44 results about "Cartilage cells" patented technology

The invention relates to polyglutamic acid bio-ink with antibacterial and antioxidant functions as well as a preparation method and application of the polyglutamic acid bio-ink. The method comprises the following steps: firstly, degrading high-molecular-weight gamma-polyglutamic acid at a specific high temperature to obtain degraded gamma-polyglutamic acid (d gamma-PGA) which is low in viscosity and suitable for printing; then, the gamma-polyglutamic acid (d gamma-PGAGMA) is reacted with a methacrylic acid acylation reagent, and the photo-crosslinkable gamma-polyglutamic acid (d gamma-PGAGMA) degraded by methylacryloylation is prepared; and finally, mixing the dgamma-PGAGMA, a photoinitiator and the living cells to obtain the biological ink. The hydrogel is suitable for 3D biological printing and can be formed at will, and the hydrogel formed through printing has the effects of inhibiting bacteria survival and resisting oxidation and can promote growth of cartilage cells and regeneration of cartilage tissue.

The invention provides a biological liquid medicine for promoting cartilage tissue regeneration by being injected into knee joints. The biological liquid medicine comprises the following components: 3%-4% of modified xanthan gum, 1.5%-2.5% of chitosan lactate wrapped by lipidosome, 0.2%-0.6% of strontium bromide, 1%-2% of disodium hydrogen phosphate, 1%-2% of sodium dihydrogen phosphate and the balance of water for injection to 100%, the modified xanthan gum is carboxymethylated xanthan gum. According to the biological liquid medicine, modified xanthan gum, chitosan lactate wrapped by lipidosome and strontium bromide are added, long-acting retention of the modified xanthan gum provides a stable release environment for the lipidosome and the strontium bromide, the lipidosome accurately delivers the chitosan lactate to an injured part, and the lipidosome and the strontium bromide act on ions to strengthen cartilage cell proliferation and matrix secretion, so that the cartilage cell proliferation and matrix secretion are enhanced; the three components are combined to improve the cartilage tissue damage repair degree of the knee joint and improve the cartilage tissue regeneration effect.

The invention provides recombinant II-type collagen as well as a preparation method and application thereof, and belongs to the technical field of bioengineering. The recombinant II-type collagen monomer is obtained by splicing Gly-X-Y repetitive gene sequences of multiple sections of triple helix regions in human II-type collagen, immunodominant epitopes, unstable amino acid sequence 3 conjunctions and glycosylation sites are avoided during selection, then the recombinant II-type collagen monomer is repeated for multiple times, and the recombinant II-type collagen monomer is obtained. The recombinant II type collagen with the molecular weight of 40-50 kDa is obtained; the recombinant II-type collagen is beneficial to expression of pichia pastoris, has the effects of promoting adhesion and migration of cartilage cells, can also promote differentiation into cartilage cells, and has good practicability.

The invention belongs to the technical field of organic-inorganic compounding, and aims to solve the problem of treatment of temporomandibular joint osteoarthritis (TMJOA). Magnesium-gallic acid metal organic framework (Mg-GA MOF) particles are synthesized through a hydrothermal method, hyaluronic acid is subjected to hydroformylation and double-bond modification to obtain OHAMA, and the magnesium-gallic acid metal organic framework (Mg-GA MOF) particles and the OHAMA particles are compounded to prepare the MOF-Gel composite hydrogel. The hydrogel is injectable and high in tissue retention rate, has dual effects of inhibiting pyroptosis and promoting regeneration on condylar cartilage cells, can effectively promote cartilage repair, shows a good effect in TMJOA treatment, and has a wide application prospect.

