36 results about "Brucea javanica" patented technology

The invention belongs to the technical field of medicines, and particularly relates to a nitroimidazole brucea javanica bitter alcohol derivative with a low-oxygen activation prodrug property in dry fruits of a medicinal plant brucea javanica, and a preparation method and an application of the nitroimidazole brucea javanica bitter alcohol derivative. According to the invention, a series of novel brucea javanica bitter alcohol derivatives or pharmaceutically acceptable salts thereof are designed and synthesized by carrying out necessary structural modification on brucea javanica bitter alcohol. Anti-tumor drug screening on digestive system cancer cell proliferation through pharmacological experiments shows that the brucea javanica bitter alcohol derivative or the pharmaceutically acceptable salt thereof has good colon cancer cell inhibition activity, so that the brucea javanica bitter alcohol derivative or the pharmaceutically acceptable salt thereof can be developed into a new anti-colon cancer drug; and the method has very high potential research value and clinical application prospect.

The invention belongs to the field of agricultural bactericides, and particularly relates to a bactericidal composition containing nano-zinc oxide and brucea javanica extract and application of the bactericidal composition, and the mass ratio of the nano-zinc oxide to the brucea javanica extract in active ingredients of the bactericidal composition is 40: 1-1: 40; the bactericidal composition disclosed by the invention has an excellent bactericidal effect, and shows excellent control effects on watermelon fusarium wilt, wheat stem rot, tobacco bacterial wilt, cotton damping-off, cotton damping-off, peanut root rot, peanut stem rot, damping-off, fusarium wilt, damping-off, root rot and the like of fruits, vegetables and beans; the bactericidal composition enhances the bactericidal broad-spectrum property and efficiency, is environment-friendly, does not generate phytotoxicity, is safe to crops, and has a good application prospect.

The invention discloses a traditional Chinese medicine composition for treating pneumonia and a preparation method thereof, and relates to the technical field of traditional Chinese medicines. According to the traditional Chinese medicine composition for treating pneumonia and the preparation method thereof, the traditional Chinese medicine composition for treating pneumonia comprises the following raw materials: honeysuckle, cordyceps militaris, cornu gorais, bear gall powder, fructus forsythiae, astragalus membranaceus, brucea javanica, fructus psoraleae, ginseng, cinnamon, ginger and honey-fried licorice root. Based on traditional Chinese medicine theories and pneumonia pathogenesis characteristics, and on the basis of common honeysuckle, fructus forsythiae and astragalus membranaceus for treating pneumonia, cordyceps militaris, brucea javanica, ginseng, cornu gorais and other traditional Chinese medicine components are added, so that combined use of multiple traditional Chinese medicines is realized; the traditional Chinese medicine composition can improve the treatment effect, clear and moisten lung dryness, improve fever, cough, chest distress, breath promotion and other symptoms of pneumonia patients, is good in safety, has multiple taking forms, and is suitable for most patients to use.

The invention relates to the technical field of skin care, and discloses pure plant compound liquid for removing warts and repairing a skin cuticle barrier and a preparation method of the pure plant compound liquid. The compound liquid is prepared by compounding a purslane extract, a brucea javanica extract, a honeysuckle extract, a licorice extract, an aloe extract, hyaluronic acid, glycerin, menthol and deionized water according to specific parts by weight. The preparation method comprises the steps of raw material pretreatment, basic liquid preparation, active ingredient addition, seasoning and homogenization, filtration and sterilization and the like. Whole plant extracts are adopted as core active ingredients, the synergistic effect of resisting viruses, removing warts, resisting inflammation and repairing skin barriers is achieved through scientific compounding, the activity of human papilloma viruses can be efficiently inhibited, wart shedding is promoted, damaged cuticles can be repaired, and skin resistance can be enhanced; the product is free of chemical preservatives and corrosive components, high in safety, small in irritation, suitable for various skin types, simple in preparation process and suitable for industrial large-scale production.

The application belongs to the technical field of molecular biology, and particularly relates to a molecular marker primer group for identifying Huperzia serrata and H. phlegmaria and application thereof. The application provides a cpSSR primer group for identifying Huperzia serrata and H. phlegmaria, wherein the cpSSR marker primer group comprises one or more of Hup3, Hup15, Hup22 or Hup26. The chloroplast SSR marker primer can be used to accurately identify Huperzia serrata and H. phlegmaria. The method has the advantages of simple operation, low cost, high repeatability and the like, and can be widely applied to identification of true and false traditional Chinese medicinal materials, investigation of species resources and molecular breeding research.

