672 results about "Adenosine triphosphate" patented technology

A peritoneal dialysate containing adenosine triphosphate or a salt thereof, and a peritoneal dialysis method using the dialysate. The peritoneal dialysate is safe and causes less peritoneal injuries even when employed in peritoneal dialysis over a long period of time.

The present invention relates to the use of creatine compound and neuroprotective combinations including creatine, creatine phosphate or analogs of creatine, such as cyclocreatine, for treating diseases of the nervous system. Creatine compounds in combination with neuroprotective agents can be used as therapeutically effective compositions against a variety of diseases of the nervous system such as diabetic and toxic neuropathies, peripheral nervous system diseases, Alzheimer disease, Parkinson's disease, stroke, Huntington's disease, amyotropic lateral sclerosis, motor neuron disease, traumatic nerve injury, multiple sclerosis, dysmyelination and demyelination disorders, and mitochondrial diseases. The creatine compounds which can be used in the present method include (1) creatine, creatine phosphate and analogs of these compounds which can act as substrates or substrate analogs for creatine kinase; (2) bisubstrate inhibitors of creatine kinase comprising covalently linked structural analogs of adenosine triphosphate (ATP) and creatine; (3) creatine analogs which can act as reversible or irreversible inhibitors of creatine kinase; and (4) N-phosphorocreatine analogs bearing non-transferable moieties which mimic the N-phosphoryl group.

A total nutrient liquid fertilizer containing amino acids comprises, by weights, 6-12 parts of N, 1-6 parts of P, 3-10 parts of K, 1-3 parts of Ca, 1-3 parts of Mg, 9-13 parts of Zn, 2-10 parts of Mn, 1-2 parts of B, 0.5-2 parts of Cu, 0.2-0.5 parts of Mo, 3-10 parts of Fe, 10-15 parts of amino acids, 1-10 parts of a plant growth regulator, 2-5 parts of an organic chelating agent and 30-40 parts of water. The liquid fertilizer provides comprehensive nutrition for plants, enhances the freeze, drought, salt and disease resistance of the plants, enhances the synthesis and translocation of substances in the plants, promotes the formation of adenosine triphosphate (ATP), promotes the photosynthesis and protein synthesis, induces the formation of chlorophyll, promotes the cell protoplasm flow, improves the cell vitality and accelerates the growth of the plants.

The present invention is directed to a method of screening for a therapeutic agent useful for treating pulmonary hypertension comprising: contacting an erythrocyte with a candidate therapeutic agent; and detecting a presence or absence of erythrocyte-derived adenosine triphosphate, wherein a greater erythrocyte-derived adenosine triphosphate level indicates the candidate therapeutic agent has greater activity in treating pulmonary hypertension. Additionally, the present invention is directed to methods of treating pulmonary arterial hypertension by stimulating ATP release from erythrocytes through co-administration to a subject in need thereof an amount of a PDE5 inhibitor compound, and an amount of a prostacyclin compound.

A method for treating obesity by activating non-shivering thermogenesis in mitochondria of targeted brown adipose tissue. A photobiomodulation device including one or more light sources is provided. The mitochondria of the targeted brown adipose tissue is photobiomodulated by exposure to light produced by the light source at a wavelength that modulates photoactivity of an electron transport chain and increases proton transfer across a membrane of the mitochondria of the targeted brown adipose tissue towards an intern space of the mitochondria, without increasing production of adenosine triphosphate.

The invention discloses a method for regenerating ATP by enzymatic method, which comprises the following steps: (1) preparing and obtaining ATP regenerating enzyme; (2) regenerating ATP in reaction liquid; (3) separating products and ATP regenerating enzyme. According to the method for regenerating ATP of the present invention, it has the following beneficial effects: (1) adopt three kinds of ATP regenerating enzymes of novel Ppk, Adk and Pap, the ATP consumed in the enzymatic reaction can be recycled and regenerated, greatly reducing the amount of ATP used, regenerated The process is simple and efficient, the reaction is easy to control, and the stability is high. (2) The substrate polyphosphoric acid or its salt to be added to the reaction is cheap and less polluting. (3) The enzymatic reaction does not need to add expensive ATP to start the reaction, add ADP or cheap AMP. (4) ATP regenerating enzyme recovery system was established, which is suitable for large-scale industrial production.

