60 results about "Cartilage lesion" patented technology

The present application relates to chondrocyte compositions and kits comprising morselized cartilage tissue particles and a biodegradable matrix. Applications and methods of using the chondrocyte composition for cartilage grafts, repairing cartilage injuries and defects, and joint repair are provided.

The invention belongs to the field of biological medicines, and particularly relates to application of loganic acid in preparation of a medicine for treating arthritis. Specifically, through experimental research of a collagen-induced arthritis mouse model, the loganin acid is found to be capable of remarkably relieving arthritis symptoms, relieving joint swelling, synovitis and cartilage injury in a dose-dependent manner, and reducing the levels of proinflammatory factors TNF-alpha and IL-6 in plasma. By further combining 16S rRNA sequencing analysis, the loganic acid can remarkably regulate the intestinal microbiota structure of arthritis mice, increase the abundance of Lactobacillus murinus in the intestinal tract and promote the generation of tryptophan metabolite indole-3-acetic acid, so that the effect of resisting rheumatoid arthritis is achieved through an intestinal flora dependent mechanism. Therefore, the invention provides a new application of loganin acid in preparation of a medicine for treating arthritis, and the compound has further research and application values as a potential small molecule medicine.

The invention relates to the technical field of medicines, foods and health-care products, in particular to application of morusin in preparation of a product for treating osteoarthritis. A hydrogen peroxide induced chondrocyte in-vitro model and a D-galactose subcutaneous injection osteoarthritis animal model are used for screening and evaluating the morusin, and it is found that the morusin has the obvious effects of relieving articular cartilage injury, enhancing articular cartilage toughness, delaying the course of osteoarthritis and the like. Wide application prospects are realized in the aspect of preparing products for treating osteoarthritis.

The invention provides a traditional Chinese medicine composition, a traditional Chinese medicine preparation, a preparation method and application, and particularly belongs to the technical field of traditional Chinese medicine. The invention provides a traditional Chinese medicine composition. The traditional Chinese medicine composition comprises the following components in parts by weight: 20-50 parts of parched white peony root, 8-15 parts of pawpaw, 10-20 parts of radix clematidis, 20-40 parts of caulis spatholobi, 5-12 parts of honey-fried licorice root, 10-20 parts of fructus broussonetiae, 10-20 parts of radix cyathulae and 5-12 parts of cassia twig. The traditional Chinese medicine composition has great potential in the aspects of treating kOA and inhibiting osteophyte formation, and can be used for improving kOA cartilage injury. Through in-vitro experiments, network pharmacology and in-vivo experiments, the curative effect of XBD in treating kOA and the effect of XBD in inhibiting osteophyte formation are verified, it is proved that XBD has a regulation effect on cartilage degeneration and osteophyte formation, and an important thought is provided for developing innovative drugs with cartilage protection and abnormal osteophyte formation inhibition functions.

The invention relates to the technical field of biological medicines, and particularly discloses application of emetine hydrochloride in preparation of a medicine for treating osteoarthritis. A cell test shows that the chondrocyte activity can be remarkably improved by an epecinol hydrochloride alkaline solution; animal model tests show that the epecinol hydrochloride alkali solution can maintain the steady state of chondrocyte metabolism, significantly reduce the cartilage injury degree of osteoarthritis mice and relieve osteoarthritis, and provides a new way for improving the osteoarthritis condition. The chemical structural formula of the emetine hydrochloride is shown in the specification.

The present invention provides a mechanoelectrically coupled hydrogel microsphere with stable mechanical properties and low-loss transduction, as well as a preparation method and application thereof, belonging to the technical field of tissue engineering biorepair materials. The present invention utilizes supramolecular engineering and microfluidics technology to introduce a slip-ring structured polyrotaxane and a conductive polypyrrole network to construct stress-electrically coupled hydrogel microspheres. The molecular slip mechanism of the slip-ring structure stores and releases mechanical energy, reducing mechanical losses, while the conjugated π-electron movement in the conductive network improves the efficiency of electron transfer inside the microspheres, thereby reducing the losses in stress-electric conversion for the first time. Compared with traditional piezoelectric hydrogel microspheres, the mechanoelectrically coupled hydrogel microspheres of the present invention have a stress-electric coupling efficiency of 2.3 times higher than that of traditional piezoelectric hydrogel microspheres, and the energy dissipation rate is reduced to 43%. The mechanoelectrically coupled hydrogel microspheres can alleviate cartilage damage in rats, improve behavioral outcomes, and promote the treatment of osteoarthritis by restoring low-loss transduction between tissues.

