressure or tension on the cell environment can be judged, and the actual condition that rheumatoid arthritis joints receive pressure or tension for a long time can be simulated. The synovial cells and the cartilage cells are used, so that the structure of the inflammation joint in a living body can be truly reflected, and the interaction among various cell tissues can be reflected.

The invention discloses a micro-fluidic chip system for simulating a cartilage mechanical loading environment, and belongs to the technical field of biological micro-fluidic chips. A micro-fluidic chip and a peripheral system are designed according to a biomechanical principle and a micro-fluidic chip technology, and a real cartilage microstructure and a mechanical environment can be simulated. The micro-fluidic chip simulates a real cartilage structure and is used for providing internal synovial fluid pressure. The peripheral system provides a controllable external mechanical environment, internal synovial fluid pressure and external three-dimensional mechanical loading of the chip are controlled through PID feedback, a platform is provided for researching a synergistic effect mechanism of the internal synovial fluid pressure and the external three-dimensional mechanical loading, and the peripheral system can also observe diffusion movement of internal nano-particles in the mechanical environment in real time. The system provides an experimental platform for researching the influence and mechanism of mechanical stimulation on OA cartilage cell functions, avoids the defects of ethical problems and individual differences in previous animal experiments, is simpler and more convenient to operate, lower in cost and shorter in experimental period, and provides a scientific basis for researching the influence of mechanical loading on cartilage repair.

The invention provides electroactive nano short fibers for osteoarthritis cartilage repair and a preparation method thereof, the preparation method comprises the following steps: preparing a nano fiber membrane from a composite material of gelatin, polylactic acid and glycine crystals through electrostatic spinning, and preparing the obtained nano fiber membrane into short fibers; performing freeze drying on the short fibers, and then performing heating treatment to obtain a short fiber stent; the short fiber support is smashed and reacts with graphene oxide, modified graphene oxide short fibers are obtained, and then the modified graphene oxide short fibers are soaked in a mixed solution of alkaline dopamine and phosphatidylcholine for a reaction. The electroactive nano short fiber provided by the invention is low in friction coefficient, the expression quantity of Sirt1 in OA cartilage cells can be remarkably increased under ultrasonic stimulation, the ATP (adenosine triphosphate) productivity can be remarkably improved, release of inflammatory factors and degradation of extracellular matrixes can be inhibited, and meanwhile, a guarantee is provided for updating of a hydrated lubricating layer.

The invention relates to a cartilage stem cell for promoting joint repair and application thereof. In particular, the present invention provides a cellular preparation for alleviating or treating osteoarthritis. The cell line comprises Procr-positive pluripotent stem cells. The novel cartilage stem cell provided by the invention can be differentiated in vivo to generate downstream cartilage cells, and cartilage repair in osteoarthritis injury is promoted; it is prompted that selection of Procr positive cartilage stem cells for cell treatment will be a more effective treatment method. And the Procr protein can be used as a new target of the medicine for treating osteoarthritis.

The invention discloses a drug-guided anti-inflammatory enhanced mesenchymal stem cell preparation as well as a preparation method and application thereof. By constructing the medicine-loaded mesenchymal stem cell preparation, the immunosuppression capacity of the mesenchymal stem cells is improved, the angiogenesis capacity of the mesenchymal stem cells is reduced, meanwhile, by releasing the glucocorticoid medicine at a low dosage, the side effect of single administration is avoided, M2 type macrophage polarization is induced, the inflammatory state of cartilage cells is improved, and the effect of treating the cartilage cells is achieved. The curative effect of the mesenchymal stem cells on osteoarthritis is improved in multiple aspects, and the mesenchymal stem cells are proved in animal bodies. The drug-guided anti-inflammatory enhanced mesenchymal stem cell preparation provides a new thought for the treatment of osteoarthritis.