Disclosed herein are methods and devices for stimulating an immune response to a disease in a subject, which involves passing sub-microsecond long pulses of electric fields having an amplitude between 5 kV / cm and 68 kV / cm through an abnormal growth of a subject sufficient to suppress myeloid-derived suppressor cell (MDSC) or regulatory T cell (Treg) production, increase adenosine triphosphate (ATP) or high mobility group box 1 (HMGB1) production, or stimulate dendritic cell activation in the subject.

The invention relates to a preparation method of nicotinamide adenine dinucleotide, which comprises the following steps of: with 1,2,3,5-tetraacetyl-beta-D-ribofuranose as a starting raw material, preparing nicotinamide mononucleotide through sequentially a condensation reaction, an ammonolysis reaction and a phosphorylation reaction; and then mixing the prepared nicotinamide mononucleotide with adenosine triphosphate, generating an enzymic catalytic reaction in the presence of nicotinamide adenosine nucleotide transferase and pyrophosphatase to prepare the nicotinamide adenine dinucleotide. Compared with the prior art, the technical scheme provided by the invention has the advantage that the whole process is simplified and optimized by combination of a chemical method and an enzymic method.

The invention discloses a sensor preparation method based on an ECL-RET action between GO and GQDs and an application on kinas detection, and belongs to the field of electrochemiluminescence (ECL). The preparation method comprises the following steps: coating chitosan on the surface of an electrode, and orderly assembling graphene quantum dots and polypeptides onto the surface of the electrode through a covalent interaction. Under the actions of protein kinase and triphosadenine, the polypeptides carry out phosphorylation reactions, through the specific recognition action between an antibody and an antigen, oxidized graphene conjugated with a phosphorylated antibody is assembled to the phosphorylated serine sites of the polypeptide, thus the distance between the oxidized graphene and the graphene quantum dots is narrowed down, so that the electrochemiluminescence (ECL) of graphene quantum dots is quenched. The larger the concentration of protein kinase is, the more phsophorylated sites are generated on the polypeptide modified electrode surface, the more oxidized graphene is assembled on a sensing interface, the stronger the electrochemiluminescence quenching effect of graphene quantum dots will be, and thus the high sensitive detection on protein kinase is achieved.

The invention relates to a method for determining the content of blood ammonia, and also the reagent kit for blood ammonia diagnosis. The reagent kit comprises cushioning solution, adenosine triphosphate, phosphoenolpyruvate phosphatase, deacidized type coenzyme, ammonia kinase, pyruvate kinase, lactate dehydrogenase, and stabilizer. By mixing sample and reagent of a predetermiend volumetric ratio, generating coupling reaction between them, subjecting the final reactant to biochemiscal analyser, the main wavelength absorbancy variance ratio (speed) can be detected, and the blood ammonia content can thus be measured. The method of the invention can be used to obtain the needed measurement result purely through biochemical analytic instruments, and advantages of the method include higher sensibility, better accuracy, less susceptibility to contamination of internal or external materials, and easy application.

The invention provides a metal organic framework Uio-66-based photoelectric sensor for detecting the activity of protein kinase. The photoelectric sensor constructed through replacing a precious metal by a metal organic framework is provided for the first time, and comprises a conductive electrode coupled with a polypeptide, and a metal organic framework arranged on the conductive electrode, wherein the metal organic framework is loaded with a photoresponse substance; the metal framework can be specifically bound to a phosphorylated polypeptide; and the polypeptide undergoes a phosphorylation reaction in the presence of the activity of a biological enzyme to be detected and adenosine triphosphate (ATP). Photoelectric current changes with the activity of the protein kinase in visible lights, so super-sensitive and highly-accurate detection of the activity of the kinase is realized. The sensor has very high sensitivity; and enzyme inhibition experiments show that the sensor realizes efficient and sensitive detection of the activity of the kinase PKA, and meets use requirements under extremely severe conditions.