The invention discloses application of guaiacol in treatment of rheumatoid arthritis, and particularly relates to application of guaiacol in preparation of drugs for treating rheumatoid arthritis. Cell experiments prove that guaiacol can effectively down-regulate expression of proinflammatory factors in macrophages and up-regulate expression of anti-inflammatory factors, and has remarkable anti-inflammatory activity; animal experiments show that the guaiacol effectively relieves rheumatoid arthritis model mouse diseases, and the specific manifestation is that the guaiacol obviously relieves the mouse claw swelling degree, reduces the arthritis disease score, obviously improves joint inflammatory cell infiltration, repairs cartilage injury, inhibits osteoclast generation and reduces the number of joint apoptotic cells; meanwhile, the level of proinflammatory factors in serum of mice can be effectively reduced, and the level of proinflammatory repair factors can be up-regulated. The invention proves that guaiacol has a definite curative effect on rheumatoid arthritis, good biological safety and no obvious side effect, provides a new effective active component for the treatment of rheumatoid arthritis, and has a good prospect of being developed into the medicine for treating rheumatoid arthritis.

The present invention discloses the use of Histatin-1 polypeptide in promoting cartilage regeneration or repair. By preparing a combined reagent composed of Histatin-1 polypeptide, a biological scaffold material, etc., mainly by loading linear Hst1 on gelatin methacrylate, it is used for cartilage injury repair or promoting cartilage growth. At the same time, the use of Histatin-1 polypeptide in promoting the expression of one or more of the bone differentiation indicators, including collagen fibers, glycosaminoglycans, type II collagen, and aggrecan, is also disclosed.

The invention provides an application of an Akt pathway activator in treatment of cartilage injury and systemic reaction caused by joint hemorrhage. Knee joint hemorrhage modeling is carried out on mice and pigs; it is found that the Akt pathway activator sc79 and vitamin E have remarkable curative effects in treatment of cartilage injury, systemic reaction, liver injury and peripheral blood inflammation caused by joint bleeding, and a new target and a new treatment means are provided for prevention and treatment of joint bleeding.

The present invention relates to a pharmaceutical composition for treating cartilage damage diseases, comprising, as an active ingredient, magnetic nanoparticle-embedded nasal septal chondrocytes , and establishes a method for embedding magnetic nanoparticles into nasal septal chondrocytes and a method for preparing spheroids by culturing magnetic nanoparticle-embedded nasal septal chondrocytes. It was identified that the magnetic nanoparticle-embedded nasal septal chondrocytes prepared by the method of the present invention and spheroids prepared therefrom exhibit cartilage damage treatment activity while maintaining mobility in a magnetic field. In addition, since the activity is maintained even if spheroids are prepared by mixing magnetic nanoparticle-embedded nasal septal chondrocytes and nasal septal chondrocytes, the present invention can be effectively used as a composition for treating cartilage damage diseases that has an excellent therapeutic effect without causing side effects by having a significantly reduced addition ratio of the magnetic nanoparticles.

Hydrolyzed collagen compositions are described which are used for treatment of impaired cartilage or to prevent damage to healthy cartilage. These compositions function across multiple cartilage components and structures. Oral, topical, or cartilage-site delivery of the compositions to cartilage may be via liquid, gel, powder, or sheet form. Additionally, the hydrolyzed collagen composition may be applied as a coating on a medical device.

This invention discloses a method for preparing a 3D-printed hydrogel scaffold for cartilage repair, comprising: providing methacrylamide chitosan; providing 2-([1,1-biphenyl]-4-ylcarbamoyl)benzoic acid@polylactic acid-glycolic acid copolymer microspheres; obtaining a 3D-printed bio-ink for cartilage repair using methacrylamide chitosan, 2-([1,1-biphenyl]-4-ylcarbamoyl)benzoic acid@polylactic acid-glycolic acid copolymer microspheres, recombinant collagen CF-1552, and lithium magnesium silicate as main raw materials; and obtaining a 3D-printed bio-ink for cartilage repair using the 3D-printed bio-ink for cartilage repair as a raw material to obtain a 3D-printed hydrogel scaffold for cartilage repair. This 3D-printed hydrogel scaffold has advantages such as antibacterial properties and the ability to promote the differentiation of human bone marrow mesenchymal stem cells (hBMSCs) into chondrocytes, and can be used for cartilage repair, potentially bringing new curative opportunities for cartilage injury diseases.

