32results about How to "Promote recruitment" patented technology

The present invention relates to an engineered immune cell expressing (i) a cell surface molecule that specifically recognizes a ligand, (ii) an exogenous interleukin, and (iii) an exogenous Flt3L, XCL2, and / or XCL1. The invention also provides the use of the engineered immune cell in the treatment of cancer, infection or autoimmune disease. Compared with the traditional engineered immune cell, the engineered immune cell has remarkably improved tumor killing activity.

The invention discloses a coaxial electrostatic spinning fibrous scaffold, belonging to the field of biological tissue engineering. The scaffold comprises an aliphatic polyester shell coupled with bone marrow derived mesenchymal stem cell (BMSC) affinity peptides and a polyvinylpyrrolidone core loading a recombinant human transforming growth factor (TGF)-beta1, not only is safe and non-toxic and has excellent biocompatibility but also can collect more BMSCs and promote chondrogenic differentiation of BMSCs, and then more cartilage tissues are obtained, so that cartilages are effectively and quickly repaired. The invention also discloses a preparation method of the coaxial electrostatic spinning fibrous scaffold. The preparation method comprises the steps of carrying out coaxial electrostatic spinning by using an aliphatic polyester shell solution and a polyvinylpyrrolidone core solution containing the recombinant human TGF-beta1 in the windless environment to prepare a fibrous scaffold; coupling the BMSC affinity peptides on the surface of the fibrous scaffold, thus obtaining a nanoscale coaxial electrostatic spinning fibrous scaffold. The method is simple, is easy to control and has relatively strong practicability.

The invention discloses a calcium phosphate bone cement with a hollow through structure, a preparation method and an application thereof, belongs to the technical field of calcium phosphate bone cement, wherein the calcium phosphate bone cement with a hollow through structure comprises a solid phase and a solidifying liquid, the solid phase comprises calcium phosphate powder and a fiber mixture, the fiber mixture is an absorbable fiber and a fibrin fiber, and the diameter of the absorbable fiber is 100-500mum, the length is 0.5 mm-1.5 mm, preferably, that diameter of the absorbable fiber is 200-400mum. The prepared calcium phosphate bone cement can form three-dimensional connected macropores, promote cell adhesion and proliferation, and improve the mechanical strength of the bone cement.

This invention discloses immunopotentiating agents which stimulate an immune response. These agents are categorized into single agents that act directly, adjuvants added concurrently with the agents, or heteroconjugates. Heteroconjugate agents elicit or enhance a cellular or humoral immune response which may be specific for an epitope contained within an amino acid sequence. Enhanced hematopoieses by bone marrow stem cell recruitment was also a result of administering some of these agents. Examples of immunopotentiating agents include monoclonal antibodies and proteins derived from microorganisms (e.g., enterotoxins) which activate T cells. One method of treatment disclosed uses only the immunopotentiating agent to stimulate the immune system. Another uses adjuvants in combination with the agent. A third method employs heteroconjugates. Heteroconjugates comprise: (a) an immunopotentiating protein which is characterized as having an ability to stimulate T cells; and (b) a second protein having an amino acid sequence which includes an epitope against which a cellular or humoral response is desired. This invention also relates to a method of preparing the heteroconjugate, and to a method of stimulating the immune system in vivo in a novel way. One route of stimulation is to activate T cells, in some instances, specific subsets of T cells, by administering heteroconjugates containing an immunopotentiating protein and a second protein, to mammals. For this method of treatment, the second protein in the heteroconjugate is derived from abnormal or diseased tissue, or from an infectious agent; alternatively, the second protein is produced synthetically by standard methods of molecular biology. Sources of the second protein include tumors, viruses, bacteria, fungi, protozoal or metozoal parasites. Monoclonal antibodies or T cells prepared from mammals whose immune systems have responded to administration of the heteroconjugate may be produced and administered to induce passive immunity. A method of preparing a hybridoma which secretes said monoclonal antibodies and use of these monoclonal antibodies and T cells, are also disclosed. This invention is also directed to a vaccine comprising the heteroconjugate.

