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Composition and method for inducing mesenchymal stem cells to be differentiated to cartilage cells

A technology of mesenchymal stem cells and chondrocytes, applied in the field of biomedical engineering, can solve problems such as the need to improve the induction orientation

Inactive Publication Date: 2016-06-22
安沂华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it was found that in this method, the orientation of induction still needs to be improved, for example, a certain amount of other cells such as adipocytes and stromal cells

Method used

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  • Composition and method for inducing mesenchymal stem cells to be differentiated to cartilage cells
  • Composition and method for inducing mesenchymal stem cells to be differentiated to cartilage cells
  • Composition and method for inducing mesenchymal stem cells to be differentiated to cartilage cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] This example is operated according to Example 1 of CN102899287A, the only difference is that the induction differentiation medium contains an induction composition of the following composition: 50 μg / ml L-ascorbic acid-2-phosphate, 5 ng / ml TGFβ3, 100 nM dexamethasone, 25 μg / ml vitamin C, 20 μg / ml proline, 2.5 μg / ml indomethacin, 1×ITS+1premix, 1 μg / ml chlormadinone ester represented by formula (I). Such as figure 1 As shown, the fuchsia long spindle cells demonstrate the successful acquisition of chondrocytes by this method. Compared with the differentiation results of Example 1 of CN102899287A, for example, it can be seen from the comparison of the differentiation photos that the differentiation effect and differentiation orientation of Example 1 of the present invention are obviously better, for example, the morphology is better and the phenotype is stable.

Embodiment 2

[0052] This example differs from Example 1 only in that the inducing composition also contains 100 μM Saussurea tianshanensis extract. The snow lotus extract is prepared according to the extraction method described above.

[0053] refer to image 3 , compared with Example 1, the differentiation orientation in Example 2 is better, and other cells other than chondrocytes are hardly seen.

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Abstract

The invention provides a composition and a method for inducing mesenchymal stem cells to be differentiated to cartilage cells. The composition comprises 20-80 uM of L-ascorbic acid-2-phosphate, 2-10 ng / ml of TGF beta 3, 50-150 nM of dexamethasone, 10-50 ug / ml of vitamin C, 10-50 ug / ml of proline, 0.5-5 ug / ml of indometacin, 1xITS+1premix and 0.5-10 ug / ml of chlormadinone ester. The combination when being used as an inducer of a culture medium can improve differentiation activity and differentiation directionality.

Description

technical field [0001] The invention relates to the field of biomedical engineering, and more specifically relates to a composition and an induction method for mesenchymal stem cells induced to cartilage. Background technique [0002] Mesenchymal stem cells are a type of adult stem cells with high self-renewal and multi-lineage differentiation potential. They have strong self-replication ability and multi-lineage differentiation potential. Under certain induction conditions, they can differentiate into osteoblasts, chondrocytes, Adipocytes, etc., have been widely used in cartilage tissue engineering and gene therapy as seed cells and cell carriers in recent years. [0003] At present, the method of using mesenchymal stem cells to differentiate into chondrocytes is generally to culture mesenchymal stem cells to subcultured mesenchymal stem cells, and then conduct induction culture in a medium containing factors that are directed to induce chondrocytes. Mesenchymal stem cells...

Claims

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Application Information

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IPC IPC(8): C12N5/0775C12N5/077
CPCC12N5/0655C12N2500/32C12N2500/38C12N2500/76C12N2501/15C12N2501/39C12N2501/392C12N2501/999C12N2506/1353C12N2506/1369
Inventor 安沂华董健伸
Owner 安沂华
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