The invention relates to the technical field of cell preparations, in particular to a method for rapidly evaluating the repairing function of cartilage cells on cartilage injury at low cost, which comprises the following steps: constructing a cartilage injury model by adopting pig knee and femoral joint cartilage tissues, inoculating cell spheres formed by the to-be-evaluated cartilage cells to an injury part of the cartilage injury model, and evaluating the cartilage injury part of the cartilage injury model. And transplanting the cartilage cells into an experimental animal body, and evaluating the repair function of the cartilage cells according to the repair condition of the cartilage injury model. The method provides a systematic scheme for evaluating the repairing function of the cartilage cells, has the advantages of simplicity in operation, short time consumption, low cost, high stability and the like, and provides an effective method for evaluating the functions of the cartilage cells and screening and evaluating the functions of cells such as induced pluripotent stem cells with cartilage injury repairing potential; the method has a good application prospect in development of cell preparations for cartilage injury repair.

The invention relates to an Apt / DNA-PPK hydrogel sustained-release system as well as a preparation method and application thereof. The Apt / DNA-PPK hydrogel sustained release system is characterized in that an aptamer (Apt-DNA) DNA hydrogel (Apt-DNA) with a stem cell capturing function is combined with dopamine nanoparticles with an NIR response release function. Wherein the premix solution of the Apt / DNA-PPK hydrogel sustained release system contains Apt-DNA, PDA (Personal Digital Assistant), KGN (Kinyl Glycol Nitride) and PEI (Polyether Ionium). Compared with the prior art, the Apt / DNA-PPK hydrogel sustained-release system disclosed by the invention has the advantages that free BMSCs (bone marrow mesenchymal stem cells) in the joint are captured through the cell capturing function of the Apt 19S. Meanwhile, under the irradiation of NIR, the Apt / DNA-PPK hydrogel releases the loaded KGN, and the KGN differentiates the BMSCs captured by the hydrogel into cartilage cells, so that the problem of cartilage injury in the early stage of osteoarthritis is solved, and an innovative strategy and material selection are provided for tissue engineering.

The invention belongs to the technical field of biological medicine, and discloses application of miRNA Let-7a-5p in a medicine for promoting cartilage formation, the invention provides the effect of miRNA let-7a-5p in cartilage formation / generation for the first time, and experiments prove that miRNA let-7a-5p can remarkably promote cartilage cell proliferation, enhance the activity of cartilage cells and remarkably improve matrix secretion of cartilage cells, so that the effect of promoting cartilage formation / generation is achieved. The expression of type II collagen is increased (the type II collagen is the main component of the cartilage matrix). Meanwhile, the miRNA let-7a-5p can also regulate other genes (such as NBL1 and DUSP7 genes) to inhibit cartilage ossification, and is expected to be applied to cartilage injury repair and osteoarthritis treatment, cartilage regeneration promotion and cartilage injury repair.

The invention relates to the technical field of biological medicines, and particularly discloses application of adenosine monophosphate in preparation of a medicine for treating osteoarthritis. Cell tests show that the adenosine monophosphate solution has an inhibition effect on cartilage cell activity; animal model tests show that the adenosine monophosphate normal saline solution can remarkably relieve the cartilage injury degree of osteoarthritis mice and relieve osteoarthritis, and a new way is provided for improvement of osteoarthritis conditions. The chemical structural formula of adenosine monophosphate is shown in the specification.

The invention belongs to the technical field of biological medicines, and particularly relates to application of xanthosine monophosphate in preparation of a medicine for treating osteoarthritis. The molecular formula of the xanthosine monophosphate is C10H13N4O9P, and the structure of the xanthosine monophosphate is shown in the specification. Research results show that the flavin monophosphate solution has an inhibition effect on cartilage cell activity; animal model tests show that the flavin monophosphate normal saline solution can remarkably relieve the cartilage injury degree of osteoarthritis mice and relieve osteoarthritis, and a new way is provided for improvement of osteoarthritis conditions. .

The invention discloses a mesenchymal stem cell compound preparation for treating cartilage injury, and belongs to the technical field of cell biology. The composite preparation consists of human umbilical cord mesenchymal stem cells (hUC-MSC) subjected to specific pretreatment and a pharmaceutical carrier. The pretreatment method sequentially comprises the following steps: carrying out low-oxygen pretreatment on the hUC-MSC; then, carrying out pretreatment on a ferroptosis inhibitor Ferrostatin-1 under a low-oxygen condition; and finally, carrying out icariin pretreatment under a normal oxygen condition. The unique triple pretreatment strategy generates a remarkable synergistic effect, and can greatly improve the survival rate and the function maintenance capability of the hUC-MSC in the cartilage injury inflammatory microenvironment. Experimental results prove that the preparation can significantly inhibit collagen degradation and promote cartilage matrix regeneration, and the treatment efficiency of stem cell transplantation and the repair effect of cartilage injury are effectively improved.

