79 results about "Nerves regeneration" patented technology

The invention discloses an electrical stimulation type nerve regeneration method based on calcium signal regulation and control, and relates to the technical field of electrical stimulation type nerve regeneration, and the method comprises the following steps: based on a Schwann cell population calcium signal response feature set, identifying whether the calcium ion concentration is in a microliter stage which breaks through a lowest activation threshold and does not reach a stable state; judging whether the target nervous tissue is in a calcium signal activation critical state or not based on the behavior mode change of the Schwann cell population; based on the judgment result of the calcium signal activation critical state, an electrical stimulation adjustment control instruction is generated in real time in combination with the calcium ion concentration fluctuation trend and Schwann cell population response characteristics, and the calcium ion concentration stability is optimized and the activation state is maintained by adjusting the electrical stimulation intensity, the electrical stimulation pulse width, the electrical stimulation waveform type and the electrical stimulation application time sequence. According to the method, the problem that a calcium signal is difficult to recognize and dynamically optimize in a microliter stage is solved, and precise maintenance of Schwann cell activation and improvement of nerve regeneration efficiency are realized.

The invention discloses a structural bionic space-time coupled axon guidance-synchronous degradation type nerve implant as well as a preparation method and application thereof. According to the preparation method, after collagen and polyvinyl alcohol are compounded, freeze-drying is performed through a specific freeze-drying process to obtain the composite nerve implant with a bunchy multi-channel and porous structure, so that the defect that the degradation rate of a current application product is too high is overcome. The degradation rate required by the composite nerve implant is regulated and controlled according to the proportion of the composite materials, the composite materials which are rapidly degraded can be designed at the two ends of the composite nerve implant with the long length, and the composite materials which are slowly degraded can be designed at the middle part of the composite nerve implant with the long length, so that the mechanical performance required by nerve regeneration is supported. The obtained nerve implant not only is beneficial to preventing cicatrix tissues from entering, but also can be used as a topographic clue of nerve regeneration related cells to guide nerve regeneration, in addition, the degradation rate of the material is synchronous with the nerve regeneration process, and finally, the material is continuously degraded along with nerve regeneration and is replaced by new tissues.

A stimulation device includes an adaptor component. The adaptor component couples a percutaneous lead to the stimulation device. The stimulation device may apply a stimulation signal to target tissue via the adaptor. A surgeon may place the stimulation device in a container and the adaptor component may be disposed outside of the container. Methods describe prolonged stimulation of target tissue via a stimulation device. The prolonged stimulation may be applied during and after a surgical procedure.

The invention relates to a differentiated'inner core and outer sheath 'triboelectric stimulation bionic nerve graft and a preparation method thereof. The nerve graft is composed of an outer sheath layer and an inner core layer. Wherein an outer sheath layer is a calcium ion cross-linked waterborne polyurethane / sodium alginate double-network structure hollow conduit, and an inner core layer is oriented conductive polymer / silk fibroin nanofibers. The nerve graft provided by the invention is provided with the sheath part with an internal and external differential gradient hole structure and the bionic inner core similar to a natural nerve structure, when the nerve graft is implanted into a body, the inner core and the outer sheath show differential degradation speeds and hydrophilic-hydrophobic properties, and can generate electrical stimulation synchronously responding to physiological activities, so that regeneration after nerve injury is facilitated.

According to the injectable double-layer drug sustained-release hydrogel and the preparation method and application thereof, hydrogel swelling and degradation curve and mechanical property detection shows that extrusion secondary injury to optic nerves can be avoided due to the swelling ratio ratio of 14% of inner-layer hydrogel to 10% of outer-layer hydrogel; the hydrogel precursor solution prepared by the preparation method disclosed by the invention has proper mechanical strength and adhesive force so as to cope with an in-vivo environment, has good injectability, can control the gelling time within 2 minutes, perfectly conforms to a short time window of optic nerve decompression surgery, and has extremely high clinical practical value. The problems that the serious operation risk of cerebrospinal fluid leakage during optic nerve local administration and a traditional local administration carrier lacks in-situ stability and is prone to displacement are solved, the damaged microenvironment can be cooperatively regulated and controlled, and long-acting slow release of nerve regeneration is promoted; multiple functions such as operation safety, medicine long-acting slow release, biocompatibility and operation convenience are successfully integrated, and an unprecedented effective strategy is provided for solving the clinical problem of optic nerve injury.

