33results about How to "Prevent invasion" patented technology

The application belongs to the technical field of biological medicine, and provides application of combined use of tocopherol and mulberroside C in preparation of a drug for treating liver cancer, which is combined use of tocopherol and mulberroside C in preparation of a drug for treating liver cancer. Through in-vitro cell experiments, the growth inhibition effect of tocopherol and mulberroside C on liver cancer cells is evaluated; the results show that tocopherol and mulberroside C can inhibit the growth activity of liver cancer cells to achieve the purpose of treating liver cancer.

This invention discloses an engineered probiotic expressing D-lactate dehydrogenase, its construction method, and its applications, belonging to the field of biomedical technology. The engineered probiotic of this invention is constructed by exogenously introducing the D-lactate dehydrogenase encoding gene into *Escherichia coli* Nissle 1917. This invention utilizes synthetic biology techniques to construct a "living therapeutic factory" capable of in-situ clearing specific metabolites, realizing a paradigm shift from "inhibiting proteins" to "clearing metabolites" in treatment. Through lipid coating technology, oral delivery and intestinal targeted colonization of the engineered bacteria are achieved, improving the precision and efficiency of treatment. Based on the probiotic EcN, it exhibits high biosafety. The treatment process is oral administration, non-invasive, and avoids the systemic toxicity of traditional chemotherapy.

The invention discloses a tumor detection marker and application thereof in medicine preparation, and belongs to the technical field of biomedicine. Clinical sample qRT-PCR (quantitative real-time polymerase chain reaction) shows that ENST00000412203.1 is obviously up-regulated in breast cancer tissues, the area (AUC) under the ROC curve reaches 0.9822, and the diagnosis efficiency is excellent. In addition, the invention further finds that the expression of the polypeptide in lymph node metastasis tissues is obviously improved, and metastasis specificity is displayed. Meanwhile, the expression of ENST00000412203.1 is knocked down through siRNA, it is found that proliferation, migration and invasion of breast cancer cells are remarkably inhibited, and therefore the siRNA can be used for preparing targeted therapy drugs for breast cancer and has important clinical application value.

The invention provides a bis-substituted phenyl acrylamide / ester compound as well as a preparation method and application thereof. The structural formula of the compound is shown as a formula (I) or a formula (II). The compound provided by the invention can be used as a covalent inhibitor of DHHC family proteins, and inhibits proliferation, migration and invasion of tumor cells and plays an anti-tumor role by inhibiting the activity of DHHC palmitoyltransferase and blocking the palmitoylation modification process of substrate proteins. Experimental results show that the bis-substituted phenylacrylamide / ester compound has remarkable anti-proliferative activity on various tumor cells, the inhibition effect of the compound is superior to that of a positive control drug 2-BP, and the bis-substituted phenylacrylamide / ester compound has the potential of being further developed into a new generation of efficient and low-toxicity anti-tumor drugs.

The utility model relates to a PE (Poly Ethylene) packaging film with moisture-proof and waterproof performance, which belongs to the technical field of packaging films and comprises a bottom layer used for contacting with a product, a contact bulge fixed at the bottom of the bottom layer, a buffer layer arranged above the bottom layer, an accommodating groove formed on the buffer layer through stamping, and a protective layer arranged above the buffer layer. A protective structure is embedded in the bottom of the protective layer, a top layer is arranged above the protective layer, the containing grooves are filled with expansion filler, the expansion filler is bentonite or a cement-based material, the number of the buffer layers is two, the number of the protective layers is two, the containing grooves in the buffer layers are arranged in a matrix mode, and the containing grooves in different layers are arranged in a staggered mode. According to the packaging film, friction force is increased through the bottom layer contact protrusions, sliding displacement of a product is avoided, and stability is enhanced; the buffer layer containing groove is filled with materials, water can be absorbed, holes can be blocked, impact force can be dispersed, and the damp-proof and waterproof performance is improved; the protective structure of the protective layer enhances the strength and toughness, so that the protection is more uniform, and the protective effect is enhanced.

