55 results about "Cartilage tissues" patented technology

The invention relates to application of a miR-155-5p inhibitor delivered by an exosome of a targeted synovitis macrophage to treatment of osteoarthritis, and belongs to the technical field of biological medicines. According to the scheme, the exosome drug delivery system for targeting the synovial inflammatory macrophages in the osteoarthritis, which contains the miR-155-5p inhibitor, is prepared through a genetic engineering technology, so that the exosome derived from stem cells is targeted to inflammatory synovial tissues, and damaged cartilage tissues are repaired and inflammation is resisted based on the stem cell exosome; compared with the prior art, the application has the advantages that the combined action of inhibiting the osteoarthritis injury and inhibiting the miR-155-5p so as to resist synovitis is realized, the osteoarthritis injury repair can be better applicable, a new scheme for treating the osteoarthritis is provided, a support is provided for clinical transformation, and the application value is extremely high.

The invention belongs to the technical field of biological medicines, and discloses a B cell and cartilage cell senescence targeting osteoarthritis treatment medicine. The invention provides an application of an anti-MIF antibody or an antigen binding fragment thereof as a B cell targeting immunomodulator in osteoarthritis treatment drugs, the anti-MIF antibody or the antigen binding fragment thereof regulates and controls an MIF / HMGB1 / MMP13 signal axis through a neutralization effect with MIF, so that the MIF and B cells are significantly co-localized, expression of HMGB1 in the B cells is promoted, and the osteoarthritis treatment effect is improved. The senescence of B cells is reduced and the B cells are promoted to regulate or repair phenotype transformation so as to destroy chronic inflammatory circulation and diffusion of pathogenic plasma cells, and the expression of MMP13 in joint tissues is reduced so as to maintain the steady state of cartilage tissues; the preparation method has an excellent application prospect in preparation of an OA intra-articular injection drug which realizes rapid anti-inflammatory and long-term cartilage protection effects and can realize a lasting curative effect through low-frequency drug delivery.

The invention relates to polyglutamic acid bio-ink with antibacterial and antioxidant functions as well as a preparation method and application of the polyglutamic acid bio-ink. The method comprises the following steps: firstly, degrading high-molecular-weight gamma-polyglutamic acid at a specific high temperature to obtain degraded gamma-polyglutamic acid (d gamma-PGA) which is low in viscosity and suitable for printing; then, the gamma-polyglutamic acid (d gamma-PGAGMA) is reacted with a methacrylic acid acylation reagent, and the photo-crosslinkable gamma-polyglutamic acid (d gamma-PGAGMA) degraded by methylacryloylation is prepared; and finally, mixing the dgamma-PGAGMA, a photoinitiator and the living cells to obtain the biological ink. The hydrogel is suitable for 3D biological printing and can be formed at will, and the hydrogel formed through printing has the effects of inhibiting bacteria survival and resisting oxidation and can promote growth of cartilage cells and regeneration of cartilage tissue.

The present invention relates to a method for producing cartilage tissue by selecting and choosing from chondrocytes or spheroids, and use thereof as pharmaceutical composition, medicinal product or transplant. In particular, the invention relates to markers for identifying suitable chondrocytes for producing cartilage tissue, in particular for the purpose of transplantation.

Large volume cellular material may be preserved by combining the cellular material with a cryoprotectant formulation / medium / solution containing at least one mNP and then subjecting the cellular material to a vitrification preservation protocol including nanowarming. This preservation method is particularly effective for cartilage tissues.

