This invention belongs to the field of bio-
fermentation and skincare technology, specifically relating to a coffee
fermentation product, its preparation method, and its application. The preparation method is as follows:
green coffee beans are ground into
powder, sieved, mixed with water, and then treated with a compound
enzyme for
enzymatic hydrolysis. Then, fructooligosaccharides and vitamins are added, mixed thoroughly, and sterilized to obtain culture medium 1.
Coffee grounds are homogenized with
green tea powder,
sucrose, and water, and sterilized to obtain culture medium 2. A compound of *
Bifidobacterium breve* and *
Saccharomyces cerevisiae* is inoculated into culture medium 1 for
fermentation. Then, culture medium 2 is added, and *
Lactobacillus plantarum* is inoculated for further fermentation. After fermentation, the residue
powder is removed by
filtration to obtain a fermentation filtrate. Acidic
protease is added to the obtained fermentation filtrate for
enzymatic hydrolysis, followed by
centrifugation, sterilization, and
filtration to obtain the coffee fermentation product. This invention, by using
green coffee beans and
coffee grounds for synergistic fermentation and using
green tea powder as an auxiliary fermentation component, can synergistically enhance the soothing and
antioxidant effects of the coffee fermentation product.