304 results about "Cartilage cells" patented technology

Methods and devices for the regulation of type II collagen gene expression in cartilage cells via the application of specific and selective fields generated by specific and selective electric and electromagnetic signals in the treatment of diseased or injured articular cartilage. By gene expression is meant the up regulation or down regulation of the process whereby specific portions (genes) of the human genome (DNA) are transcribed into mRNA and subsequently translated into protein. Methods and devices are provided for the targeted treatment of injured or diseased cartilage tissue that include generating specific and selective electric and electromagnetic signals that generate specific and selective fields optimized for type II collagen gene expression and exposing cartilage tissue to the specific and selective fields generated by specific and selective signals so as to regulate type II collagen gene expression in such cartilage tissue. The resulting methods and devices are useful for the targeted treatment of osteoarthritis, rheumatoid arthritis, cartilage injury, and cartilage defects.

An allograft osteochondral plug is combined with a mixture that includes freeze-milled cartilage particles, and such combination is used to repair defects in articular cartilage. The plug includes an subchondral bone portion and an integral overlying cartilage cap which is treated to remove cellular debris and proteoglycans. At least a portion of the plug has a lateral dimension selected to form an interference fit against a tissue layer exposed as a result of a bore formed in a defect area in articular cartilage of a host. The cartilage particle mixture is placed adjacent at least a portion of the plug for promoting cartilage cell migration into (i.e., from the adjacent host cartilage) and proliferation in the bore, and for enhancing tissue integration between the plug and patient (i.e., host) tissue when the plug is inserted into the bore. Methods for surgical implantation of the plug into a patient are also disclosed.

The articular cartilage according to the invention is made of pure cartilage and is provided with incisions (12) on the surface facing the bone. The cartilage cells are preferably seeded on the surface provided with incisions (12). The method for producing the articular cartilage comprises the step of collecting cartilage from joints, wherein pure cartilage is collected without bone, and incisions are made on the surface of the cartilage intended to face the bone. It is preferably fresh frozen until use. The device for harvesting articular cartilage, comprises handle and cutting blade, wherein the cutting blade (4) is curvilinear and is provided with spacer elements (5), meanwhile the device for producing incisions in articular cartilages comprises handle (2) and a bridge (3) connected to said handle (2) and being provided with one or more cutting blade(s) (4). During the method for applying the articular cartilage the articular cartilage is fixed by thin surgical yarn stitches, by fibrin glue or by small anchors (FIG. 8).

The invention discloses a three-dimensional porous cartilage extracellular matrix sponge scaffold made of natural cartilage, which can compound cells further to construct tissue engineered cartilage, and can be used for clinically repairing cartilage defects. In the invention, conditions which can fully perform cell extraction and form a porous scaffold matrix are provided by processing cartilage into cartilage microfilaments, and then the cell extraction and solidification and/or strengthening treatment are performed to obtain the three-dimensional porous cartilage extracellular matrix sponge scaffold which is completely decellurized. The antigenicity and cell components are removed in the natural cartilage, and an extracellular matrix component of the cartilage is retained to obtain the scaffold with appropriate pore diameter and porosity, suitable degradation rate, good biocompatibility and certain biomechanical strength. The scaffold has the advantages of broad material sources, low cost, simple and feasible preparation technology, and good repetitiveness; and the scaffold can be widely applied in the field of tissue engineering and has good clinical application prospect.

The present invention proposes formative agent of protein complex, in which a polyphenol is useful component, and the agent is useful as gene complex, cell adhesion inhibitor or immune tolerogen. The polyphenol of forming the agent is selected from catechin group consisting of epigallocatechin-gallate, tannic acids, or proanto-dianisidine, a protein of the protein complex is selected from proteins consisting of animal proteins composed of polypeptide chain of peptide-combined amino acids, vegetative proteins, nucleus proteins, glycogen proteins, lipo-proteins and metal proteins, the gene complex comprises by compositing genes by polyphenol catechins in order to introduce genes to cells of animals or human bodies, a cell composed of the cell adhesion inhibitor is selected from cells consisting of an animal cell including a stem cell, skin cell, mucosa cell, hepatocyte, islet cell, neural cell, cartilage cell, endothelial cell, epidermal cell, osteocyte or muscle cell isolated from human or animal organism, or sperm, ovum or fertilized egg of domestic animals or fishes and a tissue or an organ for transplantation of the immune tolerogen is selected from the tissue or the organ consisting of skin, blood vessel, cornea, kidney, heart, liver, umbilical cord, bowels, nerve, lung, placenta or pancreas.

