59results about How to "Efficient elution" patented technology

A device and method for capture of magnetic beads in a rotary magnetic bead trap is disclosed. The device allows capture, washing, elution and ejection of beads in an automated system. Analyte is eluted in a small volume in a capillary-scale fluid system compatible with LC-MS/MS analysis.

The invention provides an improved method for the purification of nucleic acid molecules, which method comprises generating a cellular lysate containing the nucleic acid; contacting the lysate with an anion exchanger bound to a solid support matrix under conditions such that the anion exchanger binds the nucleic acid; followed by eluting the nucleic acid from the anion exchanger with an aqueous mobile phase comprising an elution solution; and desalting the eluted nucleic acid such that it is suitable for downstream applications. The improvement of the method includes adding in the elution solution a composition such that the pH of the aqueous mobile phase is between about pH 9 and about pH 13, wherein the presence of the composition in the elution solution provides an increase in nucleic acid recovery, as compared with the recovery in the absence of the composition.

A method for cleaning a semiconductor wafer according to the present invention includes the steps of: removing particles on a semiconductor wafer with an alkaline chemical solution to clean the wafer; neutralizing a surface charge of the semiconductor wafer with a weak acid cleaning solution; and removing residual metal impurities on the semiconductor wafer with an acid chemical solution to clean the wafer. The surface of the semiconductor wafer is neutralized and the HPM treatment is then performed with the semiconductor wafer having no charge. As a result, the surface of the semiconductor wafer can be made extremely clean without attaching metal impurities thereto.

Devices for expanded bed chromatography use inlet and outlet ports beneath a mesh that supports sorbent beads in a column. Placement of both ports beneath the mesh provides a horizontal “sweep flow” tangential to the mesh, to suppress the formation of particulate cakes on the lower surface of the mesh when liquids containing high particulate loads (such as cells or cell debris) are being processed. This design can be used with vibrators, hammering devices, intermittent reverse flow, or other means for disrupting the formation of particulate cakes or aggregates. Disrupters can also be used during elution, to accelerate the release of the valuable molecules from the sorbent. Initial tests indicate that these systems can efficiently handle heavily loaded liquids that would rapidly clog other systems previously known in the art.

The invention provides an improved method for the purification of nucleic acid molecules, which method comprises generating a cellular lysate containing the nucleic acid; contacting the lysate with an anion exchanger bound to a solid support matrix under conditions such that the anion exchanger binds the nucleic acid; followed by eluting the nucleic acid from the anion exchanger with an aqueous mobile phase comprising an elution solution; and desalting the eluted nucleic acid such that it is suitable for downstream applications. The improvement of the method includes providing the anion exchanger in a packed column, wherein the column is packed using a salt solution containing an antimicrobial agent. In addition, the salt solution has a salt concentration similar to that of the lysate, such that the column does not need equilibration prior to sample loading.

The invention discloses a method for efficient remediation of mercury-contaminated soil by leaching and a leaching agent. The leaching agent is composed of an oxidizing agent, a leaching solution I and a leaching solution II, wherein the oxidizing agent is a persulfate solution, a solute of the leaching solution I is composed of organic acid, iodized salt and chloride salt, and a solute of the leaching solution II is composed of sodium sulfide and sodium hydroxide. The method for leaching the mercury-contaminated soil by adopting the leaching agent comprises the steps that oxidation treatment is carried out on the mercury-contaminated soil, and then the mercury-contaminated soil is sequentially subjected to leaching by adopting the leaching solution I and the leaching solution II. The method has the advantages that efficient elution of mercury with various valence state in the mercury-contaminated soil can be realized, mercury residues are little, in addition, secondary pollution to the environment can be avoided, and the cost is low.

The present invention provides a strikingly convenient novel purification method of protein, as compared to conventional methods, which affords automation and high throughput, and a material therefor. That is, the present invention provides a magnetic carrier containing a ferromagnetic oxide particle and a carbohydrate layer coating the ferromagnetic oxide particle, a purification method of protein using the carrier, and a reagent kit for purification of protein.

The present invention provides a process for producing an aqueous fluorinated polymer dispersion having a reduced content of a fluorinated emulsifier by using a weakly basic anion-exchange resin to let it adsorb and remove a fluorinated emulsifier with excellent efficiency from an aqueous fluorinated polymer dispersion.

