77 results about "Adenosine triphosphate" patented technology

The invention provides a genetically engineered bacterium for producing O-succinyl-L-homoserine as well as a construction method and application of the genetically engineered bacterium. In a chassis bacterium genome, the expression of a 2-ketoglutaric acid decarboxylase encoding gene sucA is enhanced, and the expression of a succinyl-coenzyme A synthetase encoding gene sucD is weakened, so that the supply of succinyl-coenzyme A is increased; the method comprises the following steps: increasing the NADPH (Nicotinamide Adenine Dinucleotide Phosphate) reducing capacity and ATP (Adenosine Triphosphate) energy supply of a chassis bacterium, increasing DNA (Deoxyribose Nucleic Acid) in combination with a transcription dual regulatory factor ompR to improve the stress resistance of escherichia coli under high osmotic pressure, and introducing an overexpression plasmid containing a homoserine transsuccinylase coding gene metA to construct the genetically engineered bacterium for producing O-succinyl-L-homoserine. The engineering strain obtained through a systematic metabolic engineering modification strategy can realize effective accumulation of OSH, the shake flask yield of OSH reaches 19.8 g / L, the fed-batch fermentation yield of a 5L fermentation tank reaches 110.5 g / L, the sugar-acid conversion rate reaches 52.6%, and a foundation is laid for subsequent construction of high-yield OSH engineering bacteria.

The invention provides a yeast rich in polyphosphate and application of the yeast in synthesis of adenosine triphosphate, and the content of polyphosphate is 15-35 wt% based on the dry matter mass of the yeast rich in polyphosphate; according to the method disclosed by the invention, the yeast is subjected to phosphorus starvation culture and then is subjected to phosphorus supplementary culture, so that the yeast is enriched with polyphosphates, and the yeast rich in polyphosphates is obtained; then, the yeast rich in polyphosphate is used as a biocatalyst to synthesize ATP, that is, self-related enzyme systems, mainly including adenosine kinase, endo-polyphosphate kinase and excision-polyphosphate kinase, in the yeast rich in polyphosphate are used for replacing exogenous polyphosphate kinase and adenosine kinase, and in addition, ATP is synthesized by using the yeast rich in polyphosphate as a biocatalyst. According to the method for synthesizing ATP by using the yeast rich in polyphosphate as the biocatalyst, exogenous adenosine kinase and exogenous polyphosphate kinase do not need to be additionally added, the cost is low, the efficiency of converting adenosine into ATP is high, the batch-to-batch stability of the yeast is realized, and the enzyme activity of the self-related enzyme system is high.

The invention discloses a method for catalytically synthesizing adenosine triphosphate by using microcrystalline cellulose carrier purification immobilized enzyme, which comprises the following steps: preparing bacterial suspension by using escherichia coli wet thalli and Tris-HCL buffer solution, crushing, centrifuging, and taking supernate to obtain crude enzyme; adding a microcrystalline cellulose carrier and the wet thalli into the crude enzyme liquid, and washing the immobilized enzyme after immobilization to obtain the immobilized enzyme; the method comprises the following steps: adding immobilized enzyme adenosine kinase and immobilized polyphosphate kinase into a substrate system with the volume of 1L according to a weight ratio, and starting a catalytic reaction under the conditions that the pH is 5.5-7.5 and the temperature is 20-40 DEG C; after catalysis is finished, the immobilized enzyme adenosine kinase and the immobilized polyphosphate kinase are subjected to solid-liquid separation with reaction liquid through a Buchner funnel, the immobilized enzyme continues to be subjected to catalytic reaction, and the method for preparing the immobilized enzyme is simple in mode, low in cost, easy to industrially produce, high in stability and capable of being repeatedly used for many times, and products are easy to separate.