The invention relates to the technical field of traditional Chinese medicine preparation, in particular to a traditional Chinese medicine composition for inhibiting HPV infection and a preparation method and application thereof. The traditional Chinese medicine composition is prepared from the following raw materials: rhizoma smilacis glabrae, radix sophorae flavescentis, herba houttuyniae, honeycomb, dysosma versipellis, fructus cnidii and brucea javanica. The preparation method comprises the following steps: (1) crushing brucea javanica, and preparing brucea javanica oil through supercritical extraction; (2) mixing fructus cnidii and dysosma versipellis to obtain a mixture A, adding ethanol for extraction, and performing solid-liquid separation to obtain an alcohol extract; (3) mixing radix sophorae flavescentis, herba houttuyniae, rhizoma smilacis glabrae and nidus vespae to obtain a mixture B, adding water for extraction, and performing solid-liquid separation to obtain a water extract; and (4) uniformly mixing the brucea javanica oil, the alcohol extract and the water extract. Liquid carbon dioxide and a deep-eutectic solvent are adopted in the supercritical extraction, and the deep-eutectic solvent is composed of quaternary ammonium salt and organic acid. The traditional Chinese medicine composition prepared by the invention is better in HPV infection inhibition effect and lower in side effect.

The invention relates to the technical field of marine antifouling, in particular to a brusatol derivative and application of the brusatol derivative in marine biofouling resistance, and structurally modifies brusatol, which is one of main compounds in plant brucea javanica with wide sources, by utilizing a directional multi-target ligand strategy to obtain the brusatol derivative. Strong anti-pollution active groups (halogen, cyano groups, benzene rings, heterocyclic rings and the like) derived from the ocean are hybridized on a brusatol mother nucleus molecule, and a series of novel brusatol derivatives hybridized with the strongly anti-pollution active groups derived from the ocean are successfully synthesized. The compounds show good anti-barnacle adhesion activity in anti-adhesion experiments of barnacles, and have low toxicity to target organisms. According to the method, the variety of candidate compounds of the anti-fouling agent is enriched, a new way is provided for developing novel and efficient anti-fouling coatings, and the method has good application prospects and industrial value.

The invention discloses a brucea javanica bitter alcohol quinoline derivative as well as a preparation method and application thereof, and relates to the technical field of medicinal chemistry and tumor treatment. The structural formula of the brucea javanica bitter alcohol quinoline derivative is as shown in a formula (I): # imgabs0 #; r in the formula (I) is a substituted quinoline acyl group, the brucea javanica quinoline derivative prepared by introducing a quinoline group into a brucea javanica mother nucleus has high anti-tumor activity, strong target spot selectivity and excellent chemical stability and metabolic stability, provides a novel candidate drug for tumor treatment, and has broad application prospects. The application potential in tumor treatment is obviously improved; the brucea javanica bitter alcohol quinoline derivative is prepared by one step through esterification reaction, and the preparation method is simple and easy to operate.

The invention belongs to the technical field of medicines, and particularly relates to a triphenylphosphine brucea javanica bitter alcohol derivative with anti-tumor activity in dry fruits of a medicinal plant brucea javanica, and a preparation method and application of the triphenylphosphine brucea javanica bitter alcohol derivative. According to the invention, a series of brucea javanica triphenylphosphine derivatives or pharmaceutically acceptable salts thereof are designed and synthesized by carrying out necessary structural modification on brucea javanica. Anti-tumor drug screening on digestive system cancer cell proliferation through pharmacological experiments shows that the brucea javanica bitter alcohol derivative or the pharmaceutically acceptable salt thereof has good gastric cancer cell inhibition activity, so that the brucea javanica bitter alcohol derivative or the pharmaceutically acceptable salt thereof can be developed into new anti-gastric cancer drugs and has very high potential research value and clinical application prospect.

This invention discloses alkaloid compounds from Brucea javanica, their preparation methods, and applications, belonging to the field of pharmaceutical technology. Specifically, it relates to two alkaloid compounds extracted and isolated from the fruit of Brucea javanica, a plant of the genus Brucea in the family Simaroubaceae. These compounds have good acetylcholinesterase inhibitory activity and can be used to prepare acetylcholinesterase inhibitors, showing good development prospects and application potential.