A process for producing an aqueous fluorinated polymer dispersion having a reduced content of a fluorinated emulsifier, which comprises adding an organic carboxylic acid represented by the following formula (1)

Q(CH₂)_m(CH(OH))_nCOOH  (1)

(wherein Q is H, CH₃ or COOH, each of m and n which are independent of each other, is 0 or an integer of from 1 to 4, and 4≧n+m≧1.)

to an aqueous fluorinated polymer dispersion containing a fluorinated emulsifier, followed by contact with a weakly basic anion-exchange resin to adsorb and remove the fluorinated emulsifier.

The invention relates to a case for hydrating a moisture-containing contact lens in a dry condition, a hydrating device, and a hydrating method.

A case (2) comprises a plurality of grooves (8) provided in the inside face (6) of a recess (4). A lens is placed in the case, a proper amount of a hydrating liquid is poured along the inside wall face of the recess (4) in the case in a first step. Then in a second step, a hydrating liquid is poured into the recess from above the case to allow the contact lens (3) to absorb water and swell. Accordingly, a wide extent of a lens front surface can come into contact evenly with the hydrating liquid in the first step, and the entire lens can be hydrated in the second step, whereby an efficient hydration is possible with lens curling and bubble entrapping prevented.

A solid phase extraction (SPE) device having a reservoir with an opening; a well comprising an internally tapered wall, the well having a wider interior diameter at an end closest to the opening than at an exit spout; a first filter within the well; a bed of sorbent particles within the well below the first filter; and a second filter having a diameter smaller than the first filter within the well below the bed of sorbent particles and above the exit spout is provided. A method of performing SPE using the device is also provided.

Disclosed is a process for separating and/or purifying a nucleic acid by elution of the nucleic acid from anion exchange resins under conditions of high salt concentration and the presence in the eluant of an additive comprising guanidine, or a guanidine-like derivative. The process allows high recovery of nucleic acids from anion exchange resins without impairing the nucleic acid stability as compared with conventional ion exchange chromatographic procedures.

The present application relates to a biological reaction device provided with a microfluidic or nano-fluidic structure, characterized in that the biological reaction device comprises a biological sample extraction bin, a reaction bin, and a microfluidic or nano-fluidic structure for transferring a biological sample from the biological sample extraction bin to the reaction bin, and a microfluidic or nano-fluidic structure for transferring the biological sample from the biological sample extraction bin to the reaction bin. Wherein the microfluidic or nano-fluidic structure comprises a biologicalsample channel and a reaction liquid injection channel, wherein the biological sample extraction bin and the reaction bin are fluidly communicated through the biological sample channel, and the reaction liquid is injected into the reaction bin through the reaction liquid injection channel; wherein the microfluidic or nanofluidic structure further comprises a first valve for blocking or opening the biological sample passage and a second valve for blocking or opening the reaction liquid injection passage. The microfluidic or nanofluidic structure further comprises a first valve for blocking oropening the biological sample passage and a second valve for blocking or opening the reaction liquid injection passage. The biological reaction apparatus of the present invention enables accurate quantification of biological samples and sufficient and efficient transfer to the reaction chamber, simplifies the experimental process, improves the experimental efficiency and reduces the likelihood ofcontamination during the transfer of biological samples.

The invention relates to a combined ligand, a combined bionic chromatography medium and a preparation method and application thereof. The combined bionic chromatography medium uses hydrophilic porousmicrospheres as chromatography matrix, is activated by allyl bromide, is brominated and alcoholized by N-bromosuccinimide, and then is coupled with the combined ligand. The sequence of the combined ligand is phenylalanine-tyrosine-glutamic acid-5-aminobenzimidazole. The combined bionic chromatography medium has two functional groups including phenylalanine-tyrosine-glutamic acid tripeptide and aminobenzimidazole, introduces a hydrophobicity charge-inducing ligand while retaining the high selectivity characteristic of a polypeptide ligand to an antibody, makes elution conditions milder, and achieves effective antibody isolation.

The invention discloses an integrated high-efficiency thermal washing-advanced oxidation combined oily sludge treatment system and an oily sludge treatment method, and belongs to the technical field of oily sludge treatment. The method comprises a water washing process and an oxidation process, most crude oil can be efficiently and rapidly eluted in the water washing process, and the degradation difficulty is greatly reduced for subsequent oxidation treatment; in the oxidation process, persulfate is adopted as an oxidizing agent, green and environment-friendly treatment is achieved under the conditions of low temperature and water addition, harm to the environment is small, and an efficient solution is provided for oily sludge remediation.