The present invention discloses an integrated bone and cartilage scaffold with a directional pore structure and a preparation method thereof, which belongs to the fields of tissue engineering, biomedicine and biomanufacturing technology. The method simulates the natural material and structure of bone cartilage, uses the principle of directional crystallization to prepare a bone and cartilage scaffold with a directional structure, and uses a low-temperature deposition manufacturing process based on rapid prototyping technology to prepare a horizontally oriented cartilage layer scaffold and a vertically oriented subchondral bone layer scaffold. Between the cartilage layer and the subchondral bone layer, methacrylated gelatin capable of UV cross-linking is combined with other materials with chemical grafting and bonding effects as a transition layer, thereby preparing a tissue engineering bone and cartilage integrated composite scaffold. The present invention is aimed at the treatment of articular bone and cartilage defects, and uses a bioactive material to manufacture a bone and cartilage composite scaffold with a directional pore structure. It can promote the coordinated repair of bone and cartilage tissue according to the characteristics of different functional areas of bone and cartilage, thereby improving the repair effect of bone and cartilage defects.

The invention relates to a functionalized cartilage tissue repair material as well as a preparation method and application thereof, and belongs to the technical field of tissue engineering. The invention aims to solve the problems that the existing decalcified bone matrix is insufficient in cartilage induction in cartilage repair, and is easy to cause blood vessel ingrowth and fibrosis repair. The preparation method comprises the following steps: inoculating cartilage cells on a three-dimensional porous frame structure carrier, carrying out in-vitro dynamic pre-culture to enable the cartilage cells to secrete an extracellular matrix, and carrying out decellularization treatment to obtain the functionalized cartilage tissue repair material retaining the cartilage specific matrix. The material has a suitable pore structure and abundant II-type collagen deposition, can effectively promote chondrocyte differentiation and hyaline cartilage regeneration, inhibits blood vessel ingrowth, maintains a cartilage vascular-free microenvironment, significantly improves the structure and function reconstruction effect of cartilage defect repair, and has good biocompatibility and clinical transformation potential.

According to the application of the triple-network hydrogel to preparation of the osteochondral defect repair material, a PRP-coated OSA-GEL three-network composite hydrogel system is provided based on PRP, OSA and gelatin, in the system, a dynamic covalent network of OSA and gelatin, a physical network of fibrin and an egg box model formed between Ca < 2 + > and OSA form an IPN network with enhanced mechanical properties, and the PRP-coated OSA-GEL three-network composite hydrogel system has the advantages that the mechanical properties of the PRP-coated OSA-GEL three-network composite hydrogel system are enhanced; a dynamic cross-linking system of the PRP-coated OSA-GEL hydrogel not only can support cell infiltration and tissue regeneration, but also brings self-repairing capability to the hydrogel, so that a hydrogel network can be quickly recovered after the hydrogel is broken, the vulnerable biological activity of PRP is protected, new cartilage of the PRP-coated OSA-GEL is complete, regenerated cartilage tissue in a defect area is almost completely fused with surrounding normal cartilage, and the defect area of the PRP-coated OSA-GEL is not damaged. It is indicated that the PRP remarkably enhances the cartilage regeneration effect through PRP-coated OSA-GEL, and the PRP has effectiveness in the aspect of promoting and enhancing osteochondral regeneration.

Embodiments relate to an imidazolidine derivative or a pharmaceutically acceptable salt thereof and its use for preventing, ameliorating, or treating arthritis. The compound of one embodiment can be effectively utilized for preventing, ameliorating, or treating arthritis by inhibiting degeneration or damage to cartilage tissue through inhibition of the activity of ADAMTS4 or ADAMTS5.

The application discloses a multi-component synovitis treatment composition based on repairing cartilage tissue and application thereof, and relates to the technical field of biological medicine, and the composition is composed of glucosamine hydrochloride, chondroitin sulfate, dimethyl sulfone and graded molecular weight sodium hyaluronate; the application precisely compounding the graded molecular weight sodium hyaluronate with the glucosamine hydrochloride, the chondroitin sulfate and the dimethyl sulfone according to weight parts, constructs a gradient slow-release and target delivery system of synovitis lesions, specifically inhibits the activity of hyaluronidase in the inflammatory joint cavity by using the dimethyl sulfone, greatly reduces the degradation of the sodium hyaluronate, prolongs the residence time of the active ingredients at the lesion site, realizes immediate analgesia and relieves joint stiffness by quickly forming a lubricating protective film by the high molecular weight sodium hyaluronate, the medium and low molecular weight sodium hyaluronate penetrates the synovial membrane barrier and carries the cartilage repair components to enrich in the cartilage defect area, and the synergistic effect of the components is realized.

