The invention discloses a novel pesticide, and a preparation method and an application thereof, and belongs to the technical field of preparation of pesticides. The novel pesticide comprises, by weight, leaves of Salix babylonica.L, Piper methysticum Forst, Camellia Sinensis leaf, Anabasis aphylla L., paulownia leaf, Derris fordii Oliv., garlic, neem, horseradish, Pterocarya stenoptera leaves, Cortex Fraxini, Sophora flavescens, Euphorbia soongarica, pyrethrum, onion, patrinia herb, purslane, Radix Scutellariae, aloe, Cortex Magnoliae Officinalis and water, and the novel pesticide is prepared through the steps of medicine solution extraction, concentration, drying, crushing, sieving and disinfection. The novel pesticide has the advantages of nontoxic component, no residue, no nuisance, simple production method, low cost and good application effect, can effectively prevent and treat yellow spiders, Parlatoria pergandii Comstock, papilios, leaf miner and other plant pests, has a primary killing rate reaching 96.06% or above, has no medicine resistance, and can be used for 4-6 years.