112 results about "Adenosine triphosphate" patented technology

The invention provides a genetically engineered bacterium for producing O-succinyl-L-homoserine as well as a construction method and application of the genetically engineered bacterium. In a chassis bacterium genome, the expression of a 2-ketoglutaric acid decarboxylase encoding gene sucA is enhanced, and the expression of a succinyl-coenzyme A synthetase encoding gene sucD is weakened, so that the supply of succinyl-coenzyme A is increased; the method comprises the following steps: increasing the NADPH (Nicotinamide Adenine Dinucleotide Phosphate) reducing capacity and ATP (Adenosine Triphosphate) energy supply of a chassis bacterium, increasing DNA (Deoxyribose Nucleic Acid) in combination with a transcription dual regulatory factor ompR to improve the stress resistance of escherichia coli under high osmotic pressure, and introducing an overexpression plasmid containing a homoserine transsuccinylase coding gene metA to construct the genetically engineered bacterium for producing O-succinyl-L-homoserine. The engineering strain obtained through a systematic metabolic engineering modification strategy can realize effective accumulation of OSH, the shake flask yield of OSH reaches 19.8 g / L, the fed-batch fermentation yield of a 5L fermentation tank reaches 110.5 g / L, the sugar-acid conversion rate reaches 52.6%, and a foundation is laid for subsequent construction of high-yield OSH engineering bacteria.

The present invention relates to the field of biotechnology and discloses a probiotic composition for alleviating sarcopenic obesity and its use. The composition comprises at least two of Lactobacillus plantarum, Lactobacillus rhamnosus, and Bifidobacterium animalis subsp. lactis; wherein the Lactobacillus plantarum has a deposit number of CGMCC No. 25839; the Lactobacillus rhamnosus has a deposit number of CGMCC No. 26968; and the Bifidobacterium animalis subsp. lactis has a deposit number of CGMCC No. 27998. The composition of the present invention can increase muscle mass, improve muscle tissue morphology, increase adenosine triphosphate, and inhibit abnormal weight gain, thereby effectively preventing and treating sarcopenic obesity.

The invention discloses an ATP (adenosine triphosphate) loaded polyelectrolyte microcapsule and a preparation method thereof. Belongs to the technical field of polyelectrolyte microcapsule preparation. The polyelectrolyte microcapsule is composed of a capsule wall and a core material, wherein the capsule wall is composed of a cationic polyelectrolyte layer and an anionic polyelectrolyte layer which are alternately adsorbed; the core material is ATP molecules and can be loaded on the hollow part of the multi-layer polyelectrolyte capsule and / or the capsule wall of the multi-layer polyelectrolyte capsule. The ATP capsule provided by the invention can effectively protect the stability of ATP molecules, so that the ATP molecules are prevented from being quickly degraded in organisms, the preparation method and process of the ATP capsule are simple, the size of the capsule, the loading rate and the release rate of ATP can be regulated and controlled by changing the size of a template, the variety of the template, the adding concentration of an ATP solution, the number of layers of the capsule and the external environment, and the ATP capsule has a good application prospect. The application range of ATP in disease treatment is greatly expanded, and the use efficiency and safety of ATP are improved.

The invention relates to a dsRNA insecticide for preventing and treating thrips and a preparation method of the dsRNA insecticide. Belongs to the field of biotechnology and agricultural application. The dsRNA insecticide for preventing and treating the thrips is a preparation taking dsRNA as an effective component, and the dsRNA is of a double-stranded RNA structure containing a positive-sense strand and an antisense strand; the nucleotide sequence of the positive-sense strand is as shown in SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 5, SEQ ID NO. 7, SEQ ID NO. 9 or SEQ ID NO. 11. The invention proves that dsACT and dsVATB can cause degradation of messenger RNA (Ribonucleic Acid) of ATP (Adenosine Triphosphate) enzyme coding genes in thrips and reduce the expression level of related genes; laboratory test and field efficacy test results jointly show that after the dsRNA insecticide is sprayed, the growth and development of the thrips are affected, individual death is further caused, and the achievement lays a good theoretical and application foundation for prevention and treatment of the thrips.

