76 results about "Cartilage tissues" patented technology

The invention relates to application of a miR-155-5p inhibitor delivered by an exosome of a targeted synovitis macrophage to treatment of osteoarthritis, and belongs to the technical field of biological medicines. According to the scheme, the exosome drug delivery system for targeting the synovial inflammatory macrophages in the osteoarthritis, which contains the miR-155-5p inhibitor, is prepared through a genetic engineering technology, so that the exosome derived from stem cells is targeted to inflammatory synovial tissues, and damaged cartilage tissues are repaired and inflammation is resisted based on the stem cell exosome; compared with the prior art, the application has the advantages that the combined action of inhibiting the osteoarthritis injury and inhibiting the miR-155-5p so as to resist synovitis is realized, the osteoarthritis injury repair can be better applicable, a new scheme for treating the osteoarthritis is provided, a support is provided for clinical transformation, and the application value is extremely high.

Provided is a method for simply and inexpensively producing a high-purity proteoglycan from a crushed product of cartilage tissue, a squeezed product of the crushed product, or an extract of the crushed product or the squeezed product.　In the present invention, a crushed product of cartilage tissue, a squeezed product of the crushed product, or an extract of the crushed product or the squeezed product is immersed in or added to an aqueous solution containing a salt, and an alcohol is added to the obtained liquid composition in such an amount as to have a final concentration of 10-60 vol%, whereby a proteoglycan is precipitated. Alternatively, a crushed product of cartilage tissue, a squeezed product of the crushed product, or an extract of the crushed product or the squeezed product is immersed in or added to an aqueous solution containing a salt and an alcohol in such an amount as to have a final concentration of 10-60 vol%, whereby a proteoglycan is precipitated.

The invention belongs to the technical field of biological medicines, and discloses a B cell and cartilage cell senescence targeting osteoarthritis treatment medicine. The invention provides an application of an anti-MIF antibody or an antigen binding fragment thereof as a B cell targeting immunomodulator in osteoarthritis treatment drugs, the anti-MIF antibody or the antigen binding fragment thereof regulates and controls an MIF / HMGB1 / MMP13 signal axis through a neutralization effect with MIF, so that the MIF and B cells are significantly co-localized, expression of HMGB1 in the B cells is promoted, and the osteoarthritis treatment effect is improved. The senescence of B cells is reduced and the B cells are promoted to regulate or repair phenotype transformation so as to destroy chronic inflammatory circulation and diffusion of pathogenic plasma cells, and the expression of MMP13 in joint tissues is reduced so as to maintain the steady state of cartilage tissues; the preparation method has an excellent application prospect in preparation of an OA intra-articular injection drug which realizes rapid anti-inflammatory and long-term cartilage protection effects and can realize a lasting curative effect through low-frequency drug delivery.

The invention relates to polyglutamic acid bio-ink with antibacterial and antioxidant functions as well as a preparation method and application of the polyglutamic acid bio-ink. The method comprises the following steps: firstly, degrading high-molecular-weight gamma-polyglutamic acid at a specific high temperature to obtain degraded gamma-polyglutamic acid (d gamma-PGA) which is low in viscosity and suitable for printing; then, the gamma-polyglutamic acid (d gamma-PGAGMA) is reacted with a methacrylic acid acylation reagent, and the photo-crosslinkable gamma-polyglutamic acid (d gamma-PGAGMA) degraded by methylacryloylation is prepared; and finally, mixing the dgamma-PGAGMA, a photoinitiator and the living cells to obtain the biological ink. The hydrogel is suitable for 3D biological printing and can be formed at will, and the hydrogel formed through printing has the effects of inhibiting bacteria survival and resisting oxidation and can promote growth of cartilage cells and regeneration of cartilage tissue.

The present invention relates to a method for producing cartilage tissue by selecting and choosing from chondrocytes or spheroids, and use thereof as pharmaceutical composition, medicinal product or transplant. In particular, the invention relates to markers for identifying suitable chondrocytes for producing cartilage tissue, in particular for the purpose of transplantation.

