This application provides a detection primer, method, and application for the Y4667D / C
mutation of the
nicotine receptor in the rice stem borer, belonging to the field of
agricultural pest resistance detection technology. The detection primer set is designed for the Y4667D / C
mutation site, a target
receptor for the rice stem borer's
high resistance to diamide insecticides. It includes a first primer set for simultaneous detection of Y4667D and Y4667C, a second primer set for genotypic
specific detection of Y4667D, and a third primer set for genotypic
specific detection of Y4667C; each primer set contains 6-8 primers (outer, inner, and loop primers). The detection method primarily relies on
visual interpretation of genotypes through
color changes in the reaction solution, eliminating the need for a
thermal cycler. It offers advantages such as multiple tests per tube, flexible splitting, and high sensitivity. This invention is suitable for rapid
field detection of
mutant genotypes and frequencies in the rice stem borer, providing
technical support for diamide
insecticide resistance monitoring and
scientific management.