60results about How to "Efficient elution" patented technology

Methods and devices for a fully automated synthesis of radioactive compounds for imaging, such as by positron emission tomography (PET), in a fast, efficient and compact manner are disclosed. In particular, the various embodiments of the present invention provide an automated, stand-alone, hands-free operation of the entire radiosynthesis cycle on a microfluidic device with unrestricted gas flow through the reactor, starting with target water and yielding purified PET radiotracer within a period of time shorter than conventional chemistry systems. Accordingly, one aspect of the present invention is related to a microfluidic chip for radiosynthesis of a radiolabeled compound, comprising a reaction chamber, one or more flow channels connected to the reaction chamber, one or more vents connected to said reaction chamber, and one or more integrated valves to effect flow control in and out of said reaction chamber.

A device and method for capture of magnetic beads in a rotary magnetic bead trap is disclosed. The device allows capture, washing, elution and ejection of beads in an automated system. Analyte is eluted in a small volume in a capillary-scale fluid system compatible with LC-MS / MS analysis.

The invention provides an improved method for the purification of nucleic acid molecules, which method comprises generating a cellular lysate containing the nucleic acid; contacting the lysate with an anion exchanger bound to a solid support matrix under conditions such that the anion exchanger binds the nucleic acid; followed by eluting the nucleic acid from the anion exchanger with an aqueous mobile phase comprising an elution solution; and desalting the eluted nucleic acid such that it is suitable for downstream applications. The improvement of the method includes adding in the elution solution a composition such that the pH of the aqueous mobile phase is between about pH 9 and about pH 13, wherein the presence of the composition in the elution solution provides an increase in nucleic acid recovery, as compared with the recovery in the absence of the composition.

A simple and efficient method for regenerating a basic anion-exchange resin, whereby at the time of removing a fluorinated emulsifier from a basic anion-exchange resin having the fluorinated emulsifier adsorbed thereon, it is unnecessary to provide a safety device / recovery technique necessary for handling an organic solvent by the use of a combustible organic solvent represented by an alcohol, and a burden imposed by e.g. treatment against COD load, is eliminated.A basic anion-exchange resin having a fluorinated emulsifier adsorbed thereon is contacted with an aqueous alkaline solution having a temperature of from 60 to 105° C. to elute the fluorinated emulsifier thereby to regenerate the basic anion-exchange resin.

A method for cleaning a semiconductor wafer according to the present invention includes the steps of: removing particles on a semiconductor wafer with an alkaline chemical solution to clean the wafer; neutralizing a surface charge of the semiconductor wafer with a weak acid cleaning solution; and removing residual metal impurities on the semiconductor wafer with an acid chemical solution to clean the wafer. The surface of the semiconductor wafer is neutralized and the HPM treatment is then performed with the semiconductor wafer having no charge. As a result, the surface of the semiconductor wafer can be made extremely clean without attaching metal impurities thereto.

Devices for expanded bed chromatography use inlet and outlet ports beneath a mesh that supports sorbent beads in a column. Placement of both ports beneath the mesh provides a horizontal “sweep flow” tangential to the mesh, to suppress the formation of particulate cakes on the lower surface of the mesh when liquids containing high particulate loads (such as cells or cell debris) are being processed. This design can be used with vibrators, hammering devices, intermittent reverse flow, or other means for disrupting the formation of particulate cakes or aggregates. Disrupters can also be used during elution, to accelerate the release of the valuable molecules from the sorbent. Initial tests indicate that these systems can efficiently handle heavily loaded liquids that would rapidly clog other systems previously known in the art.

The invention provides an improved method for the purification of nucleic acid molecules, which method comprises generating a cellular lysate containing the nucleic acid; contacting the lysate with an anion exchanger bound to a solid support matrix under conditions such that the anion exchanger binds the nucleic acid; followed by eluting the nucleic acid from the anion exchanger with an aqueous mobile phase comprising an elution solution; and desalting the eluted nucleic acid such that it is suitable for downstream applications. The improvement of the method includes providing the anion exchanger in a packed column, wherein the column is packed using a salt solution containing an antimicrobial agent. In addition, the salt solution has a salt concentration similar to that of the lysate, such that the column does not need equilibration prior to sample loading.

