The invention discloses a dual-component aqueous polyurethane paint with scrawling resistance and pasting resistance, and a preparation method thereof. The dual-component aqueous polyurethane paint with scrawling resistance and pasting resistance is prepared by using aqueous hydroxy-containing polyacrylic resin dispersion and fatty group polyisocyanate as main film forming substances, adding organosilicon resin emulsion, deionized water, antifoaming agent, wetting agent, dispersant, pigment, dulling agent, wax emulsion and thickening agent and carrying out high speed disperse. The paint has characteristics of high crosslinking density, low surface tension, strong permeability resistance, good scratch resistance, substantial scrawling resistance and pasting resistance, excellent performances of low VOC content, high hardness, good weatherability, brushing resistance and chemical resistance, and is suitable for application fields of municipal public facility, building paint and terrace paint coating, etc.