45 results about "Cartilage lesion" patented technology

The present application relates to chondrocyte compositions and kits comprising morselized cartilage tissue particles and a biodegradable matrix. Applications and methods of using the chondrocyte composition for cartilage grafts, repairing cartilage injuries and defects, and joint repair are provided.

The invention belongs to the field of biological medicines, and particularly relates to application of loganic acid in preparation of a medicine for treating arthritis. Specifically, through experimental research of a collagen-induced arthritis mouse model, the loganin acid is found to be capable of remarkably relieving arthritis symptoms, relieving joint swelling, synovitis and cartilage injury in a dose-dependent manner, and reducing the levels of proinflammatory factors TNF-alpha and IL-6 in plasma. By further combining 16S rRNA sequencing analysis, the loganic acid can remarkably regulate the intestinal microbiota structure of arthritis mice, increase the abundance of Lactobacillus murinus in the intestinal tract and promote the generation of tryptophan metabolite indole-3-acetic acid, so that the effect of resisting rheumatoid arthritis is achieved through an intestinal flora dependent mechanism. Therefore, the invention provides a new application of loganin acid in preparation of a medicine for treating arthritis, and the compound has further research and application values as a potential small molecule medicine.

The invention provides a traditional Chinese medicine composition, a traditional Chinese medicine preparation, a preparation method and application, and particularly belongs to the technical field of traditional Chinese medicine. The invention provides a traditional Chinese medicine composition. The traditional Chinese medicine composition comprises the following components in parts by weight: 20-50 parts of parched white peony root, 8-15 parts of pawpaw, 10-20 parts of radix clematidis, 20-40 parts of caulis spatholobi, 5-12 parts of honey-fried licorice root, 10-20 parts of fructus broussonetiae, 10-20 parts of radix cyathulae and 5-12 parts of cassia twig. The traditional Chinese medicine composition has great potential in the aspects of treating kOA and inhibiting osteophyte formation, and can be used for improving kOA cartilage injury. Through in-vitro experiments, network pharmacology and in-vivo experiments, the curative effect of XBD in treating kOA and the effect of XBD in inhibiting osteophyte formation are verified, it is proved that XBD has a regulation effect on cartilage degeneration and osteophyte formation, and an important thought is provided for developing innovative drugs with cartilage protection and abnormal osteophyte formation inhibition functions.

The invention relates to the technical field of biological medicines, and particularly discloses application of emetine hydrochloride in preparation of a medicine for treating osteoarthritis. A cell test shows that the chondrocyte activity can be remarkably improved by an epecinol hydrochloride alkaline solution; animal model tests show that the epecinol hydrochloride alkali solution can maintain the steady state of chondrocyte metabolism, significantly reduce the cartilage injury degree of osteoarthritis mice and relieve osteoarthritis, and provides a new way for improving the osteoarthritis condition. The chemical structural formula of the emetine hydrochloride is shown in the specification.

The present invention provides a mechanoelectrically coupled hydrogel microsphere with stable mechanical properties and low-loss transduction, as well as a preparation method and application thereof, belonging to the technical field of tissue engineering biorepair materials. The present invention utilizes supramolecular engineering and microfluidics technology to introduce a slip-ring structured polyrotaxane and a conductive polypyrrole network to construct stress-electrically coupled hydrogel microspheres. The molecular slip mechanism of the slip-ring structure stores and releases mechanical energy, reducing mechanical losses, while the conjugated π-electron movement in the conductive network improves the efficiency of electron transfer inside the microspheres, thereby reducing the losses in stress-electric conversion for the first time. Compared with traditional piezoelectric hydrogel microspheres, the mechanoelectrically coupled hydrogel microspheres of the present invention have a stress-electric coupling efficiency of 2.3 times higher than that of traditional piezoelectric hydrogel microspheres, and the energy dissipation rate is reduced to 43%. The mechanoelectrically coupled hydrogel microspheres can alleviate cartilage damage in rats, improve behavioral outcomes, and promote the treatment of osteoarthritis by restoring low-loss transduction between tissues.