Disclosed is a process for separating and / or purifying a nucleic acid by elution of the nucleic acid from anion exchange resins under conditions of high salt concentration and the presence in the eluant of an additive comprising guanidine, or a guanidine-like derivative. The process allows high recovery of nucleic acids from anion exchange resins without impairing the nucleic acid stability as compared with conventional ion exchange chromatographic procedures.

The invention discloses a method for efficient remediation of mercury-contaminated soil by leaching and a leaching agent. The leaching agent is composed of an oxidizing agent, a leaching solution I and a leaching solution II, wherein the oxidizing agent is a persulfate solution, a solute of the leaching solution I is composed of organic acid, iodized salt and chloride salt, and a solute of the leaching solution II is composed of sodium sulfide and sodium hydroxide. The method for leaching the mercury-contaminated soil by adopting the leaching agent comprises the steps that oxidation treatment is carried out on the mercury-contaminated soil, and then the mercury-contaminated soil is sequentially subjected to leaching by adopting the leaching solution I and the leaching solution II. The method has the advantages that efficient elution of mercury with various valence state in the mercury-contaminated soil can be realized, mercury residues are little, in addition, secondary pollution to the environment can be avoided, and the cost is low.

The present invention provides a strikingly convenient novel purification method of protein, as compared to conventional methods, which affords automation and high throughput, and a material therefor. That is, the present invention provides a magnetic carrier containing a ferromagnetic oxide particle and a carbohydrate layer coating the ferromagnetic oxide particle, a purification method of protein using the carrier, and a reagent kit for purification of protein.

The present invention relates to a method and means to produce chromatography media having improved pressure-flow properties. More closely, the invention relates to bimodal particle size distribution and the use of layer functionalization as means to change pressure-flow properties of chromatography media. The invention relates to a method for production of chromatography media having improved pressure-flow properties, comprising mixing large beads / particles, comprising an inner core and an outer functionalized shell / lid, with smaller beads / particles, wherein the ratio of the particle size of large and small beads: [D50V for large particles / D50V for small particles]>1.2, and wherein the volume ratio of large and small beads in the column: [Total volume of large beads / Total volume beads] is in the range 0.05-0.9.

The present invention provides a process for producing an aqueous fluorinated polymer dispersion having a reduced content of a fluorinated emulsifier by using a weakly basic anion-exchange resin to let it adsorb and remove a fluorinated emulsifier with excellent efficiency from an aqueous fluorinated polymer dispersion.A process for producing an aqueous fluorinated polymer dispersion having a reduced content of a fluorinated emulsifier, which comprises adding an organic carboxylic acid represented by the following formula (1)Q(CH2)m(CH(OH))nCOOH  (1)(wherein Q is H, CH3 or COOH, each of m and n which are independent of each other, is 0 or an integer of from 1 to 4, and 4≧n+m≧1.)to an aqueous fluorinated polymer dispersion containing a fluorinated emulsifier, followed by contact with a weakly basic anion-exchange resin to adsorb and remove the fluorinated emulsifier.

The invention relates to a case for hydrating a moisture-containing contact lens in a dry condition, a hydrating device, and a hydrating method. A case (2) comprises a plurality of grooves (8) provided in the inside face (6) of a recess (4). A lens is placed in the case, a proper amount of a hydrating liquid is poured along the inside wall face of the recess (4) in the case in a first step. Then in a second step, a hydrating liquid is poured into the recess from above the case to allow the contact lens (3) to absorb water and swell. Accordingly, a wide extent of a lens front surface can come into contact evenly with the hydrating liquid in the first step, and the entire lens can be hydrated in the second step, whereby an efficient hydration is possible with lens curling and bubble entrapping prevented.