The invention relates to a human amnion mesenchymal stem cell serum-free culture medium and a culture method thereof. The culture medium is formed by adding human serum albumin, human transferrin, human insulin and sodium selenite into a DMEM/F12 basic culture medium. The culture method for the culture medium comprises the following steps of: digesting human amnion by using trypsin, then digesting the human amnion by using collagenase IV and deoxyribonuclease I, and filtering the mixture to obtain single cell suspension; and adding the human serum albumin, the transferrin, the insulin and the sodium selenite into the DMEM/F12 basic culture medium in a ratio of VDMEM to VF12 of 1:1, and putting human amnion mesenchymal stem cells in a 37 DEG C CO2 incubator with saturated humidity and volume fraction of 5 percent under the serum-free condition, wherein culture in vitro and amplification are realized by solution change and transfer of culture, potentiality of multi-direction differentiation is maintained, and the amplified cells can be induced in vitro to form cartilage cells, osteoblasts and adipocytes. The culture medium and the culture method have the characteristics of no other animal sources, wide source and no limitation of ethics.

This invention relates to human adipose tissue-derived multipotent adult stem cells. More particularly, the invention relates to human adipose tissue-derived multipotent stem cells, which can be maintained in an undifferentiated state for a long period of time by forming spheres and have high proliferation rates, as well as methods for isolating and maintaining the adult stem cells, and methods for differentiating the multipotent adult stem cells into nerve cells, fat cells, cartilage cells, osteogenic cells and insulin-releasing pancreatic beta-cells. Also, the invention relates to cellular therapeutic agents for treating osteoarthritis, osteoporosis and diabetes and for forming breast tissue, which contain the differentiated cells or the adult stem cells. Although the multipotent stem cells are adult stem cells, they have the ability to differentiate into osteogenic cells, nerve cells, astrocytes, fat cells, chrondrogenic cells or insulin-releasing pancreatic beta-cells, and so are effective in treating osteoporosis, osteoarthritis, nerve disease, diabetes, etc. Also, the stem cells form spheres in a serum-free medium containing CORM-2, and thus can be maintained in an undifferentiated state for a long period of time. Also, the stem cells have very high proliferation rates. Accordingly, the stem cells are useful as cellular therapeutic agents.

There is provided a technology for simple three-dimensional culturing of cartilage cells. The technology includes a method for culturing cartilage cells which comprises seeding cartilage cells 1 on a plurality of projected portions 21 of a culturing substrate 2 having a culture surface on which the projected portions 21 having an equivalent diameter and an interval which are smaller than the equivalent diameter of the cartilage cells 1 to be cultured, placing the culture substrate 2 having the cartilage cells 1 in a culture vessel, and culturing the cartilage cells to form aggregates of cartilage cells.

Methods and devices for the regulation of matrix metalloproteinase gene expression in cartilage cells via the application of fields generated by specific and selective electric and electromagnetic signals in the treatment of diseased or injured articular cartilage. By gene expression is meant the up-regulation or down-regulation of the process whereby specific portions (genes) of the human genome (DNA) are transcribed into mRNA and subsequently translated into protein. Methods and devices are provided for the targeted treatment of injured or diseased cartilage tissue that include generating specific and selective electric and electromagnetic signals that generate fields optimized for reduction of matrix metalloproteinase gene expression and exposing cartilage tissue to the fields generated by specific and selective signals so as to regulate matrix metalloproteinase gene expression in such cartilage tissue. The resulting methods and devices are useful for the targeted treatment of osteoarthritis, rheumatoid arthritis, cartilage injury, cartilage defects, and tumor metastasis.

