Embodiments relate to systems and methods for interrogating one or more diagnostic targets using a remotely loaded image. A set of managed machines, such as personal computers or servers, can be managed by a network management engine communicating with the machines via a secure channel. The network management engine can access a selected diagnostic target to temporarily deactivate the installed native operating system on that target, insert a substitute operating environment, and remotely interrogate the target to perform hardware, software, security, or other diagnostics or installs. The network management engine can connect to the diagnostic target, extract security keys from the native operating environment, remotely load the substitute environment, and then perform diagnostic or provisioning activities while the native environment is deactivate and the diagnostic target is effectively off-network. Installations, diagnostics, or other activities that require the diagnostic target to be isolated from other machines can therefore be safely conducted.