156 results about "Adenosine triphosphate" patented technology

The invention relates to the technical field of biology, and provides a probiotic composition for relieving sarcopenia obesity and application of the probiotic composition. The composition comprises at least two of lactobacillus plantarum, lactobacillus rhamnosus and bifidobacterium animalis subsp. Lactis. Wherein the preservation number of the lactobacillus plantarum is CGMCC (China General Microbiological Culture Collection Center) No.25839; the preservation number of the lactobacillus rhamnosus is CGMCC (China General Microbiological Culture Collection Center) No.26968; the preservation number of the animal bifidobacterium subsp. Lactis is CGMCC (China General Microbiological Culture Collection Center) No. 27998. The composition disclosed by the invention can be used for increasing the muscle content of an organism, improving the muscle tissue form, increasing adenosine triphosphate and inhibiting abnormal increase of body weight, so that the composition can be used for effectively preventing and treating sarcopenia obesity.

The invention provides a genetically engineered bacterium for producing O-succinyl-L-homoserine as well as a construction method and application of the genetically engineered bacterium. In a chassis bacterium genome, the expression of a 2-ketoglutaric acid decarboxylase encoding gene sucA is enhanced, and the expression of a succinyl-coenzyme A synthetase encoding gene sucD is weakened, so that the supply of succinyl-coenzyme A is increased; the method comprises the following steps: increasing the NADPH (Nicotinamide Adenine Dinucleotide Phosphate) reducing capacity and ATP (Adenosine Triphosphate) energy supply of a chassis bacterium, increasing DNA (Deoxyribose Nucleic Acid) in combination with a transcription dual regulatory factor ompR to improve the stress resistance of escherichia coli under high osmotic pressure, and introducing an overexpression plasmid containing a homoserine transsuccinylase coding gene metA to construct the genetically engineered bacterium for producing O-succinyl-L-homoserine. The engineering strain obtained through a systematic metabolic engineering modification strategy can realize effective accumulation of OSH, the shake flask yield of OSH reaches 19.8 g / L, the fed-batch fermentation yield of a 5L fermentation tank reaches 110.5 g / L, the sugar-acid conversion rate reaches 52.6%, and a foundation is laid for subsequent construction of high-yield OSH engineering bacteria.

The invention discloses a synthetic method of 2 '-fluoro-2'-deoxynucleosid-5 '-triphosphoric acid and application of the 2'-fluoro-2 '-deoxynucleosid-5'-triphosphoric acid. The synthesis method comprises the following steps: reacting in a system containing adenosine triphosphate, 2 '-fluoro-2'-deoxynucleoside, metal ions and deoxynucleoside kinase to obtain 2 '-fluoro-2'-deoxynucleoside-5 '-phosphoric acid; and reacting in a system containing polyphosphoric acid, 2 '-fluoro-2'-deoxynucleoside-5 '-phosphoric acid, metal ions and polyphosphate kinase to obtain 2'-fluoro-2 '-deoxynucleoside-5'-triphosphoric acid. The obtained 2 '-fluoro-2'-deoxynucleoside-5 '-triphosphoric acid can be used for preparing heterologous nucleic acid. The invention provides an economic and green new way for synthesis of 2 '-fluoro-2'-deoxynucleoside-5 '-triphosphate, solves the problems of complex steps, non-environment-friendly reagents and high cost in the existing chemical synthesis method, and provides a thought for preparation of nucleotide derivatives.

The present invention relates to the field of biotechnology and discloses a probiotic composition for alleviating sarcopenic obesity and its use. The composition comprises at least two of Lactobacillus plantarum, Lactobacillus rhamnosus, and Bifidobacterium animalis subsp. lactis; wherein the Lactobacillus plantarum has a deposit number of CGMCC No. 25839; the Lactobacillus rhamnosus has a deposit number of CGMCC No. 26968; and the Bifidobacterium animalis subsp. lactis has a deposit number of CGMCC No. 27998. The composition of the present invention can increase muscle mass, improve muscle tissue morphology, increase adenosine triphosphate, and inhibit abnormal weight gain, thereby effectively preventing and treating sarcopenic obesity.