The invention provides an integrated bone-cartilage repair scaffold. The integrated bone-cartilage repair scaffold is of an integrated structure composed of a subchondral bone repair layer, a middlelayer and a cartilage repair layer, wherein the subchondral bone repair layer is fashioned porous calcium phosphate biological ceramic, the middle layer is sulfydryl-hyaluronic acid hydrogel, the cartilage repair layer is composed of I-type collagen hydrogel and chondrocytes or mesenchymal stem cells, and the middle layer is located between the subchondral bone repair layer and the cartilage repair layer and in a porous structure of the fashioned porous calcium phosphate biological ceramic to separate the subchondral bone repair layer from the cartilage repair layer. The invention further provides a preparation method of the integrated bone-cartilage repair scaffold. The repair scaffold can prevent blood vessels from invading a cartilage layer, so that the tightness and the stability of connection between a bone layer and the cartilage layer are improved, biological bonding of the bone layer and the cartilage layer is achieved, and thus a good repair effect is achieved.

The invention relates to a biological material for repairing meniscus tear and a preparation method for the biological material. The material consists of an acellular matrix membrane on the inner layer, a porous structure collagen matrix membrane on the middle layer, a cell layer formed by cartilage cells on the outer layer and a cell sheet. The repair material has certain elasticity and toughness and is high in biological activity; the cartilage cells can secrete cell factors per se, and nutritional ingredients can be directly obtained through articular cavity synovia, and thus, the establishment of communication link between tissue cells at both ends of the tear during the meniscus tear process is accelerated; the immigration and the infusion of the cells in the material and autologous tissue cells are promoted; the tissue regeneration and functional reconstruction time is shortened; and the ideal novel material and the preparation method for the material are provided for clinically repairing the damage of the meniscus tear (including blood supply zones), especially the tissue regeneration and the functional reconstruction of no-blood zones and low-blood zones.

The invention provides a composition and a method for inducing mesenchymal stem cells to be differentiated to cartilage cells. The composition comprises 20-80 uM of L-ascorbic acid-2-phosphate, 2-10 ng/ml of TGF beta 3, 50-150 nM of dexamethasone, 10-50 ug/ml of vitamin C, 10-50 ug/ml of proline, 0.5-5 ug/ml of indometacin, 1xITS+1premix and 0.5-10 ug/ml of chlormadinone ester. The combination when being used as an inducer of a culture medium can improve differentiation activity and differentiation directionality.

The invention belongs to the field of cartilage tissue repair and tissue engineering and provides a cartilage complex of a tissue engineering bone and a method for preparing the same. The preparation method comprises the following steps of: performing marrow removal, degreasing and layered calcium-removal of cancellous allograft bones or xenogeneic cancellous bones to obtain the cartilage framework of tissue engineering bones, wherein the upper layer of the cartilage framework is completely decalcified and only the surface of the lower layer is decalcified; and using the framework as a primary framework, adding gel-type matters, such as fibrinogens or alginic acid, serving as a secondary framework and a distribution carrier to obtain the cartilage complex of the tissue engineering bone. Compared with the conventional tissue engineering bone cartilage, the cartilage complex of the invention has the advantages of complete natural biomaterials, complete integration, no application of the layered stacking and interface combination technology, high pressure resistant, tensile resistant and shearing resistant properties, uniform and accurate loading of cells, effective enforcement of the differentiation of seeded cells into cartilage cells, easy operation, convenient minimally invasive operation under an arthroscope, and the like.

The invention provides a method for separating and culturing mesenchymal stem cells from an umbilical cord and inducing and differentiating the mesenchymal stem cells into cartilage cells. The method comprises the steps that the umbilical cord is treated; the cells are separated from the umbilical cord and umbilical vessels and subjected to primary cell culture; cell subculture is conducted, and the mesenchymal stem cells are obtained; the mesenchymal stem cells are induced and differentiated into the cartilage cells, wherein in the step that the cells are separated from the umbilical cord and the umbilical vessels, the umbilical cord and the umbilical vessels are digested through digestive juice. By means of the method, the mesenchymal stem cells can be separated from the umbilical cord and cultured, and the obtained mesenchymal stem cells are subjected to further induced transformation; the method can be used for mass production of the mesenchymal stem cells and has importance significance in treatment of injured tissue which can hardly regenerate.

The present invention relates to a medical composite biomaterial. More specifically, the present invention relates to a medical composite biomaterial including collagen and a hyaluronic acid derivative. Also, the present invention relates to a cartilage cell treating agent using the biomaterial and stem cells derived from a mammal umbilical cord. The biomaterial does not cause an immune reaction, has superior durability, and the cartilage cell treating agent comprising the biomaterial and the stem cells enables arthroscopic surgery, thereby reducing pain of the patient and effectively treat of degenerative arthritis and cartilage damage.