The invention relates to a biosynthesis method of pyridoxal phosphate or pyridoxine phosphate, and belongs to the technical field of biosynthesis. In order to solve the existing problem of serious environmental pollution, the invention provides a biosynthesis method of pyridoxal phosphate or pyridoxine phosphate, which comprises the following steps: in the presence of polyphosphate, magnesium salt, ATP (adenosine triphosphate), pyridoxal kinase and polyphosphate kinase, performing phosphorylation reaction on pyridoxal or pyridoxine and acid salts thereof, thereby obtaining the pyridoxal phosphate or pyridoxine phosphate. The temperature of the phosphorylation reaction is controlled to be 30-38 DEG C, after the reaction is finished, protein separation is conducted on reaction liquid, mother liquor is separated, the compound pyridoxal phosphate or pyridoxine phosphate shown in the formula I is obtained, and the mother liquor is recycled and reused. According to the method, a large amount of ammonium acetophosphate does not need to be additionally added, operation is simpler, mother liquor can be recycled, a large amount of waste water is prevented from being discharged, environment friendliness is better achieved, and the yield is high.

The invention provides a composition for promoting ATP (adenosine triphosphate) generation and improving kidney energy metabolism by activating an AMPK / PGC-1alpha pathway. The composition is prepared from the following raw materials in parts by weight: 100 to 400 parts of herba cistanche, 50 to 200 parts of radix astragali seu hedysari, 25 to 100 parts of glycine betaine and 150 to 300 parts of alpha-ketoglutaric acid. The invention further provides a preparation method and application of the composition for improving ATP generation and improving kidney energy metabolism by activating the AMPK / PGC-1alpha pathway. Through combined use of cistanche, astragalus membranaceus, betaine and alpha-ketoglutaric acid, a multi-target synergistic mechanism of AMPK activation, metabolic substrate supplementation and epigenetic regulation is formed, the mitochondrial function and ATP generation of kidney cells are comprehensively improved, the limitation of existing single-component or chemical drugs is broken through, a safer and more efficient intervention strategy is provided for kidney energy metabolism disorders, and the clinical application prospect is broad. The invention provides an innovative solution for renal tubular energy disorder, has dual values of metabolic regulation and kidney protection, and conforms to the development trend of natural medicines. The invention belongs to the technical field of food and health care product processing.

The preparation method comprises the following steps: uniformly mixing a polyelectrolyte solution with positive charges and an adenosine triphosphate solution with negative charges to obtain condensed fluid drops; adding dye, biomacromolecules or nano-particles into the condensed body liquid drops to obtain condensed body liquid drops loaded with guest molecules; and incubating the condensate droplets loaded with the guest molecules, adding a sodium silicate solution, and centrifugally cleaning to obtain the condensate microspheres. According to the invention, a biomimetic mineralization method is proposed for the first time, SiO2 is used as a membrane component, condensed liquid drops are converted into solid microspheres with stable mechanical strength, and a new idea is provided for overcoming the stability bottleneck of the condensed liquid drops.

The invention relates to the technical field of biosynthesis, in particular to a biosynthesis process of adenosine triphosphate and equipment thereof. Comprising the following steps: optimizing an adenosine triphosphate synthetase gene sequence, constructing an expression vector, and introducing the expression vector into a yeast host cell; sub-packaging the engineering yeast group in a pore plate for parallel culture, detecting the acid yield and substrate conversion rate data of each micropore, and screening out elite strains; fermenting elite strains, and dynamically feeding materials by an online monitoring and feedback control system; the yeast strain is subjected to precise transformation through codon optimization and gene overexpression driven by a strong promoter; elite strains are quickly identified by adopting high-throughput screening in cooperation with miniaturized parallel fermentation and real-time optical detection; in the fermentation process, a self-control feedback fed-batch technology is introduced, and a culture environment is stabilized in an optimal physiological state through real-time monitoring and dynamic regulation, so that the production potential of engineering bacteria is fully exerted, and the yield of adenosine triphosphate, the substrate conversion rate and the product purity are improved.