The invention relates to a functionalized cartilage tissue repair material as well as a preparation method and application thereof, and belongs to the technical field of tissue engineering. The invention aims to solve the problems that the existing decalcified bone matrix is insufficient in cartilage induction in cartilage repair, and is easy to cause blood vessel ingrowth and fibrosis repair. The preparation method comprises the following steps: inoculating cartilage cells on a three-dimensional porous frame structure carrier, carrying out in-vitro dynamic pre-culture to enable the cartilage cells to secrete an extracellular matrix, and carrying out decellularization treatment to obtain the functionalized cartilage tissue repair material retaining the cartilage specific matrix. The material has a suitable pore structure and abundant II-type collagen deposition, can effectively promote chondrocyte differentiation and hyaline cartilage regeneration, inhibits blood vessel ingrowth, maintains a cartilage vascular-free microenvironment, significantly improves the structure and function reconstruction effect of cartilage defect repair, and has good biocompatibility and clinical transformation potential.

The application provides yak cartilage peptide with a function of repairing cartilage injury, and a preparation method and application thereof, and belongs to the technical field of active peptides. The application selects crescent bones of yaks as raw materials, adopts papain and trypsin for complex enzymolysis, and combines with ultrasonic-assisted enzymolysis technology, so that the enzymolysis efficiency and peptide yield are greatly improved. The yak cartilage peptide prepared by the application is proved to have good antioxidant activity and the effect of promoting cartilage cell proliferation through in-vitro cell experiments, and the effect is significantly better than that of traditional enzymolysis methods and commercial pig bone peptide, fetal bovine bone peptide and other products. Therefore, the yak cartilage peptide prepared by the application can be used for repairing cartilage injury, treating cartilage degeneration and improving symptoms related to osteoarthritis.

The present application relates to the technical field of cell and tissue engineering, and particularly relates to a culture medium and method for preparing hypertrophic cartilage tissue. The hypertrophic cartilage induction culture medium can effectively improve the induction and differentiation efficiency of hypertrophic cartilage cells or tissue, and the prepared hypertrophic cartilage tissue has high biocompatibility and activity and performs excellently in promoting bone defect repair. The culture medium group for inducing iPSC differentiation to prepare hypertrophic cartilage cell membrane pieces can accurately and efficiently induce iPSC to differentiate into hypertrophic cartilage cells, and can further form hypertrophic cartilage cell membrane pieces; the hypertrophic cartilage cell membrane pieces can quickly induce the regeneration of osteogenic centers, and provide a solution for treating large-area bone defects, and have good application prospect.

The application provides a preparation method and application of a pH / ROS dual-responsive functionalized biomimetic nanomaterial loaded with mannose. The nanomaterial takes a pH / ROS dual-responsive material as a carrier and simultaneously loads a mannose drug. The prepared nanoparticles are wrapped with functionalized macrophage membranes on the surface of the nanoparticles by a lipid extruder to achieve targeted drug delivery. The functionalized biomimetic nanoparticles provided by the application can rapidly release mannose in an acidic and / or high active oxygen microenvironment, thereby effectively promoting the generation of cartilage cell extracellular matrix. The application provides a promising strategy for targeted treatment of intervertebral disc degeneration induced by cartilage injury.

The invention discloses a recombinant II-type collagen for promoting cartilage repair as well as a preparation method and application of the recombinant II-type collagen. The amino acid sequence of the recombinant II-type collagen is constructed by repeating the amino acid sequence as shown in SEQ ID NO: 1 for multiple times, and the number of repeating times is larger than or equal to 2 and smaller than or equal to 20. The recombinant II type collagen disclosed by the invention has good activity of promoting cartilage cell proliferation, and particularly, the recombinant II type collagen of the recombinant II type collagen is superior to full-length II type collagen, and can be used for promoting cartilage repair. The recombinant II-type collagen has 100% sequence identity with human II-type collagen, strictly follows a Gly-X-Y repetitive sequence mode, does not introduce heterologous amino acid residues, and does not have rejection and sensitization risks.

