22 results about "Insecticide resistance" patented technology

The invention provides an RPA-CRISPR-Cas12a system-based detection reagent for the drug resistance of a chilo suppressalis organophosphorus insecticide, a detection method and application, and belongs to the technical field of molecular detection. The invention provides a detection reagent for chilo suppressalis organophosphorus insecticide resistance, the reagent comprises a specific RPA2 primer pair designed for chilo suppressalis acetylcholin esterase ace-1 gene A314S resistance mutation site and crRNA, a double screening mechanism of RPA amplification and CRISPR-Cas12a is combined, wild type and mutant type genes can be accurately distinguished, and the detection reagent can be used for detecting the insecticide resistance of the chilo suppressalis organophosphorus insecticide resistance to the chilo suppressalis organophosphorus insecticide resistance to the chilo suppressalis organophosphorus insecticide resistance to the chilo suppressalis organophosphorus insecticide resistance to the chilo suppressalis. The rapid, sensitive and visual nucleic acid detection of the chilo suppressalis ace-1 gene A314S resistance mutation site is realized, and a visual detection means which is high in specificity, simple and convenient to operate, economical and efficient is provided for field on-site determination of chilo suppressalis resistance populations.

The invention relates to an insect adenosine triphosphate binding cassette superfamily transport protein and application thereof. The insect adenosine triphosphate binding cassette superfamily transporter is a homodimer, and each monomer has an adenosine triphosphate binding and hydrolysis structural domain, a transmembrane structural domain containing 6 transmembrane helixes and an extracellular structural domain; in a transmembrane structure domain of the protein structure, a spiral 1, a spiral 2 and a spiral 5 of each monomer form an arched substrate binding channel. Experiments show that the adenosine triphosphate binding cassette superfamily transporter and a pesticide micromolecule substrate are bound in the arched substrate binding channel and are discharged out of a body, so that drug resistance is generated, and the transport protein can be used for treating the pesticide micromolecule substrate. Therefore, the insect adenosine triphosphate binding cassette superfamily transport protein can be used as a target of insecticide resistance so as to design an efficient inhibitor compound, and can be used as a target spot for solving the insecticide resistance.

The invention discloses a sanitary insecticidal composition containing cyprosulfuron and propoxur and application thereof, the effective components are cyprosulfuron and propoxur, the total content of the cyprosulfuron and propoxur is 2%-70%, and the balance is an auxiliary agent; the weight ratio of the cyprosulfuron to the propoxur is (15: 1)-(1: 15); the dosage form of the sanitary insecticidal composition is a suspending agent, an oil suspending agent, a dry suspending agent, wettable powder and water dispersible granules. According to the sanitary insecticidal composition, on one hand, the environmental compatibility is good, the use amount of pesticides is reduced, on the other hand, from the aspect of the action mechanism, the action mechanisms of the cyprosulfuron and the propoxur are different, cross resistance does not exist, the control effect is improved, and the insecticide resistance of pests can be delayed by compounding the two insecticides with different action mechanisms; the resistance treatment of the pests is facilitated.

This invention belongs to the field of insecticide technology, specifically relating to a diamide compound, its preparation method, intermediate preparation equipment, and applications. The preparation method includes: reacting 2-fluoro-3-nitrobenzoic acid with thionyl chloride in the presence of dimethylformamide to generate compound A; condensing compound A with 2-trifluoromethyl-4-heptafluoroisopropyl-6-bromoaniline amide to obtain compound B; reducing compound B to obtain compound C; substituting compound C with formaldehyde to obtain compound D; and condensing compound D with 3,5-dichloro-4-fluorobenzoyl chloride to obtain the diamide compound. The beneficial effects of this invention are: the diamide compound solves the problem of insecticide resistance and possesses high insecticidal activity, exhibiting good insecticidal effect at low doses. Furthermore, the preparation method uses mild reaction conditions, produces few byproducts, and has a high reaction yield, reducing raw material loss and thus lowering production costs.

The present invention relates to the technical field of pesticides, and in particular to an active foliar fertilizer with insecticidal efficacy. An insecticidal composition, characterized in that its active ingredient is a binary compound of cyclohexaprid and farnesol. The insecticidal mechanisms of farnesol and other active ingredients in the insecticidal composition of the present invention are different. When used in combination, they can effectively delay the development of insecticide resistance and extend the service life of the insecticidal composition. Secondly, the pest control effect can be improved, the amount of active ingredients used can be reduced, and the cost of pest control can be reduced. In addition, the active foliar fertilizer of the present invention contains both insecticidal ingredients and water-soluble fertilizer ingredients. When used, it can not only prevent and control crop pests, but also provide nutrients for crops. The two cooperate with each other to improve the yield and quality of crops.

The invention belongs to the technical field of pesticide disinfestation, and discloses an insecticidal composition containing a biological source insecticide and application thereof.The active ingredients of the insecticidal composition comprise a compound shown in the formula I and the biological source insecticide, the structure of the compound shown in the formula I is shown in the specification, the biological source insecticide is any one of spinetoram or spinosad, and the structural formula of the compound shown in the formula I is shown in the specification. The mass ratio of the compound shown in the formula I to the biological source insecticide is (1: 30)-(32: 1). The insecticidal composition provided by the invention has an excellent insecticidal effect, has multiple action mechanisms and sites, and can effectively delay the occurrence and development of pest resistance to drugs; cost is low, and economic benefits are remarkable.