The application discloses a kind of bionic bone / cartilage double-layer composite scaffold material and its preparation method and application.The bionic bone / cartilage double-layer composite scaffold material of the application is composed of upper cartilage repair hydrogel layer and lower bone repair scaffold layer.The main feature of the application is that by radial freezing, the chitosan-chitin whisker liquid crystal composite hydrogel micron radial channel with diameter gradient change is constructed in the lower scaffold, which can promote the transport of nutrients, cell recruitment, migration and adhesion;At the same time, the drug-loaded liquid crystal hydrogel constructed in the longitudinal large channel of the scaffold and the upper cartilage repair layer can realize the sustained release of active drugs, so as to further regulate stem cell osteogenesis / cartilage differentiation and promote the generation of blood vessels, and is expected to have good application prospect as biomedical materials such as bone / cartilage tissue repair materials.

The present disclosure relates to a method of treating arthritis by targeting Tankyrase. The methods according to the present disclosure can be advantageously used for regeneration of cartilage tissue and for treating osteoarthritis by maximizing the matrix synthesis in cartilage by inhibition of Tankyrase and regulation of other proteins related therewith.

The present invention discloses a method for extracting chondroitin sulfate by enzymatic-deep eutectic solvent coupling, comprising the steps of extracting chondroitin sulfate from animal cartilage tissue raw material using a protease-assisted deep eutectic solvent, wherein the protease comprises one or both of a serine protease and a metalloprotease, and the raw materials for preparing the deep eutectic solvent include betaine and glucose. The present invention further claims protection for a chondroitin sulfate product prepared based on the protease-assisted deep eutectic solvent method for extracting chondroitin sulfate. The present invention utilizes a protease-assisted deep eutectic solvent to extract chondroitin sulfate, greatly improving both extraction efficiency and extraction purity, and the extraction method is environmentally friendly.

The application discloses a kind of targeted delivery molecules, particle and its preparation method, application, comprising the following steps: step 1: to the polyethylene glycol molecule A solution containing maleimide group and hydrophobic group is added dropwise with thiol-containing polypeptide targeting factor B solution, stir and react fully;Wherein the amount-of-substance ratio of A and B is 1:1;Step 2: the solution obtained in step 1 is dialyzed, freeze-dried to obtain the required targeted delivery molecules;The targeted delivery molecules prepared by the application have cartilage cell-specific polypeptide targeting factor at one end, and can target cartilage tissue.The hydrophobic group at the other end can be inserted into the hydrophobic lipid bilayer by physical action.The polypeptide targeting factor is assembled on the surface of cell, cell exosome, liposome and other vesicles composed of lipid bilayer, which has the effect of treating osteoarthritis.The above-mentioned cells and vesicles can be targeted to deliver the contents to cartilage, and have good application prospect in the treatment of osteoarthritis.

The invention belongs to the technical field of biological materials and tissue engineering, and relates to functionalized silk fibroin-based bio-ink, bionic cartilage hydrogel and a preparation method thereof. The ink contains an initiator, SFMA, GelMA, PEGDA and OBC, and the ratio of all the components is strictly controlled; after the ink is uniformly mixed with stem cells, a curing cross-linking reaction is initiated, and the multi-bionic cartilage hydrogel is prepared. The hydrogel comprises hydrogel with a multi-network structure as well as OBC and stem cells which are simultaneously dispersed in the hydrogel with the multi-network structure, and has a compression modulus matched with a real cartilage tissue and a porous structure. The functionalized silk fibroin-based bio-ink and the multiple bionic cartilage hydrogel provided by the invention solve the problems of insufficient donors, immunological rejection, single bionic dimension, unmatched mechanical properties and the like in the prior art, and can realize high-precision printing and efficiently promote cartilage regeneration.

