The invention relates to a high-
porosity and high-
connectivity porous medium material for
intracellular delivery of therapeutic cells and a preparation method of the high-
porosity and high-
connectivity porous medium material. According to the microchannel, a porous
polymer medium is prepared in a hard pipeline through a hard template method, and the specific preparation method comprises the following steps: stirring and mixing a
thermosetting polymer and
alcohol-soluble particles, performing high-temperature curing, removing the
alcohol-soluble particles through absolute ethyl
alcohol, forming a porous structure, and constructing the microchannel for
intracellular delivery of animal cells. The channel with the
porous medium material has excellent
conductivity, and the
connectivity between pore diameters can reach 98%. And the pore size distribution is wide and is between 5 microns and 50 microns. On the premise of effectively enhancing the permeability of
cell membranes, good
cell activity is ensured, efficient and safe
intracellular delivery is realized, the highest
delivery efficiency can reach 91.2%, the highest
cell survival rate can reach 97.2%, and the net yield can reach 88.6%. Meanwhile, the method is simple in preparation process, convenient to operate and particularly suitable for the fields of
gene therapy,
drug development, cell research and the like.