2'-deoxycytidine-5'-monophosphate and an enzymatic synthesis method therefor, relating to the technical field of biosynthesis. The synthesis method comprises: constructing a first engineered strain and a second engineered strain; respectively carrying out fermentation culture and protein separation and purification on the first engineered strain and the second engineered strain to obtain high-purity deoxycytidine kinase and high-purity polyphosphate kinase; using 2'-deoxycytidine as a deoxycytidine substrate and adenosine triphosphate as a phosphate donor, cyclically feeding polyphosphate, and keeping the ratio of adenosine triphosphate to polyphosphate to a preset ratio to form an enzymatic reaction system; and performing an enzymatic reaction to obtain 2'-deoxycytidine-5'-monophosphate. High-concentration deoxycytidine monophosphate can be quickly and effectively produced, so that pollution hazards caused by a chemical synthesis method can be solved, and the input amount of adenosine triphosphate as a phosphate donor in a biosynthesis system can also be reduced, thereby reducing production costs.

The invention belongs to the technical field of flame-retardant materials and composite boards, and particularly relates to a halloysite-based intumescent flame-retardant early-warning impregnation liquid as well as a preparation method and application thereof. A halloysite-based intumescent flame retardant prepared from melamine, adenosine triphosphate and halloysite nanotubes and a flame-retardant early-warning steeping liquor obtained by mixing graphene oxide are introduced into delignified wood, and then the flame-retardant densified wood integrating flame retardance, early warning and high mechanical strength is prepared. The flame-retardant wood has the characteristics of efficient flame retardance, intelligent fire early warning, excellent mechanical property and the like, widens the application range of wood and products thereof in the fields of buildings, home furnishing and the like, and has important significance for protecting life and property safety of people.

The invention discloses an ATP (adenosine triphosphate) loaded polyelectrolyte microcapsule and a preparation method thereof. Belongs to the technical field of polyelectrolyte microcapsule preparation. The polyelectrolyte microcapsule is composed of a capsule wall and a core material, wherein the capsule wall is composed of a cationic polyelectrolyte layer and an anionic polyelectrolyte layer which are alternately adsorbed; the core material is ATP molecules and can be loaded on the hollow part of the multi-layer polyelectrolyte capsule and / or the capsule wall of the multi-layer polyelectrolyte capsule. The ATP capsule provided by the invention can effectively protect the stability of ATP molecules, so that the ATP molecules are prevented from being quickly degraded in organisms, the preparation method and process of the ATP capsule are simple, the size of the capsule, the loading rate and the release rate of ATP can be regulated and controlled by changing the size of a template, the variety of the template, the adding concentration of an ATP solution, the number of layers of the capsule and the external environment, and the ATP capsule has a good application prospect. The application range of ATP in disease treatment is greatly expanded, and the use efficiency and safety of ATP are improved.

The invention belongs to the technical field of characterization of target analytes, and provides application of PANX2 protein and a mutant thereof as biopore protein. The bioporous protein is used for detecting one or more of metal ions, inorganic salts, amino acids, nucleotides, small molecule drugs, small molecule diagnostic agents, adenosine triphosphate and monosaccharides. After one or more amino acids of the wild type PANX2 protein are mutated into common amino acids except original amino acids, compared with the wild type PANX2 protein, the PANX2 protein mutant can be normally expressed and is stable in property, a current signal is obviously improved during detection, the up-down amplitude of the current signal is longer, and burrs are fewer.

The invention relates to a dsRNA insecticide for preventing and treating thrips and a preparation method of the dsRNA insecticide. Belongs to the field of biotechnology and agricultural application. The dsRNA insecticide for preventing and treating the thrips is a preparation taking dsRNA as an effective component, and the dsRNA is of a double-stranded RNA structure containing a positive-sense strand and an antisense strand; the nucleotide sequence of the positive-sense strand is as shown in SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 5, SEQ ID NO. 7, SEQ ID NO. 9 or SEQ ID NO. 11. The invention proves that dsACT and dsVATB can cause degradation of messenger RNA (Ribonucleic Acid) of ATP (Adenosine Triphosphate) enzyme coding genes in thrips and reduce the expression level of related genes; laboratory test and field efficacy test results jointly show that after the dsRNA insecticide is sprayed, the growth and development of the thrips are affected, individual death is further caused, and the achievement lays a good theoretical and application foundation for prevention and treatment of the thrips.