This invention provides a hybrid exosome nanomotor driven by a pathological microenvironment, its preparation method, and its applications, belonging to the field of biomedical technology. The hybrid exosome nanomotor provided by this invention can utilize the unique oxidative microenvironment of the osteoarthritis lesion area to achieve self-driven movement, thereby actively and efficiently penetrating the dense extracellular matrix of cartilage tissue, significantly enhancing its enrichment and retention capacity in the lesion area, and promoting the specific uptake of therapeutic molecules by chondrocytes. This system not only achieves intelligent response and utilization to the disease microenvironment but also simultaneously alleviates local oxidative stress, ultimately achieving efficient, precise, and sustained therapeutic effects. This invention provides a novel, proactively targeted delivery strategy and treatment platform with promising clinical translation prospects for diseases such as osteoarthritis involving dense tissues or inflammatory microenvironments.

The invention provides a construction method of a growth disorder mouse model based on a Cre / LoxP cartilage tissue conditional knockout Ankrd11 gene, and belongs to the technical field of biology. The construction method disclosed by the invention comprises the following steps: enabling an Ankrd11fl / fl mouse to be mated with a Col2a1-CreERT2 tool mouse, so as to obtain Ankrd11fl / wt; the mouse is a Col2a1-CreERT2 mouse; the Ankrd < 11 > fl / wt; the Col2a1-CreERT2 mouse and the Ankrd11fl / fl mouse are subjected to mating, and the Ankrd11fl / fl mouse is obtained, and the Col2a1-CreERT2 mouse and the Ankrd11fl / fl mouse are subjected to mating; a Col2a1-CreERT2 mouse is subjected to tamoxifen induction treatment, and the growth disorder mouse model is obtained. The construction method provides an animal experiment model for further researching the pathogenic mechanism of the KBG syndrome growth disorder phenotype.

The invention relates to a method for extracting chondroitin sulfate sodium by a one-step method through superposition of multiple complex enzymes and specific trypsin, which comprises the following steps: S1, cleaning animal cartilage tissue, freeze-drying, and crushing into powder; s2, adding the powder into a buffer solution of a compound enzyme formed by combining specific trypsin 201, recombinant protease 017, lipase, cellulase, pancreatin and neutral protease, and carrying out enzymolysis reaction at 40 DEG C for 4 hours; s3, after the enzymolysis reaction is finished, performing centrifugal separation, ethanol precipitation, filtration, washing and drying to obtain a sodium chondroitin sulfate product. According to the extraction method disclosed by the invention, through the synergistic effect of various complex enzymes, cartilage tissues can be degraded more sufficiently, the yield of sodium chondroitin sulfate is improved, the loss of an intermediate extraction step is reduced by a one-pot method, and the extraction rate of a final product reaches 17% and is 10% higher than that of a traditional extraction rate.

The invention relates to a tissue scaffold developed for use in the treatment of cartilage tissue loss and a method for its production.

The application relates to the field of biological medicines, in particular to a cartilage tissue repair hydrogel and a preparation method thereof. The preparation components of the cartilage tissue repair hydrogel are doped with 10-15 g of mulberry silk, 0.1-0.5 g of glycidyl methacrylate, 0-1 g of lithium magnesium silicate, 0-1 g of tannic acid and 0.1 g of a photo initiator per 100 ml of the hydrogel. The preparation method of the cartilage tissue repair hydrogel is as follows: degumming and dissolving treatment are conducted on the mulberry silk to obtain a silk fibroin solution, glycidyl methacrylate is added into the silk fibroin solution and stirring is conducted to obtain a methacrylated silk fibroin solution, lithium magnesium silicate solution and tannic acid solution are added into the methacrylated silk fibroin solution to obtain a silk fibroin mixed solution, and then the photo initiator is added into the silk fibroin mixed solution to prepare the cartilage tissue repair hydrogel. The tissue repair hydrogel has a hydrophilic three-dimensional network structure, can provide mechanical support and biological induction activity for a bone cartilage defect site, and is suitable for bone injury repair.

