30results about How to "Promote recruitment" patented technology

The invention discloses a coaxial electrostatic spinning fibrous scaffold, belonging to the field of biological tissue engineering. The scaffold comprises an aliphatic polyester shell coupled with bone marrow derived mesenchymal stem cell (BMSC) affinity peptides and a polyvinylpyrrolidone core loading a recombinant human transforming growth factor (TGF)-beta1, not only is safe and non-toxic and has excellent biocompatibility but also can collect more BMSCs and promote chondrogenic differentiation of BMSCs, and then more cartilage tissues are obtained, so that cartilages are effectively and quickly repaired. The invention also discloses a preparation method of the coaxial electrostatic spinning fibrous scaffold. The preparation method comprises the steps of carrying out coaxial electrostatic spinning by using an aliphatic polyester shell solution and a polyvinylpyrrolidone core solution containing the recombinant human TGF-beta1 in the windless environment to prepare a fibrous scaffold; coupling the BMSC affinity peptides on the surface of the fibrous scaffold, thus obtaining a nanoscale coaxial electrostatic spinning fibrous scaffold. The method is simple, is easy to control and has relatively strong practicability.

The invention discloses a calcium phosphate bone cement with a hollow through structure, a preparation method and an application thereof, belongs to the technical field of calcium phosphate bone cement, wherein the calcium phosphate bone cement with a hollow through structure comprises a solid phase and a solidifying liquid, the solid phase comprises calcium phosphate powder and a fiber mixture, the fiber mixture is an absorbable fiber and a fibrin fiber, and the diameter of the absorbable fiber is 100-500mum, the length is 0.5 mm-1.5 mm, preferably, that diameter of the absorbable fiber is 200-400mum. The prepared calcium phosphate bone cement can form three-dimensional connected macropores, promote cell adhesion and proliferation, and improve the mechanical strength of the bone cement.

The invention relates to an injectable bone induction repair material and a preparation method thereof, and belongs to the technical field of biomedical materials. The preparation method specifically comprises the following steps of: S1, performing mineralization treatment on biological bones to obtain mineral substances from the biological bones, and crushing the mineral substances from the biological bones to obtain composite inorganic mineralized particles; S2, performing oscillation adsorption on active factors and the composite inorganic mineralized particles, so that the active factors are stably adsorbed on the surfaces of the composite inorganic mineralized particles through electrostatic attraction to obtain adsorbed composite inorganic mineralized particles; and S3, uniformly mixing the adsorbed composite inorganic mineralized particles with a gel aqueous solution to obtain the bone induction repair material. The bone induction repair material prepared by the preparation method is easy to shape and can be used through injection; and the bone induction repair material has ultrahigh crystallinity and crystallization purity, and pores are communicated with each other, so that cells can be combined with the active factors to promote cell recruitment.

The invention discloses an HMBG (High Mobility Group Box) 1 modified bone tissue engineering bracket material and a preparation method thereof. The HMBG1 modified bone tissue engineering bracket material is prepared by crosslinking a signal molecule HMGB1 to a PLLA (Poly L Lactic Acid) nanofiber bracket by means of heparin (2) and a crosslinking molecule Di-NH2-PEG (Polyethylene Glycol) by using the PLLA nanofiber bracket manufactured by an electrostatic spinning technology as a matrix. According to the invention, various functions of recruitment, infiltration and mobility of cells are realized by the signal molecule, so that the material has good biocompatibility, and can be used to compose stem cells and further can be directly applied to bone tissue repair. The material is simple and accessible in process and good in repeatability.

The invention relates to medical materials, particularly relates to the field of cartilage injury regeneration and mainly discloses a bionic multilayer collagen support for cartilage repair and a preparation method therefor. The support product disclosed by the invention has a multilayer three-dimensional space structure; through lowering of immunogenicity of materials and multilayer stacked design of a dense layer, a porous layer and a barrier layer, collecting of in-vivo cells is promoted, and exogenous-cell-free biomaterial induced in-vivo cartilage regeneration is achieved; and a regeneration policy is optimized, so that the support is applicable to the treatment and regeneration of the cartilage repair.

The invention discloses a selenium-doped hydroxyapatite nano-enhanced collagen GBR membrane and a preparation method thereof, and the preparation method comprises the following steps: (1) preparing selenium-doped hydroxyapatite (Se-HAP), and synthesizing Se/(Se + P) into a Se-HAP nano-structure through a solvothermal method; (2) adding animal skin collagen into an acetic acid solution, stirring to prepare a collagen solution, mechanically stirring until the collagen is completely dissolved, and uniformly dispersing the collagen in an interval low-temperature ultrasonic manner; the preparation method comprises the following steps: preparing a collagen solution, adding Se-HAP nanowire powder into an aqueous solution, fully stirring to prepare Se-HAP slurry, uniformly mixing the collagen solution and the Se-HAP slurry to obtain a collagen/Se-HAP mixed solution, performing ultrasonic treatment on the collagen/Se-HAP mixed solution, concentrating the collagen solution into a collagen solution, performing centrifugal treatment on the collagen solution, and cooling the collagen solution to 0 DEG C or below on a freezing device to obtain the collagen/Se-HAP nanowire composite material. The selenium-doped hydroxyapatite nanowire enhanced collagen GBR membrane can be applied to medical rehabilitation for guiding bone regeneration, and has excellent properties of antibacterial property and promoting osteogenic differentiation and regeneration.