The invention discloses a plant composite antibacterial liquid composition aiming at HPV (human papillomavirus) infection and a preparation method thereof, and belongs to the technical field of biological medicines. The composition is prepared from the following components in parts by weight: 5 to 20 parts of brucea javanica extract, 3 to 15 parts of senna extract, 2 to 10 parts of anhydridized beta-lactoglobulin, 1 to 8 parts of dandelion extract, 1 to 5 parts of penetration enhancer, 0.1 to 1 part of pH (Potential of Hydrogen) regulator, 0.1 to 0.5 part of preservative and the balance of phosphate buffer solution. According to the invention, anhydridized beta-lactoglobulin is innovatively taken as a carrier, so that targeted delivery of active ingredients is realized; the anti-HPV effect is enhanced through the synergistic effect of the brucea javanica and the kalii extract; the anti-inflammatory repair function of the dandelion extract is combined, and a compound penetration enhancer and a specific preparation process are matched, so that the product has the characteristics of efficient HPV resistance, anti-inflammatory repair, mildness, no irritation and good stability.

The invention belongs to the technical field of molecular biology, and particularly relates to a molecular marker primer group for identifying huperzia longibrachiata and huperzia rugulosa and application of the molecular marker primer group. The invention provides a cpSSR primer group for identifying huperzia longibrachiata and huperzia rugulosa, the cpSSR marker primer group comprises one or more of Hup3, Hup15, Hup22 or Hup26, and the huperzia longibrachiata and huperzia rugulosa can be accurately identified by adopting chloroplast SSR marker primers. The method has the advantages of simplicity and convenience in operation, low cost, high repeatability and the like, and can be widely applied to true and false identification of traditional Chinese medicinal materials, species resource investigation and molecular breeding research.

The present invention provides a method for preparing Brucea javanica oil composition and its application. The Brucea javanica oil composition includes refined soybean phospholipid and Brucea javanica oil. Among them, the iodine value of the refined soybean phospholipid is not less than 90 and not more than 100. When the Brucea javanica oil composition prepared by the method is clinically applied within 18 months, the risk of hemolysis is significantly reduced, and the quality stability and product safety are significantly improved.

The invention provides a traditional Chinese medicine composition for treating viral warts as well as a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition is prepared from the following components in parts by mass: 20 to 30 parts of herba portulacae, 15 to 25 parts of radix isatidis, 25 to 35 parts of herba equiseti hiemalis, 25 to 35 parts of raw rhizoma cyperi, 5 to 15 parts of radix arnebiae seu lithospermi, 5 to 15 parts of herba patriniae, 1 to 5 parts of flos carthami, 15 to 25 parts of mirabilite and 5 to 15 parts of fructus bruceae. The traditional Chinese medicine composition provided by the invention has a good curative effect on viral warts, especially on palmoplantar warts and perineal warts, has the advantages of small side effects and difficulty in relapse, and is an ideal medicine for treating the palmoplantar warts and the perineal warts.

The invention relates to triterpenoids in brucea javanica branches and leaves as well as a preparation method and application of the triterpenoids, belongs to the technical field of medicines, and particularly relates to nine triterpenoids extracted and separated from brucea javanica branches and leaves (Brucea javanica) of a picraliaceae brucea plant. A biological activity experiment result shows that the compound 4 has good anti-inflammatory activity. In addition, the preparation method disclosed by the invention is simple and easy to implement, relatively good in reproducibility and relatively high in product purity.

The invention relates to the technical field of biological medicines, and particularly discloses application of brucea javanica oil in preparation of a medicine for inhibiting radiation pneumonitis or idiopathic pulmonary fibrosis. When the brucea javanica oil is used for preparing the medicine for inhibiting radiation pneumonitis, the symptom of radiation pneumonitis can be remarkably relieved, the curative effect of the medicine is equivalent to that of dexamethasone, release of proinflammatory factors can be effectively inhibited, and inflammatory cascade reaction is blocked and lung tissue injury is relieved by down-regulating mRNA and protein expression of an HMGB1 / TLR4 / NF-kappa BP65 axis. The brucea javanica oil is applied to preparation of the medicine for treating idiopathic pulmonary fibrosis, lung tissue inflammatory cell infiltration can be effectively inhibited, abnormal collagen deposition can be reduced, expression of fibrosis promoting proteins COL1A1, VIM and ACTA2 can be down-regulated, and the pulmonary fibrosis process can be relieved by inhibiting fibroblast activation and extracellular matrix excessive deposition.