The application belongs to the field of medical biological materials, and relates to a preparation method of a hierarchical nanofiber composite film for nerve repair and application thereof. The method comprises the following steps: preparing multilayer nanofibers with different internal stresses through continuous multiple electrostatic spinning units, stacking the multilayer nanofibers with different internal stresses to form a composite nanofiber film with an internal stress gradient; chemically crosslinking the composite nanofiber film; performing adhesion molecule adhesion treatment on the upper surface of the composite nanofiber film; and preparing the hierarchical nanofiber composite film with an internal stress gradient and capable of self-deforming under the action of liquid to wrap nerves. The film provided by the application has excellent flexibility, biocompatibility and degradation performance, can efficiently and quickly adhere to the surface of damaged nerves, provides a good support microenvironment for nerve cell growth, promotes nerve regeneration and repair, and has a wide application prospect in the field of peripheral nerve injury repair.

The present application relates to the technical field of medical image processing, in particular to an image intelligent analysis system for corneal nerve fibers of dry eye patients, which comprises an image acquisition module, an image preprocessing module, a nerve fiber segmentation module, a nerve morphological analysis module based on differential geometry, a nerve regeneration monitoring module, a data management module and a clinical auxiliary decision module. The nerve morphological analysis module based on differential geometry is the core innovation, which contains a nerve fiber curvature analysis unit, a multi-scale differential invariant extraction unit and a nerve network topology structure quantification unit. The system introduces the theory of differential geometry, represents the nerve fiber through a parameterized curve and calculates the local curvature characteristics. A Gaussian scale space is constructed to extract multi-scale differential invariants. The graph structure representation of the nerve network is established to analyze the topological characteristics. The morphological characteristics of the corneal nerve fibers can be quantified comprehensively from the local curvature to the global topological structure, and the accurate detection of the slight changes of the nerve fibers is realized.

The invention relates to the technical field of biomedical materials, in particular to a high-conductivity peripheral nerve socketed pipe and a preparation method thereof. The nerve socketed pipe is a hollow cylindrical socketed pipe made of a material containing poly (octylene-citrate-co-octanol) POCO, and a regeneration promoting material is loaded in the hollow cylindrical socketed pipe; the regeneration promoting material comprises fetal calf serum and a DMEM cell culture medium. The POCO has the advantages of conductive activity, nerve regeneration induction, free radical scavenging ability, oxidation resistance, deformation resistance and the like, and has good degradability and biocompatibility, so that the nerve socketed pipe is prepared from poly (octylene-citrate-co-octanol), and the growth of axons is accelerated through electrical stimulation. Furthermore, at least one conductive monomer of pyrrole, aniline, p-phenylenediamine, o-methoxyaniline, thiophene, 3-hexylthiophene, 3-thiopheneacetic acid and 3, 4-ethylenedioxythiophene is adopted for modifying the POCO hollow cylindrical socketed pipe through in-situ oxidative polymerization, and the performance of the POCO hollow cylindrical socketed pipe serving as the neural restoration material is further improved.

The invention relates to a degradable nerve repair membrane and a preparation method thereof, and belongs to the technical field of nerve repair membranes, the degradable nerve repair membrane comprises a biological composite structure inner layer, a support outer layer and a bonding layer, and the biological composite structure inner layer and the support outer layer are bonded through the bonding layer. According to the invention, polypyrrole with excellent electrochemical activity, environmental stability, controllable conductivity and poor mechanical properties and collagen nanofibers with high biocompatibility and capable of forming a nano-scale fiber network are adopted to form a conductive-biological composite structure as an inner layer through electrostatic spinning, and the PLGA porous membrane is prepared through 3D printing. A PLGA porous membrane with parallel axial microchannels on the surface is used as an outer layer, so that the membrane body structure can effectively guide directional growth of axons while ensuring conductivity, accurate preparation can be realized according to needs, the degradation period is matched with the nerve regeneration rate, and the degradation time can be regulated and controlled through the proportion of a copolymer; the repairing effect is prevented from being influenced by too fast or too slow degradation.

