33 results about "Cartilage tissues" patented technology

The invention relates to polyglutamic acid bio-ink with antibacterial and antioxidant functions as well as a preparation method and application of the polyglutamic acid bio-ink. The method comprises the following steps: firstly, degrading high-molecular-weight gamma-polyglutamic acid at a specific high temperature to obtain degraded gamma-polyglutamic acid (d gamma-PGA) which is low in viscosity and suitable for printing; then, the gamma-polyglutamic acid (d gamma-PGAGMA) is reacted with a methacrylic acid acylation reagent, and the photo-crosslinkable gamma-polyglutamic acid (d gamma-PGAGMA) degraded by methylacryloylation is prepared; and finally, mixing the dgamma-PGAGMA, a photoinitiator and the living cells to obtain the biological ink. The hydrogel is suitable for 3D biological printing and can be formed at will, and the hydrogel formed through printing has the effects of inhibiting bacteria survival and resisting oxidation and can promote growth of cartilage cells and regeneration of cartilage tissue.

The present invention relates to a method for producing cartilage tissue by selecting and choosing from chondrocytes or spheroids, and use thereof as pharmaceutical composition, medicinal product or transplant. In particular, the invention relates to markers for identifying suitable chondrocytes for producing cartilage tissue, in particular for the purpose of transplantation.

The invention relates to a functionalized cartilage tissue repair material as well as a preparation method and application thereof, and belongs to the technical field of tissue engineering. The invention aims to solve the problems that the existing decalcified bone matrix is insufficient in cartilage induction in cartilage repair, and is easy to cause blood vessel ingrowth and fibrosis repair. The preparation method comprises the following steps: inoculating cartilage cells on a three-dimensional porous frame structure carrier, carrying out in-vitro dynamic pre-culture to enable the cartilage cells to secrete an extracellular matrix, and carrying out decellularization treatment to obtain the functionalized cartilage tissue repair material retaining the cartilage specific matrix. The material has a suitable pore structure and abundant II-type collagen deposition, can effectively promote chondrocyte differentiation and hyaline cartilage regeneration, inhibits blood vessel ingrowth, maintains a cartilage vascular-free microenvironment, significantly improves the structure and function reconstruction effect of cartilage defect repair, and has good biocompatibility and clinical transformation potential.

According to the application of the triple-network hydrogel to preparation of the osteochondral defect repair material, a PRP-coated OSA-GEL three-network composite hydrogel system is provided based on PRP, OSA and gelatin, in the system, a dynamic covalent network of OSA and gelatin, a physical network of fibrin and an egg box model formed between Ca < 2 + > and OSA form an IPN network with enhanced mechanical properties, and the PRP-coated OSA-GEL three-network composite hydrogel system has the advantages that the mechanical properties of the PRP-coated OSA-GEL three-network composite hydrogel system are enhanced; a dynamic cross-linking system of the PRP-coated OSA-GEL hydrogel not only can support cell infiltration and tissue regeneration, but also brings self-repairing capability to the hydrogel, so that a hydrogel network can be quickly recovered after the hydrogel is broken, the vulnerable biological activity of PRP is protected, new cartilage of the PRP-coated OSA-GEL is complete, regenerated cartilage tissue in a defect area is almost completely fused with surrounding normal cartilage, and the defect area of the PRP-coated OSA-GEL is not damaged. It is indicated that the PRP remarkably enhances the cartilage regeneration effect through PRP-coated OSA-GEL, and the PRP has effectiveness in the aspect of promoting and enhancing osteochondral regeneration.

The application discloses a multi-component synovitis treatment composition based on repairing cartilage tissue and application thereof, and relates to the technical field of biological medicine, and the composition is composed of glucosamine hydrochloride, chondroitin sulfate, dimethyl sulfone and graded molecular weight sodium hyaluronate; the application precisely compounding the graded molecular weight sodium hyaluronate with the glucosamine hydrochloride, the chondroitin sulfate and the dimethyl sulfone according to weight parts, constructs a gradient slow-release and target delivery system of synovitis lesions, specifically inhibits the activity of hyaluronidase in the inflammatory joint cavity by using the dimethyl sulfone, greatly reduces the degradation of the sodium hyaluronate, prolongs the residence time of the active ingredients at the lesion site, realizes immediate analgesia and relieves joint stiffness by quickly forming a lubricating protective film by the high molecular weight sodium hyaluronate, the medium and low molecular weight sodium hyaluronate penetrates the synovial membrane barrier and carries the cartilage repair components to enrich in the cartilage defect area, and the synergistic effect of the components is realized.

