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A technology of preservation solution and cartilage, applied in the field of biomedical engineering, can solve problems such as joint degeneration or defect, decreased biological activity, etc., and achieve the effect of solving degeneration
Inactive Publication Date: 2012-01-11
FOURTH MILITARY MEDICAL UNIVERSITY
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[0008] The present invention can overcome the problems of biomechanical influence, ultrastructural changes of chondrocytes and vascular endothelial cells, and decline in biological activity in the preservation of cartilage joints at the present stage, and is applicable to cartilage lesions that may occur in people of all ages and will be more frequent than before post-traumatic arthritis resulting from cartilage lesions that progress to joint degeneration or loss
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Embodiment 1
[0010] Example 1: Each 1000ml cartilage preservation solution contains 20mmol of potassium dihydrogen phosphate, 80mmol of histidine, 75mmol of lacturonic acid, 60mmol of sucrose, 1.0mmol of allopurinol, low molecular weight dextran-40: 50g, Nacl 45mmol, KCl 35mmol, Magnesium sulfate 5mmol, penicillin 75U, reduced glutathione 2.5mmol, adenosine 6mmol, trehalose 4g, the balance is deionized water, and the pH value of the cartilage preservation solution is 7.33-7.53. Aliquot, seal, and store at 0-4°C.
Embodiment 2
[0011] Example 2: Each 1000ml cartilage preservation solution contains 30mmol of potassium dihydrogen phosphate, 80mmol of histidine, 95mmol of lacturonic acid, 70mmol of sucrose, 1.2mmol of allopurinol, low molecular weight dextran-40: 55g, Nacl 55mmol, KCl 25mmol, Magnesium sulfate 7mmol, penicillin 90U, reduced glutathione 3mmol, adenosine 5mmol, trehalose 5g, the balance is deionized water, the cartilage preservation solution has a pH value of 7.33 to 7.53. Aliquot, seal, and store at 0-4°C.
Embodiment 3
[0012] Example 3: Each 1000ml cartilage preservation solution contains 25mmol of potassium dihydrogen phosphate, 80mmol of histidine, 85mmol of lacturonic acid, 50mmol of sucrose, 0.8mmol of allopurinol, low molecular weight dextran-40: 45g, Nacl 48mmol, KCl 30mmol, Magnesium sulfate 4 mmol, penicillin 80 U, reduced glutathione 4 mmol, adenosine 7 mmol, trehalose 2 g, the balance is deionized water, and the cartilage preservation solution has a pH value of 7.33 to 7.53. Aliquot, seal, and store at 0-4°C.
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Abstract
The invention relates to a cartilage preservation solution. Each 1000ml of the preservation solution comprises 20-30mmol of potassium dihydrogen phosphate, 80mmol of histidine or 15mmol of a histidine salt, 75-95mmol of lactobionic acid, 50-70mmol of sucrose, 0.8-1.2mmol of allopurinol, 45-55g of low molecular dextran-40, 45-55mmol of NaCl, 25-35mmol of KCl, 3-7mmol magnesium sulfate, 70-90U of penicillin, 2-4mmol of reduced glutathione, 3-7mmol of adenosine, 2-5g of trehalose, and the balance deionized water, and the pH value of the cartilage preservation solution is 7.33-7.53. The cartilagepreservation solution is packaged, sealed and preserved at 0-4DEG C. By adopting the preservation solution to preserve cartilage tissues, the death speed of cartilage cells is slowed, the activity ofcartilage fibers is maintained, the performance of the cartilage tissues is kept, and the antibiotic property is increased, so the preservation solution has a good preservation effect and a long preservation time on the cartilage tissues, and is in favor of clinic cartilage tissue transplant operations.
Description
Technical field [0001] The invention belongs to the field of biomedical engineering, and specifically relates to a cartilage preservation solution that can solve the problem of degeneration of the same type of articular cartilage after a transplantation operation after the preservation is performed, and a preparation method thereof. Background technique [0002] As a composite tissue, the biological activity of cartilage joints after preservation is affected by many factors. Joint defects caused by trauma, tumors or other causes are a difficult problem in medical treatment. Many scholars are exploring a simple and safe way to preserve cartilage joints for a long time, so that the biomechanical effects of cartilage joints and the ultrastructural changes of chondrocytes and vascular endothelial cells after preservation can achieve better conditions and biological activity. [0003] As more and more young people participate in competitive sports and leisure activities, men and women o...
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