10 results about "Neurite" patented technology

The invention relates to the technical field of nerve cell differentiation, and discloses a culture medium for promoting neural differentiation of SH-SY5Y cells. The culture medium disclosed by the invention is prepared from the following components: tazarotene (tazarotene) and a basic culture medium. The culture medium can promote SH-SY5Y cells to present neuron-like differentiation within 5 days, significantly improves the length of neurite and the expression level of neuron markers, and is low in cytotoxicity. The invention provides an efficient, safe and standardized new tool for neural disease in-vitro model construction, drug screening and mechanism research.

A system and method are provided for determining a distribution of cellular populations in a patient. The method includes accessing neurite orientation dispersion and density imaging (NODDI) data acquired from the patient and performing a topological data analysis of the NODDI data to generate topological information. The method also includes correlating the topological information with cell population information and generating a report including the distribution of cellular populations in the patient based on the correlating of the topological information with cell population information.

This invention provides a pH-responsive immunomodulatory microneedle patch, its preparation method, and its application, belonging to the field of biomedical materials technology. This invention incorporates Zif-8 nanoparticles loaded with ChABC into GelMA hydrogel to prepare a pH-responsive immunomodulatory microneedle patch (Z@C-MN). As the patch gradually degrades, the system can continuously release Zn in a pH-responsive manner. 2+ Z@C-MN, along with ChABC, promotes in situ repair of cerebral fibrosis (SCI) after injury. Z@C-MN induces macrophage polarization towards a pro-regenerative phenotype, thereby downregulating the inflammatory response, increasing neurite length, and promoting the differentiation of neural stem cells into neurons. In vivo, Z@C-MN reduced the inflammatory microenvironment after injury and promoted angiogenesis in a rat SCI model. Furthermore, it activated axonal regeneration by inhibiting scar tissue formation and regulating CD4+. + T cell transformation supports neurogenesis.

The present disclosure relates generally to providing a cellular time-series imaging, modeling, and analysis platform, and more specifically to acquiring time-series image data and using various machine learning models to model and analyze subcellular particle movements and changes in cellular positional and morphological characteristics using unsupervised embedding generation. The platform can be applied to evaluate various cellular and subcellular processes by generating summary embeddings of time-series image data that enable analysis of dynamic cellular and subcellular processes over time (e.g., the movement of particles within a cell, neurites on developing neurons, etc.) for enhanced identification of differences between cell states (e.g., between sick and healthy cells) and generation of disease models which can be used to analyze the impact of various therapeutic interventions, among other improvements described throughout.

This invention discloses a GPU-based neuronal skeleton extraction method based on topology-simplified trunk growth, relating to the fields of neuroinformatics and 3D biomedical image processing. The method includes: threshold filtering and denoising of the input volume data, and retaining the largest connected component based on 3D connected component labeling to remove isolated noise; given the cell body center coordinates, determining the cell body region through spherical expansion and removing it from the foreground to highlight neural protrusions such as dendrites and axons; after obtaining the coarse skeleton, using GPU to perform topology simplification, and constructing a path scoring function combining path length and average normalized brightness, iteratively selecting and retaining the trunk path with the best score, enhancing trunk continuity and suppressing pseudo-branches caused by noise and breaks. This invention can achieve robust skeleton extraction in 3D data with noise interference and uneven brightness, and is suitable for applications such as neuronal morphology reconstruction and quantitative analysis.

The present invention relates to a polypeptide that induces ribbon synapse formation in the inner ear or increases the number of ribbon synapses in the inner ear, or improves the function of ribbon synapses. The present invention also relates to a polypeptide having the ability to promote neurite outgrowth or increase branch points of spiral ganglion neurons. A pharmaceutical composition comprising a polypeptide, a nucleic acid molecule encoding the polypeptide, a vector comprising the nucleic acid molecule, recombinant viral particles comprising the vector and a capsid protein, a cell comprising the vector, a cell transformed with the vector, or a combination thereof, of the present invention, prevents or ameliorates damage to or abnormality of the auditory system or the equilibrium system (vestibular system), and thus can be effectively used as a therapeutic agent for inner ear diseases.

This application discloses a neuromorphic organoid chip for high-throughput drug screening, belonging to the fields of microfluidic chips and drug screening technology. The chip includes a chip substrate and packaging module, a neuron culture module, an organoid culture module, a unidirectional trend connection module, a high-throughput drug delivery and distribution module, and a detection and sampling module. By integrating the neuron culture module, organoid culture module, unidirectional trend connection module with directional bias characteristics, and high-throughput drug delivery and distribution module on the same chip substrate, this invention achieves partitioned culture and directed connection of iPSC-induced neurons and three-dimensional tissues such as tumor organoids, supporting the construction of neuromorphic tumor models and high-throughput drug screening. This invention can guide neurites to preferentially extend towards the organoid side and inhibit the reverse migration of cells on the organoid side, offering advantages such as clear model boundaries, high throughput, and suitability for parallel drug testing and mechanism research.

A method of analyzing neurons includes: identifying a cell region by identifying a region of a neurite or a region of a cell body of a neuron on the basis of time-series images obtained by imaging the neuron in time series; detecting aggregates by imaging, in time series, first fluorescent protein tagged to specific protein expressed in the neuron and detecting presence or absence of aggregates of the specific protein aggregated in the region of the neurite or the region of the cell body identified in the identifying the cell region on the basis of luminance of the first fluorescent protein included in the time-series images; and performing an analysis by classifying the neuron into a plurality of groups on the basis of a detection result of the detecting the aggregates and analyzing a survival state of the neuron for each of the groups.

The invention discloses a dandelion root extract as well as a preparation method and application thereof in promoting regeneration of peripheral nerves, and the preparation method is characterized in that acetone, triethanolamine and sodium borohydride are taken as extracting agents, and non-toxic, efficient and cheap natural active ingredients are obtained from dandelion roots. The dandelion root extract provided by the invention is rich in flavone and sterol substances, can promote growth of nerve cells and neurite, promote mRNA expression increase of myelin sheath related factors, can also promote morphological structure regeneration and functional recovery of myelin sheath lamella of damaged nerves, and can be used for peripheral nerve injury repair and peripheral nerve regeneration promotion; the preparation method provided by the invention has the advantages of stable active components and high extraction rate.

This invention relates to TAFA proteins, fragments thereof, or variants thereof that have the ability to increase the length of neurites or branching points. Furthermore, this invention relates to pharmaceutical compositions containing polypeptides comprising the amino acid sequence of said TAFA protein, its fragments, or variants thereof; nucleic acid molecules encoding said polypeptides; vectors carrying said nucleic acid molecules; recombinant viral particles comprising said vector and capsid protein; cells comprising said vector; cells transformed with said vector; or therapeutic uses thereof. The compositions of this invention promote repair from damaged retina (e.g., cone cells of photoreceptors, etc.) or increase the length of neurites in neurons, and are therefore advantageous for the prevention or treatment of retinal neurological disorders and / or neuropathic pain.