The utility model discloses a kind of fireproof light power cable of rat and termite prevention, including conductor, the outside of the conductor is equipped with shielding layer, the outside of the shielding layer is equipped with insulating layer, the outside of the insulating layer is equipped with inner protective layer, the outside of the inner protective layer is equipped with physical protection layer, the outside of the physical protection layer is equipped with protective net layer, in the utility model, the power cable, by inner protective layer inner layer elastic memory resistance layer hardens and releases chlorpyrifos when receiving ≥5N bite pressure, outer layer honeycomb metal-ceramic composite layer improves bite resistance strength;Physical protection layer forms barrier with material hardness;Protective net layer can release medicament, and the defense capability of rat and termite bite is improved compared with traditional cable by multilayer protection;And insulating layer uses high-temperature resistant material to guarantee fireproof basis;Inner protective layer outer layer honeycomb metal-ceramic composite layer can form fireproof barrier when meeting fire.These structures cooperate and improve the fire resistance in fire situation compared with traditional cable.

This invention provides a monoclonal antibody specifically targeting fungal enolase 1, its preparation, and its application. The monoclonal antibody of this invention comprises the gene and amino acid sequences of the heavy chain variable region (VH) and the light chain variable region (VL). The monoclonal antibody or antibody fragment of this invention specifically targeting fungal enolase 1 can bind with high affinity to the fungal Eno1 protein and also bind to fungal cells, thereby inhibiting fungal adhesion, infection, and invasion of host cells. The monoclonal antibody provided by this invention has the characteristics of high specificity and good efficacy. When used alone, it can significantly treat invasive fungal infections. When used in combination with existing antifungal small molecule compound drugs, it can significantly enhance the efficacy of the small molecule compound drugs, achieving better therapeutic effects.

This invention discloses the application of small molecule compounds in the preparation of drugs for treating breast cancer. MEN 10207 was screened using molecular docking analysis, and its ability to significantly inhibit the proliferation, migration, and invasion of breast cancer cells was verified in MDA-MB-231 cells, indicating its potential as an inhibitor of breast cancer brain metastasis. Further studies found that MEN 10207 did not exhibit significant toxicity in zebrafish and showed good tolerability in mouse intravenous injection experiments. Treatment of zebrafish transplanted with breast cancer cells with MEN 10207 significantly reduced the survival and migration ability of breast cancer cells in zebrafish; treatment of a mouse model of breast cancer brain metastasis with MEN 10207 also significantly reduced the colonization of breast cancer cells in the mouse brain, confirming its in vivo activity against breast cancer brain metastasis.

The application discloses a kind of high flame-retardant temperature-resistant corrosion-resistant optical fiber composite cable and preparation method thereof, belong to cable manufacturing technical field, from inside to outside include optical fiber unit, conductor layer, insulating layer, shielding layer, anticorrosive layer and sheath layer in sequence.Optical fiber unit uses ceramicized silicone rubber buffer layer;Conductor layer is nickel-plated copper alloy stranded wire, and oxidation resistance is enhanced by nano titanium dioxide sol treatment;Insulating layer is three-layer co-extrusion structure;Shielding layer uses aluminum-plastic composite tape and plated tin copper wire braided composite structure;Anticorrosive layer is polytetrafluoroethylene film treated by plasma;Sheath layer uses two-component ceramicized silicone rubber, and it is flexible at room temperature vulcanization, and it is converted into ceramic body at high temperature.The application realizes excellent continuous use stability, strong corrosion resistance in salt spray environment and other performances through the synergistic design of multilayer structure, is suitable for high temperature, corrosion, fire and other harsh environments, significantly improves the safety and durability of composite cable.

This invention relates to the field of biomedical technology, and in particular to a method for preparing and applying a nanomedicine for treating deep endometriosis. The method involves mixing ferrous ammonium sulfate, trisodium citrate, and polyethyleneimine, then adding thioacetamide and triethanolamine via a solvothermal reaction to obtain two-dimensional iron sulfide nanosheets. Dopamine hydrochloride is then added and stirred at room temperature in anhydrous ethanol to obtain polydopamine-coated iron sulfide (FP). FP is then mixed with larotrectinib and anhydrous ethanol to obtain the nanomedicine FPL for treating deep endometriosis. The synthesis method of this invention is simple and highly operable; the product is stable and reproducible; FPL exhibits good biocompatibility and biodegradability. After entering the microenvironment of deep endometriosis lesions, it can responsively release larotrectinib, effectively inhibiting the Trk signaling pathway and significantly reducing the degree of nerve infiltration; simultaneously, the anti-inflammatory effect of the FP carrier alleviates the local inflammatory response, jointly inhibiting lesion progression.