The invention discloses preparation and application of a biological material for targeted therapy of osteoarthritis, and the preparation method comprises the following steps: according to a phage screening principle, after phages are combined with cartilage cells, washing off phages which are not combined with the cartilage cells, are not firmly combined with the cartilage cells and are non-specifically combined with the cartilage cells; eluting the specifically bound bacteriophage with glycine acid; after the specific binding bacteriophage is amplified by escherichia coli, screening is carried out by utilizing a bacteriophage display technology and cartilage cells; the preparation method comprises the following steps: preparing a small-size porous microgel loaded drug by adopting a multiple Pickering emulsion template method; and grafting the screened cartilage targeting peptide to the surface of the porous hydrogel microsphere to obtain the HS-SP microsphere. According to the invention, the problem of OA targeting can be effectively solved by adopting a phage display technology, and a drug is precisely targeted and delivered to an OA lesion region; the porous hydrogel microspheres with small particle size are prepared by adopting a multiple Pickering emulsion method, so that the use of a large number of surfactants is avoided, and the efficient loading and release of drugs can be realized.

The invention discloses a tissue engineering integrated trachea regeneration construction method based on a frame structure scaffold and application. A multi-tissue-structure integrated gas pipe is constructed based on a 3D printing bioelastomer frame structure support, and the complex structure and physiological functions of a natural gas pipe are highly simulated. The preparation method comprises the following steps: firstly, by utilizing the characteristic that temperature-sensitive hydrogel Pluronic F-127 becomes semi-solid gel at 37 DEG C and is liquefied at a low temperature of 4 DEG C, and by adopting a strategy that the temperature-sensitive hydrogel is sacrificed after occupying, the patterned distribution of chondrocytes and fibroblasts on a two-dimensional plane is realized; secondly, simulating a three-dimensional tubular trachea structure through a 2D-3D (two-dimensional to three-dimensional) rolling assembly strategy, and realizing the ordered regeneration of C-shaped cartilage ring / O-shaped fiber ring alternate distribution and axial strip-shaped fiber bundle triple structures under the skin of the nude mouse. And finally, successfully extracting the airway mucosa epithelium, separating and amplifying enough airway basal cells in vitro, and loading the airway basal cells to the inner wall of the regenerated trachea by utilizing the photo-crosslinking hydrogel, thereby realizing the function simulation of the airway epithelium and enhancing the physiological functionality.

The invention provides a combined use of a hyaluronic acid-bilirubin conjugate and honokiol in preparation of a medicine for treating osteoarthritis. The invention provides a honokiol-loaded nanoparticle, which is characterized in that a hyaluronic acid-bilirubin conjugate and honokiol are used as raw materials to prepare a nanoparticle HKL-PNPs, and then the nanoparticle HKL-PNPs is modified by a mitochondrial targeting peptide to obtain the honokiol-loaded nanoparticle HKL-MPNPs. The invention also provides a bionic nanoparticle for treating bone joints. The bionic nanoparticle is prepared by wrapping the honokiol-loaded nanoparticle with an engineered cartilage cell membrane (ECM). According to the invention, cartilage cells with high expression of IL-1R2 are obtained through a gene editing method for the first time, and mitochondrial targeted nanoparticle cores loaded with Sirtuin-3 agonists are coated by cell membranes of the cells, so that the bionic nano preparation is obtained and is used for treating OA. The prepared bionic nano preparation not only can neutralize IL-1beta mediated inflammatory reaction, but also can recover Sirtuin-3 mediated mitochondrial function imbalance, so that the result of synergistic treatment of OA is achieved.

The invention belongs to the technical field of medical treatment, and discloses an osteoarthritis rehabilitation auxiliary device and system, and the device comprises an intelligent vibration treatment module, a pressure sensor and biological feedback module, an intelligent control and APP remote management module, a pressure sensor, an accelerometer, and a myoelectricity sensor. The joint pain is relieved, vibration can stimulate sensory nerves, pain signal transmission is reduced, and the pain degree is reduced. Vibration promotes secretion of joint fluid, friction is reduced, and the range of motion is improved. Low-frequency vibration stimulates the activity of cartilage cells, and cartilage degeneration is slowed down. And a biological feedback system is intelligently regulated and controlled, so that the treatment accuracy is improved. Non-invasive treatment is achieved, and no side effect exists; gastrointestinal discomfort caused by drug treatment or high cost of long-term physical treatment is avoided. Remote monitoring is achieved, compliance is improved, a patient can be treated at home and does not need to see a doctor frequently, and medical cost is reduced.