The invention relates to a bacterial lysate and a kit for extracting plasmid DNA and a method for extracting the plasmid DNA, and belongs to the technical field of biology. The bacterial lysate for extracting the plasmid DNA comprises lysis buffer, lysozyme and ribonuclease A(RNaseA); wherein100 mL of the lysis buffer comprises 1.5-10 g of sucrose, 0.7-2.2 g of ethylene diamine tetraacetic acid (EDTA), 0.3-0.9 g of tri(hydroxymethyl) amino methane hydrochloride, 2.7-5.4 g of NH4Cl, 0.1-2.0 mL of 5% Triton X-100, 0.2-0.6 g of CaCl2, 0.5-8 g of guanidine hydrochloride, 0.5-3 g of guanidinium isothiocyanate and the balance of water. The kit for extracting the plasmid DNA comprises the bacterial lysate, rinsing solution and eluant and is low in cost, high in purity of extracted plasmid and high in applicability.

A solid phase extraction (SPE) device having a reservoir with an opening; a well comprising an internally tapered wall, the well having a wider interior diameter at an end closest to the opening than at an exit spout; a first filter within the well; a bed of sorbent particles within the well below the first filter; and a second filter having a diameter smaller than the first filter within the well below the bed of sorbent particles and above the exit spout is provided. A method of performing SPE using the device is also provided.

Disclosed is a process for separating and / or purifying a nucleic acid by elution of the nucleic acid from anion exchange resins under conditions of high salt concentration and the presence in the eluant of an additive comprising guanidine, or a guanidine-like derivative. The process allows high recovery of nucleic acids from anion exchange resins without impairing the nucleic acid stability as compared with conventional ion exchange chromatographic procedures.

The present application relates to a biological reaction device provided with a microfluidic or nano-fluidic structure, characterized in that the biological reaction device comprises a biological sample extraction bin, a reaction bin, and a microfluidic or nano-fluidic structure for transferring a biological sample from the biological sample extraction bin to the reaction bin, and a microfluidic or nano-fluidic structure for transferring the biological sample from the biological sample extraction bin to the reaction bin. Wherein the microfluidic or nano-fluidic structure comprises a biologicalsample channel and a reaction liquid injection channel, wherein the biological sample extraction bin and the reaction bin are fluidly communicated through the biological sample channel, and the reaction liquid is injected into the reaction bin through the reaction liquid injection channel; wherein the microfluidic or nanofluidic structure further comprises a first valve for blocking or opening the biological sample passage and a second valve for blocking or opening the reaction liquid injection passage. The microfluidic or nanofluidic structure further comprises a first valve for blocking oropening the biological sample passage and a second valve for blocking or opening the reaction liquid injection passage. The biological reaction apparatus of the present invention enables accurate quantification of biological samples and sufficient and efficient transfer to the reaction chamber, simplifies the experimental process, improves the experimental efficiency and reduces the likelihood ofcontamination during the transfer of biological samples.

The invention provides a method for treating wastewater with a conductive filter material and achieving regeneration. The conductive filter material is made into a pipe shape suitable for pressure filtering, namely, a filter pipe, and the filter pipe is connected with the negative electrode of an electrolytic power supply; an inert electrode is arranged in the center of the filter pipe and connected with the positive electrode of the electrolytic power supply; one end of the filter pipe is provided with a water inlet pipe for pumping in wastewater to be treated, and the other end of the filter pipe is provided with a water outlet pipe. In the filtering process, the electrolytic power supply carries out power supply and electrolysis at the same time, or when the water outlet amount is obviously decreased after filtering is carried out for a period of time, electrolysis is started to recover the filtering performance of the filter pipe. In the electrolysis process, hydrogen bubbles are constantly generated in pore channels of the filter material serving as a negative electrode and generate huge bubble pressure in the growing process, so that particles filling the pore channels of the filter material are rapidly separated from a biological membrane, and the filtering performance of the filter material is rapidly recovered. The method solves the problems that a conventional filter material is difficult to regenerate, short in service life and the like.