The invention relates to a DNA (deoxyribonucleic acid) ligation buffer solution and application thereof, the DNA ligation buffer solution comprises 5-15 mM of divalent metal ions, 0.5-5 mM of DTT (dithiothreitol), 10-15% of polyethylene glycol, 0.5-5 mM of ATP (adenosine triphosphate) and a Tris-HCl buffer solution, and the pH (potential of hydrogen) value of the Tris-HCl buffer solution is 7-9. The DNA ligation buffer solution can significantly improve the ligation reaction efficiency, and increase ligation products in the ligation reaction and the number of molecules capable of being sequenced during library construction.

The invention provides a yeast rich in polyphosphate and application of the yeast in synthesis of adenosine triphosphate, and the content of polyphosphate is 15-35 wt% based on the dry matter mass of the yeast rich in polyphosphate; according to the method disclosed by the invention, the yeast is subjected to phosphorus starvation culture and then is subjected to phosphorus supplementary culture, so that the yeast is enriched with polyphosphates, and the yeast rich in polyphosphates is obtained; then, the yeast rich in polyphosphate is used as a biocatalyst to synthesize ATP, that is, self-related enzyme systems, mainly including adenosine kinase, endo-polyphosphate kinase and excision-polyphosphate kinase, in the yeast rich in polyphosphate are used for replacing exogenous polyphosphate kinase and adenosine kinase, and in addition, ATP is synthesized by using the yeast rich in polyphosphate as a biocatalyst. According to the method for synthesizing ATP by using the yeast rich in polyphosphate as the biocatalyst, exogenous adenosine kinase and exogenous polyphosphate kinase do not need to be additionally added, the cost is low, the efficiency of converting adenosine into ATP is high, the batch-to-batch stability of the yeast is realized, and the enzyme activity of the self-related enzyme system is high.

The invention discloses a method for catalytically synthesizing adenosine triphosphate by using microcrystalline cellulose carrier purification immobilized enzyme, which comprises the following steps: preparing bacterial suspension by using escherichia coli wet thalli and Tris-HCL buffer solution, crushing, centrifuging, and taking supernate to obtain crude enzyme; adding a microcrystalline cellulose carrier and the wet thalli into the crude enzyme liquid, and washing the immobilized enzyme after immobilization to obtain the immobilized enzyme; the method comprises the following steps: adding immobilized enzyme adenosine kinase and immobilized polyphosphate kinase into a substrate system with the volume of 1L according to a weight ratio, and starting a catalytic reaction under the conditions that the pH is 5.5-7.5 and the temperature is 20-40 DEG C; after catalysis is finished, the immobilized enzyme adenosine kinase and the immobilized polyphosphate kinase are subjected to solid-liquid separation with reaction liquid through a Buchner funnel, the immobilized enzyme continues to be subjected to catalytic reaction, and the method for preparing the immobilized enzyme is simple in mode, low in cost, easy to industrially produce, high in stability and capable of being repeatedly used for many times, and products are easy to separate.

The invention relates to a biosynthesis method of pyridoxal phosphate or pyridoxine phosphate, and belongs to the technical field of biosynthesis. In order to solve the existing problem of serious environmental pollution, the invention provides a biosynthesis method of pyridoxal phosphate or pyridoxine phosphate, which comprises the following steps: in the presence of polyphosphate, magnesium salt, ATP (adenosine triphosphate), pyridoxal kinase and polyphosphate kinase, performing phosphorylation reaction on pyridoxal or pyridoxine and acid salts thereof, thereby obtaining the pyridoxal phosphate or pyridoxine phosphate. The temperature of the phosphorylation reaction is controlled to be 30-38 DEG C, after the reaction is finished, protein separation is conducted on reaction liquid, mother liquor is separated, the compound pyridoxal phosphate or pyridoxine phosphate shown in the formula I is obtained, and the mother liquor is recycled and reused. According to the method, a large amount of ammonium acetophosphate does not need to be additionally added, operation is simpler, mother liquor can be recycled, a large amount of waste water is prevented from being discharged, environment friendliness is better achieved, and the yield is high.