Large volume cellular material may be preserved by combining the cellular material with a cryoprotectant formulation / medium / solution containing at least one mNP and then subjecting the cellular material to a vitrification preservation protocol including nanowarming. This preservation method is particularly effective for cartilage tissues.

The present invention discloses an integrated bone and cartilage scaffold with a directional pore structure and a preparation method thereof, which belongs to the fields of tissue engineering, biomedicine and biomanufacturing technology. The method simulates the natural material and structure of bone cartilage, uses the principle of directional crystallization to prepare a bone and cartilage scaffold with a directional structure, and uses a low-temperature deposition manufacturing process based on rapid prototyping technology to prepare a horizontally oriented cartilage layer scaffold and a vertically oriented subchondral bone layer scaffold. Between the cartilage layer and the subchondral bone layer, methacrylated gelatin capable of UV cross-linking is combined with other materials with chemical grafting and bonding effects as a transition layer, thereby preparing a tissue engineering bone and cartilage integrated composite scaffold. The present invention is aimed at the treatment of articular bone and cartilage defects, and uses a bioactive material to manufacture a bone and cartilage composite scaffold with a directional pore structure. It can promote the coordinated repair of bone and cartilage tissue according to the characteristics of different functional areas of bone and cartilage, thereby improving the repair effect of bone and cartilage defects.

The invention relates to a functionalized cartilage tissue repair material as well as a preparation method and application thereof, and belongs to the technical field of tissue engineering. The invention aims to solve the problems that the existing decalcified bone matrix is insufficient in cartilage induction in cartilage repair, and is easy to cause blood vessel ingrowth and fibrosis repair. The preparation method comprises the following steps: inoculating cartilage cells on a three-dimensional porous frame structure carrier, carrying out in-vitro dynamic pre-culture to enable the cartilage cells to secrete an extracellular matrix, and carrying out decellularization treatment to obtain the functionalized cartilage tissue repair material retaining the cartilage specific matrix. The material has a suitable pore structure and abundant II-type collagen deposition, can effectively promote chondrocyte differentiation and hyaline cartilage regeneration, inhibits blood vessel ingrowth, maintains a cartilage vascular-free microenvironment, significantly improves the structure and function reconstruction effect of cartilage defect repair, and has good biocompatibility and clinical transformation potential.

According to the application of the triple-network hydrogel to preparation of the osteochondral defect repair material, a PRP-coated OSA-GEL three-network composite hydrogel system is provided based on PRP, OSA and gelatin, in the system, a dynamic covalent network of OSA and gelatin, a physical network of fibrin and an egg box model formed between Ca < 2 + > and OSA form an IPN network with enhanced mechanical properties, and the PRP-coated OSA-GEL three-network composite hydrogel system has the advantages that the mechanical properties of the PRP-coated OSA-GEL three-network composite hydrogel system are enhanced; a dynamic cross-linking system of the PRP-coated OSA-GEL hydrogel not only can support cell infiltration and tissue regeneration, but also brings self-repairing capability to the hydrogel, so that a hydrogel network can be quickly recovered after the hydrogel is broken, the vulnerable biological activity of PRP is protected, new cartilage of the PRP-coated OSA-GEL is complete, regenerated cartilage tissue in a defect area is almost completely fused with surrounding normal cartilage, and the defect area of the PRP-coated OSA-GEL is not damaged. It is indicated that the PRP remarkably enhances the cartilage regeneration effect through PRP-coated OSA-GEL, and the PRP has effectiveness in the aspect of promoting and enhancing osteochondral regeneration.

Embodiments relate to an imidazolidine derivative or a pharmaceutically acceptable salt thereof and its use for preventing, ameliorating, or treating arthritis. The compound of one embodiment can be effectively utilized for preventing, ameliorating, or treating arthritis by inhibiting degeneration or damage to cartilage tissue through inhibition of the activity of ADAMTS4 or ADAMTS5.

