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Nerve conduit loaded with gradient density particles and preparation method

A nerve conduit and gradient density technology, applied in prosthesis, single-component polyester artificial filament, medical science, etc., can solve the problems of low biological activity and slow repair speed of peripheral nerve damage

Pending Publication Date: 2022-07-26
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The currently available clinically available nerve guide materials are mainly used to guide the degradation of peripheral nerve repair, but their biological activity is low and there are few active particles loaded, resulting in a slower repair speed of peripheral nerve damage.

Method used

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  • Nerve conduit loaded with gradient density particles and preparation method
  • Nerve conduit loaded with gradient density particles and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Step (1). Dissolve polycaprolactone in dichloromethane, stir magnetically at room temperature for 24h, to obtain solution D with a mass concentration of 15%;

[0064] Step (2). Electrospinning with solution D, using a roller with a rotating speed of 1000 rpm as a receiver, solution D advancing rate of 8.0 mL / h, voltage of 19 kV, receiving distance of 18 cm, spinning for 1000 min, to obtain uniaxial orientation The polycaprolactone nanofiber membrane;

[0065] Step (3). Dissolve laminin and fibronectin in an aqueous acetic acid solution, stir magnetically at room temperature for 24 hours, and mix thoroughly to obtain a shell solution E with a concentration of 15 mg / mL; take vascular endothelial growth factor and add it to deionized water, Fully stirring and dissolving to obtain the core layer solution F, the concentration is 10ug / mL, the dosage ratio of the polycaprolactone fiber membrane to the sum of the shell layer solution and the core-shell solution is: 1g:40mL; the...

Embodiment 2

[0069] Step (1). Dissolve polylactic acid in trifluoroethanol, and stir magnetically at room temperature for 20h to obtain solution D with a mass concentration of 5%;

[0070] Step (2). Electrospinning with solution D, using a roller with a rotational speed of 2000rpm as a receiver, solution D advancing rate of 5.0mL / h, voltage of 16kV, receiving distance of 13cm, spinning for 1000min, to obtain uniaxial orientation PLA nanofiber membrane;

[0071] Step (3). Dissolve laminin in trifluoroethanol, stir with magnetic force at room temperature for 24 hours, and mix thoroughly to obtain shell solution E with a concentration of 35 mg / mL; take vascular endothelial growth factor and nerve growth factor and add them to deionized In water, fully stir and dissolve to obtain core layer solution F, the concentration is 50ug / mL, the dosage ratio of polylactic acid fiber membrane to the sum of shell layer solution and core-shell solution is: 1g:30mL; the dosage ratio of shell layer solution ...

Embodiment 3

[0075] Step (1). Dissolve polylactic acid-glycolic acid copolymer in chloroform and N,N'-dimethylformamide, and stir magnetically at room temperature for 8h to obtain solution D with a mass concentration of 8%;

[0076] Step (2). Electrospinning with solution D, using a roller with a rotating speed of 1500 rpm as a receiver, solution D advancing rate of 4.0 mL / h, voltage of 26 kV, receiving distance of 28 cm, spinning for 840 min, to obtain uniaxial orientation The polylactic acid-glycolic acid copolymer nanofiber membrane;

[0077] Step (3). Dissolve laminin in a sterile aqueous solution, and mix thoroughly with magnetic stirring at room temperature for 24 hours to obtain shell solution E with a concentration of 30 mg / mL; take brain-derived nerve growth factor, human acidic fibroblast growth The factor is added into deionized water, and the core layer solution F is obtained by stirring and dissolving sufficiently. The dosage ratio of shell solution and core solution is 4:1; ...

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Abstract

The invention discloses a nerve conduit loaded with gradient density active particles and a preparation method. The nerve conduit comprises an inner layer and an outer layer, wherein the outer layer is an electrostatic spinning fiber layer; and the inner layer is an electrostatic spraying particle layer of bioactive particles with gradient density. The deposition density of the bioactive particles on the surface of the inner layer structure is in gradient distribution. By regulating and controlling the topological structure of the spinning fiber and combining with the gradient active particle structure, the functions of early-stage release, time-space controllable release and the like of the active particles are realized, and the difficulties existing in the current research can be solved, so that various induction signals for promoting peripheral nerve repair are efficiently combined; the active particles are regulated and guided to be released from the near end to the far end in the peripheral nerve regeneration process, axons are promoted to stretch from the near end to the far end, peripheral nerve repair is effectively accelerated, and the repair effect is improved.

Description

technical field [0001] The invention relates to the technical field of biological materials, and more particularly, to a nerve conduit loaded with gradient density active particles and a preparation method. Background technique [0002] Since there are currently 20 million cases of peripheral nerve injury in clinical practice in my country, and the number is increasing at a rate of about 2 million cases every year, the repair of peripheral nerve injury has become a major scientific issue in the field of nerve repair. The clinical "gold standard" is autograft; however, the limited sources of autografts, damage to the donor site, size mismatch, and secondary surgery limit its application. Therefore, it is urgent to develop artificial nerve conduits to repair nerve damage to solve the problem of lack of autologous grafts. [0003] At present, the clinically available nerve conduit materials mainly play the role of guiding the degradation of peripheral nerve after completion of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/22A61L27/24A61L27/50A61L27/54A61L27/58D01F6/62
CPCA61L27/18A61L27/227A61L27/225A61L27/24A61L27/222A61L27/50A61L27/54A61L27/58D01F6/62A61L2430/32A61L2300/412A61L2300/602A61L2300/414C08L67/04C08L89/00
Inventor 薛佳佳余逸玲张馨丹龚博文张立群
Owner BEIJING UNIV OF CHEM TECH
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