The invention provides a compound bone-strengthening composition containing astragalus polysaccharide and polypeptide as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The composition is prepared by compounding astragalus polysaccharide and collagen peptide, the astragalus polysaccharide is prepared by combining ultrasonic extraction of a high-voltage pulsed electric field and a deep-eutectic solvent, and the collagen peptide is prepared by compound microbial fermentation, step-by-step enzymolysis and purification. The synergistic effect of the astragalus polysaccharide and the collagen peptide is remarkable, the astragalus polysaccharide resists inflammation and oxidation and optimizes the repairing microenvironment, and the collagen peptide provides a repairing raw material and promotes tissue regeneration. The composition disclosed by the invention can be used for efficiently promoting joint movement injury repair, improving cartilage cell activity, removing active oxygen and inhibiting secretion of inflammatory factors. Cell experiments and zebra fish animal experiments show that the composition is excellent in repairing effect, and bone strengthening products prepared on the basis of the composition also have good repairing performance and have important clinical value and market prospects.

The invention discloses a bone-derived collagen peptide with a joint protection effect and a preparation method thereof, and belongs to the technical field of collagen peptide preparation, the preparation method comprises the following steps: S1, cleaning and crushing fresh fish bones, and performing impurity protein removal treatment on fish bone powder by using a 0.1 M NaOH solution to obtain pretreated bone powder; s2, mixing the pretreated bone meal with 0.5-1.5 mol / L hydrochloric acid according to a solid-to-liquid ratio of 1: 5, and carrying out a decalcification reaction for 2-4 hours to obtain decalcified bone collagen; s3, performing sequential enzymolysis on the decalcified ossein by adopting pepsin and trypsin to obtain enzymatic hydrolysate; and carrying out inactivation, purification, vacuum concentration and freeze drying on the enzymatic hydrolysate to obtain the collagen peptide. According to the collagen peptide and the preparation method, the characteristic amino acid content of the prepared collagen peptide is increased by 40% or above compared with that of a traditional process, it is ensured that the collagen peptide is combined with a cartilage cell receptor in a targeted mode and inhibits secretion of inflammatory factors, and the inhibition rate reaches 58-68%; at a concentration of 500 [mu] g / mL, there is no toxicity to HaCaT cells (IC50gt; and the traditional Chinese medicine composition has no toxic or side effect and no skin irritation.

The application discloses a kind of targeted delivery molecules, particle and its preparation method, application, comprising the following steps: step 1: to the polyethylene glycol molecule A solution containing maleimide group and hydrophobic group is added dropwise with thiol-containing polypeptide targeting factor B solution, stir and react fully;Wherein the amount-of-substance ratio of A and B is 1:1;Step 2: the solution obtained in step 1 is dialyzed, freeze-dried to obtain the required targeted delivery molecules;The targeted delivery molecules prepared by the application have cartilage cell-specific polypeptide targeting factor at one end, and can target cartilage tissue.The hydrophobic group at the other end can be inserted into the hydrophobic lipid bilayer by physical action.The polypeptide targeting factor is assembled on the surface of cell, cell exosome, liposome and other vesicles composed of lipid bilayer, which has the effect of treating osteoarthritis.The above-mentioned cells and vesicles can be targeted to deliver the contents to cartilage, and have good application prospect in the treatment of osteoarthritis.

A tissue-derived implant including: a plurality of decellularized extracellular cartilage particles, wherein each of the plurality of cartilage extracellular particles comprise one or more magnetic particles and mesenchymal stem cells and method of preparation and method use thereof. The tissue-derived implant can be guided to the site of a cartilage defect after administration by use of a magnetic field.

The invention discloses a multivalent triangular DNA origami delivery system as well as a preparation method and application thereof, which can be used as a novel method for treating osteoarthritis, and is particularly suitable for patients with cartilage degeneration and joint inflammation caused by age, obesity, joint reuse, sport injury or genetic factors and the like. Specific siRNAs (small interfering RNAs) are delivered by the use of multivalent triangular DNA origami. According to the scheme, the expression of inflammation and degeneration related genes, such as Stat3, MMP3 and MMP13, in cartilage cells in joints can be specifically inhibited, so that the inflammatory response is relieved, cartilage decomposition is inhibited, and repair and regeneration of cartilage tissues are promoted. The invention provides a method for treating osteoarthritis, which has the advantages of strong targeting property, small side effect and obvious curative effect, is suitable for clinical treatment and basic medical research, and has wide application prospect.