The present disclosure relates to a method of treating arthritis by targeting Tankyrase. The methods according to the present disclosure can be advantageously used for regeneration of cartilage tissue and for treating osteoarthritis by maximizing the matrix synthesis in cartilage by inhibition of Tankyrase and regulation of other proteins related therewith.

The application discloses a kind of targeted delivery molecules, particle and its preparation method, application, comprising the following steps: step 1: to the polyethylene glycol molecule A solution containing maleimide group and hydrophobic group is added dropwise with thiol-containing polypeptide targeting factor B solution, stir and react fully;Wherein the amount-of-substance ratio of A and B is 1:1;Step 2: the solution obtained in step 1 is dialyzed, freeze-dried to obtain the required targeted delivery molecules;The targeted delivery molecules prepared by the application have cartilage cell-specific polypeptide targeting factor at one end, and can target cartilage tissue.The hydrophobic group at the other end can be inserted into the hydrophobic lipid bilayer by physical action.The polypeptide targeting factor is assembled on the surface of cell, cell exosome, liposome and other vesicles composed of lipid bilayer, which has the effect of treating osteoarthritis.The above-mentioned cells and vesicles can be targeted to deliver the contents to cartilage, and have good application prospect in the treatment of osteoarthritis.

This invention provides a hybrid exosome nanomotor driven by a pathological microenvironment, its preparation method, and its applications, belonging to the field of biomedical technology. The hybrid exosome nanomotor provided by this invention can utilize the unique oxidative microenvironment of the osteoarthritis lesion area to achieve self-driven movement, thereby actively and efficiently penetrating the dense extracellular matrix of cartilage tissue, significantly enhancing its enrichment and retention capacity in the lesion area, and promoting the specific uptake of therapeutic molecules by chondrocytes. This system not only achieves intelligent response and utilization to the disease microenvironment but also simultaneously alleviates local oxidative stress, ultimately achieving efficient, precise, and sustained therapeutic effects. This invention provides a novel, proactively targeted delivery strategy and treatment platform with promising clinical translation prospects for diseases such as osteoarthritis involving dense tissues or inflammatory microenvironments.

The invention relates to a method for extracting chondroitin sulfate sodium by a one-step method through superposition of multiple complex enzymes and specific trypsin, which comprises the following steps: S1, cleaning animal cartilage tissue, freeze-drying, and crushing into powder; s2, adding the powder into a buffer solution of a compound enzyme formed by combining specific trypsin 201, recombinant protease 017, lipase, cellulase, pancreatin and neutral protease, and carrying out enzymolysis reaction at 40 DEG C for 4 hours; s3, after the enzymolysis reaction is finished, performing centrifugal separation, ethanol precipitation, filtration, washing and drying to obtain a sodium chondroitin sulfate product. According to the extraction method disclosed by the invention, through the synergistic effect of various complex enzymes, cartilage tissues can be degraded more sufficiently, the yield of sodium chondroitin sulfate is improved, the loss of an intermediate extraction step is reduced by a one-pot method, and the extraction rate of a final product reaches 17% and is 10% higher than that of a traditional extraction rate.

The invention relates to a tissue scaffold developed for use in the treatment of cartilage tissue loss and a method for its production.

The invention relates to the technical field of modern biomedicine, in particular to a method for promoting differentiation of human umbilical cord mesenchymal stem cells based on an EVs composite material, which comprises the following steps: 1, preparing a double-layer composite scaffold; 2, preparation and characterization of EVs: primary cartilage cell culture, enzymatic digestion of cartilage tissue, digestion termination and cell collection, EVs extraction, and EVs characterization identification; 3, loading the EVs; 4, separation, identification and differentiation induction of the hUC-MSCs; and 5, differentiation effect detection. The method has the characteristics that the differentiation efficiency and stability are good, the EVs delivery efficiency is remarkably improved, the biocompatibility and safety are good, the operation is simple and convenient, the cost is controllable, and the application scene is flexible and wide.