The present application belongs to the field of agricultural fungicides, and particularly relates to a fungicidal composition containing nano-zinc oxide and brucea extract and application thereof, wherein the mass ratio of the nano-zinc oxide and the brucea extract in the active ingredient of the fungicidal composition is 40:1-1:40; the fungicidal composition has excellent fungicidal effect, and shows excellent prevention and control effect on watermelon fusarium wilt, wheat foot rot, tobacco bacterial wilt, cotton damping-off, cotton sudden collapse, peanut root rot, peanut stem base rot, and damping-off, fusarium wilt, sudden collapse, root rot and the like of fruits, vegetables and legumes, and is environment-friendly, safe to crops, and has good application prospect while enhancing the broad spectrum and efficiency of fungicides.

The present invention relates to indole alkaloids from Brucea javanica, their preparation methods, and applications, belonging to the field of pharmaceutical technology. Specifically, the invention relates to two indole alkaloid derivatives extracted and isolated from Brucea javanica, a plant of the genus Simarthaceae. These derivatives exhibit excellent anti-tumor activity. The preparation method of the present invention is simple and easy to implement, has good reproducibility, and is of high purity. #imgabs0#

The application belongs to the technical field of traditional Chinese medicine, and particularly relates to a compound traditional Chinese medicine composition for treating skin warts and condyloma and application thereof. Raw materials of the compound traditional Chinese medicine composition comprise the following components: astilbin, sophoramine, houttuynia cordata, honeycomb, podophyllotoxin, cumin and brucea javanica. A preparation method comprises the following steps: first, extracting brucea javanica oil by pressing brucea javanica to obtain residues; then, adding houttuynia cordata, honeycomb, cumin and the residues into water to obtain decoction by boiling, adding tannase into the decoction to obtain enzyme hydrolysate by enzymolysis, inactivating, and finally, uniformly mixing and dispersing the enzyme hydrolysate, brucea javanica oil, astilbin, sophoramine and podophyllotoxin to obtain the compound traditional Chinese medicine composition. The application has the synergistic effect which is significantly better than that of the original traditional Chinese medicine prescription, and has a good application market prospect for treating skin warts and condyloma.

The present application relates to the technical field of traditional Chinese medicine preparation, in particular to a traditional Chinese medicine composition for inhibiting HPV infection, a preparation method and application thereof. The traditional Chinese medicine composition is prepared from the following raw materials: smilax glabra, sophora flavescens, houttuynia cordata, honeycomb, dysphania, cnidium and brucea javanica; the preparation method comprises the following steps: (1) taking brucea javanica, crushing it and then preparing brucea javanica oil through supercritical extraction; (2) mixing cnidium and dysphania to obtain mixture A, adding ethanol for extraction, and then performing solid-liquid separation to obtain an alcohol extract; (3) mixing sophora flavescens, houttuynia cordata, smilax glabra and honeycomb to obtain mixture B, adding water for extraction, and then performing solid-liquid separation to obtain a water extract; (4) uniformly mixing the brucea javanica oil, the alcohol extract and the water extract to obtain the traditional Chinese medicine composition. The supercritical extraction adopts liquid carbon dioxide and a eutectic solvent, and the eutectic solvent is composed of a quaternary ammonium salt and an organic acid. The traditional Chinese medicine composition prepared by the present application has better HPV infection inhibition effect and lower side effects.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a compound traditional Chinese medicine composition for treating skin warts and condyloma and application thereof. The compound traditional Chinese medicine composition is prepared from the following raw material components: astilbin, sophocarpidine, herba houttuyniae, honeycomb, podophyllotoxin, fructus cnidii and brucea javanica. The preparation method comprises the following steps: firstly, squeezing the brucea javanica to extract brucea javanica oil to obtain decoction dregs; the preparation method comprises the following steps: adding water into herba houttuyniae, nidus vespae, fructus cnidii and medicine residues, decocting to obtain decoction, adding tannase into the decoction for enzymolysis to obtain enzymatic hydrolysate, inactivating, and finally mixing and dispersing the enzymatic hydrolysate, brucea javanica oil, astilbin, sophocarpidine and podophyllotoxin uniformly to obtain the medicine. All the components are compatible, and the synergistic effect is remarkably superior to that of an original traditional Chinese medicine prescription; the traditional Chinese medicine composition has a good application market prospect for treating skin warts and condyloma.

