The invention belongs to the technical field of
molecular biology, and particularly relates to a
molecular marker primer group for identifying huperzia longibrachiata and huperzia rugulosa and application of the
molecular marker primer group. The invention provides a cpSSR primer group for identifying huperzia longibrachiata and huperzia rugulosa, the cpSSR marker primer group comprises one or more of Hup3, Hup15, Hup22 or Hup26, and the huperzia longibrachiata and huperzia rugulosa can be accurately identified by adopting
chloroplast SSR marker primers. The method has the advantages of simplicity and convenience in operation, low cost, high
repeatability and the like, and can be widely applied to true and false identification of traditional Chinese medicinal materials, species resource investigation and
molecular breeding research.