The utility model provides a conical grinding drill, which relates to the technical field of medical instruments and comprises a grinding drill bit, a connecting rod and a joint operation power device. The grinding drill bit is conical or truncated cone-shaped, and the small-diameter end of the end face of the grinding drill bit is connected with one end of the connecting rod and used for grinding cartilage tissue and bone tissue of the abraded joint through the large-diameter end of the end face of the grinding part; the other end of the connecting rod is connected with a joint operation power device which is used for driving the connecting rod to rotate, and then the grinding drill bit is driven to rotate through the connecting rod. According to the conical grinding drill, the conical or circular-truncated-cone-shaped grinding drill bit is arranged at one end of the connecting rod, cartilage tissue and bone tissue of a worn joint are ground through the end, with the large diameter, of the end face of the grinding drill bit, and the section diameter of the grinding drill bit at an operative incision is small; and the rotating space required to be reserved for the grinding drill bit is small, so that the grinding drill bit can grind in a smaller surgical incision.

The application provides a kind of cartilage polypeptide composition and its preparation method, the cartilage polypeptide 30-45%, auxiliary agent 10-25%, growth agent 3-8%, slow-release agent 2-9%, the balance is water, the application will cartilage polypeptide, auxiliary agent, growth agent and slow-release agent several ingredients scientific compatibility, preparation has the composition of protecting cartilage tissue, anti-inflammatory pain, effectively increase bone density effect, improve synovial tissue, there is no rebound ineffective condition after drug withdrawal.

The utility model discloses an autologous chondrocyte culture device for cartilage tissue repair, which comprises a culture device body and a culture shelf body, the culture device body comprises a box body, and a constant temperature control system is arranged in the box body; the culture shelf body comprises a plurality of placing plates, the placing plates are detachably mounted in the box body, a plurality of vent holes are formed in the placing plates, a plurality of connecting blocks are rotatably connected to the inner sides of the left side wall and the right side wall of the box body, sliding rails are arranged in the connecting blocks, and the sliding rails are arranged in the sliding rails. And sliding blocks are fixedly connected to the left end and the right end of the placement plate. The placing plate is rotatably mounted in the culture device, and when the culture dish needs to shake, the culture dish can be driven to shake through rotation of the placing plate, so that the shake of the culture dish does not need to be taken out from the culture device, and the culture dish is in a constant-temperature environment while being conveniently shaken; the culture of cartilage cells is facilitated.

The invention relates to the technical field of modern biomedicine, in particular to a method for promoting differentiation of human umbilical cord mesenchymal stem cells based on an EVs composite material, which comprises the following steps: 1, preparing a double-layer composite scaffold; 2, preparation and characterization of EVs: primary cartilage cell culture, enzymatic digestion of cartilage tissue, digestion termination and cell collection, EVs extraction, and EVs characterization identification; 3, loading the EVs; 4, separation, identification and differentiation induction of the hUC-MSCs; and 5, differentiation effect detection. The method has the characteristics that the differentiation efficiency and stability are good, the EVs delivery efficiency is remarkably improved, the biocompatibility and safety are good, the operation is simple and convenient, the cost is controllable, and the application scene is flexible and wide.

This invention discloses an injectable porous hydrogel microsphere containing magnesium and targeting cartilage, its preparation method, and its application. The preparation method is as follows: Step 1, preparing phosphorylated methacrylamide gelatin; Step 2, thoroughly mixing the product from Step 1 with magnesium oxide nanoparticles and a photoinitiator to form an aqueous phase, and then preparing MgO-GelMA-PS microspheres with an oil phase using a microfluidic device; Step 3, reacting the hydrogel microspheres from Step 2 with a chitosan quaternary ammonium salt solution to obtain M-GP@QCS. The hydrogel microspheres of this invention possess phosphate groups, which can provide binding sites to form stable metal ligand supramolecular bonds with MgO NPs, achieving continuous release of magnesium ions and long-term therapeutic effects on osteoarthritis; a chitosan quaternary ammonium salt coating is constructed on the surface of the microspheres, and the positive charge of the coating binds to the negatively charged cartilage tissue, achieving targeted adhesion of the microspheres to cartilage and realizing precise treatment of osteoarthritis.