The invention discloses a mesenchymal stem cell frozen stock solution which does not contain animal proteins and DMSO (dimethyl sulfoxide). The frozen stock solution comprises trehaloses, a carbohydrate and electrolyte injection, ATP (adenosine triphosphate) and human serum albumins. The frozen stock solution has the advantages of high recovery rate (90%), no influence on characteristics of stem cells, unchanged cell proliferation, unchanged cell differentiation, easy use, good stability and the like. The invention also discloses a preparation method of the frozen stock solution.

The invention relates to the field of biology, in particular to MSC (mesenchymal stem cell) injection as well as a preparation and an application thereof. The injection comprises MSCs and a cell cryopreservation solution, wherein the cryopreservation solution comprises components in percentage by volume as follows: 25%-70% of an electrolyte balance solution, 5%-20% of clinical-grade DMSO (dimethyl sulfoxide), 1%-50% of 20% human serum albumin, 1%-10% of hydroxyethyl starch 130 / 0.4 and 5%-20% of triphosadenine-disodium magnesium chloride freeze-drying powder. The injection is free of animal serum, has clear ingredients and good cell cryopreservation effect and is safe and controllable, long-term storage and long-distance transport are facilitated, the survival rate of cells after recovery is higher than 95%, the vitality is high, and the injection can effectively relieve injury and inflammation symptoms of lesion tissue of lungs and promote tissue regeneration of the lungs, so that acute lung injury can be fundamentally and comprehensively treated.

The invention relates to the encapsulation of luminescence-related molecules, including but not limited to, adenosine triphosphate (ATP), adenylate kinase (AK), alkaline phosphatase (ALP), luminol and luciferin / luciferase cocktails, within liposomes. These liposomes can be employed to enhance the luminescence detection of microorganisms and compounds in various products and samples. The liposomes containing the luminescence-related molecules can bear a probe which has a specific sequence or structure that, in turn can be used to hybridize to, or couple with, a portion of the target analyte. Within the same assay, paramagnetic beads can bear a probe having a specific sequence or structure that, can hybridize to, or couple with, a second portion of the target analyte to create a complex of analyte bound to paramagnetic beads and liposomes. This type of assay can be often referred to as a ‘sandwich’ assay. Once the probes hybridize to, or couple with, their targets, a complex can be formed of the paramagnetic beads, the analyte, or portion thereof, and the liposomes. This complex can then be washed to remove those components that are non-hybridized or non-coupled. Then, the paramagnetic bead-analyte-liposome complexes can be isolated from the sample using magnetic separation techniques and can be treated so as to release their encapsulated ATP, AK or other luminescence-related compounds. The resulting luminescence can then be determined in a chemical assay. This determination can be qualitative (i.e., an absence / presence assay) or quantitative (i.e., which can measure a specific amount of analyte present). Through the use of a cocktail of probe types, the assay can also qualitatively or quantitatively measure the presence of more than one analyte simultaneously. This type of assay can be of commercial importance in clinical and forensic applications, the personal care, pharmaceutical, food and beverage markets, as well as in environmental sample assays.

The invention provides a method for specific detection of staphylococcus aureus. The method is characterized in that a nucleic acid aptamer is modified on the surfaces of magnetic nano particles, quickly recognizes, and is combined with staphylococcus aureus in a solution sample; target fungi combined with the magnetic nano particles are separated and enriched by using a magnetic field; captured fungi are suspended in a lysate with a certain volume; ATP (Adenosine Triphosphate) extracting staphylococcus aureus is released and used; and as the amount of ATP is directly proportional to the concentration of fungi liquid, after a linearity curve of the fungi concentration and the luminous intensity is obtained by an ATP bioluminescence method, staphylococcus aureus can be detected quantitatively. The method has the advantages that the aptamer is easy to synthesize, modify and fix, and can be kept for a long time; by performing combination, separation, enrichment and concentration on the target fungi, sample matrices and other interference fungi are removed effectively; and the detection sensitivity is improved.