The utility model discloses a solid-liquid two-in-one ATP (adenosine triphosphate) fluorescence detection swab which comprises an independently formed push rod body, a swab bracket and a sampling part, the swab bracket is of a tube body structure in which a kit is packaged, and a combined assembly part is formed at the bottom of the swab bracket; the push rod body and the swab support are assembled in a sealing mode through a piston type structure, and the lower end of the push rod body is provided with an unsealing structure aiming at the kit. The sampling part comprises a reaction tube and a sampler, the sampler comprises an independent hollow tube cotton swab and a hollow tube suction head, and the reaction tube, the hollow tube cotton swab and the hollow tube suction head can be combined and assembled through a piston type structure; when the sampler is used for detecting the swab, one of the hollow tube cotton swab and the hollow tube suction head is selected to be assembled. According to the utility model, the detection requirements in different scenes can be met, and the detection efficiency is improved, the detection accuracy is ensured, and the use cost is reduced.

The invention discloses a preparation method and application of a polysulfide compound Poly (S-[VIM-Br]) / ATP (adenosine triphosphate). The preparation method comprises the following steps: by taking industrial by-products sulfur (S8) and 1-vinyl-3-brominated imidazole onium salt (VIM-Br) as raw materials, synthesizing a multi-sulfur polymer through an anti-sulfuration reaction, and then carrying out ball-milling compounding on the multi-sulfur polymer and acidified attapulgite (ATP). The compound shows high-selectivity adsorption on precious metal Au (III) / Ag (I), the adsorption rates on Ag (I) and Au (III) in complex systems such as gold ore waste residues and waste water are as high as 99% and 93% or above, and the adsorption rate on Au (III) is kept at 76.02% after three times of circulation. The cost is reduced by using cheap raw materials and a room-temperature process; aTP modification significantly improves selectivity and stability in a complex environment; the adsorption process is accompanied by precious metal reduction (Au (III)-Au (0)), and resource recovery is facilitated.

This invention belongs to the field of target characterization technology, and provides an application of PANX2 protein and its mutants as bioporins. The bioporins can detect one or more of the following: metal ions, inorganic salts, amino acids, nucleotides, small molecule drugs, small molecule diagnostic reagents, adenosine triphosphate (ATP), and monosaccharides. In this invention, one or more amino acids of the wild-type PANX2 protein are mutated to common amino acids other than the original amino acids. Compared to the wild-type PANX2 protein, the PANX2 protein mutants can be expressed normally, are stable, and show a significantly improved current signal during detection, with a longer amplitude and fewer spikes.

The invention discloses a luciferase mutant capable of efficiently utilizing non-natural nucleoside triphosphate. In the mutant, histidine (H) at the 244 site is mutated into tryptophan (W) on the basis of a starting enzyme Luc146-1H2 (K357E) at which amino acid No.357 is mutated from lysine (K) to glutamic acid (E). Compared with an evolutionary starting enzyme, the mutant Luc-WE not only can more efficiently utilize ATP (adenosine triphosphate) as an energy donor, but also can more efficiently utilize non-natural nucleoside triphosphate as an energy donor to catalyze the oxidation of D-fluorescein, so that a luminescence value higher than that of the evolutionary starting enzyme is generated; for different nucleoside triphosphate energy donors, the luminescence value is 2-40 times that of an evolution starting enzyme. Under the reaction condition of lower nucleoside triphosphate concentration, the luciferase mutant Luc-WE shows higher sensitivity, and has wider application range and practical value.

1. The name of the design product: adenosine triphosphate biological fluorescence detector. 2. The use of the design product: adenosine triphosphate biological fluorescence detector is used to quickly detect the amount of microorganisms and other biological residues in the sample, and to judge the health condition. It is suitable for monitoring the key control points in food and beverage production process, and for real-time sampling monitoring by medical system and health supervision institutions. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective drawing.

The application provides a synthesis method of acetylated deoxyadenosine triphosphate, and particularly discloses that 2-chloro-1,3,2-benzodioxaphosphepin-4-ketone is used in a synthesis step, so that the synthesis of acetyl adenosine triphosphate is more efficient and stable, the conversion rate of the synthesis of acetyl adenosine triphosphate is high, and a plurality of batches of experiments prove that the conversion rate of the synthesis of acetyl adenosine triphosphate is stable and suitable for batch production.