The invention belongs to the technical field of biological medicines, and particularly relates to application of xanthosine monophosphate in preparation of a medicine for treating osteoarthritis. The molecular formula of the xanthosine monophosphate is C10H13N4O9P, and the structure of the xanthosine monophosphate is shown in the specification. Research results show that the flavin monophosphate solution has an inhibition effect on cartilage cell activity; animal model tests show that the flavin monophosphate normal saline solution can remarkably relieve the cartilage injury degree of osteoarthritis mice and relieve osteoarthritis, and a new way is provided for improvement of osteoarthritis conditions. .

The invention belongs to the technical field of biological medicine, and particularly relates to application of a Gli activator in preparation of a product for treating fiberosity. The invention provides an application of a Gli activator in preparation of a product for preventing and / or treating fiber hair disease. The Gli activator comprises an overexpressed Gli adenovirus, an overexpressed Gli adeno-associated virus, a nucleic acid molecule for improving Gli expression and a small molecule compound for activating Gli. The application accidentally finds that the Gli2 adenovirus can remarkably correct the skeleton phenotype of a mouse model of JATD caused by IFT88 deletion, and the activator with the Gli transcription factor as the target spot can promote the expression of a proliferation related gene c-MYC, or n-MYC, or Cyclin D1; the expression of the apoptosis related gene Bcl2 is inhibited; therefore, bone and cartilage cell proliferation is promoted; bone and cartilage cell apoptosis is reduced; and the effect of activating bone and cartilage cells is achieved, so that the fibrinosis is prevented and / or treated, and a potential target and a new treatment scheme are provided for clinical treatment of generalized fibrinosis.

The invention belongs to the field of tissue regeneration materials, and relates to a 3D printing triboelectric material and application thereof in bone and cartilage defect repair. The material can be used as 3D printing ink for manufacturing a triboelectric bracket. The scaffold is prepared by taking biodegradable PGS as a matrix, PEDOT: PSS as a conductive component and sodium chloride as a pore-forming agent through combination of 3D printing and a salt particle pore-forming method, and has a multi-stage porous structure and excellent triboelectric properties. The wire diameter, interlayer spacing and micropore distribution of the stent are accurately regulated and controlled through the 3D printing technology, so that in-situ self-energized electrical stimulation of the stent is realized in an in-vivo dynamic mechanical environment; the scaffold can promote the BMSCs to differentiate into cartilage cells, construct tissue engineering cartilage and accelerate the process of ossification in cartilage, finally realizes efficient repair of bone and cartilage defects, is excellent in biocompatibility and biodegradable, does not need an external power supply device, and has a wide clinical transformation prospect in the field of bone and cartilage defect repair.

The invention relates to regenerated cartilage based on hydrostatic pressure stimulation and a preparation method and application thereof. The method comprises the following steps: inoculating cells on a porous hydrogel scaffold, placing the porous hydrogel scaffold in a culture medium, performing pressure stimulation by adopting a hydrostatic pressure bioreactor, and performing in-vitro culture for a period of time to obtain the regenerated cartilage based on hydrostatic pressure stimulation. Key mechanical sensitive channels TRPV4 and PIEZO1 are up-regulated through hydrostatic pressure stimulation, a Ca < 2 + > dependent TRPV4 / PIEZO1-Ca < 2 + >-SOX9 mechanical transduction pathway is activated, cartilage formation is promoted, and hypertrophy and ossification are inhibited. Through verification, the bioreactor system is combined with the porous hydrogel scaffold, has good biocompatibility, controllable pore structure and mechanical property, and is suitable for cartilage cell culture. After in-vitro pre-culture for 4 weeks, the cartilage-like construct subjected to HP pretreatment shows a long-term stable regeneration effect in an animal model (such as a goat), and especially in an in-situ auricle microenvironment, inflammation and heterotopic ossification can be effectively inhibited, and an efficient in-vitro cartilage engineering strategy is provided for repairing of various cartilage injuries.