The invention relates to the technical field of cartilage construction, in particular to an injectable tissue engineering cartilage in-vitro construction method which comprises the following steps: S1, micro-ecological cartilage environment simulation design; s2, self-assembly of the stent and space-time guidance; and S3, constructing a reversible tissue-degradation platform. A tissue engineering system highly simulating a natural cartilage microenvironment is constructed through the technology of combining bionic design and dynamic regulation and control, the proliferation activity and functional differentiation efficiency of cartilage cells can be effectively improved, and meanwhile, by means of intelligent support design and a growth factor delivery system, the tissue engineering system can be applied to the field of cartilage tissue engineering. Accurate release of factors and cell navigation are realized; the dynamic repair technology is combined with the non-contact mechanical stimulation and immune regulation strategy, it is ensured that transplanted tissue can be continuously generated and fully integrated with host tissue, the problems of immunological rejection or insufficient stent degradation and the like are solved, the success rate and long-term stability of cartilage regeneration are remarkably improved, and the method has good clinical application prospects.

The invention relates to the technical field of biomedicine, and provides an efficient separation and culture method of rat meniscus fibrocartilage cells. The yield and activity of the meniscus fibrocartilage cells are improved through a step-by-step enzyme digestion technology, the primary cell adherence time is shortened to 4-6 h by optimizing culture conditions, the breakthrough of sufficiently extracting the meniscus fibrocartilage cells from a single rat is achieved, the use amount of experimental animals is greatly reduced, and the cost is reduced. Meanwhile, it is ensured that the cell activity and proliferation capacity meet the requirements of subsequent passage and functional research, and the method has remarkable advantages in the aspects of ethical compliance, cost control and experimental efficiency.

The invention discloses novel cascaded targeted selenium-based hydrogel microspheres for multi-stage antioxidant treatment of osteoarthritis and a preparation method of the novel cascaded targeted selenium-based hydrogel microspheres. The preparation method comprises the following steps: step S1, preparing an LAP solution; s2, preparing a hydrogel precursor solution; s3, preparing a hydrogel oil phase for later use; step S4, preparing hydrogel microspheres with a porous structure; and step S5, preparing the hydrogel microspheres (AHAMA-HMs) loaded with the novel selenium nanoparticles (HA-SeNPs). The AHAMA hydrogel microspheres loaded with the HA-SeNPs can enter an articular cavity through a 5mL injector, and the cartilage is adhered and abraded in a targeted manner by virtue of a Schiff base reaction of-CHO on the surface of the microspheres and-NH2 on the surface of the cartilage, so that micron-level targeting is realized; then, HA-SeNPs released from the AHAMA-HMs is combined with CD44 on the surface of the abraded cartilage cell through hyaluronic acid (HA), so that nano-scale targeting is realized. Subsequently, after entering the cartilage cells, the HA-SeNPs can participate in removal of excess reactive oxygen species (ROS) in the cells in multiple stages, so that multi-stage antioxidant treatment is realized, and further osteoarthritis is treated.

The invention discloses a dihydro-beta-agilawood furan type sesquiterpenoids compound as shown in a formula (I) as well as a preparation method and application thereof. The compound provided by the invention can promote cartilage cell proliferation and reverse mRNA expression of symbolic genes Col2A1 and SOX-9 in cartilage cells under inflammation induction; meanwhile, the expression of inflammation-induced cartilage cell matrix metalloproteinase-13mRNA can be down-regulated, the degradation of cartilage cell extracellular matrix can be inhibited, and the cartilage cell metalloproteinase-13mRNA can be used for preparing products for preventing and / or treating diseases or symptoms caused by abnormal cartilage cell activity and / or improving bone and joint movement functions. # imgabs0 #

The invention relates to polyglutamic acid bio-ink with antibacterial and antioxidant functions as well as a preparation method and application of the polyglutamic acid bio-ink. The method comprises the following steps: firstly, degrading high-molecular-weight gamma-polyglutamic acid at a specific high temperature to obtain degraded gamma-polyglutamic acid (d gamma-PGA) which is low in viscosity and suitable for printing; then, the gamma-polyglutamic acid (d gamma-PGAGMA) is reacted with a methacrylic acid acylation reagent, and the photo-crosslinkable gamma-polyglutamic acid (d gamma-PGAGMA) degraded by methylacryloylation is prepared; and finally, mixing the dgamma-PGAGMA, a photoinitiator and the living cells to obtain the biological ink. The hydrogel is suitable for 3D biological printing and can be formed at will, and the hydrogel formed through printing has the effects of inhibiting bacteria survival and resisting oxidation and can promote growth of cartilage cells and regeneration of cartilage tissue.