The invention provides an outdoor power switch cabinet with high safety protection performance, which comprises a base and a cabinet body fixedly connected to the top wall of the base, and further comprises an anti-collision assembly and a power supply assembly, the anti-collision part comprises a supporting shaft rotationally connected to the inner wall of the base, a first gear and a second gear fixedly connected to the outer wall of the supporting shaft, and a buffering part slidably connected to the side wall of the base. The supporting part comprises a third gear, a fourth gear and a telescopic supporting part, the third gear and the fourth gear are fixedly connected to the outer wall of the supporting shaft, the telescopic supporting part is slidably connected to the side wall of the base, the telescopic supporting part is in linkage with the buffering part, and the reinforcing part is slidably connected to the inner wall of the reinforcing groove through a sliding groove; when the cabinet is impacted, the purpose of increasing the supporting range of the base is achieved, the overall stability is improved, meanwhile, when the supporting frame moves, the sliding blocks are pulled to move through the reinforcing rods, then the cabinet body is reinforced, the cabinet body is prevented from toppling over after being impacted, and the overall protection performance is improved.

The invention belongs to the technical field of compound fertilizers, and particularly relates to a compound fertilizer microbial fertilizer as well as a preparation method and application thereof. The preparation method of the compound microbial fertilizer comprises the following steps: respectively fermenting livestock slaughtering wastes, livestock wastes, waste melons and fruits and eggshells to obtain a solution a, a solution b, a solution c and a solution d; mixing the obtained solutions in proportion, adding molasses, bacillus subtilis, EM bacteria and trichoderma harzianum, fully mixing, and carrying out secondary fermentation to obtain a compound microorganism stock solution; and adding the nitrogen-phosphorus-potassium inorganic fertilizer and the compound microorganism stock solution, and fully stirring to obtain the fertilizer. The invention discloses a compound microbial fertilizer prepared by a preparation method, which is used for solving the problems of soil hardening, acidification and plant diseases and insect pests caused by chemical fertilizer application, soil quality improvement, harmful substance reduction and fertility improvement.

This invention discloses the pharmaceutical applications of a P2Y11-specific inhibitor and compositions containing it, belonging to the field of biomedical technology. This invention discloses for the first time the use of NF157 in the preparation of drugs related to the treatment of HD5-induced pathogen infection. In combating pathogen infection, NF157 can specifically inhibit the binding of P2Y11 to HD5, thereby eliminating the role of HD5 in regulating cytoskeleton rearrangement and promoting filopodia formation, thus becoming a key link in interrupting pathogen infection. By preventing the formation of filopodia, NF157 effectively blocks the bacterial capture pathway, showing significant intervention effects on some pathogens that lack adhesion devices and flagella and rely on cellular pseudopodia capture for invasion.

This invention belongs to the field of pharmaceutical technology, specifically relating to a novel use of berberine hydrochloride and a pharmaceutical composition. Berberine hydrochloride has the structure shown below, and it significantly inhibits the expression of HMGB3 in triple-negative breast cancer cells, significantly inhibits the proliferation, colony formation, invasion, and migration of triple-negative breast cancer cells, and promotes apoptosis in triple-negative breast cancer cells. Therefore, berberine hydrochloride can be used to prepare drugs for the prevention and / or treatment of triple-negative breast cancer. Furthermore, berberine hydrochloride can enhance the drug sensitivity of cisplatin; the combined use of berberine hydrochloride and cisplatin has a strong synergistic effect in the treatment of triple-negative breast cancer, providing more treatment options for clinical treatment of triple-negative breast cancer.