The invention relates to the technical field of modern biomedicine, in particular to a method for promoting differentiation of human umbilical cord mesenchymal stem cells based on an EVs composite material, which comprises the following steps: 1, preparing a double-layer composite scaffold; 2, preparation and characterization of EVs: primary cartilage cell culture, enzymatic digestion of cartilage tissue, digestion termination and cell collection, EVs extraction, and EVs characterization identification; 3, loading the EVs; 4, separation, identification and differentiation induction of the hUC-MSCs; and 5, differentiation effect detection. The method has the characteristics that the differentiation efficiency and stability are good, the EVs delivery efficiency is remarkably improved, the biocompatibility and safety are good, the operation is simple and convenient, the cost is controllable, and the application scene is flexible and wide.

The present invention relates to a complex for promoting cartilage differentiation comprising stem cells or a culture thereof and a cartilage cell-free crush, a method for fabricating cartilage by using the complex, cartilage fabricated via the method, a pharmaceutical composition comprising the complex for preventing or treating arthropathy, a method for preventing or treating arthropathy using the composition, a composition for inducing cartilage cell differentiation comprising the prepared cartilage cells, and a method for fabricating cartilage using the prepared cartilage cells. The complex for promoting cartilage differentiation comprising the stem cells or the culture thereof and the cartilage cell-free crush provided in the present invention is not only differentiated into cartilage cells without side effects even in vivo, but also promotes differentiation of inherent stem cells in vivo into cartilage cells via a paracrine effect exhibited in the differentiated cartilage cells to effectively regenerate the damaged cartilage, and thus the complex may be widely used for treatment of arthropathy involving the cartilage damage.

The invention relates to the technical field of bioengineering, in particular to a preparation method and application of a folium cortex eucommiae fermentation extract for promoting cartilage growth. According to the method, a four-stage dynamic regulation and control process of low-temperature plasma synergistic enzymolysis pretreatment, aerobic wall breaking, aerobic and anaerobic transition and anaerobic conversion is adopted; four specific strains with complementary functions, namely bacillus subtilis, aspergillus niger, saccharomyces cerevisiae and enterococcus faecalis, are selected, and precise temperature control and pH regulation are combined, so that efficient release and conversion of active ingredients of the folium cortex eucommiae are realized. The active ingredients of the extract are rich in variety and sufficient in content, cartilage cell proliferation and osteogenic differentiation can be remarkably promoted, and the extract has full-scene cartilage repair adaptability and is good in safety through toxicology verification. The preparation process is stable and repeatable, reaches the pilot test effect, can be adapted to various dosage forms such as oral preparations, injections and the like, and is suitable for preparing cartilage repair related medicines, functional foods and cartilage tissue engineering scaffold composite materials.

Agglomeration and deformation that occur during culture of a three-dimensional cell aggregate are suppressed to create a three-dimensional cell aggregate having a linear shape. A method for producing artificial cartilage tissue, the method comprising: agglomerating cartilage progenitor cells to form cartilage progenitor spheroids; seeding the cartilage progenitor spheroids along grooves in a membrane having the grooves; culturing the cartilage progenitor spheroids in a state in which a culture medium is supplied from the obverse side and the reverse side of the membrane to fuse the spheroids together; and then maturing the spheroids into cartilage-like tissue and / or long bone progenitor cartilage organoids.