This invention discloses an injectable porous hydrogel microsphere containing magnesium and targeting cartilage, its preparation method, and its application. The preparation method is as follows: Step 1, preparing phosphorylated methacrylamide gelatin; Step 2, thoroughly mixing the product from Step 1 with magnesium oxide nanoparticles and a photoinitiator to form an aqueous phase, and then preparing MgO-GelMA-PS microspheres with an oil phase using a microfluidic device; Step 3, reacting the hydrogel microspheres from Step 2 with a chitosan quaternary ammonium salt solution to obtain M-GP@QCS. The hydrogel microspheres of this invention possess phosphate groups, which can provide binding sites to form stable metal ligand supramolecular bonds with MgO NPs, achieving continuous release of magnesium ions and long-term therapeutic effects on osteoarthritis; a chitosan quaternary ammonium salt coating is constructed on the surface of the microspheres, and the positive charge of the coating binds to the negatively charged cartilage tissue, achieving targeted adhesion of the microspheres to cartilage and realizing precise treatment of osteoarthritis.

The invention provides a hybrid exosome nano motor based on pathological microenvironment driving and a preparation method and application thereof, and belongs to the technical field of biological medicine. The hybrid exosome nano motor provided by the invention can realize self-driven movement by utilizing a specific oxidation microenvironment of an osteoarthritis lesion area, so that compact extracellular matrixes of cartilage tissues can be actively and efficiently penetrated, the enrichment and retention capacities of the motor in the lesion area are remarkably improved, and specific uptake of therapeutic molecules by cartilage cells is promoted. The system not only realizes intelligent response and utilization to a disease microenvironment, but also synchronously relieves local oxidative stress, and finally achieves an efficient, accurate and continuous treatment effect. The invention provides a brand-new active targeting delivery strategy and treatment platform with a good clinical transformation prospect for diseases related to dense tissues or inflammatory microenvironments, such as osteoarthritis.

Developed is a method for creating cartilage tissue to solve the following problems. 1. Cartilage differentiation is not hindered by forming. 2. The cartilage can be formed into an intended shape without using a scaffold.　A method for producing artificial cartilage tissue comprises: forming a spheroid containing cartilage precursor cells into a desired shape while seeding the spheroid onto a support; culturing the spheroid while supplying a culture medium from the front side and the rear side of the surface onto which the spheroid has been seeded, and fusing the spheroids with each other; and maturing the fused spheroid into cartilage tissue in vitro.

The present invention discloses a multi-component synovitis therapeutic composition based on cartilage tissue repair and a use thereof, relating to the technical field of biomedicine. The composition comprises glucosamine hydrochloride, chondroitin sulfate, dimethyl sulfone, and graded molecular weight sodium hyaluronate. By compounding these components in precise parts by weight, the present invention constructs a gradient sustained-release and targeted delivery system for synovitis lesions. Dimethyl sulfone specifically inhibits hyaluronidase activity in the inflamed joint cavity, significantly reducing sodium hyaluronate degradation and prolonging the retention time of each active ingredient at the lesion site. High molecular weight sodium hyaluronate rapidly forms a lubricating protective film to achieve immediate analgesia and relieve ankylosis, while medium- and low-molecular-weight sodium hyaluronate penetrates the synovial barrier and carries cartilage repair components to the cartilage defect area, thereby achieving synergistic enhancement and comprehensively improving synovitis treatment efficacy.

This invention relates to the field of biomaterials technology, specifically to a mesoporous bioactive enhanced cartilage adhesion hydrogel and its preparation method. The preparation method is as follows: Ethyl acetate, ammonia solution, tetraethyl orthosilicate, and calcium nitrate are added to a hexadecyltrimethylammonium bromide dispersion, and the mixture is stirred at 55-65°C for 5-8 hours to obtain mesoporous bioactive glass nanoparticles (MBGNs). Highly reactive AG is prepared using gelatin, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, and N-hydroxysuccinimide as raw materials. 2-8% (w / w) of MBGNs is added to a DAS solution to obtain a DAS / MBGNs mixed solution, and then the AG solution is added and stirred thoroughly to obtain the mesoporous bioactive enhanced cartilage adhesion hydrogel (ADGM). The prepared ADGM hydrogel provides stable support and stress dispersion, and also achieves covalent adhesion at the cartilage tissue interface and regulation of the biological microenvironment, realizing a synergistic unity of structural stability and functional repair.