This invention provides a stress-triggered selective targeting hydrogel microsphere, its preparation method, and its application, belonging to the field of biomedical technology. The core of this invention is the "stress-triggered charge reversal" mechanism. It utilizes the interaction between weakly cross-linked disulfide bonds broken under stress and "charge reversal" messenger molecules to achieve "charge reversal" of nanoparticles under stress. A dual-network hydrogel microsphere system is prepared using microfluidic technology to achieve bearing lubrication and stress relaxation within the joint cavity. Stress-triggered charge reversal is achieved at stress-overloaded cartilage lesions, imparting a positive charge to the drug-loaded nanoparticles within the hydrogel microspheres. This significantly enhances the ability of the drug-loaded nanoparticles to penetrate deep into the cartilage matrix and target chondrocytes for drug release under the guidance of the charge. This enables the selective and targeted release of appropriate drug concentrations to deep-seated chondrocytes in pathological microenvironments induced by different stress intensities, achieving highly effective and low-toxicity treatment of osteoarthritis.

The present application relates to a kind of polypeptide for cartilage repair and its composition and application, belong to bone injury repair technical field.The polypeptide is polypeptide PG155, and the experimental results of the present application show that it can repair cartilage damage caused by osteoarthritis to some extent.Further research shows that the special exosome (PG155-Exo) produced by polypeptide PG155 pretreatment bone marrow mesenchymal stem cells combined with polypeptide PG155, can significantly enhance the therapeutic effect, and show obvious synergistic effect.This synergistic effect makes the cartilage repair effect significantly better than single therapy, and provides more efficient treatment scheme for osteoarthritis patients.

This invention relates to a method for constructing a humanized pig model of type II collagen and its application, belonging to the field of gene modification technology. The invention first provides a CRISPR-Cas9 system for targeted editing of the porcine type II collagen α1 gene. Using CRISPR / Cas9-mediated homologous recombination, the porcine COL2A1 gene is orally replaced with the human COL2A1 gene. Then, using somatic cell cloning technology, a humanized pig model of type II collagen is constructed, allowing the pig to produce cartilage tissue more similar to that of humans, reducing post-transplant immune rejection. This is expected to fundamentally solve the problem of human cartilage tissue shortage and has significant implications for the treatment of articular cartilage damage, providing a better and more suitable transplant material for the treatment of articular cartilage damage.

The invention relates to application of platelets modified by matrix metalloproteinase 13 in prevention and treatment of arthritis. Specifically, the invention provides a platelet complex, which is characterized by comprising platelet cells, a chemical linker element and a specific protein, wherein the platelet cells are linked to the specific protein through the chemical linker element, and the specific protein comprises an antibody targeting a cartilage injury specific expression molecule. The platelet complex (platelet at MMP13) disclosed by the invention is combined with MMP13 released by active targeting OA cartilage injury lesion sites, and cartilage repair and regeneration can be promoted, so that the effect in drugs for preventing and / or treating OA can be achieved.

The invention relates to regenerated cartilage based on hydrostatic pressure stimulation and a preparation method and application thereof. The method comprises the following steps: inoculating cells on a porous hydrogel scaffold, placing the porous hydrogel scaffold in a culture medium, performing pressure stimulation by adopting a hydrostatic pressure bioreactor, and performing in-vitro culture for a period of time to obtain the regenerated cartilage based on hydrostatic pressure stimulation. Key mechanical sensitive channels TRPV4 and PIEZO1 are up-regulated through hydrostatic pressure stimulation, a Ca < 2 + > dependent TRPV4 / PIEZO1-Ca < 2 + >-SOX9 mechanical transduction pathway is activated, cartilage formation is promoted, and hypertrophy and ossification are inhibited. Through verification, the bioreactor system is combined with the porous hydrogel scaffold, has good biocompatibility, controllable pore structure and mechanical property, and is suitable for cartilage cell culture. After in-vitro pre-culture for 4 weeks, the cartilage-like construct subjected to HP pretreatment shows a long-term stable regeneration effect in an animal model (such as a goat), and especially in an in-situ auricle microenvironment, inflammation and heterotopic ossification can be effectively inhibited, and an efficient in-vitro cartilage engineering strategy is provided for repairing of various cartilage injuries.

The present invention relates to a biomaterial comprising: —a membrane wound patch (a), made of a nanofibrous polymeric scaffold, —a hydrogel (b) including autologous or allogenic bone marrow-derived mesenchymal stem cells, and —a bone wound patch (c) being a nanofibrous scaffold made of polymers, wherein said scaffold has a surface coated with an interrupted coating made of multilayered droplets, said multilayered droplets being droplets composed of at least one layer pair consisting of a layer of polyanions and a layer of polycations, and wherein the bone wound patch (c) further comprises a bone growth factor; wherein the hydrogel (b) is included between the membrane wound patch (a) and the bone wound patch (c).