The invention relates to an injectable bone induction repair material and a preparation method thereof, and belongs to the technical field of biomedical materials. The preparation method specifically comprises the following steps of: S1, performing mineralization treatment on biological bones to obtain mineral substances from the biological bones, and crushing the mineral substances from the biological bones to obtain composite inorganic mineralized particles; S2, performing oscillation adsorption on active factors and the composite inorganic mineralized particles, so that the active factors are stably adsorbed on the surfaces of the composite inorganic mineralized particles through electrostatic attraction to obtain adsorbed composite inorganic mineralized particles; and S3, uniformly mixing the adsorbed composite inorganic mineralized particles with a gel aqueous solution to obtain the bone induction repair material. The bone induction repair material prepared by the preparation method is easy to shape and can be used through injection; and the bone induction repair material has ultrahigh crystallinity and crystallization purity, and pores are communicated with each other, so that cells can be combined with the active factors to promote cell recruitment.

The invention discloses an HMBG (High Mobility Group Box) 1 modified bone tissue engineering bracket material and a preparation method thereof. The HMBG1 modified bone tissue engineering bracket material is prepared by crosslinking a signal molecule HMGB1 to a PLLA (Poly L Lactic Acid) nanofiber bracket by means of heparin (2) and a crosslinking molecule Di-NH2-PEG (Polyethylene Glycol) by using the PLLA nanofiber bracket manufactured by an electrostatic spinning technology as a matrix. According to the invention, various functions of recruitment, infiltration and mobility of cells are realized by the signal molecule, so that the material has good biocompatibility, and can be used to compose stem cells and further can be directly applied to bone tissue repair. The material is simple and accessible in process and good in repeatability.

The invention relates to medical materials, particularly relates to the field of cartilage injury regeneration and mainly discloses a bionic multilayer collagen support for cartilage repair and a preparation method therefor. The support product disclosed by the invention has a multilayer three-dimensional space structure; through lowering of immunogenicity of materials and multilayer stacked design of a dense layer, a porous layer and a barrier layer, collecting of in-vivo cells is promoted, and exogenous-cell-free biomaterial induced in-vivo cartilage regeneration is achieved; and a regeneration policy is optimized, so that the support is applicable to the treatment and regeneration of the cartilage repair.

The invention discloses a coaxial electrostatic spinning fibrous scaffold, belonging to the field of biological tissue engineering. The scaffold comprises an aliphatic polyester shell coupled with bone marrow derived mesenchymal stem cell (BMSC) affinity peptides and a polyvinylpyrrolidone core loading a recombinant human transforming growth factor (TGF)-beta1, not only is safe and non-toxic and has excellent biocompatibility but also can collect more BMSCs and promote chondrogenic differentiation of BMSCs, and then more cartilage tissues are obtained, so that cartilages are effectively and quickly repaired. The invention also discloses a preparation method of the coaxial electrostatic spinning fibrous scaffold. The preparation method comprises the steps of carrying out coaxial electrostatic spinning by using an aliphatic polyester shell solution and a polyvinylpyrrolidone core solution containing the recombinant human TGF-beta1 in the windless environment to prepare a fibrous scaffold; coupling the BMSC affinity peptides on the surface of the fibrous scaffold, thus obtaining a nanoscale coaxial electrostatic spinning fibrous scaffold. The method is simple, is easy to control and has relatively strong practicability.

Disclosed are methods and compositions useful in the recruitment, activation and initiation of proliferation of immune cells. The instant invention relates to the discovery that specific insect cells and insect cell compositions exhibit adjuvant properties. The added benefit of this system is that the insect cells utilized may be transformed with an expression system and thus proteins may be introduced into the composition through direct protein expression by the cells. The claimed compositions and methods are particularly relevant in anti-tumor and cancer therapy. Of specific interest is the ability of the compositions of the invention to elicit a response that controls not only the primary tumor but also any metastatic cells or metastatic tumors which subsequently arise.

The invention provides a preparation method and a product of an earthworm breeding companion soil remediation agent. The soil remediation agent is used for soil remediation by taking earthworm biomass improvement as a target, a rotten earthworm glandula accessoria ion stress matrix is used as a substrate, an antibacterial earthworm fiber skeleton is filled, an annulata earthworm cocoon synthesis promoting agent benefiting earthworm cocoon, an earthworm symbiotic bacterium agent and an artificial earthworm cocoon barrier are matched, so that the remediation agent is formed. The biomass of the soil earthworm cocoons and earthworms can be increased by 4.40 and 2.9.