The invention relates to the technical field of preparation of functional nano materials, in particular to an MXene-coated UiO-66-coated PAP nano compound, a preparation method of the MXene-coated UiO-66-coated PAP nano compound, a flame-retardant compound and application of the flame-retardant compound. The nano-composite is obtained by loading UiO-66 onto the surface of MXene by a hydrothermal method to construct an MXene (at) UiO-66 heterostructure, and then loading dopamine hydrochloride doped adenosine triphosphate onto the surface of the MXene (at) UiO-66 through interface self-assembly. The flame-retardant compound is obtained by blending, coating and curing the nano compound, epoxy resin, a curing agent and an intumescent flame-retardant system. Through the physical barrier of MXene, the gas adsorption / catalysis of UiO-66 and the phosphorus-nitrogen synergistic flame-retardant effect of PAP, the flame retardance and smoke suppression of the flame-retardant compound are remarkably improved, the carbon residue amount reaches 39.0%, the temperature of the back face of a steel plate is reduced by 94.6 DEG C, and the flame-retardant compound is suitable for fireproof scenes such as building steel structures.

The present invention provides a composition comprising adenosine or adenosine triphosphate (ATP) as an absorption enhancer in combination with a nutrient such as a nutrient, protein, peptide, vitamin, phytochemical, mineral, fatty acid or amino acid or a drug. The present invention describes methods of administering ATP or adenosine to improve the bioavailability of a nutrient such as a nutrient, protein, peptide, vitamin, phytochemical, mineral, fatty acid or amino acid or a drug.

The invention relates to the technical field of nano medicines, and discloses an ATP / cerium coordination polymer nano regulator as well as a preparation method and application thereof. The invention relates to an ATP / cerium coordination polymer nano regulator. The invention relates to a self-assembly hydrogel which is prepared from raw materials including cerous nitrate (III), adenosine-5 '-disodium triphosphate, phospholipid polyethylene glycol amino, SS31 peptide and a diselenide cross-linking agent through a self-assembly and cross-linking method, can effectively remove ROS (reactive oxygen species) in an inner ear and protect cells from oxidative stress injury, thereby protecting auditory cells and improving the quality of the inner ear. The traditional Chinese medicine composition has the effect of treating hearing loss caused by oxidative stress induced by noise, can be used for treating hearing loss through middle ear cavity local administration, is simple in preparation method and mild in reaction condition, and can realize large-scale production. Cascade catalysis of cerium (III) and cerium (IV), ATP (adenosine triphosphate) energy supply and a nano system of a selenium biological material are utilized, and a novel method is provided for treating noise-induced hearing loss.

This invention discloses a machine learning-enhanced fluorescence detection method for silver nanoclusters containing split nucleic acid aptamers, used for the detection of adenosine triphosphate (ATP) in aquatic products, belonging to the field of analytical chemistry. This detection method uses two split nucleic acid aptamers of ATP as templates to synthesize DNA-AgNCs. In the presence of the target ATP, a conformational change is induced, resulting in a decrease in fluorescence intensity. Quantitative analysis of ATP is achieved by detecting this fluorescence change. To further improve the detection effect, machine learning is introduced to preprocess the fluorescence spectrum. A recursive feature elimination algorithm is used to screen characteristic wavelengths, and a multi-dimensional feature set is constructed using maximum fluorescence intensity, peak area, and full width at half maximum (FWHM). The optimal classification model is obtained through cross-validation optimization. This invention combines aptamer recognition, DNA-AgNCs, and machine learning to construct a detection method where fluorescence dynamically decreases with increasing ATP concentration. It has advantages such as low cost, simple operation, and good biocompatibility, effectively improving detection sensitivity and stability, and has promising application prospects in the qualitative and quantitative analysis of ATP in aquatic products.