The present invention provides an integrated design and manufacturing technology for a bone cartilage biomimetic gradient scaffold. The invention includes a functionally graded integrated bone cartilage biomimetic gradient scaffold, a transition structure for achieving graded transition between hard bone and cartilage tissue, and a biomimetic cartilage formation and post-processing method. The integrated bone cartilage biomimetic gradient scaffold is a porous interconnected ceramic structure that can be manufactured through integrated additive manufacturing. The transition structure is a special structure for firmly connecting hard bone and cartilage. The biomimetic cartilage formation and post-processing method involves performing special treatment on the 3D-printed bone scaffold to form biomimetic cartilage, thereby improving the strength of the cartilage layer and preventing cartilage damage caused by joint friction. The bone cartilage biomimetic scaffold structure proposed by the present invention has effective functional grading to mimic natural bone cartilage tissue, which is beneficial for preventing stress shielding and can be manufactured in one piece using 3D additive manufacturing technology.

The application discloses a multi-component synovitis treatment composition based on repairing cartilage tissue and application thereof, and relates to the technical field of biological medicine, and the composition is composed of glucosamine hydrochloride, chondroitin sulfate, dimethyl sulfone and graded molecular weight sodium hyaluronate; the application precisely compounding the graded molecular weight sodium hyaluronate with the glucosamine hydrochloride, the chondroitin sulfate and the dimethyl sulfone according to weight parts, constructs a gradient slow-release and target delivery system of synovitis lesions, specifically inhibits the activity of hyaluronidase in the inflammatory joint cavity by using the dimethyl sulfone, greatly reduces the degradation of the sodium hyaluronate, prolongs the residence time of the active ingredients at the lesion site, realizes immediate analgesia and relieves joint stiffness by quickly forming a lubricating protective film by the high molecular weight sodium hyaluronate, the medium and low molecular weight sodium hyaluronate penetrates the synovial membrane barrier and carries the cartilage repair components to enrich in the cartilage defect area, and the synergistic effect of the components is realized.

The application discloses a kind of bionic bone / cartilage double-layer composite scaffold material and its preparation method and application.The bionic bone / cartilage double-layer composite scaffold material of the application is composed of upper cartilage repair hydrogel layer and lower bone repair scaffold layer.The main feature of the application is that by radial freezing, the chitosan-chitin whisker liquid crystal composite hydrogel micron radial channel with diameter gradient change is constructed in the lower scaffold, which can promote the transport of nutrients, cell recruitment, migration and adhesion;At the same time, the drug-loaded liquid crystal hydrogel constructed in the longitudinal large channel of the scaffold and the upper cartilage repair layer can realize the sustained release of active drugs, so as to further regulate stem cell osteogenesis / cartilage differentiation and promote the generation of blood vessels, and is expected to have good application prospect as biomedical materials such as bone / cartilage tissue repair materials.

The present invention relates to a polymer composition for making a bone anchoring component of an orthopedic implant for repairing damaged cartilage tissue, the composition comprising a biostable thermoplastic polyurethane and 15-70% by mass of micron-sized inorganic particles, the particles comprising a biocompatible transition metal compound. Studies have found that the relatively rigid thermoplastic polyurethane composition comprising the inorganic particles allows the production of an anchoring component that can be inserted or press-fit into a pre-drilled bone hole to form a firm and lasting connection with bone tissue, the connection being visualized with, for example, X-rays or MRI methods. The polymer composition shows good performance and can provide freedom in designing and manufacturing implants using common techniques such as injection molding. In other aspects, the present invention relates to a method for manufacturing the polymer composition, and using the polymer composition to manufacture an orthopedic implant comprising a bone anchoring component, such as forming an implant using a multi-component injection molding process, the process comprising molding the bone anchoring component with the polymer composition.

The present disclosure relates to a method of treating arthritis by targeting Tankyrase. The methods according to the present disclosure can be advantageously used for regeneration of cartilage tissue and for treating osteoarthritis by maximizing the matrix synthesis in cartilage by inhibition of Tankyrase and regulation of other proteins related therewith.

The present invention discloses a method for extracting chondroitin sulfate by enzymatic-deep eutectic solvent coupling, comprising the steps of extracting chondroitin sulfate from animal cartilage tissue raw material using a protease-assisted deep eutectic solvent, wherein the protease comprises one or both of a serine protease and a metalloprotease, and the raw materials for preparing the deep eutectic solvent include betaine and glucose. The present invention further claims protection for a chondroitin sulfate product prepared based on the protease-assisted deep eutectic solvent method for extracting chondroitin sulfate. The present invention utilizes a protease-assisted deep eutectic solvent to extract chondroitin sulfate, greatly improving both extraction efficiency and extraction purity, and the extraction method is environmentally friendly.