The invention discloses application of guaiacol in treatment of rheumatoid arthritis, and particularly relates to application of guaiacol in preparation of drugs for treating rheumatoid arthritis. Cell experiments prove that guaiacol can effectively down-regulate expression of proinflammatory factors in macrophages and up-regulate expression of anti-inflammatory factors, and has remarkable anti-inflammatory activity; animal experiments show that the guaiacol effectively relieves rheumatoid arthritis model mouse diseases, and the specific manifestation is that the guaiacol obviously relieves the mouse claw swelling degree, reduces the arthritis disease score, obviously improves joint inflammatory cell infiltration, repairs cartilage injury, inhibits osteoclast generation and reduces the number of joint apoptotic cells; meanwhile, the level of proinflammatory factors in serum of mice can be effectively reduced, and the level of proinflammatory repair factors can be up-regulated. The invention proves that guaiacol has a definite curative effect on rheumatoid arthritis, good biological safety and no obvious side effect, provides a new effective active component for the treatment of rheumatoid arthritis, and has a good prospect of being developed into the medicine for treating rheumatoid arthritis.

Hydrolyzed collagen compositions are described which are used for treatment of impaired cartilage or to prevent damage to healthy cartilage. These compositions function across multiple cartilage components and structures. Oral, topical, or cartilage-site delivery of the compositions to cartilage may be via liquid, gel, powder, or sheet form. Additionally, the hydrolyzed collagen composition may be applied as a coating on a medical device.

This invention discloses a method for preparing a 3D-printed hydrogel scaffold for cartilage repair, comprising: providing methacrylamide chitosan; providing 2-([1,1-biphenyl]-4-ylcarbamoyl)benzoic acid@polylactic acid-glycolic acid copolymer microspheres; obtaining a 3D-printed bio-ink for cartilage repair using methacrylamide chitosan, 2-([1,1-biphenyl]-4-ylcarbamoyl)benzoic acid@polylactic acid-glycolic acid copolymer microspheres, recombinant collagen CF-1552, and lithium magnesium silicate as main raw materials; and obtaining a 3D-printed bio-ink for cartilage repair using the 3D-printed bio-ink for cartilage repair as a raw material to obtain a 3D-printed hydrogel scaffold for cartilage repair. This 3D-printed hydrogel scaffold has advantages such as antibacterial properties and the ability to promote the differentiation of human bone marrow mesenchymal stem cells (hBMSCs) into chondrocytes, and can be used for cartilage repair, potentially bringing new curative opportunities for cartilage injury diseases.

The invention relates to an Apt / DNA-PPK hydrogel sustained-release system as well as a preparation method and application thereof. The Apt / DNA-PPK hydrogel sustained release system is characterized in that an aptamer (Apt-DNA) DNA hydrogel (Apt-DNA) with a stem cell capturing function is combined with dopamine nanoparticles with an NIR response release function. Wherein the premix solution of the Apt / DNA-PPK hydrogel sustained release system contains Apt-DNA, PDA (Personal Digital Assistant), KGN (Kinyl Glycol Nitride) and PEI (Polyether Ionium). Compared with the prior art, the Apt / DNA-PPK hydrogel sustained-release system disclosed by the invention has the advantages that free BMSCs (bone marrow mesenchymal stem cells) in the joint are captured through the cell capturing function of the Apt 19S. Meanwhile, under the irradiation of NIR, the Apt / DNA-PPK hydrogel releases the loaded KGN, and the KGN differentiates the BMSCs captured by the hydrogel into cartilage cells, so that the problem of cartilage injury in the early stage of osteoarthritis is solved, and an innovative strategy and material selection are provided for tissue engineering.