A reagent system comprises a first reagent which includes a high pH phosphate buffer, and a second reagent which includes luciferase, luciferin, a magnesium salt and an enzyme stabilizer. The second reagent has a low pH and a buffer with a pK which is near the optimum pH for activity of luciferase. The reagent system may be used in a process for measuring total adenosine triphosphate (ATP) and / or dissolved extracellular ATP, in a fluid containing microorganisms. The reagent system may also be used in a microbiological remediation or production process.

The invention relates to a method for producing glutathione based on enzymic method in vitro. Through expression and catalysis of glutathione compound difunctional enzymes and polyphosphoric acid kinases, tetraphosphate and hexametaphosphate serve as phosphoric acid donors to regenerate ATP (adenosine triphosphate), and the glutathione is generated under the catalytic action of the glutathione compound difunctional enzymes by taking glutamic acid, glycine and cysteine as substrates. Compared with existing glutathione production methods, the method has the advantages that inhibition effect of the glutathione compound difunctional enzymes is low, and the content of the glutathione obtained from a reaction system is high; sodium and kali salt of phosphoric acid donors, such as the tetraphosphate and the hexametaphosphate, used in the reaction process is low in cost and easy to get; the phosphoric acid donors serve as substrates of the polyphosphoric acid to generate the ATP, and accordingly, production cost is reduced greatly; the amino acid ratio of Gly:Gys:Gly is lowered to 2:1:2, the content of amino acid in the system is low, and later product separation is facilitated.

The invention discloses a graphene field-effect transistor biosensor as well as a manufacturing method and a detecting method thereof. The graphene field-effect transistor biosensor comprises glass substrates, wherein the two sides of each glass substrate are respectively provided with an ITO (indium tin oxide); parts of glass substrates at the same side as well as the ITOs of the parts of glass substrates are covered with graphene; the ITOs at the two sides of each glass substrate without graphene are respectively a source electrode and a drain electrode; a PET (polyethylene glycol terephthalate) gasket is covered on each ITO covered with the graphene and is covered with a PET substrate on which a gold film is sputtered; a sample cell is arranged in the middle of each glass substrate by insulating silica gel; the gold film is taken as a grid electrode. The contact resistance is reduced by means of covering the ITOs with graphene, the plane gold film electrode is taken as the grid electrode to exert a uniform electric field and increase the action area of electrolyte and graphene, and by the two aspects, the detection sensitivity is improved and the detection range is enlarged; by detection, the lower limit of adenosine triphosphate reaches 10pM.

The invention relates to a preparation method and application of a core-shell type ultra-micro electrode. The core-shell structure ultra-micro electrode is obtained by preparing ultra-micro electrode fibers of a coaxial structure in a one-step mode through a coaxial electrostatic spinning method and then performing packaging. The coaxial structure comprises a core electrode base layer and one or two surface sensing layers. By the adoption of the one-step forming technology, the complex surface modification process of traditional ultra-micro electrodes (such as glassy carbon electrodes obtained after ablation treatment) is omitted, and the ultra-micro electrode with the diameter ranging from 40 nm to 6 micrometers can be prepared; due to the subsequent treatment temperature ranging from 70 DEG C to 150 DEG C, the structures and functions of organic functional components (such as biological protein and enzymes) in a modification layer of the ultra-micro electrode can be easily maintained, which is of great significance for the pluralistic design of the ultra-micro electrode. The ultra-micro electrode is used for detecting adenosine triphosphate (ATP), dopamine and adrenal hormones and is high in response speed and sensitivity, strong in anti-jamming capability and especially suitable for on-line rapid determination, particularly, real-time non-destructive determination of living cells.