The application discloses a kind of targeted delivery molecules, particle and its preparation method, application, comprising the following steps: step 1: to the polyethylene glycol molecule A solution containing maleimide group and hydrophobic group is added dropwise with thiol-containing polypeptide targeting factor B solution, stir and react fully;Wherein the amount-of-substance ratio of A and B is 1:1;Step 2: the solution obtained in step 1 is dialyzed, freeze-dried to obtain the required targeted delivery molecules;The targeted delivery molecules prepared by the application have cartilage cell-specific polypeptide targeting factor at one end, and can target cartilage tissue.The hydrophobic group at the other end can be inserted into the hydrophobic lipid bilayer by physical action.The polypeptide targeting factor is assembled on the surface of cell, cell exosome, liposome and other vesicles composed of lipid bilayer, which has the effect of treating osteoarthritis.The above-mentioned cells and vesicles can be targeted to deliver the contents to cartilage, and have good application prospect in the treatment of osteoarthritis.

The invention concerns a polymer composition for making a bone anchoring part of an orthopedic implant for use in repair of damaged cartilage tissue, comprising a biostable thermoplastic polyurethane and 15-70 mass % of micron-sized inorganic particles comprising a biocompatible transition metal compound. It was found that the relatively rigid TPU composition comprising said inorganic particles allows making an anchoring part that can be inserted or press-fitted into a pre-drilled bone hole to form a firm and durable connection to bone tissue, and which may be visualized with for example X-ray or MRI methods. The polymer composition shows favorable properties and enables freedom in designing and making the implant with common techniques like injection molding. In other aspects, the invention relates to methods of making said polymer composition, and to using the polymer composition in making an orthopedic implant comprising a bone anchoring part, like in forming the implant with a multi-component injection molding process comprising a step of molding the bone anchoring part from the polymer composition.

The utility model relates to the technical field of surgical instruments, and discloses a combined cannula for an intervertebral foramen endoscope operation, which comprises a protective tube seat and a protective cannula connected with the protective tube seat, and the tube seat is disc-shaped and is rotatably connected with a working tube seat. A circular groove is formed in the bottom of the working pipe seat, a working sleeve is arranged in the circular groove and communicated with the working pipe seat, and the working sleeve is inserted into the protective sleeve. By means of the design that the working tube base is rotationally connected with the working sleeve, the round groove in the bottom of the working tube base wraps and protects the tube base, the operation sleeve can rotate flexibly in the using process, and the convenience and efficiency of an operation are improved. The working cannula can be flexibly inserted into the protective cannula, combined use is convenient, stability of the operation cannula is guaranteed, possible damage to cartilage tissue and vascular nerves in the operation process is effectively avoided, safety and accuracy of the operation are guaranteed, and the operation cannula has the advantages of being easy and convenient to operate, safe, reliable, accurate and efficient, and has wide application prospects. And the requirements of modern operations on high efficiency, safety and accuracy can be met, and wide market application prospects are achieved.

The invention belongs to the technical field of biological materials and tissue engineering, and relates to functionalized silk fibroin-based bio-ink, bionic cartilage hydrogel and a preparation method thereof. The ink contains an initiator, SFMA, GelMA, PEGDA and OBC, and the ratio of all the components is strictly controlled; after the ink is uniformly mixed with stem cells, a curing cross-linking reaction is initiated, and the multi-bionic cartilage hydrogel is prepared. The hydrogel comprises hydrogel with a multi-network structure as well as OBC and stem cells which are simultaneously dispersed in the hydrogel with the multi-network structure, and has a compression modulus matched with a real cartilage tissue and a porous structure. The functionalized silk fibroin-based bio-ink and the multiple bionic cartilage hydrogel provided by the invention solve the problems of insufficient donors, immunological rejection, single bionic dimension, unmatched mechanical properties and the like in the prior art, and can realize high-precision printing and efficiently promote cartilage regeneration.