The invention discloses a methacrylic acid hyaluronic acid hydrogel microsphere wrapped by a macrophage membrane and application, and relates to the technical field of drug synthesis, the key points of the technical scheme are as follows: the methacrylic acid hyaluronic acid hydrogel microsphere EMMatHMs wrapped by the macrophage membrane can efficiently neutralize key proinflammatory factors, the proinflammatory factors comprise TNF-alpha and IL-1beta, therefore, the joint inflammation is relieved. The compound has good curative effect and biocompatibility in an OA model, and especially has effects in the aspects of regulating macrophage polarization, inhibiting cartilage cell hypertrophy and neutralizing proinflammatory factors. By targeting key links in OA pathogenesis, the EMM-at-HMs not only can significantly reduce inflammation, but also has the potential of delaying the disease progress, and provides a new strategy with breakthrough significance for treatment of OA.

The application provides a developmental growth plate layered scaffold and a preparation method and application thereof, and belongs to the technical field of bone tissue engineering materials. The developmental growth plate layered scaffold provided by the application is divided into three layers, the upper layer is a cartilage cell layer, the middle layer is a blood vessel barrier layer, and the lower layer is a bone formation layer. The blood vessel barrier layer can be used as biological glue to bond the upper layer and the lower layer, so that the composite scaffold structure is stable and is not easy to break; and the upper layer contains bone marrow mesenchymal stem cells (BMSCs), and the middle layer and the lower layer are cell-free. Under the in-vitro chondrogenic induction culture condition, the BMSCs in the upper layer can pass through the middle layer and colonize in the lower layer, and in the process, part of the BMSCs can differentiate into cartilage cells. The middle layer containing thrombospondin 1 (TSP1) has specific intervention effect on cell migration, which is manifested as inhibition of the migration of vascular endothelial cells, but has no effect on the migration of BMSCs.

The invention belongs to the technical field of biological materials, and provides a cartilage regenerative cell sphere-based gel patch and a preparation method thereof. The gel patch is composed of matrix hydrogel and three-dimensional cell balls distributed in the matrix hydrogel in an array mode, the matrix hydrogel is hydrogel of a biocompatible polymer, and the three-dimensional cell balls are obtained by co-culturing bone marrow mesenchymal stem cells and cartilage-like cells according to the quantity ratio of (1-3): 1. The cartilage-like cells are obtained by two-dimensional induced differentiation of bone marrow mesenchymal stem cells. According to the invention, the dependence of the cartilage repair material on natural cartilage cells can be avoided, the cartilage repair material is endowed with stable cartilage generation capability, and meanwhile, the convenience of clinical implantation operation can be increased.

The present invention provides a method for producing an artificial bone-cartilage complex, the method comprising: (i) a step for inducing differentiation of pluripotent stem cells to obtain a precartilage cell mass; (ii) a step for seeding and culturing the precartilage cell mass obtained in the step (i) on the surface of an artificial bone having communication holes; and (iii) a step for recovering an artificial bone-cartilage complex in which a cartilage layer is formed on the surface of the artificial bone. Also provided is an artificial bone-cartilage complex produced by said method.

The invention relates to a long-acting hydrogen release system for assisting cell transplantation as well as a preparation method and application thereof, belongs to the technical field of medical treatment, and provides a long-acting hydrogen release system for assisting cell transplantation in order to provide a substance capable of performing targeted delivery to a cell transplantation part and continuously releasing high-dose H2. The long-acting hydrogen release system comprises a hydrogen release material with a particle size of 5 nm to 10 [mu] m and a hydrogel matrix, and the cells are dispersed in the system; the concentration of the hydrogen release material is 0.01 to 100 [mu] g / mL, and the content of the cells is 10000 to 100000000 cells / mL. The long-acting hydrogen release system is used for maintaining the activity and directional differentiation of transplanted cells in an in-vitro differentiation / balling process and maintaining the activity and characteristics of the transplanted cells in a pathological microenvironment after transplantation. In a malignant pathological microenvironment, hydrogen is continuously supplied, so that the activity of stem cell spheres assembled / differentiated into cartilage cell microspheres and the hyaline cartilage phenotype are effectively maintained, and the activity of transplanted stem cells and the hyaline cartilage differentiation potential are maintained by continuously supplying hydrogen; and continuously supplying hydrogen to maintain the activity and transparency of the transplanted cartilage cells.