The invention relates to a DNA (deoxyribonucleic acid) ligation buffer solution and application thereof, the DNA ligation buffer solution comprises 5-15 mM of divalent metal ions, 0.5-5 mM of DTT (dithiothreitol), 10-15% of polyethylene glycol, 0.5-5 mM of ATP (adenosine triphosphate) and a Tris-HCl buffer solution, and the pH (potential of hydrogen) value of the Tris-HCl buffer solution is 7-9. The DNA ligation buffer solution can significantly improve the ligation reaction efficiency, and increase ligation products in the ligation reaction and the number of molecules capable of being sequenced during library construction.

The invention provides a yeast rich in polyphosphate and application of the yeast in synthesis of adenosine triphosphate, and the content of polyphosphate is 15-35 wt% based on the dry matter mass of the yeast rich in polyphosphate; according to the method disclosed by the invention, the yeast is subjected to phosphorus starvation culture and then is subjected to phosphorus supplementary culture, so that the yeast is enriched with polyphosphates, and the yeast rich in polyphosphates is obtained; then, the yeast rich in polyphosphate is used as a biocatalyst to synthesize ATP, that is, self-related enzyme systems, mainly including adenosine kinase, endo-polyphosphate kinase and excision-polyphosphate kinase, in the yeast rich in polyphosphate are used for replacing exogenous polyphosphate kinase and adenosine kinase, and in addition, ATP is synthesized by using the yeast rich in polyphosphate as a biocatalyst. According to the method for synthesizing ATP by using the yeast rich in polyphosphate as the biocatalyst, exogenous adenosine kinase and exogenous polyphosphate kinase do not need to be additionally added, the cost is low, the efficiency of converting adenosine into ATP is high, the batch-to-batch stability of the yeast is realized, and the enzyme activity of the self-related enzyme system is high.

The present invention provides a composition comprising adenosine or adenosine triphosphate (ATP) acting as an absorption enhancer when combined with a nutritional material(s) such as nutrients, protein, peptides, vitamins, phytochemicals, minerals, fatty acids, or amino acids or a drug. Methods of administering ATP or adenosine to improve the bioavailability of a nutritional material(s) such as nutrients, protein, peptides, vitamins, phytochemicals, minerals, fatty acids, or amino acids or a drug are described.

The invention discloses a method for catalytically synthesizing adenosine triphosphate by using microcrystalline cellulose carrier purification immobilized enzyme, which comprises the following steps: preparing bacterial suspension by using escherichia coli wet thalli and Tris-HCL buffer solution, crushing, centrifuging, and taking supernate to obtain crude enzyme; adding a microcrystalline cellulose carrier and the wet thalli into the crude enzyme liquid, and washing the immobilized enzyme after immobilization to obtain the immobilized enzyme; the method comprises the following steps: adding immobilized enzyme adenosine kinase and immobilized polyphosphate kinase into a substrate system with the volume of 1L according to a weight ratio, and starting a catalytic reaction under the conditions that the pH is 5.5-7.5 and the temperature is 20-40 DEG C; after catalysis is finished, the immobilized enzyme adenosine kinase and the immobilized polyphosphate kinase are subjected to solid-liquid separation with reaction liquid through a Buchner funnel, the immobilized enzyme continues to be subjected to catalytic reaction, and the method for preparing the immobilized enzyme is simple in mode, low in cost, easy to industrially produce, high in stability and capable of being repeatedly used for many times, and products are easy to separate.

The invention relates to a biosynthesis method of pyridoxal phosphate or pyridoxine phosphate, and belongs to the technical field of biosynthesis. In order to solve the existing problem of serious environmental pollution, the invention provides a biosynthesis method of pyridoxal phosphate or pyridoxine phosphate, which comprises the following steps: in the presence of polyphosphate, magnesium salt, ATP (adenosine triphosphate), pyridoxal kinase and polyphosphate kinase, performing phosphorylation reaction on pyridoxal or pyridoxine and acid salts thereof, thereby obtaining the pyridoxal phosphate or pyridoxine phosphate. The temperature of the phosphorylation reaction is controlled to be 30-38 DEG C, after the reaction is finished, protein separation is conducted on reaction liquid, mother liquor is separated, the compound pyridoxal phosphate or pyridoxine phosphate shown in the formula I is obtained, and the mother liquor is recycled and reused. According to the method, a large amount of ammonium acetophosphate does not need to be additionally added, operation is simpler, mother liquor can be recycled, a large amount of waste water is prevented from being discharged, environment friendliness is better achieved, and the yield is high.