The invention provides a hybrid exosome nano motor based on pathological microenvironment driving and a preparation method and application thereof, and belongs to the technical field of biological medicine. The hybrid exosome nano motor provided by the invention can realize self-driven movement by utilizing a specific oxidation microenvironment of an osteoarthritis lesion area, so that compact extracellular matrixes of cartilage tissues can be actively and efficiently penetrated, the enrichment and retention capacities of the motor in the lesion area are remarkably improved, and specific uptake of therapeutic molecules by cartilage cells is promoted. The system not only realizes intelligent response and utilization to a disease microenvironment, but also synchronously relieves local oxidative stress, and finally achieves an efficient, accurate and continuous treatment effect. The invention provides a brand-new active targeting delivery strategy and treatment platform with a good clinical transformation prospect for diseases related to dense tissues or inflammatory microenvironments, such as osteoarthritis.

The present invention relates to an extracellular vesicle mass production method using a cell spheroid embedded with a microparticle having a differentiation stimulating factor supported therein, and the extracellular vesicle obtained through the production method has the effect of regenerating a tissue, in particular, a cartilage tissue, and, thus, can be used to treat diseases related to tissue regeneration and cartilage by utilizing such effect.

Developed is a method for creating cartilage tissue to solve the following problems. 1. Cartilage differentiation is not hindered by forming. 2. The cartilage can be formed into an intended shape without using a scaffold.　A method for producing artificial cartilage tissue comprises: forming a spheroid containing cartilage precursor cells into a desired shape while seeding the spheroid onto a support; culturing the spheroid while supplying a culture medium from the front side and the rear side of the surface onto which the spheroid has been seeded, and fusing the spheroids with each other; and maturing the fused spheroid into cartilage tissue in vitro.

The present invention discloses a multi-component synovitis therapeutic composition based on cartilage tissue repair and a use thereof, relating to the technical field of biomedicine. The composition comprises glucosamine hydrochloride, chondroitin sulfate, dimethyl sulfone, and graded molecular weight sodium hyaluronate. By compounding these components in precise parts by weight, the present invention constructs a gradient sustained-release and targeted delivery system for synovitis lesions. Dimethyl sulfone specifically inhibits hyaluronidase activity in the inflamed joint cavity, significantly reducing sodium hyaluronate degradation and prolonging the retention time of each active ingredient at the lesion site. High molecular weight sodium hyaluronate rapidly forms a lubricating protective film to achieve immediate analgesia and relieve ankylosis, while medium- and low-molecular-weight sodium hyaluronate penetrates the synovial barrier and carries cartilage repair components to the cartilage defect area, thereby achieving synergistic enhancement and comprehensively improving synovitis treatment efficacy.

This invention relates to the field of biomaterials technology, specifically to a mesoporous bioactive enhanced cartilage adhesion hydrogel and its preparation method. The preparation method is as follows: Ethyl acetate, ammonia solution, tetraethyl orthosilicate, and calcium nitrate are added to a hexadecyltrimethylammonium bromide dispersion, and the mixture is stirred at 55-65°C for 5-8 hours to obtain mesoporous bioactive glass nanoparticles (MBGNs). Highly reactive AG is prepared using gelatin, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, and N-hydroxysuccinimide as raw materials. 2-8% (w / w) of MBGNs is added to a DAS solution to obtain a DAS / MBGNs mixed solution, and then the AG solution is added and stirred thoroughly to obtain the mesoporous bioactive enhanced cartilage adhesion hydrogel (ADGM). The prepared ADGM hydrogel provides stable support and stress dispersion, and also achieves covalent adhesion at the cartilage tissue interface and regulation of the biological microenvironment, realizing a synergistic unity of structural stability and functional repair.