The invention provides a composition for promoting ATP (adenosine triphosphate) generation and improving kidney energy metabolism by activating an AMPK / PGC-1alpha pathway. The composition is prepared from the following raw materials in parts by weight: 100 to 400 parts of herba cistanche, 50 to 200 parts of radix astragali seu hedysari, 25 to 100 parts of glycine betaine and 150 to 300 parts of alpha-ketoglutaric acid. The invention further provides a preparation method and application of the composition for improving ATP generation and improving kidney energy metabolism by activating the AMPK / PGC-1alpha pathway. Through combined use of cistanche, astragalus membranaceus, betaine and alpha-ketoglutaric acid, a multi-target synergistic mechanism of AMPK activation, metabolic substrate supplementation and epigenetic regulation is formed, the mitochondrial function and ATP generation of kidney cells are comprehensively improved, the limitation of existing single-component or chemical drugs is broken through, a safer and more efficient intervention strategy is provided for kidney energy metabolism disorders, and the clinical application prospect is broad. The invention provides an innovative solution for renal tubular energy disorder, has dual values of metabolic regulation and kidney protection, and conforms to the development trend of natural medicines. The invention belongs to the technical field of food and health care product processing.

The present invention relates to the field of enzyme engineering, and more particularly to enzyme mutants, enzyme compositions or their immobilized enzymes and their applications and methods for preparing L-carnitine. The present invention provides a simple L-carnitine holoenzyme preparation process, the overall process of which utilizes bulk betaine (N,N,N-trimethylglycine) and acetic acid as raw materials. L-carnitine is directly obtained by two-step continuous conversion by carboxyl reductase (CAR) and aldolase (DERA). This route can be completed in one step using a crude enzyme solution, or by utilizing a recyclable immobilized enzyme, and the reduced coenzyme II (NADPH) and adenosine triphosphate (ATP) used in the first step of the reaction can also be recycled using corresponding enzymes to further reduce production costs. Therefore, the patented L-carnitine preparation process has many advantages, such as being fully green, simple in process, inexpensive to produce, and easy to scale up.

The preparation method comprises the following steps: uniformly mixing a polyelectrolyte solution with positive charges and an adenosine triphosphate solution with negative charges to obtain condensed fluid drops; adding dye, biomacromolecules or nano-particles into the condensed body liquid drops to obtain condensed body liquid drops loaded with guest molecules; and incubating the condensate droplets loaded with the guest molecules, adding a sodium silicate solution, and centrifugally cleaning to obtain the condensate microspheres. According to the invention, a biomimetic mineralization method is proposed for the first time, SiO2 is used as a membrane component, condensed liquid drops are converted into solid microspheres with stable mechanical strength, and a new idea is provided for overcoming the stability bottleneck of the condensed liquid drops.

The invention relates to the technical field of biosynthesis, in particular to a biosynthesis process of adenosine triphosphate and equipment thereof. Comprising the following steps: optimizing an adenosine triphosphate synthetase gene sequence, constructing an expression vector, and introducing the expression vector into a yeast host cell; sub-packaging the engineering yeast group in a pore plate for parallel culture, detecting the acid yield and substrate conversion rate data of each micropore, and screening out elite strains; fermenting elite strains, and dynamically feeding materials by an online monitoring and feedback control system; the yeast strain is subjected to precise transformation through codon optimization and gene overexpression driven by a strong promoter; elite strains are quickly identified by adopting high-throughput screening in cooperation with miniaturized parallel fermentation and real-time optical detection; in the fermentation process, a self-control feedback fed-batch technology is introduced, and a culture environment is stabilized in an optimal physiological state through real-time monitoring and dynamic regulation, so that the production potential of engineering bacteria is fully exerted, and the yield of adenosine triphosphate, the substrate conversion rate and the product purity are improved.

The utility model discloses a solid-liquid two-in-one ATP (adenosine triphosphate) fluorescence detection swab which comprises an independently formed push rod body, a swab bracket and a sampling part, the swab bracket is of a tube body structure in which a kit is packaged, and a combined assembly part is formed at the bottom of the swab bracket; the push rod body and the swab support are assembled in a sealing mode through a piston type structure, and the lower end of the push rod body is provided with an unsealing structure aiming at the kit. The sampling part comprises a reaction tube and a sampler, the sampler comprises an independent hollow tube cotton swab and a hollow tube suction head, and the reaction tube, the hollow tube cotton swab and the hollow tube suction head can be combined and assembled through a piston type structure; when the sampler is used for detecting the swab, one of the hollow tube cotton swab and the hollow tube suction head is selected to be assembled. According to the utility model, the detection requirements in different scenes can be met, and the detection efficiency is improved, the detection accuracy is ensured, and the use cost is reduced.