The invention relates to the technical field of biological medicines, and particularly discloses application of adenosine monophosphate in preparation of a medicine for treating osteoarthritis. Cell tests show that the adenosine monophosphate solution has an inhibition effect on cartilage cell activity; animal model tests show that the adenosine monophosphate normal saline solution can remarkably relieve the cartilage injury degree of osteoarthritis mice and relieve osteoarthritis, and a new way is provided for improvement of osteoarthritis conditions. The chemical structural formula of adenosine monophosphate is shown in the specification.

The invention belongs to the technical field of biological medicines, and particularly relates to application of xanthosine monophosphate in preparation of a medicine for treating osteoarthritis. The molecular formula of the xanthosine monophosphate is C10H13N4O9P, and the structure of the xanthosine monophosphate is shown in the specification. Research results show that the flavin monophosphate solution has an inhibition effect on cartilage cell activity; animal model tests show that the flavin monophosphate normal saline solution can remarkably relieve the cartilage injury degree of osteoarthritis mice and relieve osteoarthritis, and a new way is provided for improvement of osteoarthritis conditions. .

The invention discloses a mesenchymal stem cell compound preparation for treating cartilage injury, and belongs to the technical field of cell biology. The composite preparation consists of human umbilical cord mesenchymal stem cells (hUC-MSC) subjected to specific pretreatment and a pharmaceutical carrier. The pretreatment method sequentially comprises the following steps: carrying out low-oxygen pretreatment on the hUC-MSC; then, carrying out pretreatment on a ferroptosis inhibitor Ferrostatin-1 under a low-oxygen condition; and finally, carrying out icariin pretreatment under a normal oxygen condition. The unique triple pretreatment strategy generates a remarkable synergistic effect, and can greatly improve the survival rate and the function maintenance capability of the hUC-MSC in the cartilage injury inflammatory microenvironment. Experimental results prove that the preparation can significantly inhibit collagen degradation and promote cartilage matrix regeneration, and the treatment efficiency of stem cell transplantation and the repair effect of cartilage injury are effectively improved.

This invention provides a stress-triggered selective targeting hydrogel microsphere, its preparation method, and its application, belonging to the field of biomedical technology. The core of this invention is the "stress-triggered charge reversal" mechanism. It utilizes the interaction between weakly cross-linked disulfide bonds broken under stress and "charge reversal" messenger molecules to achieve "charge reversal" of nanoparticles under stress. A dual-network hydrogel microsphere system is prepared using microfluidic technology to achieve bearing lubrication and stress relaxation within the joint cavity. Stress-triggered charge reversal is achieved at stress-overloaded cartilage lesions, imparting a positive charge to the drug-loaded nanoparticles within the hydrogel microspheres. This significantly enhances the ability of the drug-loaded nanoparticles to penetrate deep into the cartilage matrix and target chondrocytes for drug release under the guidance of the charge. This enables the selective and targeted release of appropriate drug concentrations to deep-seated chondrocytes in pathological microenvironments induced by different stress intensities, achieving highly effective and low-toxicity treatment of osteoarthritis.

The present application relates to a kind of polypeptide for cartilage repair and its composition and application, belong to bone injury repair technical field.The polypeptide is polypeptide PG155, and the experimental results of the present application show that it can repair cartilage damage caused by osteoarthritis to some extent.Further research shows that the special exosome (PG155-Exo) produced by polypeptide PG155 pretreatment bone marrow mesenchymal stem cells combined with polypeptide PG155, can significantly enhance the therapeutic effect, and show obvious synergistic effect.This synergistic effect makes the cartilage repair effect significantly better than single therapy, and provides more efficient treatment scheme for osteoarthritis patients.