The invention relates to the technical field of molecular biology and biomedicine, in particular to a separation and extraction method and application of extracellular vesicles derived from brucea javanica. The separation and extraction method comprises the following steps: S1, preparing supernate containing active ingredients of brucea javanica; s2, sequentially performing micron-scale filtration and nano-scale filtration on the supernate to obtain an extracellular vesicle concentrated solution; and S3, mixing the extracellular vesicle concentrated solution with a glucosamine sulfate solution, and performing freeze-drying to obtain freeze-dried powder of the extracellular vesicles derived from brucea javanica. The separation and extraction method is simple in operation process, mechanical damage to the vesicle structure caused by a traditional ultracentrifugation method is effectively avoided, and the extracted vesicles are uniform and good in form, uniform and concentrated in particle size distribution, good in stability and high in purity.

The application discloses a preparation method of a special pesticide for aerial spraying containing brucea javanica oil, which comprises the following components in percentage by weight: 0.2-40% of a pesticide active agent, 5-20% of a surfactant, 5-20% of a cosolvent, 0.01-1% of an antioxidant, and the rest of brucea javanica oil. Compared with the prior art, the special pesticide for aerial spraying has remarkable anti-drifting effect and obvious field efficacy.

The invention discloses a brucea javanica extract-cordycepin HPV (human papillomavirus) attenuated preparation as well as a preparation method and application thereof, and particularly relates to the technical field of antiviral preparations and gynecological medical treatment. The buccal tablet is prepared from the following components: 5%-15% of a brucea javanica extract, 0.3%-1.0% of cordycepin, 1%-3% of a surfactant, 2%-5% of a penetration enhancer, 0.5%-2% of a thickening agent, a pH regulator and the balance of purified water, the dosage of the pH regulator is a dosage for stabilizing the pH value of the preparation in a range of 4.0-6.5. Through pharmacological synergy of the brucea javanica extract and the cordycepin, a breakthrough of dual effects of direct virus resistance and active immune regulation is realized, the virus load is rapidly reduced, and the immune microenvironment of HPV persistent infection is fundamentally reversed by enhancing the self-cleaning capability of the body, so that the immune effect of the human body is improved. Through cooperation of double strategies of physical particle size optimization and chemical penetration enhancement, it is ensured that high-activity medicine components can efficiently permeate and be enriched in a focus part, and therefore the exertion speed and the action strength of the medicine effect are remarkably improved.

The utility model provides a kind of sharp condyloma acuminatum traditional chinese medicine external application pollution-preventing patch, it is related to medical technology field, including protective layer, control release layer, drug-loading layer and adhesive layer, the control release layer is below protective layer, the drug-loading layer is below control release layer, the adhesive layer is below drug-loading layer;The drug-loading layer includes grid structure layer, brucea javanica bitter alcohol layer and porous starch microsphere layer, the brucea javanica bitter alcohol layer and porous starch microsphere layer are inside grid structure layer;The utility model adopts multilayer composite structure, treatment main body is drug-loading layer, treat by podophyllotoxin cooperation brucea javanica bitter alcohol layer, brucea javanica bitter alcohol can alleviate symptom and auxiliary remove wart body by the effect of heat-clearing and detoxifying, antiviral and erosion wart, podophyllotoxin is cell division inhibitor, can directly destroy wart body, treatment effect is good, medicine is fixed by grid structure layer, podophyllotoxin is loaded by porous starch microsphere layer, improves the stability of drug loading.

The invention relates to the technical field of essence additives, and particularly discloses an additive for improving the illumination stability of essence and application of the additive. The additive for improving the illumination stability of the essence comprises the following components in parts by mass: 1-5 parts of a light stabilizer; 1.5 to 3 parts of an antioxidant; 3-7 parts of compound vegetable oil; 85 to 94.5 parts of a solvent; the compound vegetable oil is compounded by frankincense oil, carrot seed oil, brucea javanica oil and arnica oil. The method has the advantage that the illumination stability of the essence is further improved.