The invention discloses a preparation method and application of a polysulfide compound Poly (S-[VIM-Br]) / ATP (adenosine triphosphate). The preparation method comprises the following steps: by taking industrial by-products sulfur (S8) and 1-vinyl-3-brominated imidazole onium salt (VIM-Br) as raw materials, synthesizing a multi-sulfur polymer through an anti-sulfuration reaction, and then carrying out ball-milling compounding on the multi-sulfur polymer and acidified attapulgite (ATP). The compound shows high-selectivity adsorption on precious metal Au (III) / Ag (I), the adsorption rates on Ag (I) and Au (III) in complex systems such as gold ore waste residues and waste water are as high as 99% and 93% or above, and the adsorption rate on Au (III) is kept at 76.02% after three times of circulation. The cost is reduced by using cheap raw materials and a room-temperature process; aTP modification significantly improves selectivity and stability in a complex environment; the adsorption process is accompanied by precious metal reduction (Au (III)-Au (0)), and resource recovery is facilitated.

This invention belongs to the field of target characterization technology, and provides an application of PANX2 protein and its mutants as bioporins. The bioporins can detect one or more of the following: metal ions, inorganic salts, amino acids, nucleotides, small molecule drugs, small molecule diagnostic reagents, adenosine triphosphate (ATP), and monosaccharides. In this invention, one or more amino acids of the wild-type PANX2 protein are mutated to common amino acids other than the original amino acids. Compared to the wild-type PANX2 protein, the PANX2 protein mutants can be expressed normally, are stable, and show a significantly improved current signal during detection, with a longer amplitude and fewer spikes.

The application provides a preparation method of a MOF material-based SERS substrate and application thereof, and belongs to the technical field of SERS substrate preparation. The MOF material of ZIF-8 is prepared by using a hydrothermal method, then AuNPs cores are coated in the ZIF-8 shell by using an electrostatic assembly method, and a core-shell material Au@ZIF-8 is prepared as a SERS substrate. The method for preparing the SERS substrate is simple and effective, and interference of reagents except water phase on SERS detection target small molecules is avoided. The MOF surface of the MOF material-based SERS substrate prepared by the application has uniform and adjustable size pore structures, can selectively allow only the to-be-detected small molecules to pass, and realizes detection of adenosine triphosphate in urine.

The invention discloses a luciferase mutant capable of efficiently utilizing non-natural nucleoside triphosphate. In the mutant, histidine (H) at the 244 site is mutated into tryptophan (W) on the basis of a starting enzyme Luc146-1H2 (K357E) at which amino acid No.357 is mutated from lysine (K) to glutamic acid (E). Compared with an evolutionary starting enzyme, the mutant Luc-WE not only can more efficiently utilize ATP (adenosine triphosphate) as an energy donor, but also can more efficiently utilize non-natural nucleoside triphosphate as an energy donor to catalyze the oxidation of D-fluorescein, so that a luminescence value higher than that of the evolutionary starting enzyme is generated; for different nucleoside triphosphate energy donors, the luminescence value is 2-40 times that of an evolution starting enzyme. Under the reaction condition of lower nucleoside triphosphate concentration, the luciferase mutant Luc-WE shows higher sensitivity, and has wider application range and practical value.

1. The name of the design product: adenosine triphosphate biological fluorescence detector. 2. The use of the design product: adenosine triphosphate biological fluorescence detector is used to quickly detect the amount of microorganisms and other biological residues in the sample, and to judge the health condition. It is suitable for monitoring the key control points in food and beverage production process, and for real-time sampling monitoring by medical system and health supervision institutions. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective drawing.

The present invention relates to a method and system for producing adenosine triphosphate (ATP) through an acid / aldehyde-ATP (AAA) cycle in an electrochemical cell.

The application provides a synthesis method of acetylated deoxyadenosine triphosphate, and particularly discloses that 2-chloro-1,3,2-benzodioxaphosphepin-4-ketone is used in a synthesis step, so that the synthesis of acetyl adenosine triphosphate is more efficient and stable, the conversion rate of the synthesis of acetyl adenosine triphosphate is high, and a plurality of batches of experiments prove that the conversion rate of the synthesis of acetyl adenosine triphosphate is stable and suitable for batch production.

