4results about How to "Induced differentiation" patented technology

The present application relates to Foskolin induced differentiation treatment of hepatocellular carcinoma. Specifically, the present application relates to a method and use of Forskolin for inducing differentiation of human malignant hepatocellular carcinoma, thereby being applied to the treatment of malignant solid tumors. The research of the present application shows that after treating hepatocellular carcinoma cells with exogenous addition of Forskolin for a period of time, the hepatocellular carcinoma cells can be effectively induced to differentiate, thereby providing a new drug for tumor induced differentiation treatment. The purpose of the present application is to provide the use of Forskolin and its derivatives for anti-hepatoma drugs.

An object of the present invention is to provide a cerebral organoid derived from a human pluripotent stem cell, a cell aggregate including a cerebral cortical cell, and a method for producing any of them, each being useful for regenerative therapy. The method of the present invention for producing a cerebral organoid is a method for producing a cerebral organoid from a pluripotent stem cell in the absence of a sustentacular cell, and comprises: (1) a step of culturing the pluripotent stem cell in a culture solution, wherein the culture solution is substantially free of bFGF and provokes substantially no TGFβ signal; and (2) a step of inducing the cell obtained in step (1) to differentiate into a neural cell.

The present invention provides a composite artificial periosteum and a preparation method thereof, wherein the composite artificial periosteum comprises an umbilical cord layer and a gel layer; the umbilical cord layer is a sheet structure composed of umbilical cord tissue, wherein the umbilical cord tissue is formed from an umbilical cord from which vascular cells are mechanically removed; the gel layer is adhered to one side of the umbilical cord layer, wherein the gel layer is formed by a hydrogel network formed by a composite of chitosan and sodium alginate and loaded with bone repair mineral materials and freeze-dried, wherein the bone repair mineral materials are any one or two of silicon dioxide, tricalcium phosphate and bioactive glass. The artificial periosteum prepared by the present invention integrates barrier and repair, can play a protective role as a barrier membrane to prevent soft tissue invasion, can effectively guide bone regeneration, provide an excellent microenvironment for the regeneration and structural reconstruction of bone components, can induce bone repair and regeneration, and can be degraded synchronously with the reconstruction of new tissue, greatly improving the healing rate of bone defects.

An object of the present invention is to provide a novel screening technique for selecting a useful pharmaceutical compound. The present invention to solve the above problem is a method for screening active ingredients of a medicine, including the following step A, step B and / or step C, and step D. [step A] a step of administering a candidate substance to an animal (excluding human); [step B] a step of observing undifferentiated cells in the animal that has undergone step A; [step C] a step of observing differentiated cells in the animal that has undergone step A; and [step D] a step of selecting, as the active ingredient, a candidate substance that improves the amount of undifferentiated cells, or a candidate substance that improves the amount of differentiated cells or a candidate substance that improves a function of a tissue composed of differentiated cells, as compared with a case where the candidate substance is not administered.