The invention provides a stable slow-release antioxidant skin care composition emulsion as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The stable slow-release antioxidant skin care composition emulsion provided by the invention is prepared from the following components in percentage by mass: 1.2 to 2.5 percent of resveratrol-ATP (adenosine triphosphate) nanoparticle compound, 1.0 to 3.0 percent of chitosan microcapsule, 0.8 to 2.3 percent of active component, 7.2 to 14 percent of stable component and the balance of nano bubble water, the active components comprise tea tree oil, sodium hyaluronate and beta-glucan; the stable component comprises glycerol, ethanol and carbomer; the resveratrol-ATP nano-particle compound and the tea tree oil are coated in the chitosan microcapsule. The result of the embodiment shows that the antioxidant skin care composition emulsion has a very good antioxidant effect on dopa molecules, so that the oxidation synthesis of melanin is obviously inhibited, and the antioxidant skin care composition emulsion can promote the growth of cells and is beneficial to eliminating scars.

The invention provides a composition for promoting ATP (adenosine triphosphate) generation and improving kidney energy metabolism by activating an AMPK / PGC-1alpha pathway. The composition is prepared from the following raw materials in parts by weight: 100 to 400 parts of herba cistanche, 50 to 200 parts of radix astragali seu hedysari, 25 to 100 parts of glycine betaine and 150 to 300 parts of alpha-ketoglutaric acid. The invention further provides a preparation method and application of the composition for improving ATP generation and improving kidney energy metabolism by activating the AMPK / PGC-1alpha pathway. Through combined use of cistanche, astragalus membranaceus, betaine and alpha-ketoglutaric acid, a multi-target synergistic mechanism of AMPK activation, metabolic substrate supplementation and epigenetic regulation is formed, the mitochondrial function and ATP generation of kidney cells are comprehensively improved, the limitation of existing single-component or chemical drugs is broken through, a safer and more efficient intervention strategy is provided for kidney energy metabolism disorders, and the clinical application prospect is broad. The invention provides an innovative solution for renal tubular energy disorder, has dual values of metabolic regulation and kidney protection, and conforms to the development trend of natural medicines. The invention belongs to the technical field of food and health care product processing.

The present invention relates to the field of enzyme engineering, and more particularly to enzyme mutants, enzyme compositions or their immobilized enzymes and their applications and methods for preparing L-carnitine. The present invention provides a simple L-carnitine holoenzyme preparation process, the overall process of which utilizes bulk betaine (N,N,N-trimethylglycine) and acetic acid as raw materials. L-carnitine is directly obtained by two-step continuous conversion by carboxyl reductase (CAR) and aldolase (DERA). This route can be completed in one step using a crude enzyme solution, or by utilizing a recyclable immobilized enzyme, and the reduced coenzyme II (NADPH) and adenosine triphosphate (ATP) used in the first step of the reaction can also be recycled using corresponding enzymes to further reduce production costs. Therefore, the patented L-carnitine preparation process has many advantages, such as being fully green, simple in process, inexpensive to produce, and easy to scale up.

The preparation method comprises the following steps: uniformly mixing a polyelectrolyte solution with positive charges and an adenosine triphosphate solution with negative charges to obtain condensed fluid drops; adding dye, biomacromolecules or nano-particles into the condensed body liquid drops to obtain condensed body liquid drops loaded with guest molecules; and incubating the condensate droplets loaded with the guest molecules, adding a sodium silicate solution, and centrifugally cleaning to obtain the condensate microspheres. According to the invention, a biomimetic mineralization method is proposed for the first time, SiO2 is used as a membrane component, condensed liquid drops are converted into solid microspheres with stable mechanical strength, and a new idea is provided for overcoming the stability bottleneck of the condensed liquid drops.

The invention discloses an application of histidine (His) in promoting maturation start and quality formation of tomato fruits. The method comprises the following steps: irrigating tomato plants with an aqueous solution containing His, or injecting picked immature fruits with an MES buffer solution containing His to promote fruit maturation starting and turning red. Experimental results show that when tomato plants or tomato fruits in the in-vitro immature stage are treated with the His aqueous solution or the MES buffer solution with the specific concentration, tomato fruit maturation starting, coloring and softening can be promoted; according to the present invention, the tomato plants are subjected to His treatment, and the tomato plants are treated by using the 3-(2-Thienyl)-L-alanine (TIH) activating agent of the ATP-PRT biosynthesis pathway rate-limiting enzyme (ATP-PRT) and the adenosine triphosphate (ATP) substrate of the ATP-PRT so as to promote the maturation, the color turning and the softening of the fruits; his treatment and ATP treatment of the ATP-PRT substrate can promote in-vitro immature-stage tomato fruit maturation starting, reddening and seed maturation, and the effect of His can be significantly inhibited by an ethylene receptor inhibitor 1-MCP, which indicates that His can promote conversion of ethylene synthesis of immature-stage tomato fruits from a system I to a system II, and plays a role depending on an ethylene signal pathway.

