7 results about "Brucea" patented technology

The application belongs to the technical field of molecular biology, and particularly relates to a molecular marker primer group for identifying Huperzia serrata and H. phlegmaria and application thereof. The application provides a cpSSR primer group for identifying Huperzia serrata and H. phlegmaria, wherein the cpSSR marker primer group comprises one or more of Hup3, Hup15, Hup22 or Hup26. The chloroplast SSR marker primer can be used to accurately identify Huperzia serrata and H. phlegmaria. The method has the advantages of simple operation, low cost, high repeatability and the like, and can be widely applied to identification of true and false traditional Chinese medicinal materials, investigation of species resources and molecular breeding research.

The invention relates to the technical field of traditional Chinese medicine preparation, in particular to a traditional Chinese medicine composition for inhibiting HPV infection and a preparation method and application thereof. The traditional Chinese medicine composition is prepared from the following raw materials: rhizoma smilacis glabrae, radix sophorae flavescentis, herba houttuyniae, honeycomb, dysosma versipellis, fructus cnidii and brucea javanica. The preparation method comprises the following steps: (1) crushing brucea javanica, and preparing brucea javanica oil through supercritical extraction; (2) mixing fructus cnidii and dysosma versipellis to obtain a mixture A, adding ethanol for extraction, and performing solid-liquid separation to obtain an alcohol extract; (3) mixing radix sophorae flavescentis, herba houttuyniae, rhizoma smilacis glabrae and nidus vespae to obtain a mixture B, adding water for extraction, and performing solid-liquid separation to obtain a water extract; and (4) uniformly mixing the brucea javanica oil, the alcohol extract and the water extract. Liquid carbon dioxide and a deep-eutectic solvent are adopted in the supercritical extraction, and the deep-eutectic solvent is composed of quaternary ammonium salt and organic acid. The traditional Chinese medicine composition prepared by the invention is better in HPV infection inhibition effect and lower in side effect.

This invention discloses alkaloid compounds from Brucea javanica, their preparation methods, and applications, belonging to the field of pharmaceutical technology. Specifically, it relates to two alkaloid compounds extracted and isolated from the fruit of Brucea javanica, a plant of the genus Brucea in the family Simaroubaceae. These compounds have good acetylcholinesterase inhibitory activity and can be used to prepare acetylcholinesterase inhibitors, showing good development prospects and application potential.

The invention belongs to the technical field of molecular biology, and particularly relates to a molecular marker primer group for identifying huperzia longibrachiata and huperzia rugulosa and application of the molecular marker primer group. The invention provides a cpSSR primer group for identifying huperzia longibrachiata and huperzia rugulosa, the cpSSR marker primer group comprises one or more of Hup3, Hup15, Hup22 or Hup26, and the huperzia longibrachiata and huperzia rugulosa can be accurately identified by adopting chloroplast SSR marker primers. The method has the advantages of simplicity and convenience in operation, low cost, high repeatability and the like, and can be widely applied to true and false identification of traditional Chinese medicinal materials, species resource investigation and molecular breeding research.

The invention relates to triterpenoids in brucea javanica branches and leaves as well as a preparation method and application of the triterpenoids, belongs to the technical field of medicines, and particularly relates to nine triterpenoids extracted and separated from brucea javanica branches and leaves (Brucea javanica) of a picraliaceae brucea plant. A biological activity experiment result shows that the compound 4 has good anti-inflammatory activity. In addition, the preparation method disclosed by the invention is simple and easy to implement, relatively good in reproducibility and relatively high in product purity.

The present application belongs to the field of agricultural fungicides, and particularly relates to a fungicidal composition containing nano-zinc oxide and brucea extract and application thereof, wherein the mass ratio of the nano-zinc oxide and the brucea extract in the active ingredient of the fungicidal composition is 40:1-1:40; the fungicidal composition has excellent fungicidal effect, and shows excellent prevention and control effect on watermelon fusarium wilt, wheat foot rot, tobacco bacterial wilt, cotton damping-off, cotton sudden collapse, peanut root rot, peanut stem base rot, and damping-off, fusarium wilt, sudden collapse, root rot and the like of fruits, vegetables and legumes, and is environment-friendly, safe to crops, and has good application prospect while enhancing the broad spectrum and efficiency of fungicides.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a compound traditional Chinese medicine composition for treating skin warts and condyloma and application thereof. The compound traditional Chinese medicine composition is prepared from the following raw material components: astilbin, sophocarpidine, herba houttuyniae, honeycomb, podophyllotoxin, fructus cnidii and brucea javanica. The preparation method comprises the following steps: firstly, squeezing the brucea javanica to extract brucea javanica oil to obtain decoction dregs; the preparation method comprises the following steps: adding water into herba houttuyniae, nidus vespae, fructus cnidii and medicine residues, decocting to obtain decoction, adding tannase into the decoction for enzymolysis to obtain enzymatic hydrolysate, inactivating, and finally mixing and dispersing the enzymatic hydrolysate, brucea javanica oil, astilbin, sophocarpidine and podophyllotoxin uniformly to obtain the medicine. All the components are compatible, and the synergistic effect is remarkably superior to that of an original traditional Chinese medicine prescription; the traditional Chinese medicine composition has a good application market prospect for treating skin warts and condyloma.