Bionic multilayer collagen support for cartilage repair and preparation method therefor

A collagen scaffold and cartilage repair technology, applied in tissue regeneration, bone implants, medical science, etc., can solve problems such as low effectiveness, low induction of biomaterials, and high risk of immune rejection

Active Publication Date: 2020-06-09
GUANGDONG GENERAL HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Therefore, in order to be able to simultaneously solve the deficiencies in the current practice of cartilage repair, that is, low inductivity of biomaterials, high risk of immune rejection, low effectiveness and high cost of

Method used

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  • Bionic multilayer collagen support for cartilage repair and preparation method therefor
  • Bionic multilayer collagen support for cartilage repair and preparation method therefor
  • Bionic multilayer collagen support for cartilage repair and preparation method therefor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] After pulverizing the stripped tendon, add 10 times the volume of 4M guanidine hydrochloride prepared by pH=7.5 and 0.05M Tris-HCl;

[0081] After suspension, pulverize the homogenate, stir at 4°C for 24 hours, centrifuge at 12000g for 20 minutes, and separate the supernatant and precipitate;

[0082] The precipitate was fully washed with Tris-HCl buffer and 0.5mM acetic acid, and then added acetic acid overnight to remove proteoglycans;

[0083] Dissolve the tendon with pepsin-containing glacial acetic acid solution, continue to process for a period of time until the mixture becomes a colorless, transparent, viscous liquid, centrifuge at 4°C for 20 minutes under 5000g centrifugal force, and collect the supernatant as the crude tendon collagen stock solution;

[0084] Add the sodium chloride solution to it, and keep stirring until white flocculent precipitates are precipitated from the solution, and continue to add the sodium chloride solution until the precipitate is n...

Embodiment 2

[0091] After pulverizing the stripped tendon, add 10 times the volume of 4M guanidine hydrochloride prepared by pH=7.5 and 0.05M Tris-HCl;

[0092] After suspension, pulverize the homogenate, stir at 4°C for 24 hours, centrifuge at 12000g for 20 minutes, and separate the supernatant and precipitate;

[0093] The precipitate was fully washed with Tris-HCl buffer and 0.5mM acetic acid, and then added acetic acid overnight to remove proteoglycans;

[0094] Dissolve the tendon with pepsin-containing glacial acetic acid solution, continue to treat for a period of time until the mixture becomes a colorless, transparent, viscous liquid, centrifuge at 4°C for 20 minutes at 5000g centrifugal force, and collect the supernatant as the crude tendon collagen stock solution;

[0095] Add the sodium chloride solution into it, keep stirring until white flocculent precipitates are precipitated from the solution, and continue to add the sodium chloride solution until the precipitate no longer p...

Embodiment 3

[0102] After pulverizing the stripped tendon, add 10 times the volume of 4M guanidine hydrochloride prepared by pH=7.5 and 0.05M Tris-HCl;

[0103] After suspension, pulverize the homogenate, stir at 4°C for 24 hours, centrifuge at 12000g for 20 minutes, and separate the supernatant and precipitate;

[0104] The precipitate was fully washed with Tris-HCl buffer and 0.5mM acetic acid, and then added acetic acid overnight to remove proteoglycans;

[0105] Dissolve the tendon with pepsin-containing glacial acetic acid solution, continue to treat for a period of time until the mixture becomes a colorless, transparent, viscous liquid, centrifuge at 4°C for 20 minutes at 5000g centrifugal force, and collect the supernatant as the crude tendon collagen stock solution;

[0106]Add the sodium chloride solution to it, and keep stirring until white flocculent precipitates are precipitated from the solution, and continue to add the sodium chloride solution until the precipitate is no long...

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Abstract

The invention relates to medical materials, particularly relates to the field of cartilage injury regeneration and mainly discloses a bionic multilayer collagen support for cartilage repair and a preparation method therefor. The support product disclosed by the invention has a multilayer three-dimensional space structure; through lowering of immunogenicity of materials and multilayer stacked design of a dense layer, a porous layer and a barrier layer, collecting of in-vivo cells is promoted, and exogenous-cell-free biomaterial induced in-vivo cartilage regeneration is achieved; and a regeneration policy is optimized, so that the support is applicable to the treatment and regeneration of the cartilage repair.

Description

technical field [0001] The invention relates to a medical material, in particular to the field of cartilage injury regeneration, and mainly discloses a bionic multi-layer collagen scaffold for cartilage repair and a preparation method thereof. Background technique [0002] Osteochondral defects in joints caused by trauma or bone disease are common in clinical practice and seriously affect the quality of life of patients. It has become one of the main causes of physical disability. Knee disease can occur in people of all ages. Cartilage defects in the knee can result from trauma, sprains, overuse of the knee, muscle weakness, or general wear and tear. Due to the special tissue structure and biological characteristics of articular cartilage, although many methods are known to treat articular cartilage defects, including but not limited to: subchondral bone drilling, electrical stimulation, laser, drug and cell injection, gene therapy, etc., However, none of the above methods...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/24A61L27/56A61L27/12A61L27/50A61F2/28A61F2/30
CPCA61L27/24A61L27/56A61L27/12A61L27/50A61F2/28A61F2/30756A61F2/30942A61F2/2846A61L2430/06A61F2002/2835A61F2310/00293
Inventor 郑秋坚邓展涛马元琛李梦远郑铭豪
Owner GUANGDONG GENERAL HOSPITAL
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