The invention discloses a selenium-doped hydroxyapatite nano-enhanced collagen GBR membrane and a preparation method thereof, and the preparation method comprises the following steps: (1) preparing selenium-doped hydroxyapatite (Se-HAP), and synthesizing Se / (Se + P) into a Se-HAP nano-structure through a solvothermal method; (2) adding animal skin collagen into an acetic acid solution, stirring to prepare a collagen solution, mechanically stirring until the collagen is completely dissolved, and uniformly dispersing the collagen in an interval low-temperature ultrasonic manner; the preparation method comprises the following steps: preparing a collagen solution, adding Se-HAP nanowire powder into an aqueous solution, fully stirring to prepare Se-HAP slurry, uniformly mixing the collagen solution and the Se-HAP slurry to obtain a collagen / Se-HAP mixed solution, performing ultrasonic treatment on the collagen / Se-HAP mixed solution, concentrating the collagen solution into a collagen solution, performing centrifugal treatment on the collagen solution, and cooling the collagen solution to 0 DEG C or below on a freezing device to obtain the collagen / Se-HAP nanowire composite material. The selenium-doped hydroxyapatite nanowire enhanced collagen GBR membrane can be applied to medical rehabilitation for guiding bone regeneration, and has excellent properties of antibacterial property and promoting osteogenic differentiation and regeneration.

The invention relates to bioactive polypeptide and an application thereof, which belong to the technical field of polypeptide exploitation. An amino acid sequence of the bioactive polypeptide is shownas SEQ ID NO.1. The polypeptide can promote migration and homing of injured tissues of a plurality of stem cells and differentiation of adult cells. According to the invention, angiogenesis can be promoted in myocardial ischemia and lower limb ischemia diseases, blood supply is improved, and tissue repair regeneration can be accelerated; the bioactive polypeptide can be used for skin injury, canpromote tissue repair of the skin, induce the migration, proliferation and differentiation of the stem cells of the skin, and can promote the angiogenesis and function recovery of an injured part.

The present invention provides a system of publicly raising funds for activities that assists operators who want to fund activity funds in publicly recruiting funds and is risk-free for the investors.A server connectable to an application provider terminal and an investor terminal in order to publicly recruit activity funds for providing a service for an application program comprises: a means toregister on a database project information, a means to allow said investor terminal to browse said project information, a means to store the money invested on said project by the investors in an investment money pool account, a means to issue to the application software provider activity tokens in a number corresponding to the total amount of investment money, a means to transfer from said investment money pool account to a predetermined payee money to be used for the business to provide the application software, and a means to deduct the activity tokens memorized in the database in a number corresponding to the amount of the transferred money.

The invention discloses an artificial blood vessel and a preparation method thereof. The preparation method comprises the following steps: preparing a catheter substrate, wherein the surface of the catheter substrate is provided with charges; preparing a first drug solution and a second drug solution, wherein the charges carried by the first drug solution and the second drug solution are different in electrical property, and the charges carried by the first drug solution and the charges carried by the catheter substrate are different in electrical property; and sequentially and alternately soaking the catheter substrate in the first drug solution and the second drug solution, so that a drug coating is formed on the surface of the catheter substrate. The charges carried by the surface of the catheter substrate are fully utilized, so that layer-by-layer self-assembly of two-component drugs with different charges on the surface of the substrate is realized; and after the blood vessel is implanted, due to sequential release of two drugs with different functions, the drug functions are exerted in different time periods, and the synergistic effect of the two drugs is achieved, so that improvement of long-term smoothness of the artificial blood vessel is ensured, and regeneration and reconstruction of the blood vessel are promoted.

The invention relates to a preparation method of a nanofiber gel composite matrix for constructing skin tissue. The main gel solution in the porcine fibrin adhesive is injected into the main gel freeze-dried powder to obtain a fibrin solution; Ester and silk fibroin are added to a container containing formic acid to obtain a PCL / SF spinning solution; the PCL / SF spinning solution is used as the inner phase, and the fibrin solution is used as the outer phase to perform spinning to obtain PCL coated with fibrin / SF core-shell nanofibers composed of nanofabric; spray the freeze-dried powder catalyst solution on the obtained nanofabric to form a gel, and obtain a nanofiber gel composite matrix for constructing skin tissue. This method is easy to operate and versatile, and can achieve large-area skin regeneration in abdominal full-thickness skin defects.