The application discloses a pteris multifida exosome-like vesicle and application thereof in treatment of prostate cancer and / or bladder cancer. The pteris multifida exosome-like vesicle is prepared by differential ultracentrifugation combined with filter membrane filtration. Further, the pteris multifida exosome-like vesicle is subjected to anti-cancer effect test in multiple prostate cancer and bladder cancer cell lines. The results show that the pteris multifida exosome-like vesicle can effectively inhibit the proliferation, migration and invasion of prostate cancer cells, and block the cell cycle of prostate cancer cells, thereby inhibiting the growth of cancer cells and playing a role in treating prostate cancer. In addition, the pteris multifida exosome-like vesicle can also inhibit the proliferation of bladder cancer cells. It is shown that the pteris multifida exosome-like vesicle can have the treatment effect on prostate cancer and / or bladder cancer, and provides a new strategy and idea for the treatment of prostate cancer and bladder cancer.

The application discloses application of a PI3K-gamma-delta signal path inhibitor in preparation of a product for treating central nervous system leukemia and the product, and solves the technical problem that the prior art lacks a molecular targeting product for acute lymphoblastic leukemia with CNSL which has better effect. The application comprises: application of the PI3K-gamma-delta signal path inhibitor in preparation of a product for treating acute lymphoblastic leukemia with central nervous system leukemia, and application of the PI3K-gamma-delta signal path inhibitor in preparation of a product for inhibiting proliferation, invasion and migration of acute lymphoblastic leukemia with central nervous system leukemia. The application also comprises a product of the PI3K-gamma-delta signal path inhibitor. The application discloses that central nervous system leukemia is closely related to an expression level of MSLN, and the PI3K-gamma-delta signal path inhibitor can inhibit the infiltration degree of acute lymphoblastic leukemia with central nervous system leukemia by reducing the expression level of MSLN, and prolong the survival time.

The invention discloses a preparation method and application of a probiotic-entrapped collagen composite nano-carrier. The preparation method is characterized by comprising the following steps: mixing a fish gelatin protein solution and a polysaccharide solution, adjusting the pH value to be alkaline, heating to carry out glycosylation reaction, and purifying to obtain glycosylated fish gelatin protein. Mixing and emulsifying the glycosylated fish gelatin protein solution serving as a water phase and the oil phase by adopting a reversed-phase emulsification method to form a W / O type emulsion; adding a probiotic bacterial suspension, uniformly stirring, adding a cross-linking agent, carrying out a cross-linking reaction, carrying out solid-liquid separation, washing, and drying, so as to obtain the probiotic-entrapped collagen composite nano-carrier; the preparation method has the advantages that precise and slow-release release in intestinal tracts is realized, and the survival rate and bioavailability of probiotics are remarkably improved.

The application relates to the technical field of biological medicine, and particularly discloses siRNA for targeted inhibition of expression of a circESRP1 gene and application thereof. The application first establishes a human circular RNA hsa_circ_0084927 (circESRP1) as a key target point for treatment of pancreatic cancer, and through rigorous experiments, it is proved that down-regulation of the expression can significantly inhibit proliferation, migration and invasion of pancreatic cancer cells and promote cell apoptosis; based on the specific back-splicing junction (BSJ) structure of the circular RNA, the application designs a specific siRNA molecule crossing the site, realizes efficient (interference efficiency > 85%) and specific gene silencing of the hsa_circ_0084927, and further discloses a new mechanism that the hsa_circ_0084927 promotes immune escape of pancreatic cancer cells by regulating a PD-L1 pathway.

The application relates to the field of medicine, and relates to a beta-carboline chalcone compound as well as a preparation method and application thereof. A series of derivatives are synthesized by taking L-tryptophan methyl ester hydrochloride as raw material, a lead compound with good activity is obtained, a new idea is provided for developing a novel YAP1 inhibitor, and it is proved that the derivatives have a strong inhibitory effect on YAP1.