The present application relates to a kind of water gel support prepared by self-assembled polypeptide and the use of support obtained therefrom, the preparation method includes: 1) self-assembled peptide sequence is linked nerve growth factor analog peptide by covalent bond to obtain bonded functional polypeptide;2) macrophage is separated and cultured, and is induced to be " alternative activation " anti-inflammatory M2 macrophage, obtain culture supernatant and carry out filtration, obtain filtered cell culture supernatant;3) filtered cell culture supernatant is mixed with bonded functional polypeptide, obtain mixed solution and adjust the concentration of the mixed solution, the bonded functional polypeptide self-assembles and forms water gel support.The present application uses M2 macrophage condition culture supernatant to construct regenerative microenvironment, combines polypeptide functional water gel support, realizes better overall interaction, and prepares the water gel support that can supplement and regulate nerve regeneration, the support can promote nerve cell regeneration behavior, provides new selection for tissue engineering biomaterials.

The invention provides a near-infrared chiral magnesium-based superparticle nerve photoconductive tube, a preparation method thereof and application of the near-infrared chiral magnesium-based superparticle nerve photoconductive tube in peripheral nerve regeneration, and belongs to the field of synthesis and application of medical stent materials. A chiral magnesium-based superparticle is provided; dispersing the chiral magnesium-based superparticles in an organic solvent, and adding polycaprolactone in batches to obtain a high-viscosity spinning solution; carrying out electrostatic spinning on the spinning solution, uniformly covering a roller with non-wrinkle tin foil paper, collecting the tin foil paper covered with spinning, cutting the tin foil paper into small pieces, and soaking the small pieces in absolute ethyl alcohol or a PBS (Phosphate Buffer Solution); the spinning film on the soaked small piece of tin foil paper is rolled into a chiral nerve conduit, after the chiral nerve conduit is rolled, the chiral nerve conduit is strung through a stainless steel rod and dried, plasticization of the conduit is achieved, and the near-infrared chiral magnesium-based superparticle nerve photoelectric conduit is obtained. The near-infrared chiral magnesium-based superparticle nerve photoconductive tube obtained by the invention has important significance on repair after sciatic nerve injury.

Disclosed in the present invention are a peripheral nerve catheter and a preparation method therefor. The peripheral nerve catheter comprises: an outer sheath, which is a hollow braided tube that has micron-sized pores and is non-wound; at least one inner core tube, which is placed in the outer sheath and is coaxially arranged with the outer sheath, each inner core tube being a hollow fiber tube that has micro / nano-sized pores and is non-wound; and filling gel, which is filled between the outer sheath and the inner core tube to fix the inner core tube. The bionic nerve catheter having a structure composed of the inner core and the outer sheath according to the present invention can achieve effective nerve regeneration, the inner core tube, which axially runs through the outer sheath, has a topological structure that is conducive to the adhesion and directional growth of nerve cells, and also has a micro / nano-sized pore structure that is conducive to the penetration of nutrients for growth and the formation of nerve connections and neurovascular network. In addition, the filling and curing of the filling gel effectively prevents the infection of damaged tissues, and is conducive to the penetration of nutrients and the transfer of cellular metabolic waste.