The utility model provides an enzymolysis device of chondroitin sulfate, which relates to the technical field of health-care food production, and comprises an operation table, a filter box and a collection box are fixed at the bottom of the operation table, one side of the filter box is connected with one side of the collection box, a filter assembly is arranged in the filter box, and the other side of the filter box is connected with the collection box. The filtering assembly reciprocates between the filtering box and the collecting box, a cleaning assembly is arranged in the collecting box, and when the filtering assembly reciprocates and passes through the cleaning assembly, the surface of the filtering assembly is cleaned by the cleaning assembly. The problem that waste liquid is difficult to permeate into the filter box from the surface of the filter assembly due to the fact that cartilage tissue, protein precipitates, other insoluble substances and the like which are not fully degraded adhere to the filter assembly in the filtering process and block meshes of the filter assembly is solved, and the production efficiency of health care products is further improved.

This invention provides a hybrid exosome nanomotor driven by a pathological microenvironment, its preparation method, and its applications, belonging to the field of biomedical technology. The hybrid exosome nanomotor provided by this invention can utilize the unique oxidative microenvironment of the osteoarthritis lesion area to achieve self-driven movement, thereby actively and efficiently penetrating the dense extracellular matrix of cartilage tissue, significantly enhancing its enrichment and retention capacity in the lesion area, and promoting the specific uptake of therapeutic molecules by chondrocytes. This system not only achieves intelligent response and utilization to the disease microenvironment but also simultaneously alleviates local oxidative stress, ultimately achieving efficient, precise, and sustained therapeutic effects. This invention provides a novel, proactively targeted delivery strategy and treatment platform with promising clinical translation prospects for diseases such as osteoarthritis involving dense tissues or inflammatory microenvironments.

The invention provides a construction method of a growth disorder mouse model based on a Cre / LoxP cartilage tissue conditional knockout Ankrd11 gene, and belongs to the technical field of biology. The construction method disclosed by the invention comprises the following steps: enabling an Ankrd11fl / fl mouse to be mated with a Col2a1-CreERT2 tool mouse, so as to obtain Ankrd11fl / wt; the mouse is a Col2a1-CreERT2 mouse; the Ankrd < 11 > fl / wt; the Col2a1-CreERT2 mouse and the Ankrd11fl / fl mouse are subjected to mating, and the Ankrd11fl / fl mouse is obtained, and the Col2a1-CreERT2 mouse and the Ankrd11fl / fl mouse are subjected to mating; a Col2a1-CreERT2 mouse is subjected to tamoxifen induction treatment, and the growth disorder mouse model is obtained. The construction method provides an animal experiment model for further researching the pathogenic mechanism of the KBG syndrome growth disorder phenotype.

The invention discloses a nano polymer for specific targeted degradation of COX-2, a preparation method and application, and belongs to the technical field of biological medicines. The nano polymer comprises polyethylene glycol methacrylate with ROS responsiveness, a functional monomer for targeted degradation of COX-2, and a ligand structure monomer for recruiting E3 ligase. The invention proves that compared with a single COX-2 inhibitor, the prepared PKP-NP nano polymer has more excellent treatment performance. The problem of hydrophobicity of a traditional PROTAC medicine is solved, COX-2 protein is specifically and continuously degraded, cartilage cell apoptosis is inhibited, and cartilage tissue damage is repaired; the composition can accurately gather in an articular cavity by virtue of inflammation microenvironment response, prolong in-vivo retention time, relieve joint inflammation and repair cartilage injury, and has no obvious side effect. An efficient, safe and lasting scheme is provided for osteoarthritis treatment, and a new idea for clinical treatment is developed.