The construction method comprises the following steps: preparing a PCL near-field direct-writing inner-layer stent and a PGS / PCL hollow pipeline outer-layer stent, nesting the inner-layer stent and the outer-layer stent outside a silica gel tube to obtain a double-layer stent silica gel tube combination body, fixing the double-layer stent silica gel tube combination body in a bioreactor, soaking the double-layer stent silica gel tube combination body in an alcohol bath, and airing the double-layer stent silica gel tube combination body in an ultra-clean workbench; inoculating pig smooth muscle and cartilage cells on the double-layer bracket for culturing; taking out the double-layer stent silica gel tube combination body from the bioreactor in a sterile state, removing the silica gel tube of the double-layer stent silica gel tube combination body, carrying out decellularization treatment, and retaining an extracellular matrix and bioactive substances; obtaining the artificial blood vessel. The caramel sacrifice template is adopted to prepare a hollow pourable pipeline structure, antibiotics, anticoagulant drugs and the like can be loaded, and infection and embolism are effectively avoided; meanwhile, the acellular extracellular matrix is prepared by adopting an acellular technology, immediate implantation can be realized after engineering preparation is completed, the consumed time is short, and the method is suitable for trauma first aid.

The invention discloses hydrogel microspheres for inhibiting mitochondrial DNA release and improving osteoarthritis and application, and aims at the clinical dilemma that early and middle-stage osteoarthritis lacks specific treatment, the invention discovers that mechanical overload induced mitochondrial membrane potential reduction (from-180mV to-100mV) leads to abnormal opening of VDAC, miMOMP change and mtDNA outleakage, and the hydrogel microspheres can inhibit mitochondrial DNA release and improve osteoarthritis. The method is a key mechanism for steady-state imbalance of cartilage cells. On the basis, a Fe / BiOCl (at) AHAMA-GelMA dual response system is constructed, wherein the Fe / BiOCl nanosheet has positive charges (+ 25-30 mV) in a resting state and can target a mitochondrial inner membrane, and local negative potential (about-50 mV) is generated under mechanical stimulation to release electrons to recover membrane potential; the AHAMA-GelMA hydrogel microspheres are combined with amino groups on the surface of cartilage through aldehyde groups to realize spatial positioning, and a targeted mechanical overload area is recognized by utilizing the specificity of MMPs to gelatin. In-vivo and in-vitro experiments prove that the system can convert overload abnormal stress into repair electric energy, inhibit mtDNA outleakage, remarkably improve the mitochondrial function and relieve cartilage degeneration, and a new disease modification treatment strategy is provided for osteoarthritis.

The invention belongs to the technical field of biomedical materials, and relates to an MMP13-DNA / NVs (at) GA hydrogel sustained-release system and a preparation method and application thereof, and the MMP13-DNA / NVs (at) GA hydrogel sustained-release system is formed by combining aptamer DNA hydrogel with MMP13 response and GA-loaded cell membrane bionic nano-vesicles. Compared with the prior art, the MMP13-DNA / NVs (at) GA hydrogel sustained-release system responds to overexpressed MMP13 in an inflammatory microenvironment, the drug GA for promoting polarization of M1 phenotype macrophages is precisely controlled to drive the M1 phenotype macrophages to be polarized into M2 phenotype, so that TGF-beta is released to promote mesenchymal stem cells to be differentiated into cartilage cells; meanwhile, the accurate delivery efficiency of the vesicles is improved by utilizing cell homology, and a novel strategy with intelligent response and accurate treatment is provided for osteoarthritis treatment.