The invention provides a sequential slow-release hydrogen sulfide nerve repair conduit and a preparation method thereof, and belongs to the technical field of biological materials. The sequential slow-release hydrogen sulfide nerve repair conduit comprises degradable temperature-sensitive polyethylene glycol monomethyl ether-poly (cysteine-glycolic acid) hydrogel and a pH response hydrogen sulfide donor, wherein the degradable polymer conduit is filled with the degradable temperature-sensitive polyethylene glycol monomethyl ether-poly (cysteine-glycolic acid) hydrogel, and the pH response hydrogen sulfide donor is loaded on the hydrogel. In the application, the pH response donor can respond to an acidic microenvironment of degradation of polyethylene glycol monomethyl ether-poly (cysteine-glycolic acid) hydrogel, release hydrogen sulfide and relieve the acidic environment generated by degradation of the hydrogel at the same time, and form a dual supply mechanism with cysteine (endogenous hydrogen sulfide precursor) obtained by degradation of the hydrogel; nerve regeneration and angiogenesis are synergistically promoted, and oxidative stress is regulated; and the hydrogel is accelerated to degrade during excessive oxidative stress, so that hydrogen sulfide is released as required, and the core problems of insufficient blood supply, unbalanced acidic microenvironment and slow nerve regeneration are synchronously solved.

A nerve stimulation system comprising includes a housing, circuitry that generates an electrical stimulation signal, a microcontroller, one or more leads connected to the circuitry in the housing, and a return electrode electrically coupled to the circuitry, where the return electrode provides an electrical flow path for the stimulation signal from the one or more leads back to the housing. The system also includes a first control device and a second control device that cause the circuitry to generate the stimulation signal over a first channel or the second channel through the one or more leads. The system also includes one or more indicators fixed with the housing and electrically coupled to the microcontroller, where the one or more indicators generate visual indications, audio indications, or haptic indications that vary according to different status conditions of the circuitry on a per channel basis.

The present invention relates to the field of nerve repair technology, and discloses a hydrogen and magnesium ion pump catheter with a Janus structure for peripheral nerve defect repair and a preparation method thereof. The pump catheter is composed of a hydrophilic inner layer, a hydrophobic outer layer, and a reservoir middle layer. The hydrophilic inner layer and the hydrophobic outer layer are made of degradable polymer materials, and the reservoir middle layer is made of a mixture of one or more of polylactic acid, polyglycolide, or polylactic-glycolic acid and a magnesium-based material. The pump catheter has a Janus structure, the hydrophilic inner layer provides biophysical clues, improves the cell compatibility of the catheter, and promotes the adhesion and migration of nerve cells. The hydrophobic outer layer blocks the adhesion and invasion of unfavorable cells such as fibroblasts to the damaged area. The magnesium ion and hydrogen reservoir middle layer can continuously release hydrogen and magnesium ions, exerting biological effects, improving the nerve regeneration microenvironment, and improving the effect of the stent in repairing nerve damage.

An aligned nanofiber-based nerve-guidance conduit is provided which acts as a structural guide for assisting regeneration of a damaged nerve, and which provides an environment suitable for nerve regeneration through a porous channel. The nerve-guidance conduit is made of a material having biodegradability so that no additional removal surgery is required even after nerve regeneration is completed. In addition, the nerve-guidance conduit is composed of a piezoelectric material that generates electricity during deformation. Thus, a scheme for promoting the regenerative ability of nerve cells through electrical stimulation generated when the guidance conduit is deformed is proposed.

The application discloses an injectable double-layer drug sustained-release hydrogel and a preparation method and application thereof, and relates to the field of drug sustained-release hydrogels. The swelling and degradation curve and mechanical property detection of the hydrogel show that, when the swelling rate ratio of the inner layer hydrogel to the outer layer hydrogel is 14% to 10%, the secondary damage of the optic nerve caused by extrusion can be avoided; meanwhile, the hydrogel has suitable mechanical strength and adhesion to cope with the in-vivo environment. The hydrogel precursor solution has good injectability, and the gelation time can be controlled within 2 minutes, which perfectly matches the short time window of the optic nerve decompression surgery, and has high clinical practical value. The application solves the major surgical risk of cerebrospinal fluid leakage when the optic nerve is locally administered, and solves the problems of the traditional local drug carrier, such as lack of in-situ stability and easy displacement. The application can synergistically regulate the injury microenvironment and promote the long-acting sustained-release of nerve regeneration, successfully integrates multiple functions such as surgical safety, drug long-acting sustained-release, biocompatibility and operation convenience, and provides an unprecedented effective strategy for solving the clinical problem of the optic nerve injury.