The invention provides a biological liquid medicine for promoting cartilage tissue regeneration by being injected into knee joints. The biological liquid medicine comprises the following components: 3%-4% of modified xanthan gum, 1.5%-2.5% of chitosan lactate wrapped by lipidosome, 0.2%-0.6% of strontium bromide, 1%-2% of disodium hydrogen phosphate, 1%-2% of sodium dihydrogen phosphate and the balance of water for injection to 100%, the modified xanthan gum is carboxymethylated xanthan gum. According to the biological liquid medicine, modified xanthan gum, chitosan lactate wrapped by lipidosome and strontium bromide are added, long-acting retention of the modified xanthan gum provides a stable release environment for the lipidosome and the strontium bromide, the lipidosome accurately delivers the chitosan lactate to an injured part, and the lipidosome and the strontium bromide act on ions to strengthen cartilage cell proliferation and matrix secretion, so that the cartilage cell proliferation and matrix secretion are enhanced; the three components are combined to improve the cartilage tissue damage repair degree of the knee joint and improve the cartilage tissue regeneration effect.

The invention provides recombinant II-type collagen as well as a preparation method and application thereof, and belongs to the technical field of bioengineering. The recombinant II-type collagen monomer is obtained by splicing Gly-X-Y repetitive gene sequences of multiple sections of triple helix regions in human II-type collagen, immunodominant epitopes, unstable amino acid sequence 3 conjunctions and glycosylation sites are avoided during selection, then the recombinant II-type collagen monomer is repeated for multiple times, and the recombinant II-type collagen monomer is obtained. The recombinant II type collagen with the molecular weight of 40-50 kDa is obtained; the recombinant II-type collagen is beneficial to expression of pichia pastoris, has the effects of promoting adhesion and migration of cartilage cells, can also promote differentiation into cartilage cells, and has good practicability.

The invention belongs to the technical field of organic-inorganic compounding, and aims to solve the problem of treatment of temporomandibular joint osteoarthritis (TMJOA). Magnesium-gallic acid metal organic framework (Mg-GA MOF) particles are synthesized through a hydrothermal method, hyaluronic acid is subjected to hydroformylation and double-bond modification to obtain OHAMA, and the magnesium-gallic acid metal organic framework (Mg-GA MOF) particles and the OHAMA particles are compounded to prepare the MOF-Gel composite hydrogel. The hydrogel is injectable and high in tissue retention rate, has dual effects of inhibiting pyroptosis and promoting regeneration on condylar cartilage cells, can effectively promote cartilage repair, shows a good effect in TMJOA treatment, and has a wide application prospect.

The invention relates to a functionalized cartilage tissue repair material as well as a preparation method and application thereof, and belongs to the technical field of tissue engineering. The invention aims to solve the problems that the existing decalcified bone matrix is insufficient in cartilage induction in cartilage repair, and is easy to cause blood vessel ingrowth and fibrosis repair. The preparation method comprises the following steps: inoculating cartilage cells on a three-dimensional porous frame structure carrier, carrying out in-vitro dynamic pre-culture to enable the cartilage cells to secrete an extracellular matrix, and carrying out decellularization treatment to obtain the functionalized cartilage tissue repair material retaining the cartilage specific matrix. The material has a suitable pore structure and abundant II-type collagen deposition, can effectively promote chondrocyte differentiation and hyaline cartilage regeneration, inhibits blood vessel ingrowth, maintains a cartilage vascular-free microenvironment, significantly improves the structure and function reconstruction effect of cartilage defect repair, and has good biocompatibility and clinical transformation potential.