The invention relates to a method for inducing differentiation from mesenchymal stem cells to cartilage cells and application of the mesenchymal stem cells in osteoarthritis, and provides a method for preparing umbilical cord mesenchymal stem cells differentiated to the cartilage cells, mesenchymal stem cells differentiated to the cartilage cells and application of the prepared drug prepared by the mesenchymal stem cells in treatment of the osteoarthritis. The method comprises the following steps of: inducing and culturing the mesenchymal stem cell in a 15ml taper-bottom centrifuge tube by using a globule method; and slightly shaking the centrifuge tube for one time every 24 hours to separate cell sphere from the bottom of the tube, wherein the differentiation inducing culture medium is prepared by adding 100mu g/ml of sodium pyruvate, 10ng/ml TGFbeta3, 100nM of dexamethasone, 25mu g/ml of vitamin C, 40mu g/ml of proline and 1*ITS and 1 of premix into a high-glucose dulbecco modified eagle medium (DMEM). A non-bracket cartilage built by the method can be used for repairing damaged articular cartilages.

A tissue engineering cartilage construction method using bone matrix gelatin which comprises, using cartilage cell for isolated culture or base material stem cell for isolated culture to evoke cartilage cell i.e. seed cell, fetching New Zealand rabbit or human embryon os longum and metaphysic trabecular bone to construct bone matrix gel BMG, inoculating the seed cells harvested through isolated culture onto cortex bone or cancellous bone BMG for extracorporal culture.

The invention provides a preparation method of a multifunctional layered joint cartilage support. The multifunctional layered joint cartilage support capable of simulating the biological property andthe mechanical property of natural cartilage is prepared by taking hydrogel as a support material, taking mesenchymal stem cells, cartilage cells and osteoblasts as inducing and developing objects andby adopting a 3D printing technology. The multifunctional layered joint cartilage support can accurately simulate the internal tissue form and the contour of each layer of the natural cartilage and can realize that different layers have different mechanical and biological characteristics. The prepared artificial cartilage support cells are derived from patients, the support serves as a degradablehydrogel material and the rejection reaction after implantation is greatly reduced. Various nutritional substances and growth factors are mixed in the hydrogel support, so that the sustained releaseeffect can be achieved and regulation and control of the growth and development of the artificial cartilage can be realized.

The invention discloses a method for preparing a cartilage derived collagen sponge scaffold and a collagen sponge scaffold prepared by the method. The method comprises the following steps of crushing, removing cells, removing telopeptide, dissolving and crosslinking, and the obtained collagen sponge scaffold has good biocompatibility and extremely low antigenicity; and a homogenized sponge scaffold can be prepared from cartilages of a plurality of individuals from the conspecific animals, has controllable pore diameter and porosity, can provide appropriate growth and propagation environment for cartilage cells and bone mesenchymal stem cells, particularly has good effect of repairing cartilage defect parts, and is more favorable for standardized production and clinical use.

The invention belongs to the field of articular cartilage repair materials, and provides a preparation method and application of high strength and porous structure polyvinyl alcohol-gallotannic acid hydrogel. The hydrogel is a potential articular cartilage replacement material. According to the preparation method, polyvinyl alcohol is adopted as a substrate material, the ice crystal forming ability of a hydrogel precursor solution is improved by introducing gallotannic acid and microbubbles, and meanwhile, the mechanical property of the hydrogel is greatly enhanced due to the intermolecular interaction between the gallotannic acid and the polyvinyl alcohol. The porous structure and high strength hydrogel is prepared at one step, the preparation process is simple, repeatability is high, theobtained hydrogel has good biocompatibility, meets a porous environment for adhesion and proliferation of cartilage cells and the mechanical property similar to natural articular cartilage, and can be applied to the field of articular cartilage replacement materials.