The invention discloses an application of an MMP7 inhibitor MMP-7-IN-1 in preparation of a medicine for inhibiting formation of fiber scars and promoting tissue regeneration. Research results show that MMP-7-IN-1 can effectively inhibit fibroblast excessive proliferation and collagen deposition, improve local extracellular matrix microenvironment and provide favorable conditions for tissue repair, so that fiber scar formation is inhibited, and tissue repair and nerve regeneration are promoted; secondly, the inhibitor MMP-7-IN-1 has good structural stability, can be directly administered or be matched with other carriers for administration, is simple and convenient to operate and low in cost, and avoids the problem of toxic residues possibly generated in traditional chemical modification. Therefore, the invention provides clinical application of MMP-7-IN-1 in prevention and treatment of fiber scar related diseases and repair of various tissue damages, and the MMP-7-IN-1 shows remarkable practical value and popularization potential.

This invention relates to a method for preparing a directional nerve conduit with a dynamic microstructure, comprising the following steps: S1, preparation of a memory film material precursor solution; S2, preparation of a directional microstructure template; S3, preparation of a directional microstructure memory film; S4, programming and shaping treatment; S5, nerve conduit preparation: the directional microstructure memory film treated in step S4 is rolled and bonded to form a conduit. This invention also claims protection for the nerve conduit prepared by this method. Through the preparation method of this invention, the obtained nerve conduit can undergo specific changes in its directional microstructure by adjusting temperature, thus enabling more precise control of peripheral nerve cells, better acceleration of the physiological nerve regeneration process, and promotion of nerve defect healing and the creation of effector organ functionality; furthermore, the preparation method is simple, environmentally friendly, and easy for industrial production applications.

The application provides a ROS-regulated hydrogel loaded with acid koumiss-derived exosomes and a preparation method and application in preparation of burn drugs, and belongs to the technical field of burn drugs. The acid koumiss-derived exosomes (K-Exo) are wrapped by using silk fibroin peptides, and the obtained nano drug-loaded fibrous microcrystals can be used as a ROS-responsive nano drug-loaded sustained-release system of K-Exo. When the microenvironment of a burn site highly expresses ROS, the silk fibroin nano fiber microcrystals are dissolved, K-Exo is released, the ROS content and inflammatory cell infiltration at the damage site are effectively reduced, and the skin at the burn site is rapidly healed. The K-Exo is loaded into the GelMa hydrogel, and the obtained hydrogel shows broad-spectrum antioxidant and anti-inflammatory biological effects, as well as active regulation of the immune system, ROS-responsive controlled release, promotion of cell migration ability, nerve regeneration and blood vessel regeneration, so that the repair of large-area burn non-healing wounds can be improved.

The invention discloses a resveratrol and nerve growth factor loaded chitosan-polydopamine modified 3D printing biological material as well as a preparation method and application thereof in spinal cord injury repair, the structure and function of the biological material simulate a natural extracellular matrix (ECM) tissue structure to fill cavities in a spinal cord injury area, and tissue regeneration can be guided; meanwhile, the compound can be used as a carrier of medicines, cells or bioactive factors. According to the material, a three-dimensional porous PLA support with an accurate structure is printed through a fused deposition modeling (FDM) technology to serve as a carrier for cell compatibility and physical supporting, CS is loaded in the three-dimensional porous PLA support through a freeze drying technology, PDA carrying NGF is loaded on the surface of the three-dimensional porous PLA support, Res is loaded through the physical adsorption effect, and the multifunctional 3D biological composite material for spinal cord injury repair is prepared. Through the synergistic effect of anti-inflammatory, anti-oxidation, anti-apoptosis and other ways, a microenvironment beneficial to nerve regeneration is created together, and functional recovery after spinal cord injury is significantly promoted.