The invention relates to a method for extracting chondroitin sulfate sodium by a one-step method through superposition of multiple complex enzymes and specific trypsin, which comprises the following steps: S1, cleaning animal cartilage tissue, freeze-drying, and crushing into powder; s2, adding the powder into a buffer solution of a compound enzyme formed by combining specific trypsin 201, recombinant protease 017, lipase, cellulase, pancreatin and neutral protease, and carrying out enzymolysis reaction at 40 DEG C for 4 hours; s3, after the enzymolysis reaction is finished, performing centrifugal separation, ethanol precipitation, filtration, washing and drying to obtain a sodium chondroitin sulfate product. According to the extraction method disclosed by the invention, through the synergistic effect of various complex enzymes, cartilage tissues can be degraded more sufficiently, the yield of sodium chondroitin sulfate is improved, the loss of an intermediate extraction step is reduced by a one-pot method, and the extraction rate of a final product reaches 17% and is 10% higher than that of a traditional extraction rate.

The invention relates to a tissue scaffold developed for use in the treatment of cartilage tissue loss and a method for its production.

The application relates to the field of biological medicines, in particular to a cartilage tissue repair hydrogel and a preparation method thereof. The preparation components of the cartilage tissue repair hydrogel are doped with 10-15 g of mulberry silk, 0.1-0.5 g of glycidyl methacrylate, 0-1 g of lithium magnesium silicate, 0-1 g of tannic acid and 0.1 g of a photo initiator per 100 ml of the hydrogel. The preparation method of the cartilage tissue repair hydrogel is as follows: degumming and dissolving treatment are conducted on the mulberry silk to obtain a silk fibroin solution, glycidyl methacrylate is added into the silk fibroin solution and stirring is conducted to obtain a methacrylated silk fibroin solution, lithium magnesium silicate solution and tannic acid solution are added into the methacrylated silk fibroin solution to obtain a silk fibroin mixed solution, and then the photo initiator is added into the silk fibroin mixed solution to prepare the cartilage tissue repair hydrogel. The tissue repair hydrogel has a hydrophilic three-dimensional network structure, can provide mechanical support and biological induction activity for a bone cartilage defect site, and is suitable for bone injury repair.

The utility model provides a conical grinding drill, which relates to the technical field of medical instruments and comprises a grinding drill bit, a connecting rod and a joint operation power device. The grinding drill bit is conical or truncated cone-shaped, and the small-diameter end of the end face of the grinding drill bit is connected with one end of the connecting rod and used for grinding cartilage tissue and bone tissue of the abraded joint through the large-diameter end of the end face of the grinding part; the other end of the connecting rod is connected with a joint operation power device which is used for driving the connecting rod to rotate, and then the grinding drill bit is driven to rotate through the connecting rod. According to the conical grinding drill, the conical or circular-truncated-cone-shaped grinding drill bit is arranged at one end of the connecting rod, cartilage tissue and bone tissue of a worn joint are ground through the end, with the large diameter, of the end face of the grinding drill bit, and the section diameter of the grinding drill bit at an operative incision is small; and the rotating space required to be reserved for the grinding drill bit is small, so that the grinding drill bit can grind in a smaller surgical incision.

The application provides a kind of cartilage polypeptide composition and its preparation method, the cartilage polypeptide 30-45%, auxiliary agent 10-25%, growth agent 3-8%, slow-release agent 2-9%, the balance is water, the application will cartilage polypeptide, auxiliary agent, growth agent and slow-release agent several ingredients scientific compatibility, preparation has the composition of protecting cartilage tissue, anti-inflammatory pain, effectively increase bone density effect, improve synovial tissue, there is no rebound ineffective condition after drug withdrawal.

The utility model discloses an autologous chondrocyte culture device for cartilage tissue repair, which comprises a culture device body and a culture shelf body, the culture device body comprises a box body, and a constant temperature control system is arranged in the box body; the culture shelf body comprises a plurality of placing plates, the placing plates are detachably mounted in the box body, a plurality of vent holes are formed in the placing plates, a plurality of connecting blocks are rotatably connected to the inner sides of the left side wall and the right side wall of the box body, sliding rails are arranged in the connecting blocks, and the sliding rails are arranged in the sliding rails. And sliding blocks are fixedly connected to the left end and the right end of the placement plate. The placing plate is rotatably mounted in the culture device, and when the culture dish needs to shake, the culture dish can be driven to shake through rotation of the placing plate, so that the shake of the culture dish does not need to be taken out from the culture device, and the culture dish is in a constant-temperature environment while being conveniently shaken; the culture of cartilage cells is facilitated.