The invention discloses an isothermal nucleic acid amplification reaction reagent which comprises Tris buffer solutions, potassium acetate, magnesium acetate, dithiothreitol, polyethylene glycol, adenosine triphosphate (ATP), deoxy-ribonucleoside triphosphate (dNTPs), phosphocreatine, creatine kinase, a primer group, single-strand binding (SSD) protein, colon bacillus helicase RecQ protein, UvsY protein and DNA polymerase. The invention further discloses an isothermal nucleic acid amplification method which includes steps of extracting DNA or performing inverse transcription after DNA extracting, and adding the isothermal nucleic acid amplification reaction reagent and reacting the mixture at a temperature in a range between 25 DEG C and 45 DEG C for 10 minutes to 60 minutes to complete the nucleic acid amplification. Compared with traditional polymerase chain reaction (PCR) technologies and according to the technology, nearly no professional instrument is needed, the operation is simple, technical requirements for operators are low, and the reaction time only needs about half an hour.

A medical infusion system which increases adenosine triphosphate from carbohydrates by a pump delivering precisely timed and calculated boluses of hormones such as insulin resulting in oscillations of hormones in whole blood sufficient to cover a carbohydrate load equal to no less than forty percent (40%) of the minimum daily allowance for carbohydrates where the blood glucose is from 60 mg / dl to 300 mg / dl. The system comprises a pump and optimally a cassette or cartridge where the plunger rotates as it advances in reference to the cartridge to provide additional accuracy and overcome the forces of inertia and slip-stick as well as eliminate backlash. The system can also use an encoded area and an opening for connection to an infusion tube with an in-line sensor area where sampling probes can be located to optimized adenosine triphosphate production from the mitochondria in the cells.

The invention discloses a method of preparing S-adenosyl methionine, according to the method, by using polyhydroxyl organic reagent to keep the activity of enzyme, by taking adenosine triphosphate precursor, L-methionine and phosphate ions as a substrate and taking glucose and / or Maltose as an energy supplier, and by adding the combination of metal ions, the producing strain with permeability can be used for producing S-adenosyl methionine in the way of whole-cell biocatalysis. The invention can use the combination of metal ions to regulate the metabolism flow to improve energy self-coupling efficiency; and organic reagent is added to keep the activities of thalli and enzyme. The strain with permeability is used for producing S-adenosyl methionine, thereby shortening the synthesis time and accumulating outcomes outside cells, so that the cost of the following separation can be reduced, and the operational steps also can be simplified.

The invention discloses a preparation method of a rare-earth coordination polymer fluorescence probe and an application of the rare-earth coordination polymer fluorescence probe in H2O2 and glucose detection and belongs to the technical field of optical sensing. The preparation method comprises steps as follows: triphosadenine is mixed with a Tris-HCl buffer solution containing Ce<3+>, and the rare-earth coordination polymer fluorescence probe ATP-Ce-Tris is prepared; when H2O2 is present in the solution, Ce<3+> in the ATP-Ce-Tris is oxidized into Ce<4+> by H2O2 to result in fluorescence quenching of the ATP-Ce-Tris, and accordingly, detection of H2O2 is realized; simple and sensitive detection of glucose is further realized through fluorescence quenching of the ATP-Ce-Tris due to H2O2 which is produced through catalytic oxidation of glucose by glucose oxidases.

The invention discloses a method for preparing adenosine triphosphate (ATP) with an immobilized enzyme method. The method comprises the following steps: (1) fixing an ATP production enzyme on an immobilized carrier to prepare an immobilized ATP production enzyme; (2) catalyzing by using the immobilized ATP production enzyme to prepare an adenosine triphosphate reaction solution; (3) separating the product adenosine triphosphate. According to the method disclosed by the invention, three types of novel ATP production enzymes including Ppk, Adk and Pap are adopted, and the ATP can be synthesized through only two steps of enzymatic reaction; a reaction process is simpler, a reaction is more easily controlled and the product quality is more stable; the ATP is prepared by adopting an immobilized enzyme catalysis method, and an immobilized enzyme can he continuously and repeatedly used for a plurality of times, so that the production cost is greatly reduced. Meanwhile, impurities including a lot of proteins, pigments and the like, which are introduced by utilizing yeasts, are avoided, and the product is more easily purified; an intermittent stirring reaction and enzyme reaction column continuous reaction system which is applicable to large-scale production of the ATP is established.