The invention belongs to the field of insecticidal and acaricidal agents, and relates to an insecticidal and acaricidal composition. The composition contains an active component A and an active component B, and the weight part ratio of the active component A to the active component B is (1: 99)-(99: 1); the active component A is selected from a compound I; the active component B is selected from inhibitors influencing the growth of chitin synthetase 1 (CHS1) mites, mitochondrial adenosine triphosphate (ATP) synthetase inhibitors, mitochondrial electron transfer complex (III) inhibitors, mitochondrial electron transfer complex (I) inhibitors, acetyl-CoA carboxylase inhibitors and mitochondrial electron transfer complex (II) inhibitors; a trifluoroethyl sulfide type acaricide or other types of insecticidal and acaricide; the composition disclosed by the invention has the advantages of obvious synergism, resistance delay and the like, and can be used for preventing and treating various pests.

The invention relates to the technical field of preparation of functional nano materials, in particular to an MXene-coated UiO-66-coated PAP nano compound, a preparation method of the MXene-coated UiO-66-coated PAP nano compound, a flame-retardant compound and application of the flame-retardant compound. The nano-composite is obtained by loading UiO-66 onto the surface of MXene by a hydrothermal method to construct an MXene (at) UiO-66 heterostructure, and then loading dopamine hydrochloride doped adenosine triphosphate onto the surface of the MXene (at) UiO-66 through interface self-assembly. The flame-retardant compound is obtained by blending, coating and curing the nano compound, epoxy resin, a curing agent and an intumescent flame-retardant system. Through the physical barrier of MXene, the gas adsorption / catalysis of UiO-66 and the phosphorus-nitrogen synergistic flame-retardant effect of PAP, the flame retardance and smoke suppression of the flame-retardant compound are remarkably improved, the carbon residue amount reaches 39.0%, the temperature of the back face of a steel plate is reduced by 94.6 DEG C, and the flame-retardant compound is suitable for fireproof scenes such as building steel structures.

The invention discloses a meat tenderizing method for improving final tenderness by improving cell apoptosis, which is realized by injecting a disodium adenosine triphosphate solution into muscles at multiple points after a pig carcass is split into halves. According to the invention, adenosine disodium triphosphate is exogenously added, so that the energy level of muscles is improved, more muscle cells die in an apoptosis manner, and Caspase can be activated by apoptosis, so that a tenderization effect is achieved. The adenosine disodium triphosphate is colorless and odorless, and is a food additive allowed to be used in the national standard. The meat tenderizing method can maintain the original shape of the meat to a greater extent, does not influence the color of the meat, and is very good in safety at the same time.

The present invention provides a composition comprising adenosine or adenosine triphosphate (ATP) as an absorption enhancer in combination with a nutrient such as a nutrient, protein, peptide, vitamin, phytochemical, mineral, fatty acid or amino acid or a drug. The present invention describes methods of administering ATP or adenosine to improve the bioavailability of a nutrient such as a nutrient, protein, peptide, vitamin, phytochemical, mineral, fatty acid or amino acid or a drug.

The invention relates to the technical field of nano medicines, and discloses an ATP / cerium coordination polymer nano regulator as well as a preparation method and application thereof. The invention relates to an ATP / cerium coordination polymer nano regulator. The invention relates to a self-assembly hydrogel which is prepared from raw materials including cerous nitrate (III), adenosine-5 '-disodium triphosphate, phospholipid polyethylene glycol amino, SS31 peptide and a diselenide cross-linking agent through a self-assembly and cross-linking method, can effectively remove ROS (reactive oxygen species) in an inner ear and protect cells from oxidative stress injury, thereby protecting auditory cells and improving the quality of the inner ear. The traditional Chinese medicine composition has the effect of treating hearing loss caused by oxidative stress induced by noise, can be used for treating hearing loss through middle ear cavity local administration, is simple in preparation method and mild in reaction condition, and can realize large-scale production. Cascade catalysis of cerium (III) and cerium (IV), ATP (adenosine triphosphate) energy supply and a nano system of a selenium biological material are utilized, and a novel method is provided for treating noise-induced hearing loss.