The utility model relates to the technical field of pest control, in particular to a fly trap with a rainwater-proof structure, which comprises a trapping component, a collecting component is mounted at the bottom of the trapping component, a drainage component is mounted at the bottom of the inner wall of the collecting component, the trapping component comprises a mounting block, a first hanging ring is mounted at the top of the mounting block, and a second hanging ring is mounted at the bottom of the mounting block. A protective cover is welded to the outer wall of the mounting block, a mounting cylinder and a connecting rod are mounted on the inner wall of the protective cover, a connecting rope is mounted at one end of the connecting rod, and a hook is mounted at one end of the connecting rope. According to the improved fly trap, through the collecting cover and the protective cover sprayed with the water-based diamond lotus leaf paint, the effect of shielding rainwater can be achieved, when the rainwater drops on the arc-shaped protective cover sprayed with the water-based diamond lotus leaf paint, the rainwater can slide down along the arc-shaped cover face, and the situation that the rainwater makes contact with components in equipment and erodes the components can be avoided.

The present application relates to the field of molecular biology, and particularly relates to application of a reagent for regulating NDRG2 expression level in preparation of a preparation for treating preeclampsia. 6 A methylation modification level; and reducing the expression level of NDRG2. 6 A methylation modification regulates NDRG2 mRNA stability and further promotes the molecular mechanism of NDRG2 expression. 6 A methylation modification regulates NDRG2 mRNA stability and further promotes the molecular mechanism of NDRG2 expression. Based on this mechanism, by regulating the expression level of NDRG2, the present application effectively inhibits the invasion, migration and proliferation of placental trophoblast cells, promotes the expression of apoptosis and inflammatory factors, which provides a new technical means for targeted treatment of preeclampsia.

This application discloses a laser scanner dust cover, relating to the field of dust covers. It includes a push rod base disposed above a mounting base on the outside of a laser scanner. An electric push rod is provided on one side of the push rod base, and the output end of the electric push rod is connected to the dust cover. A first dustproof cotton is provided on the bottom surface of the dust cover, a second dustproof cotton is provided on the surface of the dust cover, and a third dustproof cotton is provided inside the push rod base. When the laser scanner needs to be used, the electric push rod will activate, causing the dust cover to rise and expose the laser scanner for laser scanning. After use, the electric push rod will activate again, causing the dust cover to descend and cover the laser scanner, providing protection for the scanner head and preventing external wind and sand from damaging it. The first and second dustproof cotton fill the gaps at the connection between the dust cover and the push rod base, and the third dustproof cotton fills the gaps at the connection between the push rod base and the laser scanner, preventing wind and sand from seeping in.

The application discloses poplar phellinus polysaccharide SVP-50-1 and a preparation method and application thereof, and belongs to the field of biological medicines.The poplar phellinus polysaccharide SVP-50-1 is obtained by water extraction and 50% ethanol solution precipitation of poplar phellinus fruiting bodies.The poplar phellinus polysaccharide SVP-50-1 can regulate a tumor metabolic process, inhibit the activity of a tumor cell mitochondrial respiratory chain and an oxidative phosphorylation process, thereby inhibiting the energy source of tumor cells, and achieving an anti-tumor effect.The poplar phellinus polysaccharide SVP-50-1 can play a synergistic effect when combined with radiotherapy and chemotherapy drugs.The combined treatment strategy can significantly improve the curative effect of an existing tumor treatment, while reducing adverse reactions, and provides a new optimized scheme for clinical tumor treatment.

The application provides a bearing with corrosion resistance, relates to the field of mechanical transmission parts, and comprises an outer ring, the inside of the outer ring is provided with a middle ring, the inside of the middle ring is provided with an inner ring, a retainer is arranged between the outer ring and the middle ring, a retainer is also arranged between the middle ring and the inner ring, and the inside of the two retainers is provided with a rolling body. The bearing is formed by the cooperation of the outer ring, the middle ring, the inner ring and the sealing cover, the first sealing cover and the second sealing cover are embedded with the middle ring and the inner ring, a narrow and circuitous sealing channel is formed, the invasion of external moisture, dust and chemical corrosion medium is effectively blocked, the overall corrosion resistance and service life of the bearing in a harsh environment are effectively improved, a dacromet coating is arranged on the exposed surface, physical and electrochemical protection are provided, the bearing can calmly cope with the erosion of high humidity, high salinity and various chemical media, and the corrosion resistance is further improved.