The invention belongs to the technical field of enzyme engineering, and particularly relates to a guanylate kinase mutant with improved thermal stability and application and a product thereof. Aiming at the defects that wild guanylate kinase is insufficient in thermal stability and easy to inactivate in reaction, mutants (A24Q, S75P, K146P and L172I) are obtained by site-directed mutagenesis modification based on a wild guanylate kinase sequence. The mutant is replaced by specific amino acid residues, so that the thermal stability is remarkably improved, meanwhile, good catalytic activity is kept, wider temperature conditions can be tolerated, and the effective action time of enzyme is prolonged. The mutant can efficiently catalyze a phosphate group transfer reaction of guanylic acid and adenosine triphosphate, and is suitable for enzymatic synthesis of nucleoside compounds such as guanosine diphosphate. The application of the enzyme source can reduce the use amount and supplement frequency of enzyme preparations, reduce the production cost, improve the product yield and quality, and provide reliable enzyme source support for the industrial efficient production of nucleoside products.

The invention belongs to the field of biological medicine and protein engineering, and particularly relates to a preparation method of a protease cryoelectron microscope sample and a protease structure analysis method. The preparation method comprises the following steps: providing an enzyme solution of target protease, adding an enzyme inhibitor of the target protease, incubating an obtained mixture, and collecting an incubation product supernatant; and sucking the incubation product supernatant to prepare a cryoelectron microscope sample, the target protease comprises serine protease, the target protease is located on a cell membrane, or / and the target protease depends on ATP (adenosine triphosphate). According to the method, the freezing electron microscope sample is prepared after the enzyme inhibitor of the target protease is added into the enzyme liquid of the target protease for incubation, so that the problems of depolymerization of the membrane protease and instability of a protein soluble region can be solved, and a freezing electron microscope structure of the full-length membrane protease can be successfully analyzed in electron microscope observation; and accurate reference is provided for subsequent design of specific targeted drugs.

A method of ligating a ligation product for detecting abasic sites on DNA target sequence is described. The ligation method uses a pyrene probe that ligates with a second probe in the presence of a ligase. A pyrene may comprise 5′-phosphate 1′-pyrene deoxyribonucleotide at the 5′-terminus. The second probe may comprise a terminal 3′-hydroxy, wherein the 3′-hydroxy is ligated with the pyrene probe. The ligase may be T4 DNA Ligase or PBCV-1 as the latter has been found to improve selectivity. The abasic on the DNA target sequence may be naturally occurring or may be a generated abasic that is formed by the addition of an enzyme. Furthermore, selectivity may be increase by the addition of adenosine triphosphate (ATP) or ligating at a temperature above the probe:target sequence thermal disassociation temperature.

The invention relates to the technical field of enzyme activity detection, in particular to a method for detecting the activity of OAS family protease. The OAS family protease activity detection method comprises the following steps: providing OAS protein; the preparation method comprises the following steps: preparing OAS protein, polyinosinic-polycytidylic acid, fluorophore-labeled polyadenylic acid and adenosine triphosphate into a reaction system, and carrying out enzymatic reaction to obtain a reaction solution; and carrying out gel electrophoresis on the reaction liquid, and representing the enzyme activity of the OAS protein according to a gel electrophoresis result. The detection method has the advantages of being high in repeatability, easy and convenient to operate and high in safety, and can be well suitable for enzyme activity characterization and related research of OAS family protein.

The invention belongs to the technical field of biological catalytic synthesis, and particularly relates to a method for synthesizing beta-nicotinamide mononucleotide (NMN) by enzyme catalysis, which comprises the following steps: by taking D-ribose as a starting substrate, and utilizing a three-step enzyme catalysis reaction system which is synergistically completed by an S-methyl-5-thioribose kinase mutant (SMTK), purine nucleotidase (PNP) and nicotinamide ribose kinase (NRK), synthesizing beta-nicotinamide mononucleotide (NMN) by enzyme catalysis. And efficient synthesis of the NMN is realized. In order to further optimize the cost, an ATP (adenosine triphosphate) regeneration system is innovatively introduced in the process, so that the consumption of ATP in the reaction process is effectively reduced. On the basis, by combining an enzyme directed evolution technology, an immobilized enzyme technology and a continuous reactor design, the catalytic reaction efficiency and the product purity level are remarkably improved. The whole process has the characteristics of greenness and environmental protection, the reaction conditions are mild and controllable, the product yield stably reaches more than or equal to 85%, and the requirements of industrial large-scale production are completely met.