The application discloses a traditional Chinese medicine plaster for lumbar disc herniation and a preparation method thereof, and relates to the technical field of biological medicine. In the microneedle layer, macromolecular antler active components are loaded, and a liposome transition depot layer and an enzyme-responsive hydrogel controlled-release layer are constructed on the surface of the microneedle layer. Through the system integration of the three-layer structure, a complete treatment chain of puncture, transdermal penetration, transition depot, intelligent response and controlled release is constructed. The microneedle layer breaks through the physical barrier of the stratum corneum, directly delivers the antler active components and analgesic anti-inflammatory components to the dermis layer, and realizes rapid effect. The liposome transition depot layer provides secondary release and protection, maintains the drug concentration platform, and realizes continuous supply. The enzyme-responsive hydrogel controlled-release layer intelligently adjusts the drug release rate according to the pathological state of the lesion, and realizes on-demand drug release. The antler active components promote the repair of nucleus pulposus cells and nerve regeneration, and the CeO2@PDA nanoenzyme removes ROS to improve the microenvironment, realizing the treatment of both the symptoms and the root cause.

The invention provides a multifunctional drug delivery nerve conduit and a preparation method thereof, and belongs to the technical field of biological materials. Degradable microgel-based hydrogel filled in multifunctional drug delivery nerve conduit and multi-effect drug sustained-release nanoparticle methoxy polyethylene glycol-poly [methoxy polyethylene glycol-poly [methoxy polyethylene glycol-poly [methoxy polyethylene glycol-poly [methoxy polyethylene glycol-poly [methoxy polyethylene glycol-poly [methoxy polyethylene glycol-poly [methoxy polyethylene glycol-poly [methoxy polyethylene glycol-poly [methoxy polyethylene glycol-poly] loaded in degradable microgel-based hydrogel] The invention relates to a phenylboronic acid-diethylaminoethyl methacrylate-co-N-(2-nitroso mercaptan ethyl)-methacrylamide-co-methyl methacrylate) nano particle. The nano particle is prepared from phenylboronic acid and diethylaminoethyl methacrylate, according to the multifunctional drug delivery nerve conduit, multi-effect drug sustained-release nanoparticles and microgel-based hydrogel are combined, so that more cell adhesion sites are provided for nerve regeneration, and p-hydroxybenzyl alcohol is released through slow release of nitric oxide and active oxygen response; the problems that response release drugs cannot be treated in time and slow release drugs cannot be released as required are solved, dynamic adjustment of a nerve injury part regeneration microenvironment is achieved, and nerve regeneration and functional recovery are promoted.

The invention provides a dental pulp stem cell exosome-loaded acellular tissue engineering nerve conduit as well as a preparation method and application thereof, relates to the technical field of biomedical engineering and nerve regeneration medicine, and aims to solve the problems of poor mechanical property, low bionic degree, high degradation rate, insufficient biological activity and the like of an existing nerve conduit. The technical key point of the invention is as follows: the dental pulp stem cell exosome-loaded acellular tissue engineering nerve conduit is provided, and the nerve conduit is obtained by loading a nanofiber conduit with enhanced function of over-expressing let-7i-5p, the porous nanofiber catheter is a porous catheter which is obtained after a polyvinyl alcohol (PVA)-chitosan (CS)-graphene (G) electrostatic spinning membrane is subjected to pipe reeling and crosslinking, and the nucleotide sequence of let-7i-5p in DPSC-Exo is shown as SEQ NO.1. The invention further discloses a preparation method of the porous nanofiber catheter.

The invention discloses piezoelectric / conductive bifunctional composite nanoparticles of a barium titanate and graphene-like carbon layer core-shell structure as well as a preparation method and application of the piezoelectric / conductive bifunctional composite nanoparticles, and belongs to the technical field of biomedical materials. The piezoelectric / conductive bifunctional composite nanoparticle is composed of a barium titanate inner core and a graphene-like structure carbon shell coating the surface of the barium titanate inner core. According to the invention, a carbon shell with a graphene-like structure is prepared on the surface of the barium titanate nanoparticle through an innovative surface modification and compounding process, so that the compounding of piezoelectric property and conductive property on the same material is realized. According to the composite material, growth and differentiation of cells are directly promoted through the piezoelectric property, nerve regeneration is adjusted through the conductivity, and the tissue repair effect is further enhanced. The dual mechanism provides a synergistic effect for tissue repair and nerve regeneration, and has a wide application prospect.