The invention relates to the technical field of biosynthesis, in particular to a biosynthesis process of adenosine triphosphate and equipment thereof. Comprising the following steps: optimizing an adenosine triphosphate synthetase gene sequence, constructing an expression vector, and introducing the expression vector into a yeast host cell; sub-packaging the engineering yeast group in a pore plate for parallel culture, detecting the acid yield and substrate conversion rate data of each micropore, and screening out elite strains; fermenting elite strains, and dynamically feeding materials by an online monitoring and feedback control system; the yeast strain is subjected to precise transformation through codon optimization and gene overexpression driven by a strong promoter; elite strains are quickly identified by adopting high-throughput screening in cooperation with miniaturized parallel fermentation and real-time optical detection; in the fermentation process, a self-control feedback fed-batch technology is introduced, and a culture environment is stabilized in an optimal physiological state through real-time monitoring and dynamic regulation, so that the production potential of engineering bacteria is fully exerted, and the yield of adenosine triphosphate, the substrate conversion rate and the product purity are improved.

The utility model discloses a solid-liquid two-in-one ATP (adenosine triphosphate) fluorescence detection swab which comprises an independently formed push rod body, a swab bracket and a sampling part, the swab bracket is of a tube body structure in which a kit is packaged, and a combined assembly part is formed at the bottom of the swab bracket; the push rod body and the swab support are assembled in a sealing mode through a piston type structure, and the lower end of the push rod body is provided with an unsealing structure aiming at the kit. The sampling part comprises a reaction tube and a sampler, the sampler comprises an independent hollow tube cotton swab and a hollow tube suction head, and the reaction tube, the hollow tube cotton swab and the hollow tube suction head can be combined and assembled through a piston type structure; when the sampler is used for detecting the swab, one of the hollow tube cotton swab and the hollow tube suction head is selected to be assembled. According to the utility model, the detection requirements in different scenes can be met, and the detection efficiency is improved, the detection accuracy is ensured, and the use cost is reduced.

The invention discloses a preparation method and application of a polysulfide compound Poly (S-[VIM-Br]) / ATP (adenosine triphosphate). The preparation method comprises the following steps: by taking industrial by-products sulfur (S8) and 1-vinyl-3-brominated imidazole onium salt (VIM-Br) as raw materials, synthesizing a multi-sulfur polymer through an anti-sulfuration reaction, and then carrying out ball-milling compounding on the multi-sulfur polymer and acidified attapulgite (ATP). The compound shows high-selectivity adsorption on precious metal Au (III) / Ag (I), the adsorption rates on Ag (I) and Au (III) in complex systems such as gold ore waste residues and waste water are as high as 99% and 93% or above, and the adsorption rate on Au (III) is kept at 76.02% after three times of circulation. The cost is reduced by using cheap raw materials and a room-temperature process; aTP modification significantly improves selectivity and stability in a complex environment; the adsorption process is accompanied by precious metal reduction (Au (III)-Au (0)), and resource recovery is facilitated.

This invention belongs to the field of target characterization technology, and provides an application of PANX2 protein and its mutants as bioporins. The bioporins can detect one or more of the following: metal ions, inorganic salts, amino acids, nucleotides, small molecule drugs, small molecule diagnostic reagents, adenosine triphosphate (ATP), and monosaccharides. In this invention, one or more amino acids of the wild-type PANX2 protein are mutated to common amino acids other than the original amino acids. Compared to the wild-type PANX2 protein, the PANX2 protein mutants can be expressed normally, are stable, and show a significantly improved current signal during detection, with a longer amplitude and fewer spikes.