The invention provides application of endothelin in oocyte in-vitro maturation and embryo in-vitro culture. New application of endothelin is found, the endothelin can improve the polar body discharge rate of oocytes matured in vitro, promote cytoplasm maturing and improve the normal arrangement proportion of organelles after maturing, certain promotion is provided for improvement of oocyte fertilization, and meanwhile subsequent blastocyst development is promoted. The invention further provides an in-vitro maturation culture solution of the sheep oocytes and an in-vitro maturation method of the sheep oocytes, the in-vitro maturation culture solution contains endothelin, and after the sheep oocytes are treated with the in-vitro maturation culture solution containing the endothelin concentration, the mitochondrial membrane potential level of the oocytes can be remarkably improved, and the survival rate of the sheep oocytes is increased. ROS (reactive oxygen species) in the oocytes is remarkably reduced, and the ATP (adenosine triphosphate) and GSH (glutathione) levels of the oocytes and the normal proportion level of spindles are improved; the maturation rate, the fertilization efficiency, the blastocyst number and the blastocyst rate are remarkably increased, in-vitro maturation of sheep oocytes and in-vitro development of early embryos are promoted, and the production efficiency of the sheep in-vitro embryos is effectively improved.

The invention belongs to the technical field of library construction, and particularly relates to a DNA library construction kit and application thereof. The invention provides a DNA library construction kit, which comprises a reagent combination or kit for linker connection, and the reagent combination or kit for linker connection comprises a connection reaction liquid; the ligation reaction liquid comprises a buffer solution, magnesium ions, a thiol reducing agent, adenosine triphosphate (ATP) and propylene glycol; the ligation reaction liquid does not contain polyethylene glycol (PEG), and propylene glycol is adopted to replace polyethylene glycol, so that the content of PEG in the ligation reaction system is reduced, and the generation of chimera can be reduced.

The invention belongs to the technical field of biosensing and underwater detection, and particularly relates to an underwater adenosine triphosphate detection system and method. According to the system, a nanogold modified screen-printed electrode is used as a sensing platform, based on an electrochemical aptamer sensing principle, an ATP specific nucleic acid aptamer is fixed in an Au-S bond self-assembly mode to form a molecular recognition interface, and when ATP is combined with the aptamer, characteristics such as interface electron transfer resistance are changed, so that the ATP specific nucleic acid aptamer is identified. The high-sensitivity and high-selectivity detection of ATP is realized by detecting the peak current change of the electroactive reporter molecule; meanwhile, an epoxy resin-silicone rubber-silane coupling agent composite packaging system is adopted, a compact protection layer is formed in a non-working area of the electrode through a stepped heating curing process, and the three parts cooperate to ensure the sealing performance and long-term stability in an underwater environment, so that underwater in-situ detection in a real sense is realized; the target object degradation in the sample collection and storage process is avoided. The method has important value in the fields of marine environment monitoring, underwater biological research and the like.

The invention relates to the technical field of biological medicine, in particular to application of raspberry ketone glucoside in preparation of a preparation for preventing and treating monocolonic insect. Experiments and tests show that the raspberry ketone glucoside shows remarkable killing activity on fish monozoiasis, and meanwhile, the raspberry ketone glucoside also keeps good safety on fishes. After short-time immersion bath administration, the raspberry ketone glucoside can weaken the movement of the monoagonism and influence the generation of ATP (adenosine triphosphate) of the monoagonism. The raspberry ketone glucoside disclosed by the invention has a relatively good prevention and control effect on fish mononimus, and more solutions can be provided for disease prevention and control of aquaculture by developing a preparation based on the raspberry ketone glucoside.

