Metal organic framework Uio-66-based photoelectric sensor for detecting activity of protein kinase

Abstract

The invention provides a metal organic framework Uio-66-based photoelectric sensor for detecting the activity of protein kinase. The photoelectric sensor constructed through replacing a precious metal by a metal organic framework is provided for the first time, and comprises a conductive electrode coupled with a polypeptide, and a metal organic framework arranged on the conductive electrode, wherein the metal organic framework is loaded with a photoresponse substance; the metal framework can be specifically bound to a phosphorylated polypeptide; and the polypeptide undergoes a phosphorylation reaction in the presence of the activity of a biological enzyme to be detected and adenosine triphosphate (ATP). Photoelectric current changes with the activity of the protein kinase in visible lights, so super-sensitive and highly-accurate detection of the activity of the kinase is realized. The sensor has very high sensitivity; and enzyme inhibition experiments show that the sensor realizes efficient and sensitive detection of the activity of the kinase PKA, and meets use requirements under extremely severe conditions.

Description

Technical field: [0001] The invention relates to the field of photoelectrochemical sensors, in particular to the application of metal organic framework UiO-66 to the photoelectric sensor to detect protein kinase activity. Background technique: [0002] Kinase (PKA)-regulated protein phosphorylation plays an important role in metabolic and cellular transduction pathways. Overexpression of protein kinases can cause a variety of diseases such as tumors, diabetes, Alzheimer's disease and so on. In the field of biochemistry, the detection of kinase activity and its inhibitors can elucidate the molecular mechanism of signal transduction. In the field of clinical medicine and drug loading, the early detection of abnormal kinase expression activity is also conducive to the prevention and treatment of diseases. [0003] However, the working electrodes in existing biosensors are mainly glassy carbon electrodes or gold electrodes. Among them, glassy carbon electrodes cannot be directl...

AI Technical Summary

Problems solved by technology

However, DNA probes and nano-noble metal particles are used as the main devices in this design, resulting in high cost and difficulty in promotion; at the same time, the DNA chain and phosphorylated kemptide are only connected by metal ions, and the binding strength is poor, and DNA is biological Molecules, sensitive to the environment, resulting in poor stability of the sensor when used under extreme conditions

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