The invention relates to the technical field of biomedical materials, and particularly provides a bone joint biological super-lubricating copolymer and a preparation method thereof. The preparation method comprises the following steps: modifying dopamine by using methacrylic anhydride to prepare dopamine methacrylamide, and carrying out free radical polymerization on poly (2-ethyl-2-oxazoline) which has good hydrophilicity and hydration lubricating property and has positive charges and dopamine methacrylamide with adhesion property to form the super-lubricating copolymer. The super-lubricity copolymer with positive charges has good cell affinity, can promote attachment, growth and differentiation of cartilage cells, and is beneficial to tissue repair and regeneration. The super-lubricating copolymer with positive charges has high hydrophilicity, excellent hydration capability and lubricating property, can be attached to the joint surface, absorbs surrounding water molecules to form a hydration layer and effectively reduces surface friction and abrasion, free radicals can be effectively removed through the unique adhesion property of the copolymer and a phenol structure, and the anti-inflammatory effect is achieved.

The application provides yak cartilage peptide with a function of repairing cartilage injury, and a preparation method and application thereof, and belongs to the technical field of active peptides. The application selects crescent bones of yaks as raw materials, adopts papain and trypsin for complex enzymolysis, and combines with ultrasonic-assisted enzymolysis technology, so that the enzymolysis efficiency and peptide yield are greatly improved. The yak cartilage peptide prepared by the application is proved to have good antioxidant activity and the effect of promoting cartilage cell proliferation through in-vitro cell experiments, and the effect is significantly better than that of traditional enzymolysis methods and commercial pig bone peptide, fetal bovine bone peptide and other products. Therefore, the yak cartilage peptide prepared by the application can be used for repairing cartilage injury, treating cartilage degeneration and improving symptoms related to osteoarthritis.

The present application relates to the technical field of cell and tissue engineering, and particularly relates to a culture medium and method for preparing hypertrophic cartilage tissue. The hypertrophic cartilage induction culture medium can effectively improve the induction and differentiation efficiency of hypertrophic cartilage cells or tissue, and the prepared hypertrophic cartilage tissue has high biocompatibility and activity and performs excellently in promoting bone defect repair. The culture medium group for inducing iPSC differentiation to prepare hypertrophic cartilage cell membrane pieces can accurately and efficiently induce iPSC to differentiate into hypertrophic cartilage cells, and can further form hypertrophic cartilage cell membrane pieces; the hypertrophic cartilage cell membrane pieces can quickly induce the regeneration of osteogenic centers, and provide a solution for treating large-area bone defects, and have good application prospect.

The utility model provides a composite cartilage scaffold. The composite cartilage scaffold comprises a cartilage scaffold layer, a calcification scaffold layer and a subchondral bone scaffold layer which are sequentially stacked and arranged from top to bottom, a plurality of first immersion channels communicated with each other are arranged in the cartilage stent layer and the calcification stent layer, a plurality of first immersion holes are formed in the top of the cartilage stent layer, a plurality of second immersion holes are formed in the side wall of the calcification stent layer, and the first immersion holes and the second immersion holes are communicated with the first immersion channels. Before an operation, the cartilage scaffold layer and the calcification scaffold layer are soaked with cartilage cells, and the cartilage cells are rapidly immersed into the first immersion channel through the first immersion hole and the second immersion hole, so that the cartilage scaffold layer and the calcification scaffold layer can absorb more sufficient cartilage cells, and the cartilage cell content of the scaffold is increased; the secretion of cartilage extracellular matrix is better promoted, the extension of cartilage cells and the coupling between the cartilage cells and in-situ cartilage are facilitated, and the repair of cartilage injury is further promoted.

The application provides a preparation method and application of a pH / ROS dual-responsive functionalized biomimetic nanomaterial loaded with mannose. The nanomaterial takes a pH / ROS dual-responsive material as a carrier and simultaneously loads a mannose drug. The prepared nanoparticles are wrapped with functionalized macrophage membranes on the surface of the nanoparticles by a lipid extruder to achieve targeted drug delivery. The functionalized biomimetic nanoparticles provided by the application can rapidly release mannose in an acidic and / or high active oxygen microenvironment, thereby effectively promoting the generation of cartilage cell extracellular matrix. The application provides a promising strategy for targeted treatment of intervertebral disc degeneration induced by cartilage injury.

