Tissue engineered meat for consumption and a method for producing tissue engineered meat for consumption

a technology of tissue engineered meat and production method, which is applied in the direction of biochemistry apparatus and processes, food preparation, on/in inorganic carriers, etc., can solve the problems of food contamination, high inefficiency of production method, and current meat production methods that are also harmful to the environmen

Inactive Publication Date: 2005-04-21
VEIN JON
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Meat products are currently produced from whole animals, which is a highly inefficient production method because a significant portion of all agriculturally produced grain is used for animal rather than human consumption.
Current meat production methods are also harmful to the environment.
Another problem with current meat production methods involves food contamination.
The USDA and the FDA, however, rarely regulate the farms where pathogens originate because they lack the regulatory powers over the farms.
Nonetheless, except for E. coli 0156:H7, dangerous bacteria are legally considered “inherent” to raw meat.
The incidence of serious illness and death from these bacteria may increase as more antibiotic-resistant strains develop.
This has caused some scientists to question the continued use of antibiotics as a feed supplement for livestock.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example i

[0024] This example illustrates the isolation of pluri-potent mesenchymal stem cells for use in producing meat products in vitro. Mesenchymal stem cells give rise to muscle cells (myocytes), fat cells (adipocytes), bone cells (osteocytes), and cartilage cells (chrondocytes). Mesenchymal stem cells may be dissected and isolated from embryonic tissues of any non-human animal embryos. In cattle, for example, embryonic mesenchymal tissues that are rich in pluri-potent muscle stem cells are preferably isolated from embryos at day 30 to 40 or earlier. Once dissected, the embryonic tissues may be minced into small pieces about one millimeter by one millimeter in size in phosphate buffered saline (“PBS”) pH 7.45. Five to ten pieces of the minced tissue may be incubated in 300 μl of 0.25% trypsin and 0.1% EDTA in PBS for thirty minutes at 37° C. with gentle agitation. Afterwards, the tissues may be allowed to settle on the bottom of the tube by gravity or gentle centrifugation. The supernata...

example ii

[0026] After mesenchymal stem cells have been isolated, they may be enriched for myoblasts or muscle stem cells in culture. Initially, the cells may be differentially plated on different petri dishes after dissociation and washing as described in Example I. Using a 60 mm petri dish, the cells may first be incubated in complete medium for two to four hours. During this time, epithelial cells will tend to attach quickly to the petri dish while the myoblasts remain in the supernatant. The supernatant may then be collected and the myoblasts may be plated on a different petri dish coated with natural or synthetic biomaterials such as those mentioned in Example I. Myoblasts may be enriched by supplementing the growth media with growth factors such as skeletal muscle growth factor, prostaglandin F2α (“PGF2α”), and insulin-like growth factor I (“IGF-1”).

[0027] Further, myoblasts may be differentiated into specific myoctes or muscle cells by culturing the myoblasts in complete medium or in ...

example iii

[0028] Alternatively, myoblasts may be enriched from toti-potent embryonic stem cells. Toti-potent cells may be derived from in vitro fertilized eggs of an animal using in vitro fertilization techniques, from stem cells present in umbilical cords or placenta, or from Embryonic Stem (ES) cells isolated from cells at the blastocyst stage. ES cells, for example, may be collected, gently dissociated by trypsin, and cultured in vitro with recombinant leukemia inhibitory factor (Chemicon, San Diego, Calif.) and feeder cells such as growth arrested embryonic fibroblasts cells. These toti-potent cells may be treated with growth factors such as PGF2α or IGF-1 to induce the cells to differentiate into myoblasts.

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Abstract

A non-human tissue engineered meat product and a method for producing such meat product are disclosed. The meat product comprises muscle cells that are grown ex vivo and is used for food consumption. The muscle cells may be grown and attached to a support structure and may be derived from any non-human cells. The meat product may also comprise other cells such as fat cells or cartilage cells, or both, that are grown ex vivo together with the muscle cells.

Description

RELATED APPLICATIONS [0001] The present application is a continuation of U.S. application Ser. No. 09 / 991,544, filed Nov. 16, 2001, which claims priority to U.S. Provisional Patent Application No. 60 / 60 / 249,993, filed Nov. 17, 2000, both of which are incorporated herein in their entirety by reference.FIELD OF THE INVENTION [0002] The field of the present invention relates to producing and harvesting meat products for consumption. In particular, it relates to tissue engineered meat for consumption. BACKGROUND OF THE INVENTION [0003] Meat products such as beef, pork, lamb, poultry, or fish are desirable products for food consumption. Meat products are currently produced from whole animals, which is a highly inefficient production method because a significant portion of all agriculturally produced grain is used for animal rather than human consumption. In the United States, for example, livestock feed accounts for approximately 70% of all the wheat, corn, and other grain produced. In a...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A01K67/027A23L13/00A23L13/50A23L17/00A61K47/00C12N5/00C12N5/02C12N5/06C12N11/08C12N11/14
CPCA23L1/31A23L1/315Y10S426/802C12N5/0062A23L1/325A23L13/00A23L13/50A23L17/00
Inventor VEIN, JON
Owner VEIN JON
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