The invention discloses a nucleic acid delivery carrier based on natural lipids and a preparation method and application thereof, the natural lipids of the nucleic acid delivery carrier comprise coix seed oil, brucea javanica oil and soybean oil, and other components comprise DOTAP, DOPE, glycerin and deionized water. The nucleic acid delivery carrier for delivering the siRNA drug has difference in cellular uptake and transfection efficiencies, and the lysosome escape condition and lipid droplet co-localization result taking the coix seed oil and the brucea javanica oil as lipids are superior to those of soybean oil; and the in-vitro transfection efficiency of the coix seed oil nucleic acid delivery vector on AsPC-1 and human embryo kidney cell strain HEK293T cells has no significant difference with the cell transfection efficiency of a commercially available nucleic acid delivery vector. The constructed natural nucleic acid delivery carrier is prepared into a freeze-dried preparation, so that the stability of the nucleic acid delivery carrier can be improved; the natural nucleic acid delivery carrier which is redissolved after being freeze-dried still keeps relatively good transfection efficiency.

The invention relates to the technical field of traditional Chinese medicine verruca removing, in particular to a traditional Chinese medicine composition for removing verruca as well as a preparation method and application thereof. The traditional Chinese medicine is prepared by mixing the following components in parts by weight: 25-35 parts of radix curcumae, 25-35 parts of rhizoma curcumae, 15-25 parts of brucea javanica powder, 5-15 parts of old salt, 15-25 parts of Indian kalimeris seed, 15-25 parts of malan flower, 45-55 parts of rhizoma cyperi, 45-55 parts of smoked plum, 45-55 parts of horsetail, 10-20 parts of cortex phellodendri, 7-17 parts of radix scutellariae, 1-10 parts of coptis chinensis, 1-8 parts of earthworm extract, 1-5 parts of centipede powder, 1-10 parts of alum, 1-8 parts of sodium bicarbonate, 3-8 parts of borneol and 5-15 parts of pearl powder. According to the invention, unconventional components are introduced, the compatibility relation is reconstructed, and a modern monarch, minister, assistant and guide system with three-order progression and multi-target cooperation is formed by combining the improvement of a production process, so that the verruca nevus can be safely and effectively removed.

The invention provides Zhuang herbal medicine liquid for treating scars and a preparation method, and belongs to the technical field of traditional Chinese medicine. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 25-50 parts of radix notoginseng, 15-25 parts of radix ranunculi ternati, 15-25 parts of gadfly, 5-15 parts of willow bark, 15-25 parts of silkworm larva, 15-25 parts of concha arcae, 5-15 parts of lantana caerulea, 5-15 parts of wikstroemia indica, 5-15 parts of impatiens balsamina, 5-15 parts of wild sophora flavescens, 15-25 parts of Guangxi golden-edge leech, 5-15 parts of selfheal, 15-25 parts of radix taxilli, 5-15 parts of brucea javanica, 5-15 parts of kelp and 5-15 parts of adder tongue herb. The traditional Chinese medicine composition is prepared from 5-15 parts of flos chrysanthemi indici, 5-15 parts of periostracum cicadae, 5-15 parts of flos physalis alkekengi, 5-15 parts of camphor powder and 5-15 parts of plum blossom borneol powder. The pharmaceutical composition is reasonable in formula and exact in curative effect, and has the effects of easing pain, relieving itching, softening hardness, dissipating binds and promoting scar tissue softening, fading, fading and smoothing.

The invention relates to the technical field of plant genetic engineering, and provides a cold-resistant gene BcACL5.2 of black cabbage, and the nucleotide sequence of the cold-resistant gene BcACL5.2 is as shown in SEQ ID NO.1. The invention also provides an overexpression vector containing the gene and a host cell. Meanwhile, the invention also provides an application of the gene BcACL5.2 in breeding for improving the cold tolerance of plants and a method for improving the cold tolerance of the plants. According to the invention, a key gene BcACL5.2 related to the cold resistance is screened from black cabbage, and the gene is over-expressed in an arabidopsis thaliana plant, so that the positive regulation of the gene on the cold resistance of the plant is verified, and the growth ability of the plant under low-temperature stress can be improved; the invention lays a foundation for cultivating a new plant variety with strong cold resistance, especially a new black cabbage variety, and has important value for expanding a black cabbage planting area and promoting industrial development.