The invention discloses a recombinant II-type collagen for promoting cartilage repair as well as a preparation method and application of the recombinant II-type collagen. The amino acid sequence of the recombinant II-type collagen is constructed by repeating the amino acid sequence as shown in SEQ ID NO: 1 for multiple times, and the number of repeating times is larger than or equal to 2 and smaller than or equal to 20. The recombinant II type collagen disclosed by the invention has good activity of promoting cartilage cell proliferation, and particularly, the recombinant II type collagen of the recombinant II type collagen is superior to full-length II type collagen, and can be used for promoting cartilage repair. The recombinant II-type collagen has 100% sequence identity with human II-type collagen, strictly follows a Gly-X-Y repetitive sequence mode, does not introduce heterologous amino acid residues, and does not have rejection and sensitization risks.

The invention provides a compound bone-strengthening composition containing astragalus polysaccharide and polypeptide as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The composition is prepared by compounding astragalus polysaccharide and collagen peptide, the astragalus polysaccharide is prepared by combining ultrasonic extraction of a high-voltage pulsed electric field and a deep-eutectic solvent, and the collagen peptide is prepared by compound microbial fermentation, step-by-step enzymolysis and purification. The synergistic effect of the astragalus polysaccharide and the collagen peptide is remarkable, the astragalus polysaccharide resists inflammation and oxidation and optimizes the repairing microenvironment, and the collagen peptide provides a repairing raw material and promotes tissue regeneration. The composition disclosed by the invention can be used for efficiently promoting joint movement injury repair, improving cartilage cell activity, removing active oxygen and inhibiting secretion of inflammatory factors. Cell experiments and zebra fish animal experiments show that the composition is excellent in repairing effect, and bone strengthening products prepared on the basis of the composition also have good repairing performance and have important clinical value and market prospects.

The invention discloses a bone-derived collagen peptide with a joint protection effect and a preparation method thereof, and belongs to the technical field of collagen peptide preparation, the preparation method comprises the following steps: S1, cleaning and crushing fresh fish bones, and performing impurity protein removal treatment on fish bone powder by using a 0.1 M NaOH solution to obtain pretreated bone powder; s2, mixing the pretreated bone meal with 0.5-1.5 mol / L hydrochloric acid according to a solid-to-liquid ratio of 1: 5, and carrying out a decalcification reaction for 2-4 hours to obtain decalcified bone collagen; s3, performing sequential enzymolysis on the decalcified ossein by adopting pepsin and trypsin to obtain enzymatic hydrolysate; and carrying out inactivation, purification, vacuum concentration and freeze drying on the enzymatic hydrolysate to obtain the collagen peptide. According to the collagen peptide and the preparation method, the characteristic amino acid content of the prepared collagen peptide is increased by 40% or above compared with that of a traditional process, it is ensured that the collagen peptide is combined with a cartilage cell receptor in a targeted mode and inhibits secretion of inflammatory factors, and the inhibition rate reaches 58-68%; at a concentration of 500 [mu] g / mL, there is no toxicity to HaCaT cells (IC50gt; and the traditional Chinese medicine composition has no toxic or side effect and no skin irritation.

The application discloses a kind of targeted delivery molecules, particle and its preparation method, application, comprising the following steps: step 1: to the polyethylene glycol molecule A solution containing maleimide group and hydrophobic group is added dropwise with thiol-containing polypeptide targeting factor B solution, stir and react fully;Wherein the amount-of-substance ratio of A and B is 1:1;Step 2: the solution obtained in step 1 is dialyzed, freeze-dried to obtain the required targeted delivery molecules;The targeted delivery molecules prepared by the application have cartilage cell-specific polypeptide targeting factor at one end, and can target cartilage tissue.The hydrophobic group at the other end can be inserted into the hydrophobic lipid bilayer by physical action.The polypeptide targeting factor is assembled on the surface of cell, cell exosome, liposome and other vesicles composed of lipid bilayer, which has the effect of treating osteoarthritis.The above-mentioned cells and vesicles can be targeted to deliver the contents to cartilage, and have good application prospect in the treatment of osteoarthritis.

A tissue-derived implant including: a plurality of decellularized extracellular cartilage particles, wherein each of the plurality of cartilage extracellular particles comprise one or more magnetic particles and mesenchymal stem cells and method of preparation and method use thereof. The tissue-derived implant can be guided to the site of a cartilage defect after administration by use of a magnetic field.