This invention relates to a method for constructing a humanized pig model of type II collagen and its application, belonging to the field of gene modification technology. The invention first provides a CRISPR-Cas9 system for targeted editing of the porcine type II collagen α1 gene. Using CRISPR / Cas9-mediated homologous recombination, the porcine COL2A1 gene is orally replaced with the human COL2A1 gene. Then, using somatic cell cloning technology, a humanized pig model of type II collagen is constructed, allowing the pig to produce cartilage tissue more similar to that of humans, reducing post-transplant immune rejection. This is expected to fundamentally solve the problem of human cartilage tissue shortage and has significant implications for the treatment of articular cartilage damage, providing a better and more suitable transplant material for the treatment of articular cartilage damage.

The invention relates to regenerated cartilage based on hydrostatic pressure stimulation and a preparation method and application thereof. The method comprises the following steps: inoculating cells on a porous hydrogel scaffold, placing the porous hydrogel scaffold in a culture medium, performing pressure stimulation by adopting a hydrostatic pressure bioreactor, and performing in-vitro culture for a period of time to obtain the regenerated cartilage based on hydrostatic pressure stimulation. Key mechanical sensitive channels TRPV4 and PIEZO1 are up-regulated through hydrostatic pressure stimulation, a Ca < 2 + > dependent TRPV4 / PIEZO1-Ca < 2 + >-SOX9 mechanical transduction pathway is activated, cartilage formation is promoted, and hypertrophy and ossification are inhibited. Through verification, the bioreactor system is combined with the porous hydrogel scaffold, has good biocompatibility, controllable pore structure and mechanical property, and is suitable for cartilage cell culture. After in-vitro pre-culture for 4 weeks, the cartilage-like construct subjected to HP pretreatment shows a long-term stable regeneration effect in an animal model (such as a goat), and especially in an in-situ auricle microenvironment, inflammation and heterotopic ossification can be effectively inhibited, and an efficient in-vitro cartilage engineering strategy is provided for repairing of various cartilage injuries.

The present invention relates to a biomaterial comprising: —a membrane wound patch (a), made of a nanofibrous polymeric scaffold, —a hydrogel (b) including autologous or allogenic bone marrow-derived mesenchymal stem cells, and —a bone wound patch (c) being a nanofibrous scaffold made of polymers, wherein said scaffold has a surface coated with an interrupted coating made of multilayered droplets, said multilayered droplets being droplets composed of at least one layer pair consisting of a layer of polyanions and a layer of polycations, and wherein the bone wound patch (c) further comprises a bone growth factor; wherein the hydrogel (b) is included between the membrane wound patch (a) and the bone wound patch (c).

Hydrolyzed collagen compositions are described which are used for treatment of impaired cartilage or to prevent damage to healthy cartilage. These compositions function across multiple cartilage components and structures. Oral, topical, or cartilage-site delivery of the compositions to cartilage may be via liquid, gel. powder, or sheet form. Additionally, the hydrolyzed collagen composition may be applied as a coating on a medical device.

A lubricin-localized cartilage-like tissue and a method for producing the same, wherein, when in an arbitrary cross section passing a first center of mass of a cartilage-like tissue derived from pluripotent stem cells or a center-of-mass region, which is a portion inside a concentric sphere being centered at the first center of mass and having a diameter of [first major diameter×0.2], the expression level of lubricin per unit area contained in a central region, which is a portion inside a concentric circle being centered at a second center of mass that is the center of mass of the cross section and having a diameter of [major diameter of cross section (second major diameter)×(0.4 to 0.9)] is referred to as the central lubricin level and the expression level of lubricin per unit area contained in the non-central region is referred to as the non-central lubricin level.

