The application discloses a method for extracting soybean saponins and soybean oligosaccharides, and belongs to the technical field of
natural product extraction. The method comprises the following steps: puffing treatment of
soybean meal, wall breaking treatment in a high-
voltage electric field; acid
precipitation, collection of supernatant; after
ultrasonic oscillation of the supernatant, a composite
enzyme is added for enzymolysis, and a material liquid after enzymolysis is obtained; after
filtration of the material liquid, the material liquid is loaded onto a
macroporous resin column for adsorption, and after completion of the adsorption,
effluent is collected; the
effluent is purified and concentrated to obtain soybean oligosaccharides; an
ethanol solution is used to elute the resin column, and eluate is collected; the eluate is recovered by
ethanol and dried to obtain soybean saponins; the composite
enzyme comprises
amylase and beta-
glucosidase. The method can significantly improve the extraction rate and purity of soybean saponins and soybean oligosaccharides, the whole process uses water as the main
solvent, the consumption of organic solvents and the
pollution to the environment are reduced, and the
unit process water consumption, unit
energy consumption and
unit cost are reduced.