The present invention relates to an engineered immune cell expressing (i) cell surface molecules that specifically recognize ligands, (ii) exogenous interleukins, and (iii) exogenous Flt3L, XCL2 and / or XCL1. The present invention also provides the use of the engineered immune cell in the treatment of cancer, infection or autoimmune disease. Compared with traditional engineered immune cells, the engineered immune cells of the present invention have significantly improved tumor killing activity.

The invention discloses a nerve conduit loaded with gradient density active particles and a preparation method. The nerve conduit comprises an inner layer and an outer layer, wherein the outer layer is an electrostatic spinning fiber layer; and the inner layer is an electrostatic spraying particle layer of bioactive particles with gradient density. The deposition density of the bioactive particles on the surface of the inner layer structure is in gradient distribution. By regulating and controlling the topological structure of the spinning fiber and combining with the gradient active particle structure, the functions of early-stage release, time-space controllable release and the like of the active particles are realized, and the difficulties existing in the current research can be solved, so that various induction signals for promoting peripheral nerve repair are efficiently combined; the active particles are regulated and guided to be released from the near end to the far end in the peripheral nerve regeneration process, axons are promoted to stretch from the near end to the far end, peripheral nerve repair is effectively accelerated, and the repair effect is improved.

The invention discloses an enterprise personnel evaluation management system and an evaluation method thereof, the evaluation management system comprises an application layer, a processing layer and amanagement layer, the application layer is an application end of an evaluation module in the evaluation management system, for example, test questions are displayed on a computer end, and personnel to be tested answer questions; the processing layer is used for receiving the personnel answering information returned by the application layer and verifying the personnel answering information so as to obtain a test result and evaluation of personnel; and the management layer is used for managing the evaluation management system. In the present invention, creating a cloud sharing module, the question bank of each enterprise is shared with evaluation personnel and industry standard specifications in the industry; therefore, the latest examination, evaluation and answer can be obtained in time,the industry is unified, standardized and perfected, the industry is more standardized, meanwhile, the evaluation results of the evaluation personnel are shared, other companies can directly check theevaluation results of the personnel when the evaluation personnel jump out of the groove, the skill level of the personnel can be visually known, and talent recruitment is facilitated.

The invention relates to a distributed hearing experiment method, and belongs to the technical field of psychoacoustics, environmental acoustics and sound quality. The distributed hearing experiment method comprises the steps of: establishing a relation curve between the sound quality evaluation result and each acoustic parameter to serve targeted regulation and control of the sound quality; and determining volume of a sound sample output by a playback system adopted by an experiment by referring to a sound quality evaluation value of the sound sample in the experiment; and finally, acquiring the sound sample actually heard at the cochlea in the experiment accurately. According to the distributed hearing experiment method, recruitment of the experimental subject is facilitated, and the problem of how to enable the time domain and frequency domain characteristics of the sound sample actually heard at the cochlea to be the same as those of the original sound sample (there is only volume difference between the two sound samples) is solved.

The invention discloses an HMBG (High Mobility Group Box) 1 modified bone tissue engineering bracket material and a preparation method thereof. The HMBG1 modified bone tissue engineering bracket material is prepared by crosslinking a signal molecule HMGB1 to a PLLA (Poly L Lactic Acid) nanofiber bracket by means of heparin (2) and a crosslinking molecule Di-NH2-PEG (Polyethylene Glycol) by using the PLLA nanofiber bracket manufactured by an electrostatic spinning technology as a matrix. According to the invention, various functions of recruitment, infiltration and mobility of cells are realized by the signal molecule, so that the material has good biocompatibility, and can be used to compose stem cells and further can be directly applied to bone tissue repair. The material is simple and accessible in process and good in repeatability.