This invention discloses a heparin trisaccharide compound, its preparation method, and its applications, belonging to the field of compound preparation technology. The heparin trisaccharide compound is mainly prepared by the following method: linking a monosaccharide donor and a disaccharide acceptor through glycosylation, then reacting in a methanol / sodium methoxide system or a tetrahydrofuran / sodium hydroxide / water system, followed by sequential O-sulfonation, catalytic hydrogenation, and N-sulfonation post-treatment steps to obtain the heparin trisaccharide compound. This compound has a well-defined structure, a single component, and exhibits anti-angiogenic activity and anti-tumor potential.

This invention discloses an endotracheal intubation assembly for ventilator-associated pneumonia (VAP) protection, comprising an endotracheal cannula and a cannula. The endotracheal cannula includes a cannula body, with first and second air bladders mounted on its lower part, suspended from the ground. The first air bladder is connected to a first inflation port via a first inflation connecting tube, and the second air bladder is connected to a second inflation port via a second inflation connecting tube. A first suction port is located on the cannula body above the first air bladder, and a second suction port is located on the cannula body above the second air bladder. A suction connecting tube fixed to the cannula body has one end connected to a suction port and the other end extending to the locations of the first and second suction ports. A pH sensor is located on the cannula body below the second air bladder. The cannula includes a T-shaped bite pad, consisting of a cylindrical bite pad handle and a sheet-like bite pad head, with a cylindrical isolation membrane extending from the bite pad handle. This invention has advantages such as preventing secretions and pathogens from invading downwards, thus reducing the incidence of VAP.

This invention discloses a ZIF-8 nanomedicine delivery system co-loaded with doxorubicin and siTDRKH, its preparation method, and its applications, belonging to the fields of biomedicine and nanomaterials technology. The nanomedicine delivery system uses the zeolite imidazole ester framework material ZIF-8 as a carrier. Its porous structure encapsulates the chemotherapeutic drug doxorubicin within the framework, and small interfering RNA targeting the TDRKH gene is loaded onto the surface through electrostatic adsorption. This structure exhibits high pH responsiveness: it remains stable under normal physiological conditions (pH 7.4), preventing premature drug leakage; upon entering the slightly acidic environment of a tumor (pH 6.5) or a lysosomal environment (pH 5.0), the ZIF-8 framework immediately disintegrates, achieving targeted and synergistic release of the loaded drug.

The application provides a use of an NR2F2 agonist levomilnacipran in preparation of a drug for treating female renal clear cell carcinoma (ccRCC). In an estrogen environment, the levomilnacipran can significantly promote expression of NR2F2, and significantly inhibit proliferation, invasion and migration of ccRCC cells. The levomilnacipran can be used as a novel therapeutic drug for female ccRCC, and can specifically inhibit progression of the female ccRCC, and has important significance for disease prognosis.

This invention relates to the application of (E)-2-(4-(dimethylamino)styryl)-6-methoxy-3-methylbenzo[d]thiazol-3-onthium in the preparation of anticancer drugs, belonging to the field of pharmaceutical technology. The structure of (E)-2-(4-(dimethylamino)styryl)-6-methoxy-3-methylbenzo[d]thiazol-3-onthium is shown in Formula I below. The (E)-2-(4-(dimethylamino)styryl)-6-methoxy-3-methylbenzo[d]thiazol-3-onthium of this invention can inhibit the growth of gastric cancer cells. By promoting autophagy and mitochondrial fragmentation in gastric cancer cells AGS and HGC-27, it inhibits the proliferation, invasion, and migration of gastric cancer cells and arrests them in the G1 phase. Furthermore, in vivo experiments in mice have confirmed that ZWK-3 can inhibit tumor growth in mice in a significant dose-dependent manner, without affecting mouse body weight or visceral indices. Formula I.

The application belongs to the technical field of medical treatment and specifically relates to a small molecule targeting FOXC1 protein and application thereof. The small molecule targeting FOXC1 protein is N-(4-hydroxy-benzyl)-2-[4-(3-hydroxy-3-phenyl-propenyl)-phenyl]-N-methylacetamide. The small molecule can target FOXC1 protein binding and inhibit migration and invasion of lung cancer cells, and can provide support for preparation of lung cancer metastasis inhibiting drugs and provide a new basis for treatment of lung cancer metastasis.