The invention discloses a rapid visual detection kit for drug resistance of chilo suppressalis bisamide insecticides and application of the rapid visual detection kit. According to the invention, by utilizing the synergistic effect of RPA and CRISPR / Cas12a systems, Y4667D and I4758M mutation sites closely related to resistance in chilo suppressalis ryanodine receptor genes are specifically targeted, an RPA primer pair and crRNA are provided, a one-pot detection method is established, a reaction can be triggered only by manual uniform mixing at a constant temperature, and a centrifugation step is not needed; a coarsely extracted DNA sample can be directly used, so that a tedious purification process is omitted; in combination with a fluorescently-labeled DNA probe, chilo suppressalis individuals carrying key resistance mutations and sensitive individuals can be quickly identified and distinguished with high sensitivity, and the result can be directly observed and interpreted by naked eyes. A visual detection scheme which is extremely simple and convenient to operate, rapid in reaction, excellent in specificity and economical in cost is provided for monitoring chilo suppressalis bisamide insecticide resistant populations in the field in real time.

The present invention provides a cytochrome CYP6AE28 of Cnaphalocrocis medinalis and its application, and the nucleotide sequence of CYP6AE28 is as shown in SEQ ID NO.1. The CYP6AE28 of the present invention and its encoded protein are of great significance for analyzing the insecticide resistance mechanism of Cnaphalocrocis medinalis, controlling its resistance, and ensuring the green and safe production of rice.

The present invention relates to novel active compound combinations comprising as compound (A) spidoxamat and as compound (B) acetamiprid and as compound (C) at least one further compound selected from Insecticide Group (IRAC (Insecticide Resistance Action Committee) mode of action classification groups; also designated as "IRAC classification groups") (3), which are highly suitable for controlling animal and microbial pests.

This invention belongs to the field of pesticide compound technology, specifically relating to an insecticidal composition. The active ingredients of the insecticidal composition include active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula (Ⅰ), and active ingredient B is an arylisoxazoline insecticide. The mass ratio of active ingredient A to active ingredient B is 1:30 to 40:1. The compound insecticide of this invention has a significant synergistic effect, reduces the amount of insecticide used, lowers production costs and environmental risks, delays the development of insecticide resistance in pests, and can be used for integrated management of resistant pests, specifically for controlling Hemiptera, Lepidoptera, and Coleoptera pests.

The present invention belongs to the field of pesticide control technology and discloses an insecticidal composition containing a biogenic insecticide and its use. The active ingredients of the insecticidal composition include a compound of Formula I and a biogenic insecticide. The compound of Formula I has the following structure: #imgabs0# The biogenic insecticide is either spinetoram or spinosad, and the mass ratio of the compound of Formula I to the biogenic insecticide is 1:30 to 32:1. The insecticidal composition of the present invention has excellent insecticidal efficacy, multiple mechanisms of action and sites, and can effectively delay the development and progression of insecticide resistance in pests. It is also low-cost and has significant economic benefits.

The invention relates to an AgDoa gene and application thereof in reduction of insecticide resistance of cotton aphid, and belongs to the technical field of biological genetic engineering, the AgDoa gene has a DNA sequence as shown in SEQ ID No: 1, and consists of 1782 nucleotides; the amino acid sequence of the protein coded by the AgDoa gene is shown as SEQ ID No: 2, and the protein consists of 593 amino acids; the AgDoa gene can be used as a target to develop a novel insecticide; the AgDoa gene is knocked down, so that the tolerance of the aphis gossypii glover to the insecticide is reduced, and the aphis gossypii glover can be applied to drug resistance treatment and novel pesticide development.

The present application relates to the technical field of pesticides and insecticides, and more particularly to a kind of insecticidal composition containing bromofenac flubendiamide and its application. A kind of insecticidal composition containing bromofenac flubendiamide, by mass fraction, includes the following preparation raw materials: 10-12 parts of insecticide active ingredients, 8-10 parts of emulsifiers, 20-24 parts of penetrants, 0.8-1.2 parts of xanthan gum, 1-3 parts of composite thickeners, 0.2-0.3 parts of antifreeze, 55-60 parts of solvents, wherein the insecticide active ingredients are prepared by mixing bromofenac flubendiamide and emamectin benzoate. The insecticidal composition containing bromofenac flubendiamide provided by the present application has the characteristics of small dosage, low cost and good drug efficacy, which can not only expand the insecticidal spectrum, but also delay pest resistance, and is environmentally friendly, and is a kind of efficient, economical and sustainable agricultural compound insecticide.

