Bioactive polypeptide and application thereof

A technology of biologically active polypeptides and biologically active components, applied to biologically active polypeptides and their application fields, can solve the problems of high cost and low cell survival rate, and achieve the effects of improving blood supply, inducing migration, proliferation and differentiation, and promoting repair.

Inactive Publication Date: 2018-08-24
徐清波
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Tissue ischemia can be effectively treated by injecting stem cells directly into the ischemic injury site, but it is expensive and has low cell survival rate, which may be related to the damage of cells in poor locality (ischemia hypoxia, inflammation, lack of matrix protection) environment related

Method used

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  • Bioactive polypeptide and application thereof
  • Bioactive polypeptide and application thereof

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preparation example Construction

[0024] In the present invention, there is no special limitation on the method for obtaining the biologically active polypeptide, and the polypeptide synthesis method well known to those skilled in the art can be used for artificial synthesis. Specifically, the solid-phase synthesis method of the biologically active polypeptide of the present invention comprises the following steps:

[0025] (1) The C-terminal of the first amino acid is grafted on the carboxyl resin, and the loading rate is 0.6mmol / g; 20% of piperidine is dissolved in N,N'-dimethylformamide (DMF), and the amino group is removed The Fmoc protecting group; In the present invention, the carboxyl resin includes Wang resin or 2-chlorotriphenyl chloride resin;

[0026] (2) Under the combined action of the condensing agent O-benzotriazole-tetramethyluronium hexafluorophosphate (HBTU) and the catalyst N,N-diisopropylethylamine (DIPEA), the carboxyl group of the next amino acid Combine with free amino groups; repeat th...

Embodiment 1

[0098] Solid Phase Synthesis of {L-Met}{L-His}{L-Ser}{L-Pro}G{L-Ala}{L-Asn} Peptides:

[0099] Peptides are synthesized by a well-established solid-phase synthesis (SPPS) procedure using 2-chlorotrityl chloride resin and amino acids with side chains protected by tert-buty groups and amino groups protected by Fmoc. The steps are:

[0100] (1) The C-terminus of the first amino acid was grafted on 2-chlorotrityl chloride resin, and the loading rate was 0.6mmol / g. 20% piperidine is dissolved in N, N'-dimethylformamide (DMF), and the Fmoc protecting group of the amino group is removed;

[0101] (2) Under the joint action of condensing agent O-benzotriazole-tetramethyluronium hexafluorophosphate (HBTU) and catalyst N, N-diisopropylethylamine (DIPEA), the carboxyl group of the next amino acid Combine with free amino groups; repeat the above steps, the polypeptide chain will continue to grow;

[0102] (3) Use a certain volume of DMF to repeatedly rinse the resin with the polypeptid...

Embodiment 2

[0106] Solid Phase Synthesis of {D-Met}{L-His}{L-Ser}{L-Pro}G{D-Ala}{D-Asn} Peptides:

[0107] Peptides are synthesized by a well-established solid-phase synthesis (SPPS) procedure using 2-chlorotrityl chloride resin and amino acids with side chains protected by tert-buty groups and amino groups protected by Fmoc. The steps are:

[0108] (1) The C-terminus of the first amino acid was grafted on 2-chlorotrityl chloride resin, and the loading rate was 0.6mmol / g. 20% piperidine was dissolved in N, N'-dimethylformamide (DMF) for removing the Fmoc protecting group of the amino group;

[0109] (2) Under the joint action of condensing agent O-benzotriazole-tetramethyluronium hexafluorophosphate (HBTU) and catalyst N, N-diisopropylethylamine (DIPEA), the carboxyl group of the next amino acid Combine with free amino groups; repeat the above steps, the polypeptide chain will continue to grow;

[0110] (3) Use a certain volume of DMF to repeatedly rinse the resin with the polypeptide (5...

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Abstract

The invention relates to bioactive polypeptide and an application thereof, which belong to the technical field of polypeptide exploitation. An amino acid sequence of the bioactive polypeptide is shownas SEQ ID NO.1. The polypeptide can promote migration and homing of injured tissues of a plurality of stem cells and differentiation of adult cells. According to the invention, angiogenesis can be promoted in myocardial ischemia and lower limb ischemia diseases, blood supply is improved, and tissue repair regeneration can be accelerated; the bioactive polypeptide can be used for skin injury, canpromote tissue repair of the skin, induce the migration, proliferation and differentiation of the stem cells of the skin, and can promote the angiogenesis and function recovery of an injured part.

Description

technical field [0001] The invention relates to the technical field of polypeptide development, in particular to a biologically active polypeptide and its application. Background technique [0002] The morbidity and mortality of cardiovascular diseases top the list of various diseases. According to the "China Cardiovascular Disease Report 2017" compiled by the National Center for Cardiovascular Diseases, the number of patients with cardiovascular diseases in my country is about 290 million, and cardiovascular disease deaths account for Residents' disease deaths accounted for more than 40%. [0003] Blood is a medium that provides important substances such as oxygen and nutrients for tissues and cells. Insufficient blood supply due to vascular damage, vascular blockage, etc. often leads to hypoxia and nutrient deficiency in tissues and cells, which in turn induces tissue and cell damage. of necrosis. Cardiovascular diseases caused by tissue ischemia include lower extremity i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06A61L26/00A61K9/06A61K38/08A61P17/02A61P9/10A61P13/12A61P7/04A61P29/00A61P31/02
CPCC07K7/06A61K9/06A61K38/00A61L26/0023A61L26/0028A61L26/0033A61L26/0066A61L26/008A61L26/0085A61L2300/252A61L2300/404A61L2300/41A61L2300/412A61L2300/418A61L2300/422A61P7/04A61P9/10A61P13/12A61P17/02A61P29/00A61P31/02
Inventor 徐清波
Owner 徐清波
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