The invention belongs to the field of inorganic antibacterial materials, and discloses a graphene oxide loaded nanometer zinc oxide antibacterial agent, a preparation method and applications thereof. The preparation method comprises the following steps: dissolving a surfactant P123 and graphene oxide in distilled water, adding a NaOH solution, adding solid zinc nitrate, and stirring until the solid is dissolved; carrying out a reaction for 16-18 h at a temperature of 60-85 DEG C under stirring, carrying out centrifugation washing on the obtained reaction solution with distilled water, and collecting precipitate; and dissolving the precipitate in distilled water to obtain a liquid antibacterial agent, or carrying out vacuum drying on the precipitate for 36-48 h at a temperature of 40-75 DEG C to obtain a solid antibacterial agent. According to the present invention, a suitable dispersant is selected to uniformly disperse the nanometer zinc oxide particles on the surface of the graphene oxide, such that the product has good stability; and the graphene oxide loaded nanometer zinc oxide antibacterial agent belongs to a water-soluble inorganic antibacterial agent, such that practical application is convenient, the use process is simple, and characteristics of good stability, good biocompatibility, and good antibacterial property are provided.