The invention relates to the technical field of biological materials and tissue engineering, and discloses a 3D printed gradient layered anisotropic bionic cartilage scaffold and a preparation method thereof. On the macroscopic level, the 3D-printed porous structure with gradient change is provided, so that the pore size of each layer can be properly regulated and controlled, and the space structure of surrounding primary cartilage tissue can be properly matched after the 3D-printed porous structure is implanted into a body. And secondly, on the microscopic level, the cartilage tissue culture medium has different-layer anisotropic orientation structures for template freezing, and can guide directional differentiation and functional expression of cartilage cells at different layers, so that the regeneration and repair process of cartilage tissues is accelerated. Therefore, the bionic cartilage scaffold disclosed by the invention can fully simulate the microenvironment of natural cartilage, and plays an important role in repairing and regenerating damaged cartilage tissues.

The invention relates to the technical field of knee joint repair, and particularly provides a stem cell preparation for knee joint injury repair as well as a preparation method and application thereof. The NGF monoclonal antibody 4G13 and the bone marrow mesenchymal stem cells are combined for knee joint injury repair for the first time, and test analysis shows that the preparation can effectively reduce the levels of IL-1beta, TNF-alpha and IL-6 in serum of a patient suffering from gonitis, inhibit secretion of the IL-1beta, the TNF-alpha and the IL-6, repair knee joint inflammatory injury, improve and promote cartilage tissue repair, and have a good application prospect in knee joint injury repair. Good application prospects are realized.

The invention relates to liquid modified milk and a preparation method thereof, the liquid modified milk comprises animal milk, glucosamine hydrochloride, calcium beta-hydroxy-beta-methylbutyrate and cordyceps militaris extract, the cordyceps militaris extract contains cordycepin and adenosine, the cordycepin content in the liquid modified milk is greater than or equal to 1.6 mg / 100mL, and the adenosine content in the liquid modified milk is greater than or equal to 0.6 mg / 100mL. According to the modified milk provided by the invention, cordycepin enhances the biosynthesis and energy supply capability of mitochondria by activating related signal channels, and provides an energy basis for the GaHMB to play a role, so that the synthesis efficiency of muscle protein is improved; adenosine and glucosamine have a synergistic effect, so that conduction of inflammation-related signals can be blocked, and decomposition of cartilage matrixes is reduced; meanwhile, glucosamine is used as a glycosaminoglycan precursor, under the action of a signal channel regulated and controlled by adenosine, the synthesis efficiency of cartilage tissue can be remarkably stimulated, the utilization efficiency of sugar is improved, and finally triple synergistic intervention of muscle-joint-metabolism is achieved.

The invention provides antibacterial and antioxidant lignin composite hydrogel as well as a preparation method and application thereof.The antibacterial and antioxidant lignin composite hydrogel is prepared by the steps that acrylamide and acrylic acid are subjected to a cross-linking reaction under the action of N, N-methylene bisacrylamide and an initiator, and a hydrogel matrix is formed; after sodium lignin sulfonate is introduced, hydrogel with an interpenetrating network structure is formed; after tannic acid and zinc chloride are introduced, the antibacterial and antioxidant lignin composite hydrogel which is complex and compact in structure is formed. The hydrogel has relatively strong oxidation resistance, antibacterial property and self-repairing property, can be used as a medical dressing, assists in repairing wounds and avoids inflammation of the wounds; meanwhile, the hydrogel can also be used as a cartilage tissue material, and self-repairing of the cartilage tissue can be achieved through the dynamic reversible coordination effect among zinc chloride, tannic acid and a hydrogel matrix.

The invention provides a Midkine-overexpressed mesenchymal stem cell, an extracellular vesicle and application of the Midkine-overexpressed mesenchymal stem cell and the extracellular vesicle. The mesenchymal stem cell is integrated with a gene element for overexpressing Midkine, the long-term survival and tissue chemotaxis ability of the mesenchymal stem cell are utilized to realize local continuous overexpression of Midkine recombinant protein, the engineered mesenchymal stem cell integrates the advantages of the Midkine recombinant protein and the mesenchymal stem cell, the synergistic effect of the Midkine recombinant protein and the mesenchymal stem cell is exerted, and the engineering mesenchymal stem cell has the advantages of promoting tissue regeneration and repair, promoting tissue regeneration and repairing and improving tissue regeneration and repair efficiency. Particularly, the method has huge potential in the aspects of cartilage tissue regeneration and skin wound repair; besides, the extracellular vesicles secreted by the Midkine-overexpressed mesenchymal stem cells contain Midkine recombinant protein or mRNA (messenger Ribonucleic Acid), and are also used for promoting cartilage injury repair.