The present invention relates to an aqueous ophthalmic composition comprising spirolactone, cyclodextrin and tromethamine, preferably for topical application to the human eye. The present inventors advantageously dissolve spirolactone in the HP-gamma cyclodextrin excipient at a concentration of 0.1%, which dose has been demonstrated to be effective in the treatment of ocular surface defects. They also have successfully demonstrated that the spirolactone eye drops are stable for up to 9 months at 4 DEG C and achieve its efficacy within at least 6 months, and the formulation is well tolerant after multiple daily instillations within 7 days. In addition, the spirolactone eye drops provided by the invention accelerate healing of corneal wounds of rats, reduce corneal edema and inflammation, enhance epithelial integrity and improve nerve regeneration, and prompt recovery of corneal homeostasis. Thus, the invention also relates to the use of an aqueous ophthalmic composition for the treatment of ocular diseases and disorders.

The invention belongs to the technical field of biology, and particularly relates to a corneal nerve bionic guiding support based on an electrospinning technology. The corneal nerve bionic guide stent provided by the invention is high in porosity and beneficial to cell attachment, migration and neurite extension, and during use, the stent structure can simulate the arrangement direction of corneal nerves, can effectively guide nerve axons to grow in a specific direction, and improves the nerve regeneration efficiency; according to the invention, the silk fibroin is compositely modified by adopting a plurality of growth factors such as NGF, NT-3, NT-4 / 5, NPY and VEGF, so that a multi-factor synergistic effect is realized, and related signal channels of nerve regeneration can be more comprehensively activated. After the polycaprolactone and the silk fibroin are compounded, complementary advantages are formed, the structural strength requirement of the cornea tissue engineering scaffold is met, good flexibility is kept, and the scaffold has good cell affinity and tissue integration capacity and is suitable for tissue engineering scaffolds.

The present application relates to the technical field of biological medicine, in particular to a functionalized targeted liposome and application thereof, comprising: lipids and a targeting ligand; wherein the targeting ligand can specifically bind to a TREM2 receptor. The liposome of the present application can specifically bind to the TREM2 receptor by means of the targeting ligand, realize precise targeting of cells containing the TREM2 receptor on the surface, ensure direct action on target cells, and further improve the precision of delivered substances. The liposome has targeting property, high stability, small side effects, and can be applied to the development of drug carriers, pharmaceutical compositions, etc., and has a wide application prospect. For example, the liposome of the present application is dynamically connected with a hydrogel molecule by a borate ester bond to prepare a hydrogel pharmaceutical composition containing the functionalized targeted liposome of the present application. The hydrogel pharmaceutical composition can release the liposome of the present application according to the change of pH and ROS in the surrounding environment in response to an inflammatory environment, and target cells containing the TREM2 receptor on the surface, i.e. microglial cells (having the TREM2 receptor on the surface), by virtue of the targeting ligand exposed on the surface, and regulate the polarization state of the cells, inhibit the polarization of the cells to the pro-inflammatory M1 type, and promote the polarization of the cells to the anti-inflammatory M2 type, thereby improving the local microenvironment of the spinal cord injury site and promoting nerve regeneration.

Disclosed are enhanced umbilical cord-derived adhesive stem cells, a preparation method therefor, and a use thereof. The enhanced umbilical cord-derived adhesive stem cells have an anti-inflammatory effect, a blood vessel regeneration effect, or a nerve regeneration effect, thereby being usable in a pharmaceutical composition or a cell therapeutic agent for treating or preventing various diseases.

Aspects of the claimed embodiments relate to compositions and methods for treating wounds and / or promoting wound healing and mammalian nerve regeneration of damaged nerves. Furthermore, the application relates to the use of a therapeutic amount of an endothelin-B receptor agonist. In particular, the present application relates to a method of treating dermal or skin wounds and damaged cranial or peripheral nerves using IRL-1620 (Sovastatin). In some embodiments, a composition may comprise an endothelin-B receptor agonist, which may comprise a peptide, such as Sovastatin (IRL-1620). Delivery systems comprising agents for topical applications, including gels, sponges, gauzes, and webs, are also described.