The invention provides preserving fluid of hepatic cells for a biological artificial liver and a preparation method thereof. The preserving fluid is a solution compounded by ultrapure water. The solution contains the following components within the concentration range: 15-25mmol / L of disodium hydrogen phosphate, 1-10mmol / L of sodium hydrogen phosphate dehydrate, 4-6mmol / L of potassium citrate monohydrate, 10-30mmol / L of sodium chloride, 5-10mmol / L of magnesium chloride hexahydrate, 3-10mmol / L of disodium adenosine triphosphate, 1-5mmol / L of reducing glutathione, 0.1-0.5mmol / L of alpha-lipoic acid, 100-150mmol / L of trehalose (C6H12O5), 200 / 0.510-50g / L of hydroxyethyl starch and 2-10mg / L of matrine. The preparation method of the preserving fluid comprises the following steps of: accurately weighing all components according to the concentration requirements of the components, wherein the alpha-lipoic acid is weighed in a dark place; completely dissolving the other components except the alpha-lipoic acid by using the right amount of ultrapure water; sufficiently dissolving the alpha-lipoic acid in the dark place; and adding the ultrapure water to full dose. The preserving fluid can well protect the cell activity of the hepatic cells for the biological artificial liver and the special functions of the hepatic cells at low temperature so as to satisfy the short-term low temperature preservation of a large-scale hepatic cell bank for the biological artificial liver and / or the hepatic cell protection in the long-distance transportation process.

The invention relates to exosome as well as a preparation method and application thereof in preparing medicines for treating lung cancer. The preparation method comprises a step of stimulating cells to secrete the exosome by using KRN7000 and ATP (Adenosine Triphosphate). By adopting the preparation method provided by the invention, efficient in-vitro exosome preparation can be achieved. The invention further provides the exosome prepared by using the method. The exosome has relatively good cytotoxic effects and lung tumor resistance activity. The invention further provides application of the exosome in preparing medicines for treating lung cancer.

The invention discloses a novel high-consumption resistant multi-element rare earth tungsten electrode and a preparation method thereof. The materials contain four rare earth oxides, i.e. Re2O3, La2O3, CeO2 and Y2O3 in the weight ratio of 1:2:2:6, wherein the total content of the four rare earth oxides is 1.0 to 3.0 percent of the weight percentage of total metal. The preparation method comprises the following steps of: adenosine triphosphate (ATP) doping, two-stage hydrogen reduction, spark plasma sintering (SPS), and machining the materials into electrodes with various specifications through rotary forging and drawing, wherein the rotary forging cogging temperature is 1,550 to 1,600 DEG C. The electrode is high in density, good in welding performance, reasonable in preparation process, high in material yield and obvious in energy-saving effect, and harmful gas is not generated in a using process of the electrode.

The invention relates to medicine, more precisely to hygieology and ophthalmology, and can be used for preventing deterioration of the sense of sight and also for improving the sense of sight of users reading off a screen. The invention increases the level of comfort and the reliability of the inventive method. The inventive method consists in influencing the user's eyes by additional pictures on screen, whereby an image is formed whose shape and color have a relaxing effect on a visual analyzer. The duration of exposure of the additional pictures is organized in such a way that makes it possible to restore the content of adenosine triphosphate and oxygen in the eye tissue; time intervals between exposures is less than time needed for acidulation of tissues and for the occurrence of a lack of oxygen. In the beginning the user watches such additional pictures whose shape and color range have a relaxing reflect on the visual analyzer region in the central nervous system (CNS). The shape and the color range of the additional pictures which have a relaxing effect on the visual analyzer are selected in such a way that they perform a relaxing effect on lateral and transversal eye muscles. The time interval between the images ranges from 20 to 150 min, the duration of exposure is selected within a range of 1.0 to 6.0 min. Together with the relaxing effect on a visual organ, said relaxing action is also exerted on an organ of hearing and muscles of the neck. The shape of at least a part of the additional pictures is smooth and roundish without distinct lines and boundaries.