The invention belongs to the technical field of pesticide disinfestation, and discloses an insecticidal composition containing an amide insecticide and application thereof.The active ingredient of the insecticidal composition comprises an active ingredient A and an active ingredient B. The active ingredient A is a compound shown in the formula I, and the active ingredient B is the amide insecticide. The amide pesticide is any one of tolfenpyrad, cyhalodiamide, tetrachlorantraniliprole, polyflubendiamide, thiotraniliprole and thiodicyclaniliprole, and the compound pesticide is a compound of the following components in parts by weight; the mass ratio of the active component A to the active component B is (1: 40)-(40: 1). According to the insecticidal composition, the insecticidal effect is greatly improved, the utilization rate of the insecticide is increased, the development of insecticide resistance of pests is slowed down, and the service life of the insecticide is prolonged.

This invention discloses a compound agricultural insecticide containing brofenoxam and its application. The active ingredient of the compound agricultural insecticide composition of this invention is brofenoxam and either 1) or 2) of the following: 1) lufenuron; 2) a cyclophosphamide, wherein the mass ratio of brofenoxam to lufenuron or cyclophosphamide is 1:1 to 50. The insecticide composition of this invention can be formulated as a suspension concentrate, emulsion, dispersible oil suspension, wettable powder, soluble powder, water-dispersible granules, and microemulsion. The compound composition of this invention is used to control Lepidoptera, Coleoptera, and Thysanoptera pests on crops. Compared with single agents, it has a significant synergistic effect, can reduce the dosage, and can delay the development of insecticide resistance in pests, making it particularly suitable for the control of resistant pests.

The invention relates to an identification method and application of a molecular marker for brown planthopper insecticide resistance, in particular to a molecular marker for identifying brown planthopper insecticide resistance by using nucleotide encoding the 414th site of SEQ ID NO: 1, and provides a method for identifying brown planthopper with insecticide resistance. A homozygous brown planthopper population carrying the 414th-site homozygous mutation and a homozygous brown planthopper population not carrying the 414th-site homozygous mutation are separated by utilizing the method, and the method can be used for screening and developing novel insecticides.

To provide combinations of insecticides comprising naturally derived insecticides that reduce health problems in humans and also reduce the risk of developing insecticide resistance.SOLUTION: Provided are pesticidal mixtures comprising sabadilla alkaloids and pyrethrum esters, and methods of controlling pests including insects and mites by application of pesticidal mixtures comprising sabadilla alkaloids and pyrethrum esters.SELECTED DRAWING: None

The invention relates to a primer pair for detecting pyrazol insecticide resistance of brown planthopper and application of the primer pair, in particular to an upstream primer, namely 5 '-GAGTTTACAGCAGGCGGAC-3'; the invention discloses a primer pair of Rp: 5 '-GCAGAATCGCGCTAGAAAGA-3', downstream primers Rp: 5 '-GCAGAATCGCGCTAGAAAGA-3' and Sp: 5 '-GCAGAATCGCGCTAGAAAGG-3', and the primer pair is used for detecting the pesticide resistance of brown planthopper by adopting an allele specific PCR (Polymerase Chain Reaction) method. The primer pair has the characteristics of short detection time, less manpower, less sample amount, large sample detection amount, high accuracy and the like.

The invention discloses a suspension type thrip control medicament, the medicament is in a water suspension dosage form, the components of the medicament comprise cyantraniliprole, chlorfenapyr and an auxiliary agent, and the mass ratio of cyantraniliprole to chlorfenapyr in the medicament is 1: 1-1: 20. The insecticide is used for preventing thrip pests, cyantraniliprole and chlorfenapyr in the insecticide are both effective components, after compounding, the insecticide resistance of pests can be reduced, the insecticide is low in toxicity and residue, the dosage is reduced, the use cost is reduced, environmental pollution is relieved, and the insecticide has the gain effect of compounding and addition.

The present invention relates to novel active compound combinations comprising as compound (A) spidoxamat and as compound (B) ethiprole and as compound (C) at least one further compound selected from Insecticide Group (IRAC (Insecticide Resistance Action Committee) mode of action classification groups; also designated as "IRAC classification groups") (3), which are highly suitable for controlling animal and microbial pests.

The present inventions concerns use of isocycloseram to control mosquitoes including mosquito vectors of pathogenic disease and mosquitoes having developed insecticide resistance, such as pyrethroid insecticide resistance.

This application provides a detection primer, method, and application for the Y4667D / C mutation of the nicotine receptor in the rice stem borer, belonging to the field of agricultural pest resistance detection technology. The detection primer set is designed for the Y4667D / C mutation site, a target receptor for the rice stem borer's high resistance to diamide insecticides. It includes a first primer set for simultaneous detection of Y4667D and Y4667C, a second primer set for genotypic specific detection of Y4667D, and a third primer set for genotypic specific detection of Y4667C; each primer set contains 6-8 primers (outer, inner, and loop primers). The detection method primarily relies on visual interpretation of genotypes through color changes in the reaction solution, eliminating the need for a thermal cycler. It offers advantages such as multiple tests per tube, flexible splitting, and high sensitivity. This invention is suitable for rapid field detection of mutant genotypes and frequencies in the rice stem borer, providing technical support for diamide insecticide resistance monitoring and scientific management.