To discover, among compounds derived from natural products, those having excellent action regarding liver function-improving action, and to provide liver function improvers containing them as active ingredients.SOLUTION: A liver function improver used for liver function-improving application based on hepatocellular adenosine triphosphate production-promoting action, comprising as an active ingredient a compound represented by the following formula (I), and a liver function-improving oral composition used for liver function-improving application based on hepatocellular adenosine triphosphate production-promoting action, containing a compound represented by the following formula (I).SELECTED DRAWING: None

This invention discloses a machine learning-enhanced fluorescence detection method for silver nanoclusters containing split nucleic acid aptamers, used for the detection of adenosine triphosphate (ATP) in aquatic products, belonging to the field of analytical chemistry. This detection method uses two split nucleic acid aptamers of ATP as templates to synthesize DNA-AgNCs. In the presence of the target ATP, a conformational change is induced, resulting in a decrease in fluorescence intensity. Quantitative analysis of ATP is achieved by detecting this fluorescence change. To further improve the detection effect, machine learning is introduced to preprocess the fluorescence spectrum. A recursive feature elimination algorithm is used to screen characteristic wavelengths, and a multi-dimensional feature set is constructed using maximum fluorescence intensity, peak area, and full width at half maximum (FWHM). The optimal classification model is obtained through cross-validation optimization. This invention combines aptamer recognition, DNA-AgNCs, and machine learning to construct a detection method where fluorescence dynamically decreases with increasing ATP concentration. It has advantages such as low cost, simple operation, and good biocompatibility, effectively improving detection sensitivity and stability, and has promising application prospects in the qualitative and quantitative analysis of ATP in aquatic products.

The invention belongs to the technical field of enzyme engineering, and particularly relates to a guanylate kinase mutant with improved thermal stability and application and a product thereof. Aiming at the defects that wild guanylate kinase is insufficient in thermal stability and easy to inactivate in reaction, mutants (A24Q, S75P, K146P and L172I) are obtained by site-directed mutagenesis modification based on a wild guanylate kinase sequence. The mutant is replaced by specific amino acid residues, so that the thermal stability is remarkably improved, meanwhile, good catalytic activity is kept, wider temperature conditions can be tolerated, and the effective action time of enzyme is prolonged. The mutant can efficiently catalyze a phosphate group transfer reaction of guanylic acid and adenosine triphosphate, and is suitable for enzymatic synthesis of nucleoside compounds such as guanosine diphosphate. The application of the enzyme source can reduce the use amount and supplement frequency of enzyme preparations, reduce the production cost, improve the product yield and quality, and provide reliable enzyme source support for the industrial efficient production of nucleoside products.

The present invention relates to the field of biosynthesis technology, and more particularly to a β-nicotinamide mononucleotide production system and a method for preparing β-nicotinamide mononucleotide in a dual-enzyme collaborative manner. The β-nicotinamide mononucleotide production system comprises an NRK-HS01 whole-cell catalyst, an ADK whole-cell catalyst, nicotinamide riboside, adenosine triphosphate, and MgCl2. The present invention couples the NRK-HS01 whole-cell catalyst with an ATP regeneration system (ADK whole-cell catalyst) to catalyze the conversion of nicotinamide riboside and adenosine triphosphate to produce NMN in a single step. This method achieves an NMN molar yield exceeding 99%, reduces ATP costs by 50%, and reduces feedback inhibition of the synthesis reaction caused by the accumulation of the byproduct ADP. This method also enables the recycling of ATP, thereby improving synthesis efficiency and saving production costs. This method is of great significance for the industrial development of biosynthesis of NMN.

The invention belongs to the field of biological medicine and protein engineering, and particularly relates to a preparation method of a protease cryoelectron microscope sample and a protease structure analysis method. The preparation method comprises the following steps: providing an enzyme solution of target protease, adding an enzyme inhibitor of the target protease, incubating an obtained mixture, and collecting an incubation product supernatant; and sucking the incubation product supernatant to prepare a cryoelectron microscope sample, the target protease comprises serine protease, the target protease is located on a cell membrane, or / and the target protease depends on ATP (adenosine triphosphate). According to the method, the freezing electron microscope sample is prepared after the enzyme inhibitor of the target protease is added into the enzyme liquid of the target protease for incubation, so that the problems of depolymerization of the membrane protease and instability of a protein soluble region can be solved, and a freezing electron microscope structure of the full-length membrane protease can be successfully analyzed in electron microscope observation; and accurate reference is provided for subsequent design of specific targeted drugs.