The invention discloses a
metal covalent organic framework material, a mediated steady-state electrochemical
luminescence sensor and application of the mediated steady-state electrochemical
luminescence sensor in the field of
escherichia coli detection.The preparation method of the
metal covalent organic framework material comprises the steps that
tris (4, 4 '-
dicarboxylic acid-2, 2'-bipyridyl)
ruthenium dichloride, 1, 2, 4, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, the preparation method comprises the following steps: adding 1, 3, 5-phenylenetetramine tetrahydrochloride and polyphosphoric acid into a
polytetrafluoroethylene autoclave, heating at 150 DEG C for 24 hours, and then heating at 180 DEG C for 24 hours to obtain a mixture; and adjusting the pH value of the obtained mixture to 8.5 by using a saturated NaHCO3 solution, precipitating black
powder, collecting the black
powder by
centrifugation, washing for 12 hours by using a
Soxhlet extractor in sequence by using water,
methanol and
acetone, and carrying out
vacuum drying on the washed black
powder at 100 DEG C for 6 hours to obtain the
ruthenium metal covalent organic framework material. According to the invention, the Ru-MCOFs material is used as a
signal source of an electrochemical sensor, a PEG-CS modified interface is constructed on an
electrode so as to enhance the antifouling property of the sensor in a
complex matrix, and a large amount of Ru-MCOFs material is fixed on the surface of the
electrode by adopting a molecular amplification method (
polymerase chain reaction PCR and HCR), so that the stable electrochemical sensor is developed and assembled; the method can be used for detecting common food-borne
pathogenic bacteria.