The utility model relates to a pusher for cartilage injury repair under an arthroscope, which belongs to the technical field of medical instruments and comprises a push rod and an outer sleeve, the push rod is movably arranged in the outer sleeve and is in interference fit with the outer sleeve, and the tail end of the outer sleeve is connected with a handle convenient to hold; an inclined groove is formed in the head end of the outer sleeve, the outer sleeve comprises a semi-groove pipe, and the semi-groove pipe is used for guiding other auxiliary instruments to conduct an operation; according to the pusher for the cartilage injury repair operation under the arthroscope, repair materials are pushed to a designated injury position through cooperation of the push rod and the outer sleeve, the head end of the outer sleeve is designed to be of an inclined groove structure so that laying and operation of the cartilage materials in the operation can be facilitated, and the semi-groove pipe is arranged to guide other auxiliary instruments to conduct the operation; according to the utility model, the surgical operation is more convenient and quicker, the repair material can be effectively prevented from being damaged, and the surgical efficiency is improved.

A method for enhancing cartilage regeneration following its wounding is provided which includes identifying a mammal as experiencing an injury to its cartilage applying to it a composition including graphene oxide (GO) and hyaluronic acid (HA) that are covalently linked, XAV939, and water. The composition of the method may also include a surfactant, such as PEG. The composition can be topically administered to a subject to treat cartilage wounding of the subject.

The present invention relates to a complex for promoting cartilage differentiation comprising stem cells or a culture thereof and a cartilage cell-free crush, a method for fabricating cartilage by using the complex, cartilage fabricated via the method, a pharmaceutical composition comprising the complex for preventing or treating arthropathy, a method for preventing or treating arthropathy using the composition, a composition for inducing cartilage cell differentiation comprising the prepared cartilage cells, and a method for fabricating cartilage using the prepared cartilage cells. The complex for promoting cartilage differentiation comprising the stem cells or the culture thereof and the cartilage cell-free crush provided in the present invention is not only differentiated into cartilage cells without side effects even in vivo, but also promotes differentiation of inherent stem cells in vivo into cartilage cells via a paracrine effect exhibited in the differentiated cartilage cells to effectively regenerate the damaged cartilage, and thus the complex may be widely used for treatment of arthropathy involving the cartilage damage.

Provided are dual-affinity fusion proteins including an affibody domain specific for a therapeutic protein, and including a localization domain specific for a structural bone component. The therapeutic protein can include bone morphogenetic protein 2 (BMP-2), vascular endothelial growth factor (VEGF), fibroblast growth factor 2 (FGF-2), platelet-derived growth factor (PDGF), granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-4 (IL-4), or glial derived neurotrophic factor (GDNF). Also provided are compositions that include the dual-affinity fusion proteins, affibodies and the corresponding therapeutic proteins, and / or a medical material used to treat a wound, or a bone or cartilage injury or disease. Also provided are methods of using the compositions, for example to treat bone injuries, bone diseases, cartilage injuries, cartilage diseases, and wounds. In some examples, the composition includes at least two different dual-affinity fusion proteins specific for the same therapeutic protein, but have different disassociation constants (KD).

This invention relates to the fields of regenerative medicine and neuromodulation technology, specifically disclosing the application of mediators that inhibit the activity of the auditory cortex in the preparation of drugs or intervention devices for promoting cartilage regeneration, repairing cartilage damage, and delaying or treating osteoarthritis. This invention promotes cartilage damage repair and osteoarthritis treatment by modulating the auditory cortex of the auditory center, providing two technical means: a chemogenetic method involving targeted injection of a viral vector carrying an artificial inhibitory receptor into the auditory cortex followed by administration of a DREADD agonist; and a sound stimulation method involving outputting sound stimulation to the individual at a level 5 dB higher than the ambient background noise. Animal experiments have confirmed that both methods can upregulate the expression of cartilage synthesis markers Col2, Acan, and PRG4, downregulate the expression of degradation markers Col1 and Mmp13, improve histological scores, and alleviate pain. This invention also provides an intervention device, including a sound insulation unit, a sound acquisition unit, a control unit, and a sound output unit. This device has the advantages of being non-invasive, easy to operate, and highly safe, providing an innovative strategy for cartilage regeneration and osteoarthritis treatment.