The invention discloses an antler plate oligopeptide as well as a preparation method and application thereof, and relates to the technical field of bioactive peptides. The preparation method comprises the following steps: extracting an antler plate protein crude extract by taking antler plates as a raw material, preparing short peptides with antler plate molecular weight of less than 3k through pepsin and trypsin hydrolysis, and performing Sephadex G-15 gel chromatography to obtain the antler plate short peptides. The prepared antler plate oligopeptide is low in molecular weight and high in hydrophobicity, easily penetrates through a biological membrane barrier to reach an in-vivo effective part, fully plays the biological activity effect of small molecule peptide, can effectively promote C28 / I2 cartilage cell proliferation, can increase the expression of osteoarthritis related phenotype protein Collagen II in an in-vitro model, can reduce the expression of MMP3, MMP13 and ADAMTS-5, and can be used for treating osteoarthritis. Meanwhile, cartilage matrix degradation in an osteoarthritis rat model is inhibited, cartilage injury is improved, and inflammatory response induced by osteoarthritis is reduced.

Hydrolyzed collagen compositions are described which are used for treatment of impaired cartilage or to prevent damage to healthy cartilage. These compositions function across multiple cartilage components and structures. Oral, topical, or cartilage-site delivery of the compositions to cartilage may be via liquid, gel. powder, or sheet form. Additionally, the hydrolyzed collagen composition may be applied as a coating on a medical device.

A lubricin-localized cartilage-like tissue and a method for producing the same, wherein, when in an arbitrary cross section passing a first center of mass of a cartilage-like tissue derived from pluripotent stem cells or a center-of-mass region, which is a portion inside a concentric sphere being centered at the first center of mass and having a diameter of [first major diameter×0.2], the expression level of lubricin per unit area contained in a central region, which is a portion inside a concentric circle being centered at a second center of mass that is the center of mass of the cross section and having a diameter of [major diameter of cross section (second major diameter)×(0.4 to 0.9)] is referred to as the central lubricin level and the expression level of lubricin per unit area contained in the non-central region is referred to as the non-central lubricin level.

The utility model relates to a pusher for cartilage injury repair under an arthroscope, which belongs to the technical field of medical instruments and comprises a push rod and an outer sleeve, the push rod is movably arranged in the outer sleeve and is in interference fit with the outer sleeve, and the tail end of the outer sleeve is connected with a handle convenient to hold; an inclined groove is formed in the head end of the outer sleeve, the outer sleeve comprises a semi-groove pipe, and the semi-groove pipe is used for guiding other auxiliary instruments to conduct an operation; according to the pusher for the cartilage injury repair operation under the arthroscope, repair materials are pushed to a designated injury position through cooperation of the push rod and the outer sleeve, the head end of the outer sleeve is designed to be of an inclined groove structure so that laying and operation of the cartilage materials in the operation can be facilitated, and the semi-groove pipe is arranged to guide other auxiliary instruments to conduct the operation; according to the utility model, the surgical operation is more convenient and quicker, the repair material can be effectively prevented from being damaged, and the surgical efficiency is improved.

A method for enhancing cartilage regeneration following its wounding is provided which includes identifying a mammal as experiencing an injury to its cartilage applying to it a composition including graphene oxide (GO) and hyaluronic acid (HA) that are covalently linked, XAV939, and water. The composition of the method may also include a surfactant, such as PEG. The composition can be topically administered to a subject to treat cartilage wounding of the subject.

The present invention relates to a complex for promoting cartilage differentiation comprising stem cells or a culture thereof and a cartilage cell-free crush, a method for fabricating cartilage by using the complex, cartilage fabricated via the method, a pharmaceutical composition comprising the complex for preventing or treating arthropathy, a method for preventing or treating arthropathy using the composition, a composition for inducing cartilage cell differentiation comprising the prepared cartilage cells, and a method for fabricating cartilage using the prepared cartilage cells. The complex for promoting cartilage differentiation comprising the stem cells or the culture thereof and the cartilage cell-free crush provided in the present invention is not only differentiated into cartilage cells without side effects even in vivo, but also promotes differentiation of inherent stem cells in vivo into cartilage cells via a paracrine effect exhibited in the differentiated cartilage cells to effectively regenerate the damaged cartilage, and thus the complex may be widely used for treatment of arthropathy involving the cartilage damage.