The invention relates to a cartilage preservation solution. Each 1000ml of the preservation solution comprises 20-30mmol of potassium dihydrogen phosphate, 80mmol of histidine or 15mmol of a histidine salt, 75-95mmol of lactobionic acid, 50-70mmol of sucrose, 0.8-1.2mmol of allopurinol, 45-55g of low molecular dextran-40, 45-55mmol of NaCl, 25-35mmol of KCl, 3-7mmol magnesium sulfate, 70-90U of penicillin, 2-4mmol of reduced glutathione, 3-7mmol of adenosine, 2-5g of trehalose, and the balance deionized water, and the pH value of the cartilage preservation solution is 7.33-7.53. The cartilagepreservation solution is packaged, sealed and preserved at 0-4DEG C. By adopting the preservation solution to preserve cartilage tissues, the death speed of cartilage cells is slowed, the activity ofcartilage fibers is maintained, the performance of the cartilage tissues is kept, and the antibiotic property is increased, so the preservation solution has a good preservation effect and a long preservation time on the cartilage tissues, and is in favor of clinic cartilage tissue transplant operations.

The invention relates to a preparation method of a nano micrometer structure coexistence chitosan double-layer support, which belongs to the biology medical material field. The preparation method is characterized in that a phase separating technology is used to prepare a three-dimensional micrometer chitosan support as a basal layer, a high voltage static static spinning technology is used for spinning nano chitosan fiber on the micrometer chitosan surface, and thereby the nano micrometer structure coexistence chitosan double-layer support can be obtained. The prepared double layer support has good mechanical strength and high amount of porosity, and is suitable for adhesion, growth and differentiation of cells. The surface layer chitosan nano fiber is suitable for growth of cartilage cells, the basal layer micrometer chitosan support is suitable for the growth of the osteoblast, and the nano micrometer structure coexistence chitosan double-layer support can be used for restoring joint position of the human body cartilage and hard bone.

The invention provides a biological artificial trachea and a preparation method thereof in combination with a 3D printing technology. Materials adopted for the biological artificial trachea simultaneously have flexibility and a certain mechanical strength, so that physical and mechanical properties of the trachea serving as a hollow organ in the chest are met. The artificial trachea is porous and loosened in structure and favorable for nutrient diffusion and ingrowth of autologous vessels. In addition, seed cells obtained by expansion of airway epithelial cells and cartilage cells of a receptor are printed on the trachea wall, and patients' rejection reaction to cells is avoided. Besides, the seed cells are composed of mature cells in terminal differentiation, the induced differentiation problem and the tumor formation risk caused by the use of stem cells can be avoided. The spatial distribution of the cells is accurately controllable in printing, and the restorative process is accelerated according to the histology regular distribution. Finally, a trachea support can be degraded in vivo, complications caused by the fact that foreign matter remains in vivo are reduced, and the risk of taking out the foreign matter through a second operation is avoided.

The present invention provides an intra-articular injection administration preparation containing trehalose, which is characterized in that the preparation contains the trehalose. The present invention can improve the capabilities of the cartilage cells to adapt to and resistant to the environmental changes through the intra-articular injection administration, at the same time, the trehalose has the significant effects of antioxidant and anti-free radicals, which can alleviate the symptoms of arthritis; in addition, the trehalose also provides the necessary sugar for cartilage repair, so as to repair and reconstruct the cartilage. The present invention is used for improving and treating the rheumatism, rheumatoid arthritis, osteoarthritis, synovitis and/or cartilage injury.

The invention belongs to the field of cartilage tissue engineering. The invention discloses a tissue engineering cartilage composite scaffold, which is constructed based on an extracellular matrix-derived cartilage micro-tissue, and a preparation method of the composite scaffold. Specifically, a cartilage extracellular matrix-derived micro-carrier is prepared from fresh cartilage through such processes as wet pulverization, sieving, decellularizing and the like; and the prepared micro-carrier can be used as a seed cell amplification vector. By virtue of a bioreactor, cartilage cells are rapidly amplified by the micro-carrier and the phenotype of the cartilage cells is kept; and meanwhile, the induced differentiation of stem cells towards the cartilage is also promoted and the cartilage micro-tissue is formed. By filling the cartilage micro-tissue in pores of a three-dimensional porous scaffold which is good in mechanical performance, the tissue engineering cartilage composite scaffold is constructed. The composite scaffold is good in mechanical performance, rich in natural cartilage extracellular matrix components and conducive to the induced differentiation of the stem cells towards the cartilage, and the composite scaffold is capable of providing a good microenvironment for the growth of seed cells and is expected to accelerate the in vivo construction speed of the tissue engineering cartilage.