The application discloses a kind of imitated plant phloem layer drug-loading slow-release bionic cartilage scaffold and preparation method thereof.The scaffold includes hollow porous fiber three-dimensional network formed by cross-stacked hollow porous fiber body;Including drug-loading culture solution layer filled with hollow area;Including bone marrow mesenchymal stem cells wrapped in the gap between the outside.The method comprises the following steps: three-dimensional printing of polydimethylsiloxane (PDMS) composite ink is carried out by direct writing three-dimensional printing platform to obtain hollow porous fiber three-dimensional network;After high-temperature curing, water bath heating and high-temperature high-pressure sterilization, rinse, then immerse in cell culture solution for culture, then inoculate bone marrow mesenchymal stem cells for culture, to obtain drug-loading slow-release bionic cartilage scaffold.The application can match the mechanical properties of native cartilage, and has the functions of slow-release drug to surrounding tissue to kill bacteria and anti-inflammatory, and promote the regeneration of surrounding cartilage tissue, promote the gradual repair of damaged cartilage tissue.

The utility model discloses a cryopreserved autologous cartilage tissue stem cell transport kit, which comprises a main body unit and a fetching unit, the main body unit comprises a box body, the top of the box body is rotatably connected with a box cover, the bottom of the box body is provided with a first chamber, the box body is internally provided with a second chamber, and the second chamber is communicated with the first chamber. A plurality of storage cavities and a plurality of limiting grooves are further formed in the box body, the storage cavities communicate with the limiting grooves, the limiting grooves are located above the storage cavities, the bottoms of the storage cavities communicate with the second cavity, and a third cavity is formed in the top of the box body. When the drawer is moved out of the first cavity, the drawer moves to drive the rotating rod to rotate, the rotating rod rotates to drive the supporting plate to move upwards, and when the supporting plate moves upwards, the reagent bottles can be driven to move upwards, so that the tops of the reagent bottles are driven to move out of the containing cavity, and then the reagent bottles are taken out conveniently; and the ice bag in the ice bag can be conveniently taken out for replacement.

The application relates to a biological 3D printed tissue engineering trachea and a construction method and application thereof, namely a cartilage / vascularized fiber tissue alternating tissue engineering trachea. The construction method comprises the following steps: firstly, cartilage specific biological ink is prepared by wrapping cartilage cells with cartilage tissue specific hydrogel; fiber specific biological ink is prepared by wrapping fibroblasts with fiber tissue specific hydrogel; then, through a double-needle extrusion type biological 3D printing technology, alternating printing is realized to form a ring-shaped tubular structure, and a cartilage / fiber tissue alternating tubular structure is constructed through multi-layer accumulation molding; further, vascularization of the fiber tissue is realized through a certain culture mode, and finally, a relatively mature cartilage / vascularized fiber tissue alternating tissue engineering trachea is obtained. The application also provides application of the cartilage / vascularized fiber tissue alternating tissue engineering trachea in tracheal defect repair.

The invention provides a precise layering sterile sampling device for articular cartilage tissues. The precise layering sterile sampling device is characterized by comprising an equipment base, a mounting base, a measuring unit, a first retracting unit and a second propelling unit, the device disclosed by the invention can be used for carrying out micron-sized precise stratified sampling on articular cartilage tissues under a sterile operation condition. According to the equipment, the effect of accurately sampling layered tissues of fresh active articular cartilage is achieved, and a foundation is laid for deep research on the layered tissue structure, mechanics and layered cells of the articular cartilage.

The application discloses a method for semi-quantitative analysis of expression level of secreted protein of human joint cartilage cells by using immunoblotting technology. The method comprises the following steps: 1) preparing a secreted protein culture solution; 2) collecting a sample of the secreted protein culture solution; 3) performing dialysis desalting treatment on the sample; 4) concentrating the secreted protein culture solution to prepare a powder; 5) re-dissolving the secreted protein culture solution powder and quantifying the protein; 6) separating the protein components in the re-dissolved culture solution; and 7) detecting the expression level of the secreted protein in the re-dissolved culture solution sample by using immunoblotting method. The low-temperature dialysis and vacuum concentration technology can significantly improve the concentration of the secreted protein in the human joint cartilage tissue culture solution sample under the condition of maintaining the integrity of the protein structure, and is suitable for detecting the chemical fluorescence signal of the secreted protein in the sample by using the immunoblotting technology. The application provides an important cell reaction detection tool for studying the pathogenesis of bone and joint diseases caused by degenerative changes of cartilage tissue and researching and developing biological small-molecule drugs for treating the diseases.