The invention discloses a method for quantitatively detecting viable and non-culturable bacteria in a respiratory tract of a host and application. According to the method, a respiratory tract bacterium sample is treated by utilizing a designed specific primer in combination with a PMA dye and a real-time fluorescent quantitative PCR technology, and specific bacteria in a living and non-culturable state in the respiratory tract of a host can be accurately and quantitatively detected by the method. Compared with a flow cytometry and a method for detecting living non-culturable bacteria by ATP (adenosine triphosphate), the method disclosed by the invention has the advantages that a certain target bacterium in a complex bacterium sample which is extracted in a host body and is low in concentration and interfered by other microbial components can be accurately quantified, and the experiment accuracy and repeatability are high; and the defect that the traditional detection method cannot distinguish the detection of the viable and non-culturable bacteria of the single bacteria can be overcome. The method provided by the invention can realize rapid and accurate detection of a plurality of viable and non-culturable bacteria in a clinical sample, and can be applied to human health risk assessment.

The application provides a preparation method of a MOF material-based SERS substrate and application thereof, and belongs to the technical field of SERS substrate preparation. The MOF material of ZIF-8 is prepared by using a hydrothermal method, then AuNPs cores are coated in the ZIF-8 shell by using an electrostatic assembly method, and a core-shell material Au@ZIF-8 is prepared as a SERS substrate. The method for preparing the SERS substrate is simple and effective, and interference of reagents except water phase on SERS detection target small molecules is avoided. The MOF surface of the MOF material-based SERS substrate prepared by the application has uniform and adjustable size pore structures, can selectively allow only the to-be-detected small molecules to pass, and realizes detection of adenosine triphosphate in urine.

The invention discloses a luciferase mutant capable of efficiently utilizing non-natural nucleoside triphosphate. In the mutant, histidine (H) at the 244 site is mutated into tryptophan (W) on the basis of a starting enzyme Luc146-1H2 (K357E) at which amino acid No.357 is mutated from lysine (K) to glutamic acid (E). Compared with an evolutionary starting enzyme, the mutant Luc-WE not only can more efficiently utilize ATP (adenosine triphosphate) as an energy donor, but also can more efficiently utilize non-natural nucleoside triphosphate as an energy donor to catalyze the oxidation of D-fluorescein, so that a luminescence value higher than that of the evolutionary starting enzyme is generated; for different nucleoside triphosphate energy donors, the luminescence value is 2-40 times that of an evolution starting enzyme. Under the reaction condition of lower nucleoside triphosphate concentration, the luciferase mutant Luc-WE shows higher sensitivity, and has wider application range and practical value.

1. The name of the design product: adenosine triphosphate biological fluorescence detector. 2. The use of the design product: adenosine triphosphate biological fluorescence detector is used to quickly detect the amount of microorganisms and other biological residues in the sample, and to judge the health condition. It is suitable for monitoring the key control points in food and beverage production process, and for real-time sampling monitoring by medical system and health supervision institutions. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective drawing.

The present invention relates to a method and system for producing adenosine triphosphate (ATP) through an acid / aldehyde-ATP (AAA) cycle in an electrochemical cell.

The application provides a synthesis method of acetylated deoxyadenosine triphosphate, and particularly discloses that 2-chloro-1,3,2-benzodioxaphosphepin-4-ketone is used in a synthesis step, so that the synthesis of acetyl adenosine triphosphate is more efficient and stable, the conversion rate of the synthesis of acetyl adenosine triphosphate is high, and a plurality of batches of experiments prove that the conversion rate of the synthesis of acetyl adenosine triphosphate is stable and suitable for batch production.

The present invention relates to the field of bioengineering, and particularly to mutant compositions, fusion enzymes and methods for preparing collagen tripeptides. The present invention provides mutant compositions, including Mutant 1 and Mutant 2; Mutant 1 has an amino acid sequence as shown in SEQ ID NO: 1 and Mutant 2 has an amino acid sequence as shown in SEQ ID NO: 2. The preparation method provided by the present invention uses inexpensive amino acids as raw materials, and is highly efficiently converted into corresponding dipeptide or tripeptide products under the action of adenosine triphosphate (ATP) and the corresponding ligase. Introducing an ATP recycling system into the reaction system can greatly reduce its usage amount; at the same time, the amount of enzyme used in the actual production process is reduced by constructing fusion enzymes; the reaction and purification processes are further optimized by using immobilized enzyme catalysis, and the preparation scheme is very concise, with high yield, good product quality and easy to scale up.