Disclosed is a method of treating or preventing skeletal sequelae of coronavirus infection in a subject in need thereof, the method comprising administering to the subject a therapeutically effective amount of an endothelin receptor antagonist, with the proviso that the endothelin receptor antagonist is not co-administered with dapagliflozin or niclosamide. The skeletal sequelae may include one or more of arthralgia, viral arthritis, osteopenia, osteochondral injury, and osteoporosis.

The invention discloses nanoparticles and an electromagnetic transfection system for repairing cartilage injury as well as a preparation method and application of the nanoparticles and the electromagnetic transfection system, and belongs to the technical field of biological medicines. The preparation method of the nanoparticles comprises the following steps: S1, carrying out amination modification on SPIONs coated with silicon dioxide, so as to obtain aminated SPIONs; and S2, covalently binding small interfering RNA to the surface of the aminated SPIONs obtained in the S1 through a cross-linking agent, thereby obtaining the product. The si-SIRT3 (at) SPIONs nano delivery system is successfully constructed, non-invasive EMF exposure is combined, the problem that the cartilage tissue transfection efficiency is low is solved, SIRT3 overexpression, primary cilia injury and cartilage matrix degradation caused by factors such as T-2 toxin can be effectively reversed, the obvious cartilage protection and repair effects are shown in vitro and in vivo, and the cartilage tissue transfection system has the advantages of being high in biocompatibility, good in biocompatibility and good in biocompatibility. A novel targeted therapy normal form is provided for treating cartilage injury diseases such as Kaschin-Beck disease.

The invention discloses an application of a halophenol compound 2, 4 ', 5'-trihydroxy-5, 2 '-dibromo benzophenone as a medicament for resisting rheumatoid arthritis. The compound can effectively relieve the ankle joint swelling degree of rheumatoid arthritis rats, relieve cartilage injury and reduce the arthritis score, main treatment indexes such as MMP-9, IL-17A, IL-1beta and IgM are all superior to those of a clinical medicine celecoxib, the reduction of the spleen coefficient is remarkably superior to that of celecoxib, and the important application prospect in the aspect of treating rheumatoid arthritis is shown.

The invention provides a Midkine-overexpressed mesenchymal stem cell, an extracellular vesicle and application of the Midkine-overexpressed mesenchymal stem cell and the extracellular vesicle. The mesenchymal stem cell is integrated with a gene element for overexpressing Midkine, the long-term survival and tissue chemotaxis ability of the mesenchymal stem cell are utilized to realize local continuous overexpression of Midkine recombinant protein, the engineered mesenchymal stem cell integrates the advantages of the Midkine recombinant protein and the mesenchymal stem cell, the synergistic effect of the Midkine recombinant protein and the mesenchymal stem cell is exerted, and the engineering mesenchymal stem cell has the advantages of promoting tissue regeneration and repair, promoting tissue regeneration and repairing and improving tissue regeneration and repair efficiency. Particularly, the method has huge potential in the aspects of cartilage tissue regeneration and skin wound repair; besides, the extracellular vesicles secreted by the Midkine-overexpressed mesenchymal stem cells contain Midkine recombinant protein or mRNA (messenger Ribonucleic Acid), and are also used for promoting cartilage injury repair.

The present invention relates to a hydrogel composition prepared without using a binder. The composition is easy to inject into the human body and has a therapeutic effect on intraarticular lesions and inflammation. The hydrogel of the present invention, which is prepared without a binder, is degraded in an articulation for a long time due to the increased viscoelasticity thereof, thereby enjoying the advantage that the absorption time is prolonged. Therefore, when the hydrogel is used as an injection, its efficacy may be maintained for a long time. Furthermore, the hydrogel has no cytotoxicity, thus showing very high biocompatibility. Particularly when directly injected to an injured site in an articulation, the hydrogel has a therapeutic effect on the intraarticular lesion and inflammation in the corresponding site and thus may be advantageously utilized as a composition for prevention or treatment of an intraarticular lesion and as a viscosity supplement.