The invention relates to bioactive polypeptide and an application thereof, which belong to the technical field of polypeptide exploitation. An amino acid sequence of the bioactive polypeptide is shownas SEQ ID NO.1. The polypeptide can promote migration and homing of injured tissues of a plurality of stem cells and differentiation of adult cells. According to the invention, angiogenesis can be promoted in myocardial ischemia and lower limb ischemia diseases, blood supply is improved, and tissue repair regeneration can be accelerated; the bioactive polypeptide can be used for skin injury, canpromote tissue repair of the skin, induce the migration, proliferation and differentiation of the stem cells of the skin, and can promote the angiogenesis and function recovery of an injured part.

The present invention provides a system of publicly raising funds for activities that assists operators who want to fund activity funds in publicly recruiting funds and is risk-free for the investors.A server connectable to an application provider terminal and an investor terminal in order to publicly recruit activity funds for providing a service for an application program comprises: a means toregister on a database project information, a means to allow said investor terminal to browse said project information, a means to store the money invested on said project by the investors in an investment money pool account, a means to issue to the application software provider activity tokens in a number corresponding to the total amount of investment money, a means to transfer from said investment money pool account to a predetermined payee money to be used for the business to provide the application software, and a means to deduct the activity tokens memorized in the database in a number corresponding to the amount of the transferred money.

The invention discloses an artificial blood vessel and a preparation method thereof. The preparation method comprises the following steps: preparing a catheter substrate, wherein the surface of the catheter substrate is provided with charges; preparing a first drug solution and a second drug solution, wherein the charges carried by the first drug solution and the second drug solution are different in electrical property, and the charges carried by the first drug solution and the charges carried by the catheter substrate are different in electrical property; and sequentially and alternately soaking the catheter substrate in the first drug solution and the second drug solution, so that a drug coating is formed on the surface of the catheter substrate. The charges carried by the surface of the catheter substrate are fully utilized, so that layer-by-layer self-assembly of two-component drugs with different charges on the surface of the substrate is realized; and after the blood vessel is implanted, due to sequential release of two drugs with different functions, the drug functions are exerted in different time periods, and the synergistic effect of the two drugs is achieved, so that improvement of long-term smoothness of the artificial blood vessel is ensured, and regeneration and reconstruction of the blood vessel are promoted.

The invention relates to a novel brain-targeted drug delivery system, and particularly discloses a brain-targeted drug delivery system with ferroptosis and immune activation cascade amplification effects, in the presence of H2O2, siPD-L1 is connected to the surfaces of Fe3O4 nanoparticles through disulfide bonds, and a microglial cell membrane is used as a shell to construct siPD-L1-loaded bionic nanoparticles. The drug delivery system not only can silence the expression of PD-L1 in drug-resistant brain glioma cells and improve the immune microenvironment of the drug-resistant brain glioma, but also can promote the occurrence of ferroptosis, and finally forms a virtuous circle of mutual promotion between ferroptosis and immunotherapy to synergistically treat the drug-resistant brain glioma.

The invention relates to a continuous array gradient nanofiber bionic stent and a preparation method thereof. The method comprises the following steps of: performing electrostatic spinning to obtain an array gradient nanofiber membrane, and performing sterilization and crosslinking treatment to obtain the continuous array gradient nanofiber bionic stent, wherein a receiving device adopted by electrostatic spinning comprises a printed circuit board and an annular electrode; the printed circuit board comprises an insulating substrate and a plurality of bonding pads; the bonding pads are distributed in a way that one bonding pad is distributed in the center of the insulating substrate, and the rest bonding pads form a plurality of annular array bonding pad groups which are distributed circularly; the bottom of the annular electrode is fixed on the insulating substrate; seen from the top of the annular electrode, a circle formed by the inner wall of the annular electrode and circles formed by the annular array bonding pad groups are concentric circles; the circle centers of the concentric circles are the center of the insulating substrate. The stent has a continuous array gradient fiber structure, so that a medicament can be released at the edge and the center of a wound surface at different concentrations.

The invention provides an application of an HVCN1 antibody in preparation of a medicine for treating nerve injury or neurodegenerative diseases, the HVCN1 antibody can enhance the migration ability of microglia or macrophage so as to promote the microglia or macrophage to collect and swallow myelin fragments in an injury area, the myelin fragments are removed, and the treatment effect of the HVCN1 antibody on nerve injury or neurodegenerative diseases is improved. A new method is provided for removing the myelin sheath fragments, and the effect of treating myelin sheath fragment accumulation diseases is fundamentally achieved; and the medicine is good in taking safety, low in cost and high in patient compliance.