The invention belongs to the field of insecticides and acaricides, and relates to a composition containing alanine-isoxazoline compounds and acaricides. The composition contains an active component A and an active component B, wherein the weight part ratio of the active component A to the active component B is (1: 100)-(100: 1); the active component A is selected from a compound I, a compound II or a stereoisomer thereof; the active component B is selected from inhibitors influencing the growth of chitin synthetase 1 (CHS1) mites, mitochondrial adenosine triphosphate (ATP) synthetase inhibitors, mitochondrial electron transfer complex (III) inhibitors, mitochondrial electron transfer complex (I) inhibitors, acetyl-CoA carboxylase inhibitors and mitochondrial electron transfer complex (II) inhibitors; a trifluoroethyl sulfide type acaricide or other types of insecticidal and acaricide; the composition disclosed by the invention has the advantages of obvious synergism, resistance delaying and the like, and can be used for preventing and treating various pests.

The invention discloses a high-stability anti-interference free fatty acid detection kit as well as a preparation method and application thereof, the kit comprises a reagent R1 and a reagent R2, the reagent R1 comprises a phosphate buffer solution, acetyl coenzyme A synthetase, coenzyme A, adenosine triphosphate, 4-aminoantipyrine and a cyclodextrin-metal organic framework nano stabilizer with the concentration of 5-20 mg / mL; and the reagent R2 is prepared from a Tris-HCl buffer solution, acetyl coenzyme A oxidase, peroxidase, N-ethyl-N-(3-sulfopropyl)-3-methylaniline sodium salt and a cyclodextrin-metal organic framework nano stabilizer with the concentration of 2 to 10mg / mL. Through the CD-MOF nano material with the specific aperture, triple breakthrough of improving the enzyme stability, enhancing the interference resistance of a complex sample and optimizing the detection sensitivity is synchronously realized.

The present invention addresses the problem of providing: a composition that increases the amount of intracellular ATP and the amount of intracellular NAD; and a composition that improves motor performance. More specifically, the present invention provides a composition for increasing the amounts of intracellular adenosine triphosphate (ATP) and intracellular nicotinamide adenine dinucleotide (NAD), the composition being obtained by combining (a) and (b): (a) an NAD precursor or a pharmaceutically acceptable salt thereof; and (b) one or more substances selected from the group consisting of uridine and uridine precursors, or one or more pharmaceutically acceptable salts thereof, and / or one or more substances selected from the group consisting of inosine and inosine precursors, or one or more pharmaceutically acceptable salts thereof.　Furthermore, the present invention provides a composition for enhancing muscle strength, the composition being characterized by inclluding a combination of (c) and (d): (c) one or more substances selected from the group consisting of β-alanine and β-alanine precursors, or one or more pharmaceutically acceptable salts thereof; and (d) one or more substances selected from the group consisting of uridine and uridine precursors, or one or more pharmaceutically acceptable salts thereof, and / or one or more substances selected from the group consisting of inosine and inosine precursors, or one or more pharmaceutically acceptable salts thereof.

The invention provides an application of an inhibitor of a targeted FIGNL1-FIRRM-MACIR complex in preparation of a tumor drug and a composition of the inhibitor of the targeted FIGNL1-FIRRM-MACIR complex. According to the research, the MACIR is identified as a bridge-like adaptive subunit of a FIGNL1-FIRRMAAA + ATP (Adenosine Triphosphate) enzyme complex. The MACIR is directly combined with the FIRRM and the DNA to promote the FIGNL1 mediated RAD51 filamentous structure to be unfolded, so that the damage repair of cross-linking (ICLs) between DNA chains induced by aldehydes or platinum drugs is promoted, and the genome stability is maintained. Pharmacological inhibition is carried out on the FIGNL1-FIRRM-MACIR complex by using two inhibitors, namely, 4-{2-[(5-chloro-2-methoxyanilino) carbonyl] anilino}-4-oxobutyric acid and 2-{[5-(2-ethoxyethyl)-4-hydroxy-6-oxo-1, 6-dihydro-2-pyrimidinyl] sulfenyl}-N-(3-methoxyphenyl) acetamide, which are used for the FIGNL1-FIRRM-MACIR complex, so that the FIGNL1-FIRRM-MACIR complex can be prepared by using the two inhibitors. The cytotoxic effect of platinum drugs in ovarian cancer can be obviously enhanced.