The invention relates to a novel brain-targeted drug delivery system, and particularly discloses a brain-targeted drug delivery system with ferroptosis and immune activation cascade amplification effects, in the presence of H2O2, siPD-L1 is connected to the surfaces of Fe3O4 nanoparticles through disulfide bonds, and a microglial cell membrane is used as a shell to construct siPD-L1-loaded bionic nanoparticles. The drug delivery system not only can silence the expression of PD-L1 in drug-resistant brain glioma cells and improve the immune microenvironment of the drug-resistant brain glioma, but also can promote the occurrence of ferroptosis, and finally forms a virtuous circle of mutual promotion between ferroptosis and immunotherapy to synergistically treat the drug-resistant brain glioma.

The invention relates to a continuous array gradient nanofiber bionic stent and a preparation method thereof. The method comprises the following steps of: performing electrostatic spinning to obtain an array gradient nanofiber membrane, and performing sterilization and crosslinking treatment to obtain the continuous array gradient nanofiber bionic stent, wherein a receiving device adopted by electrostatic spinning comprises a printed circuit board and an annular electrode; the printed circuit board comprises an insulating substrate and a plurality of bonding pads; the bonding pads are distributed in a way that one bonding pad is distributed in the center of the insulating substrate, and the rest bonding pads form a plurality of annular array bonding pad groups which are distributed circularly; the bottom of the annular electrode is fixed on the insulating substrate; seen from the top of the annular electrode, a circle formed by the inner wall of the annular electrode and circles formed by the annular array bonding pad groups are concentric circles; the circle centers of the concentric circles are the center of the insulating substrate. The stent has a continuous array gradient fiber structure, so that a medicament can be released at the edge and the center of a wound surface at different concentrations.

The present invention relates to a fibrous wound repair scaffold loaded with phase change material particles, and a preparation method and application thereof. The invention uses aliphatic polyester, natural macromolecules and phase change materials as main raw materials, adds antibacterial drugs, active substance particles and growth factors, and prepares a multi-layered scaffold with biological activity through electrospinning, electrostatic spraying and the like. The fiber wound repair scaffold provided by the invention adds different kinds of antibacterial drugs to achieve multiple antibacterial effects; contains biologically active substance particles with increased deposition density gradient to promote cell recruitment and migration; contains phase change material particles that promote repair growth factors, To modulate the behavior of different cells by photothermally-triggered controllable delivery of growth factors. The scaffold material provided by the invention has excellent biocompatibility and degradability, can mediate cell migration, proliferation, differentiation and paracrine effect, and can speed up wound repair and improve wound healing effect.

The invention discloses a bone defect repair tissue engineering stent and a preparation method thereof, and belongs to the field of bone repair. The bone defect repair tissue engineering stent comprises a metal stent body, a BMSC affinity peptide and a BMP-2 mimic peptide, wherein the BMSC affinity peptide and the BMP-2 mimic peptide are grafted on the metal stent body. According to the bone defect repair tissue engineering stent and preparation method thereof of the present invention, the BMSC affinity peptide and the BMP-2 mimic peptide are synchronously bonded to the surface of the metal stent body, such that sufficient BMSC cells can be collected on an interface between a host bone bed and a joint prosthesis surface, the BMSC cells can gain the good osteogenic differentiation ability, and the osseointegration strength between the host bone bed and the joint prosthesis can be significantly improved; the BMSC affinity peptide and the BMP-2 mimic peptide are coupled to the surface of the metal stent body in a chemical grafting mode, so that the preparation method of the engineering stent is simple, and large-scale popularization and application of the engineering stent are facilitated.

The invention belongs to the field of biomedical materials and tissue engineering, and in particular relates to a bionic skin dressing having a 3D micropattern structure and a preparation method thereof. The biomimetic skin dressing is obtained by curing a polymer composite gel into a composite gel film having a 3D micropattern structure, and then using calcium alginate for high hydrophilic surface treatment. The polymer composite gel is prepared by compounding RGD-peptide-containing methacrylate gelatin and methacrylate hyaluronic acid in a mass ratio of 1:3 to 3:1. The bionic skin dressing having the 3D micropattern structure has a straight stripe groove or a mesh stripe groove array, RGD peptide in the groove array can promote cell adhesion, and the calcium alginate in a convex array structure region can effectively inhibit cell growth, so that cells can selectively grow on the surface of the bionic skin dressing to form the cell micropattern, and the 3D micropattern structure can promote the ability of stem cells to differentiate into fibroblasts and angioblast in vivo and in vitro.