The invention discloses injectable porous hydrogel microspheres of magnesium-containing targeting cartilage as well as a preparation method and application of the injectable porous hydrogel microspheres. The preparation method comprises the following steps: step 1, preparing phosphorylated methacrylamide gelatin; 2, fully mixing a product obtained in the step 1 with magnesium oxide nanoparticles and a photoinitiator to form a water phase, and preparing MgO-GelMA-PS microspheres from the water phase and an oil phase through a microfluidic device; and 3, carrying out contact reaction on the hydrogel microspheres obtained in the step 2 and a chitosan quaternary ammonium salt solution to obtain the M-GP (at) QCS. The hydrogel microspheres provided by the invention have phosphate groups, and can provide binding sites to form stable metal ligand supramolecular binding with MgO NPs, so that magnesium ions are continuously released, and the effect of treating osteoarthritis for a long time is realized; a chitosan quaternary ammonium salt coating is constructed on the surface of the microsphere, and positive charges of the coating are combined with cartilage tissues with negative charges, so that the effect that the microsphere adheres to cartilage in a targeted manner is achieved, and the effect of accurately treating osteoarthritis is achieved.

The present application belongs to the field of biomedical technology, and relates to a berberine / chondrocyte / injectable hydrogel biomimetic material, a preparation method and application thereof. The present application combines phosphoric acid modified methylacrylate chitosan injectable hydrogel (scaffold material), chondrocyte (seed cell) and berberine (bioactive substance) into one to form a berberine / chondrocyte / injectable hydrogel biomimetic cartilage scaffold material. The material can realize long-time effect of berberine on chondrocyte, is prepared into a cartilage scaffold, can act on residual chondrocyte and new chondrocyte around the cartilage scaffold, realizes regeneration and repair of cartilage tissue on the basis of climbing material provided by the hydrogel, and has a "1+1+1>3" effect, thereby providing a new strategy for cartilage defect treatment.

The invention belongs to the technical field of biomedical materials, and particularly relates to a highly-bionic gradient artificial cartilage scaffold material, a preparation method and application, the highly-bionic gradient artificial cartilage scaffold material comprises a hyaline cartilage layer, a calcified cartilage layer and a subchondral bone layer, the calcified cartilage layer is composed of mineralized I-type collagen fibers containing 30% of nano-hydroxyapatite, and the subchondral bone layer is composed of mineralized I-type collagen fibers containing 60% of nano-hydroxyapatite; the prepared scaffold highly simulates natural osteochondral tissues, has similar composition, nano structure and mechanical properties with the natural osteochondral tissues, and presents an obvious gradient; the hydrogel has excellent biocompatibility and biological activity, and cell proliferation and gradient differentiation of bone marrow mesenchymal stem cells can be remarkably promoted; in a rabbit osteochondral defect model, osteochondral tissue regeneration is remarkably promoted, and the compound can be applied to clinical treatment of osteoarthritis.

The present invention relates to a composition for the prevention or treatment of rheumatoid arthritis, comprising, as an active ingredient, a supercritical extract of Acanthopanax sessiliflorus Harms. In the present invention, it was confirmed that, upon administration of the supercritical extract of Acanthopanax sessiliflorus Harms to a rheumatoid arthritis animal model, pain induced by rheumatoid arthritis was reduced and cartilage damage was suppressed. Also, a decrease in the infiltration of immune cells in cartilage tissue was confirmed, and it was confirmed that the expression of Col2a1, Sox9, and Aggrecan, which are cartilage anabolic factors whose expression is reduced by inflammatory cytokines and inflammatory factors, was increased, and the expression of Mmp3, Mmp10, Mmp13, and Adamts5, which are cartilage catabolic factors, was reduced. It was also confirmed that pimaric acid and kaurenoic acid, which are useful components of the supercritical extract of Acanthopanax sessiliflorus Harms, protected chondrocytes and suppressed the differentiation of Th17 cells, which are pathogenic cells in rheumatoid arthritis.