The present application belongs to the technical field of
aquaculture, and discloses a compound synergist of
neomycin sulfate as well as a preparation method and application thereof. The present application discloses, for the first time, the application of
Wedelia prostrata extract,
Gastrodia extract and Picrasma extract in inhibiting the growth of
Vibrio in vitro and in preventing and treating the glass fry
disease of
South American white
shrimp caused by
Vibrio infection. Experimental
verification shows that the
Wedelia prostrata extract,
Gastrodia extract and Picrasma extract have good effects in inhibiting the growth of
Vibrio in vitro when used alone, in combination with each other or simultaneously. Meanwhile, in view of the treatment of Vibrio infection causing the glass fry
disease, the above extracts are used in combination with
neomycin sulfate, so as to overcome the objective problem of low or weak
efficacy of
neomycin sulfate at a recommended
dose, and to simultaneously achieve the enhancement of the
efficacy of
neomycin sulfate after being used in combination with
neomycin sulfate, and the reduction of the usage amount of
neomycin sulfate.