The present invention provides a process for preparing a polycrystalline silicon having the surface layer in which the areas having a short carrier lifetime due to Fe has been substantially eliminated. A preparation method of polycrystalline silicon comprising preparing a mold evenly applied with a mold release agent produced by mixing a binder and a solvent with a silicon nitride powder and then solidifying a molten silicon in said mold, wherein x≦5.0, 20≦y≦100 and x×y≦100 are satisfied given that x represents a concentration of Fe (atomic ppm) contained as impurity in the silicon nitride powder and y represents a thickness of the mold release agent (μm) applied to the mold.

The invention discloses a pretreatment method for the ultrasonic extraction of multiple antibiotics in a sediment. The pretreatment method comprises the following steps: 1, freeze-drying the sedimentor sandy soil, pulverizing, and screening by a 200-mesh sieve to obtain a sample; 2, putting the sample obtained in the step 1 and an internal standard liquid in a centrifuge tube; 3, adding an extraction agent into the centrifuge tube, conducting ultrasonic treatment for 15-30 minutes, then centrifuging, obtaining a liquid supernatant, and repeating for at least twice; 4, evaporating the liquid supernatant obtained in the step 3 until the volume no longer changes, then redissolving with ultrapure water to 300 mL, and regulating the pH to 2-9 to obtain a water sample; and 5, extracting the sample. The pretreatment method is relatively low in cost, and overcomes the defects of loss of the antibiotics caused by relatively poor stability of the antibiotics and relatively high temperature in the accelerated solvent extraction process.

The invention relates to a combined ligand, a combined bionic chromatography medium and a preparation method and application thereof. The combined bionic chromatography medium uses hydrophilic porousmicrospheres as chromatography matrix, is activated by allyl bromide, is brominated and alcoholized by N-bromosuccinimide, and then is coupled with the combined ligand. The sequence of the combined ligand is phenylalanine-tyrosine-glutamic acid-5-aminobenzimidazole. The combined bionic chromatography medium has two functional groups including phenylalanine-tyrosine-glutamic acid tripeptide and aminobenzimidazole, introduces a hydrophobicity charge-inducing ligand while retaining the high selectivity characteristic of a polypeptide ligand to an antibody, makes elution conditions milder, and achieves effective antibody isolation.

The invention discloses an integrated high-efficiency thermal washing-advanced oxidation combined oily sludge treatment system and an oily sludge treatment method, and belongs to the technical field of oily sludge treatment. The method comprises a water washing process and an oxidation process, most crude oil can be efficiently and rapidly eluted in the water washing process, and the degradation difficulty is greatly reduced for subsequent oxidation treatment; in the oxidation process, persulfate is adopted as an oxidizing agent, green and environment-friendly treatment is achieved under the conditions of low temperature and water addition, harm to the environment is small, and an efficient solution is provided for oily sludge remediation.

The invention discloses a water body phosphate in-situ rapid enrichment device which comprises a chromatographic column, the chromatographic column is fixed on a chromatographic column frame, the inner bottom of the chromatographic column is filled with zirconium-loaded zeolite, rubber tubes are connected to the top and the bottom of the chromatographic column, and a water outlet is formed in thefront end of the rubber tube at the bottom of the chromatographic column. The front end of the rubber tube at the top of the chromatographic column is connected with the output end of a small rechargeable water pump, the input end of the small rechargeable water pump is also connected with a rubber tube, the front end of the rubber tube at the input end of the small rechargeable water pump is a water inlet, and the water inlet is wrapped with non-woven filter cloth; the device disclosed by the invention can realize in-situ rapid enrichment and laboratory efficient elution of phosphate in rivers or lakes, and finally a high-purity and high-concentration phosphate solution is obtained, so that subsequent generation of high-purity Ag3PO4 is facilitated; compared with a traditional phosphate oxygen isotope pretreatment method, collection and transportation of a large number of overlying water samples can be avoided.